LOCUS       sequence-310395-       14039 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible dual bioluminescent ratiometric binary 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14039)
  AUTHORS   Khosla A, Rodriguez-Furlan C, Kapoor S, Van Norman JM, Nelson DC
  TITLE     A series of dual-reporter vectors for ratiometric analysis of 
            protein abundance in plants.
  JOURNAL   Plant Direct. 2020 Jun 21;4(6):e00231. doi: 10.1002/pld3.231. 
            eCollection 2020 Jun.
  PUBMED    32582876
REFERENCE   2  (bases 1 to 14039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2020-06-21"; volume: "4(6):e00231. doi"; pages: " 
            10.1002/pld3.231. eCollection 2020 Jun"
COMMENT     Sequence Label: pRATIO3267
FEATURES             Location/Qualifiers
     source          1..14039
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    361..485
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(522..527,528..545,546..552)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             606..1265
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(672..691)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1607..1912
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1791..1810
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1953..2077)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3825..3890
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     terminator      4471..4723
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4756..4772)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4756..4772)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4769..4791)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4780..4796
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4804..4810,4811..4828,4829..4834))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4849..4870
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(4904..5156)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5546..6340)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5657..5676)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6267..6286
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(6361..6544)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(6397..6576)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6750..6774
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7295..8086
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8332..8920
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8821..8840
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9074..9091
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9106..9246
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9172..9191)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9332..9354
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9590..9784
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9850..10923)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11352..11981)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    13282..13306
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     13494..13516
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     13508..13525
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     13509..13525
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactctagt
      361 acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta
      421 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca
      481 ctatggcggc ccctcgaggg gccgcattag gcaccccagg ctttacactt tatgcttccg
      541 gctcgtataa tgtgtggatt ttgagttagg atccggcgag attttcagga gctaaggaag
      601 ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta
      661 aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc
      721 tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct
      781 ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag
      841 acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa
      901 ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca
