LOCUS       sequence-31039-e         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  TITLE     The Parkinson Disease-associated Leucine-rich Repeat Kinase 2 
            (LRRK2) Is a Dimer That Undergoes Intramolecular Autophosphorylation
  JOURNAL   J Biol Chem. 2008 Jun 13;283(24):16906-14.
  PUBMED    18397888
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2008-06-13"; volume: "283(24)"; pages: "16906-14"
COMMENT     Sequence Label: 2XMyc-LRRK2-RCK-Y1699C
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        40..58
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      90..99
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             99..128
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             144..173
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    192..216
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgattacgc caagctcgaa attaaccctc actaaaggga
       61 acaaaagctg gagctccacc gcggtggcgg ccgccatgga gcagaaactc atctctgaag
      121 aggatctgag cccgggcgga tccgagcaga aactcatctc tgaagaggat ctgctgcagg
      181 aattcgatat cacaagtttg tacaaaaaag caggctccga attcgccctt gctgtgcctt
      241 ataaccgaat gaaacttatg attgtgggaa atactgggag tggtaaaacc accttattgc
      301 agcaattaat gaaaaccaag aaatcagatc ttggaatgca aagtgccaca gttggcatag
      361 atgtgaaaga ctggcctatc caaataagag acaaaagaaa gagagatctc gtcctaaatg
      421 tgtgggattt tgcaggtcgt gaggaattct atagtactca tccccatttt atgacgcagc
      481 gagcattgta ccttgctgtc tatgacctca gcaagggaca ggctgaagtt gatgccatga
      541 agccttggct cttcaatata aaggctcgcg cttcttcttc ccctgtgatt ctcgttggca
      601 cacatttgga tgtttctgat gagaagcaac gcaaagcctg catgagtaaa atcaccaagg
      661 aactcctgaa taagcgaggg ttccctgcca tacgagatta ccactttgtg aatgccaccg
      721 aggaatctga tgctttggca aaacttcgga aaaccatcat aaacgagagc cttaatttca
      781 agatccgaga tcagcttgtt gttggacagc tgattccaga ctgctatgta gaacttgaaa
      841 aaatcatttt atcggag
//
