LOCUS       sequence-310397-       14049 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible dual fluorescent ratiometric binary 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14049)
  AUTHORS   Khosla A, Rodriguez-Furlan C, Kapoor S, Van Norman JM, Nelson DC
  TITLE     A series of dual-reporter vectors for ratiometric analysis of 
            protein abundance in plants.
  JOURNAL   Plant Direct. 2020 Jun 21;4(6):e00231. doi: 10.1002/pld3.231. 
            eCollection 2020 Jun.
  PUBMED    32582876
REFERENCE   2  (bases 1 to 14049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2020-06-21"; volume: "4(6):e00231. doi"; pages: " 
            10.1002/pld3.231. eCollection 2020 Jun"
COMMENT     Sequence Label: pRATIO4212
FEATURES             Location/Qualifiers
     source          1..14049
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1620..1640)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    1650..1774
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1811..1816,1817..1834,1835..1841)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1895..2554
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1961..1980)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2896..3201
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3080..3099
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3242..3366)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4166..4231
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             join(4238..4240,4241..4243,4244..4951)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(4283..4304)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4544..4563)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4891..4912
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      4986..5238
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(5271..5287)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5271..5287)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5284..5306)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5295..5311
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5319..5325,5326..5343,5344..5349))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5364..5385
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(5419..5671)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6061..6855)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6172..6191)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6782..6801
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(6876..7059)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(6912..7091)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    7265..7289
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7810..8601
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8847..9435
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9336..9355
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9589..9606
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9621..9761
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9687..9706)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9847..9869
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10105..10299
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10365..11438)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11867..12496)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    13797..13821
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     14009..14031
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     14023..14040
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14024..14040
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgacgtcgta tgatcgcgaa gcacccaccc taagccacat tttaatcctt ctgttggcca
       61 tgccccatca aagttgcact taacccaaga ttgtggtgga gcttcccatg tttctcgtct
      121 gtcccgacgg tgttgtggtt ggtgctttcc ttacattctg agcctctttc cttctaatcc
      181 actcatctgc atcttcttgt gtccttacta atacctcatt ggttccaaat tccctccctt
      241 taagcaccag ctcgtttctg ttcttccaca gcctcccaag tatccaaggg actaaagcct
      301 ccacattctt cagatcagga tattcttgtt taagatgttg aactctatgg aggtttgtat
      361 gaactgatga tctaggaccg gataagttcc cttcttcata gcgaacttat tcaaagaatg
      421 ttttgtgtat cattcttgtt acattgttat taatgaaaaa atattattgg tcattggact
      481 gaacacgagt gttaaatatg gaccaggccc caaataagat ccattgatat atgaattaaa
      541 taacaagaat aaatcgagtc accaaaccac ttgccttttt taacgagact tgttcaccaa
      601 cttgatacaa aagtcattat cctatgcaaa tcaataatca tacaaaaata tccaataaca
      661 ctaaaaaatt aaaagaaatg gataatttca caatatgtta tacgataaag aagttacttt
      721 tccaagaaat tcactgattt tataagccca cttgcattag ataaatggca aaaaaaaaca
      781 aaaaggaaaa gaaataaagc acgaagaatt ccagaaaata cgaaatacgc ttcaatgcag
      841 tgggacccac ggttcaatta ttgccaattt tcagctccac cgtatattta aaaaataaaa
      901 cgataatgct aaaaaaatat aaatcgtaac gatcgttaaa tctcaacggc tggatcttat
      961 gacgaccgtt agaaattgtg gttgtcgacg agtcagtaat aaacggcgtc aaagtggttg
     1021 cagccggcac acacgagtcg tgtttatcaa ctcaaagcac aaatactttt cctcaaccta
     1081 aaaataaggc aattagccaa aaacaacttt gcgtgtaaac aacgctcaat acacgtgtca
     1141 ttttattatt agctattgct tcaccgcctt agctttctcg tgacctagtc gtcctcgtct
     1201 tttcttcttc ttcttctata aaacaatacc caaagagttc ttcttcttca caattcagat
     1261 ttcaatttct caaaatctta aaaactttct ctcaattctc tctaccgtga tcaaggtaaa
     1321 tttctgtgtt ccttattctc tcaaaatctt cgattttgtt ttcgttcgat cccaatttcg
     1381 tatatgttct ttggtttaga ttctgttaat cttagatcga agacgatttt ctgggtttga
     1441 tcgttagata tcatcttaat tctcgattag ggtttcatag atatcatccg atttgttcaa
     1501 ataatttgag ttttgtcgaa taattactct tcgatttgtg atttctatcc agatctggtg
     1561 ttagtttcta gtttgtgcga tcgaatttgt cctcgagagg ggactctagt ggggtcttct
     1621 acctttctct tcttttttgg cattctagta caagtttgta caaaaaagct gaacgagaaa
     1681 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
     1741 aatactgtaa aacacaacat atccagtcac tatggcggcc cctcgagggg ccgcattagg
     1801 caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga
     1861 tccggcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
     1921 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
     1981 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
     2041 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
     2101 tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
     2161 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
     2221 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
     2281 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
     2341 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
     2401 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
     2461 tcaggttcat catgccgtct gtgatggctt ccatgtcggc agaatgctta atgaattaca
     2521 acagtactgc gatgagtggc agggcggggc gtaaagatct ggatccggct tactaaaagc
     2581 cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat
     2641 gtatacccga agtatgtcaa aaagaggtgt gctatgaagc agcgtattac agtgacagtt
     2701 gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa
     2761 gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc
     2821 aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga
     2881 acagggactg gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt
     2941 ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc
     3001 ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat
     3061 atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt
     3121 atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac
     3181 ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga
     3241 ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat
     3301 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa
     3361 agtggtgggc ccaatggtgt ccaagggcga agctgtgatt aaagaattta tgcgttttaa
     3421 ggtccacatg gaaggatcca tgaatggaca tgaattcgaa atcgaggggg agggcgaggg
     3481 ccgaccttat gagggtacac agaccgcgaa gctaaaggta accaagggag gcccactacc
     3541 ctttagttgg gacatactca gcccgcagtt tatgtatggc agtcgtgcct ttataaagca
     3601 cccagcggat ataccagact actacaaaca gtccttccca gaggggttta aatgggagcg
     3661 agtgatgaac tttgaggacg gtggtgctgt gacggtaact caggacacat cactcgaaga
     3721 cgggactctt atttacaaag taaaactgag agggaccaat tttccaccag atggccccgt
     3781 catgcaaaaa aagacgatgg gttgggaagc atctacagaa cgactctatc ccgaggacgg
     3841 cgtcctcaaa ggcgacatta aaatggcctt gaggttgaaa gacggtggac gttacttggc
     3901 ggacttcaaa acaacttata aagcaaaaaa gccagtgcaa atgcctggag cgtacaatgt
     3961 agatagaaaa cttgatataa cgtcacataa tgaggactat accgtggttg agcaatacga
     4021 gaggtcagaa ggacgacaca gcaccggcgg tatggacgag ttgtacaaga cgcgttctaa
     4081 tgccgccgat gaggttgcga ctggatcagg tcttttagcg atacatccga ccgaagcgcg
     4141 acataaacaa aaaattgtag cgccggtcaa acaaaccctg aatttcgact tactcaaact
     4201 tgcgggtgac gtagagtcaa atccagggcc tggtaccatg gtgagcaagg gcgaggagct
     4261 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     4321 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagctgat
     4381 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg
     4441 cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     4501 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     4561 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     4621 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     4681 ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat
     4741 ccgccacaac atcgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc
     4801 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct
     4861 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     4921 cgggatcact ctcggcatgg acgagctgta cattgctgca gcggcctaag agctcgaatt
     4981 tccccgatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc
     5041 ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt
     5101 aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt
     5161 aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt
     5221 catctatgtt actagatcgg gaattggttc cggaaccaat tcgtaatcat gtcatagctg
     5281 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     5341 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaggctg aattaggcgc
     5401 gcctatttct gaattcccga tctagtaaca tagatgacac cgcgcgcgat aatttatcct
     5461 agtttgcgcg ctatattttg ttttctatcg cgtattaaat gtataattgc gggactctaa
     5521 tcataaaaac ccatctcata aataacgtca tgcattacat gttaattatt acatgcttaa
     5581 cgtaattcaa cagaaattat atgataatca tcgcaagacc ggcaacagga ttcaatctta
     5641 agaaacttta ttgccaaatg tttgaacgat cggggatcat ccgggtctgt ggcgggaact
     5701 ccacgaaaat atccgaacgc agcaagatat cgcggtgcat ctcggtcttg cctgggcagt
     5761 cgccgccgac gccgttgatg tggacgccgg gcccgatcat attgtcgctc aggatcgtgg
     5821 cgttgtgctt gtcggccgtt gctgtcgtaa tgatatcggc accttcgacc gcctgttccg
     5881 cagagatccc gtgggcgaag aactccagca tgagatcccc gcgctggagg atcatccagc
     5941 cggcgtcccg gaaaacgatt ccgaagccca acctttcata gaaggcggcg gtggaatcga
     6001 aatctcgtga tggcaggttg ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc
     6061 tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat
     6121 accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg
     6181 ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa
     6241 tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac
     6301 gacgagatca tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc
     6361 gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt
     6421 acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag
     6481 cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg
     6541 agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc
     6601 agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg
     6661 cgctgcctcg tcctgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac
     6721 cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg
     6781 tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc
     6841 atcttgttca atcatgcgaa acgatccaga tccggtgcag attatttgga ttgagagtga
     6901 atatgagact ctaattggat accgagggga atttatggaa cgtcagtgga gcatttttga
     6961 caagaaatat ttgctagctg atagtgacct taggcgactt ttgaacgcgc aataatggtt
     7021 tctgacgtat gtgcttagct cattaaactc cagaaacccg cggctgagtg gctccttcaa
     7081 cgttgcggtt ctgtcagttc caaacgtaaa acggcttgtc ccgcgtcatc ggcgggggtc
     7141 ataacgtgac tcccttaatt ctccgctcat gatgaattca gaaataaatt cagcctaatt
     7201 cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca
     7261 atttgtttac accacaatat atcctgccac cagccagcca acagctcccc gaccggcagc
     7321 tcggcacaaa atcaccactc gatacaggca gcccatcagt ccgggacggc gtcagcggga
     7381 gagccgttgt aaggcggcag actttgctca tgttaccgat gctattcgga agaacggcaa
     7441 ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt gattgtaacg
     7501 atgacagagc gttgctgcct gtgatcaatt cgggcacgaa cccagtggac ataagcctgt
     7561 tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg
     7621 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt
     7681 tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga
     7741 tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag
     7801 ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt
     7861 ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca
     7921 gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg
     7981 cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct
     8041 ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt
     8101 ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt
     8161 cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa
     8221 gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt
     8281 cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc
     8341 gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca
     8401 gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg
     8461 gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat
     8521 cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc
     8581 accaaggtag tcggcaaata atgtctagct agaaattcgt tcaagccgac gccgcttcgc
     8641 ggcgcggctt aactcaagcg ttagatgcac taagcacata attgctcaca gccaaactat
     8701 caggtcaagt ctgcttttat tatttttaag cgtgcataat aagccctaca caaattggga
     8761 gatatatcat gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     8821 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     8881 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     8941 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     9001 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     9061 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     9121 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     9181 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     9241 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     9301 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     9361 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     9421 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     9481 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     9541 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     9601 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     9661 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     9721 tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac
     9781 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     9841 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc
     9901 agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct
     9961 ggtagattgc ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga
    10021 cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct
    10081 tcggctgtgc gctggccaga cagttatgca caggccaggc gggttttaag agttttaata
    10141 agttttaaag agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac
    10201 atgtgtgacc ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca
    10261 atgtacggct ttgggttccc aatgtacgtg ctatccacag gaaagagacc ttttcgacct
    10321 ttttcccctg ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat
    10381 gcttcgccct cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc
    10441 tcctccttca aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa
    10501 cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat
    10561 ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg
    10621 ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg
    10681 atctcgcggt acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg
    10741 ctctttacga tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg
    10801 ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag
    10861 gtttctacca ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg
    10921 tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg
    10981 ttcatggatt cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg
    11041 gccatgccgg ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc
    11101 acctcgccag ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg
    11161 cgactatcgc gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg
    11221 cccttgggcg gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg
    11281 cggattcgat cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt
    11341 tgccgctggg cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg
    11401 ccgatttgta ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg
    11461 ttccagtgcc attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg
    11521 ttcctccaca catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc
    11581 cgcctccttt agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt
    11641 agctgcgcga tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac
    11701 atcttcagct tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc
    11761 gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca
    11821 cttagcgtgt ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg
    11881 atttaatttc agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt
    11941 caagaacggt tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg
    12001 actcaagaat gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg
    12061 tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa
    12121 tgcgctgctt aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct
    12181 gcaccggaat cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct
    12241 ggggcgctcc gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa
    12301 tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat
    12361 cgcgggcact gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca
    12421 gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta
    12481 cagcgataac cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata
    12541 cgcagcgacc gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg
    12601 gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa
    12661 accggccagg atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta
    12721 cccggccgcg atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc
    12781 gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg
    12841 tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc
    12901 acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca
    12961 gccgcctctt tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt
    13021 gccggggtga gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc
    13081 ccgctccggg tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg
    13141 gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc
    13201 aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg
    13261 gccaggcggt ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc
    13321 ctggccagct ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg
    13381 cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg
    13441 cgggcatagc ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc
    13501 tagtcgcaag tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag
    13561 ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac
    13621 ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta
    13681 ctttgatccc gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa
    13741 ccttttcacg cccttttaaa tatccgatta ttctaataaa cgctcttttc tcttaggttt
    13801 acccgccaat atatcctgtc aaacactgat agtttaaact gaaggcggga aacgacaatc
    13861 tgatccaagc tcaagctgct ctagcattcg ccattcaggc tgcgcaactg ttgggaaggg
    13921 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
    13981 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
    14041 gccaagctt
//
