LOCUS       sequence-310392-       13281 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible dual fluorescent ratiometric binary 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13281)
  AUTHORS   Khosla A, Rodriguez-Furlan C, Kapoor S, Van Norman JM, Nelson DC
  TITLE     A series of dual-reporter vectors for ratiometric analysis of 
            protein abundance in plants.
  JOURNAL   Plant Direct. 2020 Jun 21;4(6):e00231. doi: 10.1002/pld3.231. 
            eCollection 2020 Jun.
  PUBMED    32582876
REFERENCE   2  (bases 1 to 13281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2020-06-21"; volume: "4(6):e00231. doi"; pages: " 
            10.1002/pld3.231. eCollection 2020 Jun"
COMMENT     Sequence Label: pRATIO3212
FEATURES             Location/Qualifiers
     source          1..13281
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    361..485
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(522..527,528..545,546..552)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             606..1265
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(672..691)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1607..1912
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1791..1810
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1953..2077)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2877..2942
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             join(2949..2951,2952..2954,2955..3662)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(2994..3015)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3255..3274)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3602..3623
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      3697..3949
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3998..4014)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3998..4014)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4011..4033)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4022..4038
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4046..4052,4053..4070,4071..4076))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4091..4112
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(4146..4398)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4788..5582)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4899..4918)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5509..5528
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(5603..5786)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(5639..5818)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    5992..6016
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6537..7328
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7574..8162
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8063..8082
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8316..8333
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8348..8488
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8414..8433)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8574..8596
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8832..9026
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9092..10165)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10594..11223)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    12524..12548
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     12736..12758
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12750..12767
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12751..12767
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactctagt
      361 acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta
      421 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca
      481 ctatggcggc ccctcgaggg gccgcattag gcaccccagg ctttacactt tatgcttccg
      541 gctcgtataa tgtgtggatt ttgagttagg atccggcgag attttcagga gctaaggaag
      601 ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta
      661 aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc
      721 tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct
      781 ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag
      841 acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa
      901 ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca
      961 tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta
     1021 ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa
     1081 acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc
     1141 aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtc tgtgatggct
     1201 tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg
     1261 cgtaaagatc tggatccggc ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg
     1321 atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca aaaagaggtg
     1381 tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt tgctcaaggc
     1441 atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga agcccgtcgt
     1501 ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt cgcccggttt
     1561 attgaaatga acggctcttt tgctgacgag aacagggact ggtgaaatgc agtttaaggt
     1621 ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat
     1681 tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa
     1741 agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac
     1801 caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca
     1861 ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg
     1921 ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt tgtgttttac
     1981 agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat
     2041 tttacgtttc tcgttcagct ttcttgtaca aagtggtggg cccaatggtg tccaagggcg
     2101 aagctgtgat taaagaattt atgcgtttta aggtccacat ggaaggatcc atgaatggac
     2161 atgaattcga aatcgagggg gagggcgagg gccgacctta tgagggtaca cagaccgcga
     2221 agctaaaggt aaccaaggga ggcccactac cctttagttg ggacatactc agcccgcagt
     2281 ttatgtatgg cagtcgtgcc tttataaagc acccagcgga tataccagac tactacaaac
     2341 agtccttccc agaggggttt aaatgggagc gagtgatgaa ctttgaggac ggtggtgctg
     2401 tgacggtaac tcaggacaca tcactcgaag acgggactct tatttacaaa gtaaaactga
     2461 gagggaccaa ttttccacca gatggccccg tcatgcaaaa aaagacgatg ggttgggaag
     2521 catctacaga acgactctat cccgaggacg gcgtcctcaa aggcgacatt aaaatggcct
     2581 tgaggttgaa agacggtgga cgttacttgg cggacttcaa aacaacttat aaagcaaaaa
     2641 agccagtgca aatgcctgga gcgtacaatg tagatagaaa acttgatata acgtcacata