      961 tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta
     1021 ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa
     1081 acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc
     1141 aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtc tgtgatggct
     1201 tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg
     1261 cgtaaagatc tggatccggc ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg
     1321 atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca aaaagaggtg
     1381 tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt tgctcaaggc
     1441 atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga agcccgtcgt
     1501 ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt cgcccggttt
     1561 attgaaatga acggctcttt tgctgacgag aacagggact ggtgaaatgc agtttaaggt
     1621 ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat
     1681 tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa
     1741 agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac
     1801 caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca
     1861 ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg
     1921 ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt tgtgttttac
     1981 agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat
     2041 tttacgtttc tcgttcagct ttcttgtaca aagtggtggg cccaatggag aatatggaga
     2101 acgacgagaa tattgttgtc gggcctaaac ctttttaccc gatagaagag gggtctgccg
     2161 ggactcagtt aagaaaatac atggaacgtt atgccaaact tggtgccatc gcgttcacga
     2221 atgctgtgac cggggtagat tactcttacg ccgaatatct tgaaaaaagc tgctgtctag
     2281 ggaaggccct acaaaactac gggcttgttg tcgacgggag gatcgcacta tgttctgaaa
     2341 attgcgaaga gttctttatc ccagtaatcg cggggctatt tattggcgtc ggggtggcgc
     2401 ccaccaacga aatatacaca ctacgagagc ttgtccacag cctagggatc tctaaaccca
     2461 ctatcgtatt ctcatctaag aagggactcg acaaggtaat tacagtgcaa aagaccgtta
     2521 cgaccatcaa aacaattgtc atactcgaca gtaaagtaga ctacaggggc tatcagtgcc
     2581 tcgacacgtt tattaagcga aacaccccac caggttttca ggcttccagc ttcaagactg
     2641 tggaagttga tcgaaaggaa caagtggcgc tcatcatgaa ctccagcggc tcaacgggtc
     2701 tccccaaggg cgttcaactt actcacgaaa acacggtaac ccgttttagc catgcacgag
     2761 acccgatcta cggcaaccag gtaagccctg gcaccgctgt cttaacagtg gttcctttcc
     2821 atcatgggtt tggaatgttc accacgctag gatatctaat ttgcggcttt agagttgtga
     2881 tgttgacaaa gtttgatgag gaaacttttc taaaaactct acaggactac aaatgcacat
     2941 atgtgatttt ggttccaacg cttttcgcca ttcttaataa atcagaactg ttgaacaagt
     3001 acgatctcag taacttggtg gagatcgctt ctgggggtgc ccccctgtct aaggaagtag
     3061 gagaggcagt ggctcgtcgt tttaacttac cgggggtccg tcaaggatac ggtttgacgg
     3121 aaacgacatc cgccatcatc attacgccag agggcgacga caaacctgga gctagtggta
     3181 aagttgtgcc tttatttaag gcgaaggtaa ttgacctaga cacgaagaag agtctaggtc
     3241 ctaataggag aggggaggta tgcgtcaagg gacccatgct gatgaaaggt tatgttaata
     3301 acccggaagc gactaaggaa ttgattgacg aggaaggttg gttgcatacg ggagatatag
     3361 gttattatga cgaggaaaaa cactttttca tagtagacag gctgaagtca ttgattaaat
     3421 acaaagggta tcaagtccca cccgccgagc ttgaatctgt tcttcttcaa catcctagca
     3481 tttttgacgc tggggttgcg ggagttcccg atccggttgc gggagagctg ccaggtgcag
     3541 tagttgtgtt agagtccggg aagaatatga ctgaaaagga agtgatggac tatgtggcaa
     3601 gtcaagtctc caacgccaag agactgagag gtggcgtccg attcgttgac gaagttccca
     3661 aggggctaac aggcaagatt gacgggcgtg caatcagaga gatattaaag aaacccgtgg
     3721 ccaaaatgac gcgttctaat gccgccgatg aggttgcgac tggatcaggt cttttagcga
     3781 tacatccgac cgaagcgcga cataaacaaa aaattgtagc gccggtcaaa caaaccctga
     3841 atttcgactt actcaaactt gcgggtgacg tagagtcaaa tccagggcct ggtaccatgg
     3901 gtgtcaaagt gctattcgct ttgatctgca tcgccgttgc agaagcaaaa cccaccgaaa
     3961 ataatgagga ctttaacata gtcgcggtag cgtctaactt tgccactacg gatttggacg
     4021 ctgacagagg caagctgccg ggtaaaaaac ttccgctgga agttctgaaa gagcttgagg
     4081 ctaatgcacg taaggcaggc tgcaccagag ggtgcctaat ttgcttatca cacataaagt
     4141 gtacacccaa gatgaagaag tttattcccg gaagatgcca tacatacgaa ggtgacaaag
