LOCUS       sequence-310402-       14034 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible dual fluorescent ratiometric binary 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14034)
  AUTHORS   Khosla A, Rodriguez-Furlan C, Kapoor S, Van Norman JM, Nelson DC
  TITLE     A series of dual-reporter vectors for ratiometric analysis of 
            protein abundance in plants.
  JOURNAL   Plant Direct. 2020 Jun 21;4(6):e00231. doi: 10.1002/pld3.231. 
            eCollection 2020 Jun.
  PUBMED    32582876
REFERENCE   2  (bases 1 to 14034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2020-06-21"; volume: "4(6):e00231. doi"; pages: " 
            10.1002/pld3.231. eCollection 2020 Jun"
COMMENT     Sequence Label: pRATIO4214
FEATURES             Location/Qualifiers
     source          1..14034
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1611..1735
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1772..1777,1778..1795,1796..1802)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1856..2515
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1922..1941)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2857..3162
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3041..3060
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3203..3327)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4127..4192
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             4922..4942
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      4971..5223
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(5256..5272)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5256..5272)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5269..5291)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5280..5296
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5304..5310,5311..5328,5329..5334))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5349..5370
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(5404..5656)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6046..6840)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6157..6176)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6767..6786
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(6861..7044)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(6897..7076)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    7250..7274
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7795..8586
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8832..9420
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9321..9340
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9574..9591
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9606..9746
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9672..9691)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9832..9854
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10090..10284
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10350..11423)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11852..12481)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    13782..13806
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     13994..14016
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     14008..14025
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14009..14025
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgacgtcgta tgatcgcgaa gcacccaccc taagccacat tttaatcctt ctgttggcca
       61 tgccccatca aagttgcact taacccaaga ttgtggtgga gcttcccatg tttctcgtct
      121 gtcccgacgg tgttgtggtt ggtgctttcc ttacattctg agcctctttc cttctaatcc
      181 actcatctgc atcttcttgt gtccttacta atacctcatt ggttccaaat tccctccctt
      241 taagcaccag ctcgtttctg ttcttccaca gcctcccaag tatccaaggg actaaagcct
      301 ccacattctt cagatcagga tattcttgtt taagatgttg aactctatgg aggtttgtat
      361 gaactgatga tctaggaccg gataagttcc cttcttcata gcgaacttat tcaaagaatg
      421 ttttgtgtat cattcttgtt acattgttat taatgaaaaa atattattgg tcattggact
      481 gaacacgagt gttaaatatg gaccaggccc caaataagat ccattgatat atgaattaaa
      541 taacaagaat aaatcgagtc accaaaccac ttgccttttt taacgagact tgttcaccaa
      601 cttgatacaa aagtcattat cctatgcaaa tcaataatca tacaaaaata tccaataaca
      661 ctaaaaaatt aaaagaaatg gataatttca caatatgtta tacgataaag aagttacttt
      721 tccaagaaat tcactgattt tataagccca cttgcattag ataaatggca aaaaaaaaca
      781 aaaaggaaaa gaaataaagc acgaagaatt ccagaaaata cgaaatacgc ttcaatgcag
      841 tgggacccac ggttcaatta ttgccaattt tcagctccac cgtatattta aaaaataaaa
      901 cgataatgct aaaaaaatat aaatcgtaac gatcgttaaa tctcaacggc tggatcttat
      961 gacgaccgtt agaaattgtg gttgtcgacg agtcagtaat aaacggcgtc aaagtggttg
     1021 cagccggcac acacgagtcg tgtttatcaa ctcaaagcac aaatactttt cctcaaccta
     1081 aaaataaggc aattagccaa aaacaacttt gcgtgtaaac aacgctcaat acacgtgtca
     1141 ttttattatt agctattgct tcaccgcctt agctttctcg tgacctagtc gtcctcgtct
     1201 tttcttcttc ttcttctata aaacaatacc caaagagttc ttcttcttca caattcagat
     1261 ttcaatttct caaaatctta aaaactttct ctcaattctc tctaccgtga tcaaggtaaa
     1321 tttctgtgtt ccttattctc tcaaaatctt cgattttgtt ttcgttcgat cccaatttcg
     1381 tatatgttct ttggtttaga ttctgttaat cttagatcga agacgatttt ctgggtttga
     1441 tcgttagata tcatcttaat tctcgattag ggtttcatag atatcatccg atttgttcaa