     2701 atgaggacta taccgtggtt gagcaatacg agaggtcaga aggacgacac agcaccggcg
     2761 gtatggacga gttgtacaag acgcgttcta atgccgccga tgaggttgcg actggatcag
     2821 gtcttttagc gatacatccg accgaagcgc gacataaaca aaaaattgta gcgccggtca
     2881 aacaaaccct gaatttcgac ttactcaaac ttgcgggtga cgtagagtca aatccagggc
     2941 ctggtaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     3001 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     3061 ccacctacgg caagctgacc ctgaagctga tctgcaccac cggcaagctg cccgtgccct
     3121 ggcccaccct cgtgaccacc ctgggctacg gcctgcagtg cttcgcccgc taccccgacc
     3181 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     3241 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     3301 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     3361 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcacc gccgacaagc
     3421 agaagaacgg catcaaggcc aacttcaaga tccgccacaa catcgaggac ggcggcgtgc
     3481 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     3541 acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     3601 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     3661 acattgctgc agcggcctaa gagctcgaat ttccccgatc gttcaaacat ttggcaataa
     3721 agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg
     3781 aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt
     3841 tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc
     3901 gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg ggaattggtt
     3961 ccggaaccaa ttggttccgg aaccaattcg taatcatgtc atagctgttt cctgtgtgaa
     4021 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     4081 ggggtgccta atgagtgagc taactcacat taggctgaat taggcgcgcc tatttctgaa
     4141 ttcccgatct agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta
     4201 tattttgttt tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca
     4261 tctcataaat aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag
     4321 aaattatatg ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg
     4381 ccaaatgttt gaacgatcgg ggatcatccg ggtctgtggc gggaactcca cgaaaatatc
     4441 cgaacgcagc aagatatcgc ggtgcatctc ggtcttgcct gggcagtcgc cgccgacgcc
     4501 gttgatgtgg acgccgggcc cgatcatatt gtcgctcagg atcgtggcgt tgtgcttgtc
     4561 ggccgttgct gtcgtaatga tatcggcacc ttcgaccgcc tgttccgcag agatcccgtg
     4621 ggcgaagaac tccagcatga gatccccgcg ctggaggatc atccagccgg cgtcccggaa
     4681 aacgattccg aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat ctcgtgatgg
     4741 caggttgggc gtcgcttggt cggtcatttc gaaccccaga gtcccgctca gaagaactcg
     4801 tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg
     4861 aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct
     4921 atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg
     4981 ccattttcca ccatgatatt cggcaagcag gcatcgccat gggtcacgac gagatcatcg
     5041 ccgtcgggca tgcgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc
     5101 tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg
     5161 atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc
     5221 cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga
     5281 tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg
     5341 agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtcc
     5401 tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc
     5461 gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag
     5521 ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc
     5581 atgcgaaacg atccagatcc ggtgcagatt atttggattg agagtgaata tgagactcta
     5641 attggatacc gaggggaatt tatggaacgt cagtggagca tttttgacaa gaaatatttg
     5701 ctagctgata gtgaccttag gcgacttttg aacgcgcaat aatggtttct gacgtatgtg
     5761 cttagctcat taaactccag aaacccgcgg ctgagtggct ccttcaacgt tgcggttctg
     5821 tcagttccaa acgtaaaacg gcttgtcccg cgtcatcggc gggggtcata acgtgactcc
     5881 cttaattctc cgctcatgat gaattcagaa ataaattcag cctaattcgg cgttaattca
     5941 gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc
     6001 acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc
     6061 accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag
     6121 gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg
     6181 tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt
     6241 gctgcctgtg atcaattcgg gcacgaaccc agtggacata agcctgttcg gttcgtaagc
     6301 tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
     6361 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt
     6421 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat
     6481 ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatgg
     6541 gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc
     6601 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc
     6661 tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa
     6721 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga
     6781 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc
     6841 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct
     6901 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata
     6961 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc
     7021 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg
     7081 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa
     7141 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc
     7201 ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc
     7261 gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg
     7321 gcaaataatg tctagctaga aattcgttca agccgacgcc gcttcgcggc gcggcttaac
     7381 tcaagcgtta gatgcactaa gcacataatt gctcacagcc aaactatcag gtcaagtctg
     7441 cttttattat ttttaagcgt gcataataag ccctacacaa attgggagat atatcatgca
     7501 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     7561 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     7621 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     7681 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     7741 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     7801 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     7861 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     7921 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     7981 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     8041 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     8101 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     8161 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     8221 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     8281 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     8341 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