     4201 agtccgccca aggaggtata ggcgaagcca tcgttgacat accagagatc ccaggattca
     4261 aggacttaga gcctttagag caattcattg ctcaggtaga tctctgcgta gactgcacaa
     4321 ctgggtgcct aaagggcttg gcaaacgtcc aatgctcaga cttattgaag aaatggttac
     4381 cgcagagatg tgcaaccttt gcctcaaaaa tacagggtca ggtagacaaa ataaagggag
     4441 caggtggtga ctaagagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc
     4501 ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac
     4561 gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg
     4621 attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac
     4681 taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt ggttccggaa
     4741 ccaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     4801 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     4861 actcacatta ggctgaatta ggcgcgccta tttctgaatt cccgatctag taacatagat
     4921 gacaccgcgc gcgataattt atcctagttt gcgcgctata ttttgttttc tatcgcgtat
     4981 taaatgtata attgcgggac tctaatcata aaaacccatc tcataaataa cgtcatgcat
     5041 tacatgttaa ttattacatg cttaacgtaa ttcaacagaa attatatgat aatcatcgca
     5101 agaccggcaa caggattcaa tcttaagaaa ctttattgcc aaatgtttga acgatcgggg
     5161 atcatccggg tctgtggcgg gaactccacg aaaatatccg aacgcagcaa gatatcgcgg
     5221 tgcatctcgg tcttgcctgg gcagtcgccg ccgacgccgt tgatgtggac gccgggcccg
     5281 atcatattgt cgctcaggat cgtggcgttg tgcttgtcgg ccgttgctgt cgtaatgata
     5341 tcggcacctt cgaccgcctg ttccgcagag atcccgtggg cgaagaactc cagcatgaga
     5401 tccccgcgct ggaggatcat ccagccggcg tcccggaaaa cgattccgaa gcccaacctt
     5461 tcatagaagg cggcggtgga atcgaaatct cgtgatggca ggttgggcgt cgcttggtcg
     5521 gtcatttcga accccagagt cccgctcaga agaactcgtc aagaaggcga tagaaggcga
     5581 tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc
     5641 cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca
     5701 cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg
     5761 gcaagcaggc atcgccatgg gtcacgacga gatcatcgcc gtcgggcatg cgcgccttga
     5821 gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat
     5881 cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt
     5941 cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg
     6001 atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca
     6061 atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc
     6121 ccgtcgtggc cagccacgat agccgcgctg cctcgtcctg cagttcattc agggcaccgg
     6181 acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg
     6241 catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag
     6301 cggccggaga acctgcgtgc aatccatctt gttcaatcat gcgaaacgat ccagatccgg
     6361 tgcagattat ttggattgag agtgaatatg agactctaat tggataccga ggggaattta
     6421 tggaacgtca gtggagcatt tttgacaaga aatatttgct agctgatagt gaccttaggc
     6481 gacttttgaa cgcgcaataa tggtttctga cgtatgtgct tagctcatta aactccagaa
     6541 acccgcggct gagtggctcc ttcaacgttg cggttctgtc agttccaaac gtaaaacggc
     6601 ttgtcccgcg tcatcggcgg gggtcataac gtgactccct taattctccg ctcatgatga
     6661 attcagaaat aaattcagcc taattcggcg ttaattcagt acattaaaaa cgtccgcaat
     6721 gtgttattaa gttgtctaag cgtcaatttg tttacaccac aatatatcct gccaccagcc
     6781 agccaacagc tccccgaccg gcagctcggc acaaaatcac cactcgatac aggcagccca
     6841 tcagtccggg acggcgtcag cgggagagcc gttgtaaggc ggcagacttt gctcatgtta
     6901 ccgatgctat tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc
     6961 ggagggtagc atgttgattg taacgatgac agagcgttgc tgcctgtgat caattcgggc
     7021 acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg
     7081 ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt
     7141 tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca
     7201 gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca
     7261 gcagggcagt cgccctaaaa caaagttaaa catcatgggg gaagcggtga tcgccgaagt
     7321 atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct
     7381 ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga
     7441 tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga
     7501 ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac
     7561 cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt
     7621 tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat
     7681 tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc
     7741 ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac
     7801 cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac
     7861 gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc
     7921 cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca
     7981 ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt
     8041 tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc tagctagaaa
     8101 ttcgttcaag ccgacgccgc ttcgcggcgc ggcttaactc aagcgttaga tgcactaagc
     8161 acataattgc tcacagccaa actatcaggt caagtctgct tttattattt ttaagcgtgc
     8221 ataataagcc ctacacaaat tgggagatat atcatgcatg accaaaatcc cttaacgtga
     8281 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     8341 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     8401 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     8461 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     8521 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     8581 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     8641 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     8701 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     8761 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     8821 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     8881 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     8941 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     9001 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     9061 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt
     9121 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg
     9181 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg
     9241 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     9301 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     9361 ccgtcatcac cgaaacgcgc gaggcagggt gccttgatgt gggcgccggc ggtcgagtgg
     9421 cgacggcgcg gcttgtccgc gccctggtag attgcctggc cgtaggccag ccatttttga
     9481 gcggccagcg gccgcgatag gccgacgcga agcggcgggg cgtagggagc gcagcgaccg
     9541 aagggtaggc gctttttgca gctcttcggc tgtgcgctgg ccagacagtt atgcacaggc
     9601 caggcgggtt ttaagagttt taataagttt taaagagttt taggcggaaa aatcgccttt
     9661 tttctctttt atatcagtca cttacatgtg tgaccggttc ccaatgtacg gctttgggtt
     9721 cccaatgtac gggttccggt tcccaatgta cggctttggg ttcccaatgt acgtgctatc
     9781 cacaggaaag agaccttttc gacctttttc ccctgctagg gcaatttgcc ctagcatctg
     9841 ctccgtacat taggaaccgg cggatgcttc gccctcgatc aggttgcggt agcgcatgac
     9901 taggatcggg ccagcctgcc ccgcctcctc cttcaaatcg tactccggca ggtcatttga
     9961 cccgatcagc ttgcgcacgg tgaaacagaa cttcttgaac tctccggcgc tgccactgcg
    10021 ttcgtagatc gtcttgaaca accatctggc ttctgccttg cctgcggcgc ggcgtgccag
    10081 gcggtagaga aaacggccga tgccgggatc gatcaaaaag taatcggggt gaaccgtcag
    10141 cacgtccggg ttcttgcctt ctgtgatctc gcggtacatc caatcagcta gctcgatctc
    10201 gatgtactcc ggccgcccgg tttcgctctt tacgatcttg tagcggctaa tcaaggcttc
    10261 accctcggat accgtcacca ggcggccgtt cttggccttc ttcgtacgct gcatggcaac
    10321 gtgcgtggtg tttaaccgaa tgcaggtttc taccaggtcg tctttctgct ttccgccatc
    10381 ggctcgccgg cagaacttga gtacgtccgc aacgtgtgga cggaacacgc ggccgggctt
    10441 gtctcccttc ccttcccggt atcggttcat ggattcggtt agatgggaaa ccgccatcag
    10501 taccaggtcg taatcccaca cactggccat gccggccggc cctgcggaaa cctctacgtg
    10561 cccgtctgga agctcgtagc ggatcacctc gccagctcgt cggtcacgct tcgacagacg
    10621 gaaaacggcc acgtccatga tgctgcgact atcgcgggtg cccacgtcat agagcatcgg
    10681 aacgaaaaaa tctggttgct cgtcgccctt gggcggcttc ctaatcgacg gcgcaccggc
    10741 tgccggcggt tgccgggatt ctttgcggat tcgatcagcg gccgcttgcc acgattcacc
    10801 ggggcgtgct tctgcctcga tgcgttgccg ctgggcggcc tgcgcggcct tcaacttctc