     1501 ataatttgag ttttgtcgaa taattactct tcgatttgtg atttctatcc agatctggtg
     1561 ttagtttcta gtttgtgcga tcgaatttgt cctcgagagg ggactctagt acaagtttgt
     1621 acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt
     1681 tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca ctatggcggc
     1741 ccctcgaggg gccgcattag gcaccccagg ctttacactt tatgcttccg gctcgtataa
     1801 tgtgtggatt ttgagttagg atccggcgag attttcagga gctaaggaag ctaaaatgga
     1861 gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt
     1921 tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac
     1981 ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat
     2041 tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct
     2101 ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt
     2161 ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca
     2221 agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat
     2281 gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa
     2341 tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa
     2401 ggtgctgatg ccgctggcga ttcaggttca tcatgccgtc tgtgatggct tccatgtcgg
     2461 cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaaagatc
     2521 tggatccggc ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg
     2581 tataagaata tatactgata tgtatacccg aagtatgtca aaaagaggtg tgctatgaag
     2641 cagcgtatta cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg
     2701 tcaatatctc cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg
     2761 aacgctggaa agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga
     2821 acggctcttt tgctgacgag aacagggact ggtgaaatgc agtttaaggt ttacacctat
     2881 aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc
     2941 gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt
     3001 gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg
     3061 gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat
     3121 gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata
     3181 cacagccagt ctgcaggtcg accatagtga ctggatatgt tgtgttttac agtattatgt
     3241 agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc
     3301 tcgttcagct ttcttgtaca aagtggtggg cccaatggtg tccaagggcg aagctgtgat
     3361 taaagaattt atgcgtttta aggtccacat ggaaggatcc atgaatggac atgaattcga
     3421 aatcgagggg gagggcgagg gccgacctta tgagggtaca cagaccgcga agctaaaggt
     3481 aaccaaggga ggcccactac cctttagttg ggacatactc agcccgcagt ttatgtatgg
     3541 cagtcgtgcc tttataaagc acccagcgga tataccagac tactacaaac agtccttccc
     3601 agaggggttt aaatgggagc gagtgatgaa ctttgaggac ggtggtgctg tgacggtaac
     3661 tcaggacaca tcactcgaag acgggactct tatttacaaa gtaaaactga gagggaccaa
     3721 ttttccacca gatggccccg tcatgcaaaa aaagacgatg ggttgggaag catctacaga
     3781 acgactctat cccgaggacg gcgtcctcaa aggcgacatt aaaatggcct tgaggttgaa
     3841 agacggtgga cgttacttgg cggacttcaa aacaacttat aaagcaaaaa agccagtgca
     3901 aatgcctgga gcgtacaatg tagatagaaa acttgatata acgtcacata atgaggacta
     3961 taccgtggtt gagcaatacg agaggtcaga aggacgacac agcaccggcg gtatggacga
     4021 gttgtacaag acgcgttcta atgccgccga tgaggttgcg actggatcag gtcttttagc
     4081 gatacatccg accgaagcgc gacataaaca aaaaattgta gcgccggtca aacaaaccct
     4141 gaatttcgac ttactcaaac ttgcgggtga cgtagagtca aatccagggc ctggtaccat
     4201 ggtatcaaag ggcgaggaat tgtttacggg cgttgtacca atcctggtcg aactggacgg
     4261 cgacgttaac ggacataaat tttcagtaag cggggaaggt gagggggatg caacgtacgg
     4321 gaagctgact ttaaaattta tatgcaccac agggaagcta cctgtgccct ggcctacgtt
     4381 ggtaacgacc ctgacatggg gtgtacagtg ctttgcaaga tacccagacc acatgaagca
     4441 gcacgatttc tttaagtctg ccatgcctga gggttatgta caagagcgta caattttttt
     4501 taaagatgat ggcaactata agacccgtgc cgaggtaaaa tttgaggggg atactctagt
     4561 gaatagaatc gaattgaagg gtatagattt caaggaggac ggaaacatct tggggcataa
     4621 actagagtac aacgccatca gtgacaacgt atatattaca gcggacaaac aaaaaaacgg
     4681 cataaaagca aactttaaga ttcgtcacaa catcgaggac ggcagtgtgc agctggcaga
     4741 ccactaccag caaaatacac caataggaga tggacctgta ttactgccag acaaccacta
     4801 cttatccact cagagtaaat taagtaagga ccctaacgag aaaagggatc acatggtcct
     4861 actagagttc gtgacggcag ccggaattac tttaggcatg gacgaactgt acaaggaatt
     4921 cccaaaaaag aagagaaagg tagaagaccc ctaagagctc gaatttcccc gatcgttcaa
     4981 acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca
     5041 tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat
     5101 ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa
     5161 acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag
     5221 atcgggaatt ggttccggaa ccaattcgta atcatgtcat agctgtttcc tgtgtgaaat
     5281 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     5341 ggtgcctaat gagtgagcta actcacatta ggctgaatta ggcgcgccta tttctgaatt
     5401 cccgatctag taacatagat gacaccgcgc gcgataattt atcctagttt gcgcgctata
     5461 ttttgttttc tatcgcgtat taaatgtata attgcgggac tctaatcata aaaacccatc
     5521 tcataaataa cgtcatgcat tacatgttaa ttattacatg cttaacgtaa ttcaacagaa
     5581 attatatgat aatcatcgca agaccggcaa caggattcaa tcttaagaaa ctttattgcc
     5641 aaatgtttga acgatcgggg atcatccggg tctgtggcgg gaactccacg aaaatatccg
     5701 aacgcagcaa gatatcgcgg tgcatctcgg tcttgcctgg gcagtcgccg ccgacgccgt
