LOCUS       sequence-31040-e         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  TITLE     The Parkinson Disease-associated Leucine-rich Repeat Kinase 2 
            (LRRK2) Is a Dimer That Undergoes Intramolecular Autophosphorylation
  JOURNAL   J Biol Chem. 2008 Jun 13;283(24):16906-14.
  PUBMED    18397888
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2008-06-13"; volume: "283(24)"; pages: "16906-14"
COMMENT     Sequence Label: 2XMyc-LRRK2-deltaHeat
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        51..69
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      101..110
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             110..139
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             155..184
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    203..227
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gagctggttt agtgaaccgt cagatccgct agcgattacg ccaagctcga aattaaccct
       61 cactaaaggg aacaaaagct ggagctccac cgcggtggcg gccgccatgg agcagaaact
      121 catctctgaa gaggatctga gcccgggcgg atccgagcag aaactcatct ctgaagagga
      181 tctgctgcag gaattcgata tcacaagttt gtacaaaaaa gcaggctccg aattcgccct
      241 tctggatgtg gccaacaata gcatttgcct tggaggattt tgtataggaa aagttgaacc
      301 ttcttggctt ggtcctttat ttccagataa gacttctaat ttaaggaaac aaacaaatat
      361 agcatctaca ctagcaagaa tggtgatcag atatcagatg aaaagtgctg tggaagaagg
      421 aacagcctca ggcagcgatg gaaatttttc tgaagatgtg ctgtctaaat ttgatgaatg
      481 gacctttatt cctgactctt ctatggacag tgtgtttgct caaagtgatg acctggatag
      541 tgaaggaagt gaaggctcat ttcttgtgaa aaagaaatct aattcaatta gtgtaggaga
      601 attttaccga gatgccgtat tacagcgttg ctcaccaaat ttgcaaagac attccaattc
      661 cttggggccc atttttgatc atgaagattt actgaagcga aaaagaaaaa tattatcttc
      721 agatgattca ctcaggtcat caaaacttca atcccatatg aggcattcag acagcatttc
      781 ttctctggct tctgagagag aatatattac atcactagac ctttcagcaa atgaactaag
      841 agatattgat gccctaagcc agaaatgctg tataagtgtt catttggagc atcttgaaaa
      901 gctggagctt caccagaatg cactcacgag ctttccacac
//
