LOCUS       sequence-310369-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Allows for constituitive mammalian expression of the HCoV-NL63 3CL 
            protease with C144A mutation via transfection or lentivirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Resnick SJ, Iketani S, Hong SJ, Zask A, Liu H, Kim S, Melore S, Lin 
            FY, Nair MS, Huang Y, Lee S, Tay NES, Rovis T, Yang HW, Xing L, 
            Stockwell BR, Ho DD, Chavez A
  TITLE     Inhibitors of Coronavirus 3CL Proteases Protect Cells from 
            Protease-Mediated Cytotoxicity.
  JOURNAL   J Virol. 2021 Jun 24;95(14):e0237420. doi: 10.1128/JVI.02374-20. 
            Epub 2021 Jun 24.
  PUBMED    33910954
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "2021-06-24"; volume: "95(14):e0237420. doi"; pages: " 
            10.1128/JVI.02374-20. Epub 2021 Jun 24"
COMMENT     Sequence Label: pLEX307-HCoV-NL63-3CL-C144A
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgtctggtc ttaagaagat ggcacaacca tctggttgtg ttgagagatg tgtggttcgc
       61 gtctgttatg gtagtactgt gcttaatgga gtttggttag gtgacactgt tacttgtcct
      121 agacatgtca tagcaccatc aaccactgtt cttattgatt atgatcatgc atatagtact
      181 atgcgtttgc ataatttttc agtgtcttat aatggtgtct tcttgggagt tgttggtgtt
      241 acaatgcatg gttctgtgtt gcgtattaag gtttcacaat ctaatgtaca tacacctaaa
      301 catgttttta aaacgttgaa acctggtgat tcttttaata ttttagcatg ttatgaaggt
      361 attgcatctg gtgtttttgg tgttaattta cgtacaaact ttactattaa aggttctttt
      421 ataaatggag ctgccggttc tcctggttat aatgttagaa atgatggtac tgttgagttt
      481 tgttatttac accaaattga gttaggtagt ggtgctcatg ttggttctga ttttactggt
      541 agtgtttatg gtaattttga tgaccaacct agtttgcaag ttgagagtgc caaccttatg
      601 ctatcagata atgttgttgc ctttttgtat gctgctttgt tgaatggttg taggtggtgg
      661 ttgcgttcaa ctagagttaa tgttgatggt tttaatgaat gggctatggc taatggttat
      721 acaagtgttt ctagtgttga gtgctattct attttggcag caaaaactgg tgttagtgtt
      781 gaacaattgt tagcttccat tcaacatctt catgaaggtt ttggtggtaa aaacatactt
      841 ggttattcta gtttatgtga tgagttcaca ctagctgaag ttgtgaagca gatgtatggt
      901 gttaacttgc aataa
//
