LOCUS       sequence-310367-         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Allows for constituitive mammalian expression of the Bat-CoV-HKU9 
            3CL protease with C145A mutation via transfection or lentivirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Resnick SJ, Iketani S, Hong SJ, Zask A, Liu H, Kim S, Melore S, Lin 
            FY, Nair MS, Huang Y, Lee S, Tay NES, Rovis T, Yang HW, Xing L, 
            Stockwell BR, Ho DD, Chavez A
  TITLE     Inhibitors of Coronavirus 3CL Proteases Protect Cells from 
            Protease-Mediated Cytotoxicity.
  JOURNAL   J Virol. 2021 Jun 24;95(14):e0237420. doi: 10.1128/JVI.02374-20. 
            Epub 2021 Jun 24.
  PUBMED    33910954
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "2021-06-24"; volume: "95(14):e0237420. doi"; pages: " 
            10.1128/JVI.02374-20. Epub 2021 Jun 24"
COMMENT     Sequence Label: pLEX307-Bat-CoV-HKU9-3CL-C144A
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggccggcc tgacacgtat ggctcaccct tcaggtttag tagagccgtg ccttgttaaa
       61 gtaaattatg gttccatgac tcttaatggt atatggttgg ataattttgt tatatgtcct
      121 aggcatgtta tgtgttctag ggatgagtta gctaatcctg attaccctcg tttgtctatg
      181 cgagctgcta attatgattt tcacgtgtct caaaatggtc ataatattcg tgttataggc
      241 catactatgg aaggttcgct tttaaagcta acagttgatg tgaataatcc taaaacaccc
      301 gcttattcat ttatacgggt gagtacgggt caagctatga gtttgttggc atgttatgat
      361 ggtttaccaa ctggtgtgta tacgtgcact ttacggtcga atggtactat gagagcatca
      421 tttttatgtg gctctgccgg tagtcctggc tttgtcatga atggcaaaga agttcaattt
      481 tgttacctac accagcttga attaccaaat ggtactcata ctggtacaga tttttctggt
      541 gtcttttatg gtccatttga agacaagcaa gtgcctcaat tagcggcgcc tgattgtact
      601 ataactgtta atgttttagc atggctttat gcagctgtgt taagtggtga gaattggttt
      661 ttaaccaagt ctagtatttc accggctgaa tttaataatt gtgctgttaa gtacatgtgt
      721 cagtcagtaa cgagtgaaag cttgcaagtt ttgcaaccac ttgcagctaa aacaggtatc
      781 tctgttgagc gcatgctttc agcgttgaag gtattgctct cagctggatt ttgtggccgt
      841 actattatgg gctcctgttc tttagaggat gagcatacac cgtatgatat tggccgtcaa
      901 atgttaggtg ttaaattgca ataa
//
