LOCUS       sequence-310365-         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Allows for constituitive mammalian expression of the MERS-CoV 3CL 
            protease with C145A mutation via transfection or lentivirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Resnick SJ, Iketani S, Hong SJ, Zask A, Liu H, Kim S, Melore S, Lin 
            FY, Nair MS, Huang Y, Lee S, Tay NES, Rovis T, Yang HW, Xing L, 
            Stockwell BR, Ho DD, Chavez A
  TITLE     Inhibitors of Coronavirus 3CL Proteases Protect Cells from 
            Protease-Mediated Cytotoxicity.
  JOURNAL   J Virol. 2021 Jun 24;95(14):e0237420. doi: 10.1128/JVI.02374-20. 
            Epub 2021 Jun 24.
  PUBMED    33910954
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "2021-06-24"; volume: "95(14):e0237420. doi"; pages: " 
            10.1128/JVI.02374-20. Epub 2021 Jun 24"
COMMENT     Sequence Label: pLEX307-MERS-CoV-3CL-C148A
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgagcggtt tggtgaaaat gtcacatccc agtggagatg ttgaggcttg tatggttcag
       61 gttacctgcg gtagcatgac tcttaatggt ctttggcttg acaacacagt ctggtgccca
      121 cgacacgtaa tgtgcccggc tgaccagttg tctgatccta attatgatgc cttgttgatt
      181 tctatgacta atcatagttt cagtgtgcaa aaacacattg gcgctccagc aaacttgcgt
      241 gttgttggtc atgccatgca aggcactctt ttgaagttga ctgtcgatgt tgctaaccct
      301 agcactccag cctacacttt tacaacagtg aaacctggcg cagcatttag tgtgttagca
      361 tgctataatg gtcgtccgac tggtacattc actgttgtaa tgcgccctaa ctacacaatt
      421 aagggttcct ttctgtgtgg ttctgccggt agtgttggtt acaccaagga gggtagtgtg
      481 atcaatttct gttacatgca tcaaatggaa cttgctaatg gtacacatac cggttcagca
      541 tttgatggta ctatgtatgg tgcctttatg gataaacaag tgcaccaagt tcagttaaca
      601 gacaaatact gcagtgttaa tgtagtagct tggctttacg cagcaatact taatggttgc
      661 gcttggtttg taaaacctaa tcgcactagt gttgtttctt ttaatgaatg ggctcttgcc
      721 aaccaattca ctgaatttgt tggcactcaa tccgttgaca tgttagctgt caaaacaggc
      781 gttgctattg aacagctgct ttatgcgatc caacaactgt atactgggtt ccagggaaag
      841 caaatccttg gcagtaccat gttggaagat gaattcacac ctgaggatgt taatatgcag
      901 attatgggtg tggttatgca gtaa
//