     8401 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct
     8461 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc
     8521 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     8581 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagg gtgccttgat
     8641 gtgggcgccg gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt agattgcctg
     8701 gccgtaggcc agccattttt gagcggccag cggccgcgat aggccgacgc gaagcggcgg
     8761 ggcgtaggga gcgcagcgac cgaagggtag gcgctttttg cagctcttcg gctgtgcgct
     8821 ggccagacag ttatgcacag gccaggcggg ttttaagagt tttaataagt tttaaagagt
     8881 tttaggcgga aaaatcgcct tttttctctt ttatatcagt cacttacatg tgtgaccggt
     8941 tcccaatgta cggctttggg ttcccaatgt acgggttccg gttcccaatg tacggctttg
     9001 ggttcccaat gtacgtgcta tccacaggaa agagaccttt tcgacctttt tcccctgcta
     9061 gggcaatttg ccctagcatc tgctccgtac attaggaacc ggcggatgct tcgccctcga
     9121 tcaggttgcg gtagcgcatg actaggatcg ggccagcctg ccccgcctcc tccttcaaat
     9181 cgtactccgg caggtcattt gacccgatca gcttgcgcac ggtgaaacag aacttcttga
     9241 actctccggc gctgccactg cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct
     9301 tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa
     9361 agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca
     9421 tccaatcagc tagctcgatc tcgatgtact ccggccgccc ggtttcgctc tttacgatct
     9481 tgtagcggct aatcaaggct tcaccctcgg ataccgtcac caggcggccg ttcttggcct
     9541 tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt
     9601 cgtctttctg ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg
     9661 gacggaacac gcggccgggc ttgtctccct tcccttcccg gtatcggttc atggattcgg
     9721 ttagatggga aaccgccatc agtaccaggt cgtaatccca cacactggcc atgccggccg
     9781 gccctgcgga aacctctacg tgcccgtctg gaagctcgta gcggatcacc tcgccagctc
     9841 gtcggtcacg cttcgacaga cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg
     9901 tgcccacgtc atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct
     9961 tcctaatcga cggcgcaccg gctgccggcg gttgccggga ttctttgcgg attcgatcag
    10021 cggccgcttg ccacgattca ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg
    10081 cctgcgcggc cttcaacttc tccaccaggt catcacccag cgccgcgccg atttgtaccg
    10141 ggccggatgg tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc cagtgccatt
    10201 gcagggccgg cagacaaccc agccgcttac gcctggccaa ccgcccgttc ctccacacat
    10261 ggggcattcc acggcgtcgg tgcctggttg ttcttgattt tccatgccgc ctcctttagc
    10321 cgctaaaatt catctactca tttattcatt tgctcattta ctctggtagc tgcgcgatgt
    10381 attcagatag cagctcggta atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg
    10441 tgtgatcctc cgccggcaac tgaaagttga cccgcttcat ggctggcgtg tctgccaggc
    10501 tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg
    10561 tgcttttgct cattttctct ttacctcatt aactcaaatg agttttgatt taatttcagc
    10621 ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt tctgattcaa gaacggttgt
    10681 gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg atacgggact caagaatggg
    10741 cagctcgtac ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc
    10801 ccgcgacacg acaaaggccg cttgtagcct tccatccgtg acctcaatgc gctgcttaac
    10861 cagctccacc aggtcggcgg tggcccatat gtcgtaaggg cttggctgca ccggaatcag
    10921 cacgaagtcg gctgccttga tcgcggacac agccaagtcc gccgcctggg gcgctccgtc
    10981 gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc
    11041 gatgccgaca acggttagcg gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc
    11101 ctggggatcg gaatcgacta acagaacatc ggccccggcg agttgcaggg cgcgggctag
    11161 atgggttgcg atggtcgtct tgcctgaccc gcctttctgg ttaagtacag cgataacctt
    11221 catgcgttcc ccttgcgtat ttgtttattt actcatcgca tcatatacgc agcgaccgca
    11281 tgacgcaagc tgttttactc aaatacacat caccttttta gacggcggcg ctcggtttct
    11341 tcagcggcca agctggccgg ccaggccgcc agcttggcat cagacaaacc ggccaggatt
    11401 tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga acacgtaccc ggccgcgatc
    11461 atctccgcct cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt
    11521 ttcatgcttg ttcctcttgg cgttcattct cggcggccgc cagggcgtcg gcctcggtca
    11581 atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc
    11641 gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc gcctctttca
    11701 cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg
    11761 tagggcgggg gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc
    11821 ggtcgatgat tagggaacgc tcgaactcgg caatgccggc gaacacggtc aacaccatgc
    11881 ggccggccgg cgtggtggtg tcggcccacg gctctgccag gctacgcagg cccgcgccgg
    11941 cctcctggat gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta
    12001 gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg gccagctccg
    12061 ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag cttggtgcag ccggccgcgt
    12121 gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca
    12181 gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat
    12241 tctactttat gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag
    12301 cctgtcgcgt aacttaggac ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac
    12361 gtcagaagcc gactgcacta tagcagcgga ggggttggat caaagtactt tgatcccgag
    12421 gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct tttcacgccc
    12481 ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc cgccaatata
    12541 tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga tccaagctca
    12601 agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
    12661 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
    12721 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagcttgcat
    12781 gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc cacagatggt
    12841 tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata atctccagga
    12901 aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaactgcat
    12961 caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag tatggacgat
    13021 tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta aaaaggtagt
    13081 tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa cagaactcgc
    13141 cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca agaagaaaat
    13201 cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca aagatacagt
    13261 ctcagaagac caaagggcaa t
//
