LOCUS       sequence-310371-         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Allows for constituitive mammalian expression of the IBV 3CL 
            protease with C143A mutation via transfection or lentivirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Resnick SJ, Iketani S, Hong SJ, Zask A, Liu H, Kim S, Melore S, Lin 
            FY, Nair MS, Huang Y, Lee S, Tay NES, Rovis T, Yang HW, Xing L, 
            Stockwell BR, Ho DD, Chavez A
  TITLE     Inhibitors of Coronavirus 3CL Proteases Protect Cells from 
            Protease-Mediated Cytotoxicity.
  JOURNAL   J Virol. 2021 Jun 24;95(14):e0237420. doi: 10.1128/JVI.02374-20. 
            Epub 2021 Jun 24.
  PUBMED    33910954
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "2021-06-24"; volume: "95(14):e0237420. doi"; pages: " 
            10.1128/JVI.02374-20. Epub 2021 Jun 24"
COMMENT     Sequence Label: pLEX307-IBV-3CL-C143A
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggctggtt ttaagaaact agttgctcct agtagtgctg ttgagaagtg cattgttagt
       61 gtctcttata gaggcagtaa tcttaatgga ttgtggttgg gtgattccat ctactgtcca
      121 cgacatgtat taggtaagtt tagtggtgac caatggagtg atgtacttag ccttgctaat
      181 aatcatgaat ttgaggttgt aactcataat ggtgttactt tgaatgttgt cagcaggcgt
      241 ttaaagggtg cattactgat tttacagact gcagtagcca atgctgatac accgaagtat
      301 aaatttttga aagcaaattg tggtgatagt ttcacaatag cttgctctta tggtggtaca
      361 gttataggac tctaccccgt tactatgcgt tctaatggaa ctattagagc atcgtttctt
      421 gctggagcag ccggttcagt aggttttaat atagaaaagg gtgtagtaaa tttttactac
      481 atgcaccatc tagagttacc taatgcatta cacacaggaa ctgacctaat gggtgagttt
      541 tatggtggtt atatagatga agaggttgct cagaaagttc aacccgataa attagttact
      601 aataatattt tggcatggct ttatgcagca attattagtg ttaaagagag tagtttttca
      661 acaccaaaat ggcttgaaag tactactatc agtattgatg attataataa gtgggcaggt
      721 gataatggtt ttacatcatt tgtaagctgc actgctatta ctaaattaag tgctataaca
      781 ggagtagatg tttgtaaact ccttcgtact attatggtaa aaagtgcaca atggggtagt
      841 gaccctattt tgggacaata taattttgag gatgaaatga caccggaatc tgtttttaat
      901 caggtaggtg gtgttaggtt acaataa
//