    10861 caccaggtca tcacccagcg ccgcgccgat ttgtaccggg ccggatggtt tgcgaccgct
    10921 cacgccgatt cctcgggctt gggggttcca gtgccattgc agggccggca gacaacccag
    10981 ccgcttacgc ctggccaacc gcccgttcct ccacacatgg ggcattccac ggcgtcggtg
    11041 cctggttgtt cttgattttc catgccgcct cctttagccg ctaaaattca tctactcatt
    11101 tattcatttg ctcatttact ctggtagctg cgcgatgtat tcagatagca gctcggtaat
    11161 ggtcttgcct tggcgtaccg cgtacatctt cagcttggtg tgatcctccg ccggcaactg
    11221 aaagttgacc cgcttcatgg ctggcgtgtc tgccaggctg gccaacgttg cagccttgct
    11281 gctgcgtgcg ctcggacggc cggcacttag cgtgtttgtg cttttgctca ttttctcttt
    11341 acctcattaa ctcaaatgag ttttgattta atttcagcgg ccagcgcctg gacctcgcgg
    11401 gcagcgtcgc cctcgggttc tgattcaaga acggttgtgc cggcggcggc agtgcctggg
    11461 tagctcacgc gctgcgtgat acgggactca agaatgggca gctcgtaccc ggccagcgcc
    11521 tcggcaacct caccgccgat gcgcgtgcct ttgatcgccc gcgacacgac aaaggccgct
    11581 tgtagccttc catccgtgac ctcaatgcgc tgcttaacca gctccaccag gtcggcggtg
    11641 gcccatatgt cgtaagggct tggctgcacc ggaatcagca cgaagtcggc tgccttgatc
    11701 gcggacacag ccaagtccgc cgcctggggc gctccgtcga tcactacgaa gtcgcgccgg
    11761 ccgatggcct tcacgtcgcg gtcaatcgtc gggcggtcga tgccgacaac ggttagcggt
    11821 tgatcttccc gcacggccgc ccaatcgcgg gcactgccct ggggatcgga atcgactaac
    11881 agaacatcgg ccccggcgag ttgcagggcg cgggctagat gggttgcgat ggtcgtcttg
    11941 cctgacccgc ctttctggtt aagtacagcg ataaccttca tgcgttcccc ttgcgtattt
    12001 gtttatttac tcatcgcatc atatacgcag cgaccgcatg acgcaagctg ttttactcaa
    12061 atacacatca cctttttaga cggcggcgct cggtttcttc agcggccaag ctggccggcc
    12121 aggccgccag cttggcatca gacaaaccgg ccaggatttc atgcagccgc acggttgaga
    12181 cgtgcgcggg cggctcgaac acgtacccgg ccgcgatcat ctccgcctcg atctcttcgg
    12241 taatgaaaaa cggttcgtcc tggccgtcct ggtgcggttt catgcttgtt cctcttggcg
    12301 ttcattctcg gcggccgcca gggcgtcggc ctcggtcaat gcgtcctcac ggaaggcacc
    12361 gcgccgcctg gcctcggtgg gcgtcacttc ctcgctgcgc tcaagtgcgc ggtacagggt
    12421 cgagcgatgc acgccaagca gtgcagccgc ctctttcacg gtgcggcctt cctggtcgat
    12481 cagctcgcgg gcgtgcgcga tctgtgccgg ggtgagggta gggcgggggc caaacttcac
    12541 gcctcgggcc ttggcggcct cgcgcccgct ccgggtgcgg tcgatgatta gggaacgctc
    12601 gaactcggca atgccggcga acacggtcaa caccatgcgg ccggccggcg tggtggtgtc
    12661 ggcccacggc tctgccaggc tacgcaggcc cgcgccggcc tcctggatgc gctcggcaat
    12721 gtccagtagg tcgcgggtgc tgcgggccag gcggtctagc ctggtcactg tcacaacgtc
    12781 gccagggcgt aggtggtcaa gcatcctggc cagctccggg cggtcgcgcc tggtgccggt
    12841 gatcttctcg gaaaacagct tggtgcagcc ggccgcgtgc agttcggccc gttggttggt
    12901 caagtcctgg tcgtcggtgc tgacgcgggc atagcccagc aggccagcgg cggcgctctt
    12961 gttcatggcg taatgtctcc ggttctagtc gcaagtattc tactttatgc gactaaaaca
    13021 cgcgacaaga aaacgccagg aaaagggcag ggcggcagcc tgtcgcgtaa cttaggactt
    13081 gtgcgacatg tcgttttcag aagacggctg cactgaacgt cagaagccga ctgcactata
    13141 gcagcggagg ggttggatca aagtactttg atcccgaggg gaaccctgtg gttggcatgc
    13201 acatacaaat ggacgaacgg ataaaccttt tcacgccctt ttaaatatcc gattattcta
    13261 ataaacgctc ttttctctta ggtttacccg ccaatatatc ctgtcaaaca ctgatagttt
    13321 aaactgaagg cgggaaacga caatctgatc caagctcaag ctgctctagc attcgccatt
    13381 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
    13441 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
    13501 acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcc ccagattagc
    13561 cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta cgcagcaggt
    13621 ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct tcccaagaag
    13681 gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga gaaagatata
    13741 tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct tcacaaacca
    13801 aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc aaaggccatg
    13861 gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg cgaacagttc
    13921 atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat ggtggagcac
    13981 gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca aagggcaat
//