     5761 tgatgtggac gccgggcccg atcatattgt cgctcaggat cgtggcgttg tgcttgtcgg
     5821 ccgttgctgt cgtaatgata tcggcacctt cgaccgcctg ttccgcagag atcccgtggg
     5881 cgaagaactc cagcatgaga tccccgcgct ggaggatcat ccagccggcg tcccggaaaa
     5941 cgattccgaa gcccaacctt tcatagaagg cggcggtgga atcgaaatct cgtgatggca
     6001 ggttgggcgt cgcttggtcg gtcatttcga accccagagt cccgctcaga agaactcgtc
     6061 aagaaggcga tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag
     6121 gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat
     6181 gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc
     6241 attttccacc atgatattcg gcaagcaggc atcgccatgg gtcacgacga gatcatcgcc
     6301 gtcgggcatg cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc
     6361 ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat
     6421 gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg
     6481 cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg acaggagatc
     6541 ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag
     6601 cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg cctcgtcctg
     6661 cagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc
     6721 tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc
     6781 gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt gttcaatcat
     6841 gcgaaacgat ccagatccgg tgcagattat ttggattgag agtgaatatg agactctaat
     6901 tggataccga ggggaattta tggaacgtca gtggagcatt tttgacaaga aatatttgct
     6961 agctgatagt gaccttaggc gacttttgaa cgcgcaataa tggtttctga cgtatgtgct
     7021 tagctcatta aactccagaa acccgcggct gagtggctcc ttcaacgttg cggttctgtc
     7081 agttccaaac gtaaaacggc ttgtcccgcg tcatcggcgg gggtcataac gtgactccct
     7141 taattctccg ctcatgatga attcagaaat aaattcagcc taattcggcg ttaattcagt
     7201 acattaaaaa cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac
     7261 aatatatcct gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac
     7321 cactcgatac aggcagccca tcagtccggg acggcgtcag cgggagagcc gttgtaaggc
     7381 ggcagacttt gctcatgtta ccgatgctat tcggaagaac ggcaactaag ctgccgggtt
     7441 tgaaacacgg atgatctcgc ggagggtagc atgttgattg taacgatgac agagcgttgc
     7501 tgcctgtgat caattcgggc acgaacccag tggacataag cctgttcggt tcgtaagctg
     7561 taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt
     7621 ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct
     7681 atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg
     7741 agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgggg
     7801 gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc
     7861 catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg
     7921 aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg
     7981 cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt
     8041 ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca
     8101 gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc
     8161 gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc
     8221 gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta
     8281 tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat
     8341 gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc
     8401 gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc
     8461 gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc
     8521 gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc
     8581 aaataatgtc tagctagaaa ttcgttcaag ccgacgccgc ttcgcggcgc ggcttaactc
     8641 aagcgttaga tgcactaagc acataattgc tcacagccaa actatcaggt caagtctgct
     8701 tttattattt ttaagcgtgc ataataagcc ctacacaaat tgggagatat atcatgcatg
     8761 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     8821 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     8881 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     8941 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     9001 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     9061 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     9121 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     9181 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     9241 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     9301 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     9361 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     9421 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     9481 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     9541 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     9601 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
     9661 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
     9721 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
     9781 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     9841 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagggt gccttgatgt
     9901 gggcgccggc ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag attgcctggc
     9961 cgtaggccag ccatttttga gcggccagcg gccgcgatag gccgacgcga agcggcgggg
    10021 cgtagggagc gcagcgaccg aagggtaggc gctttttgca gctcttcggc tgtgcgctgg
    10081 ccagacagtt atgcacaggc caggcgggtt ttaagagttt taataagttt taaagagttt
    10141 taggcggaaa aatcgccttt tttctctttt atatcagtca cttacatgtg tgaccggttc
    10201 ccaatgtacg gctttgggtt cccaatgtac gggttccggt tcccaatgta cggctttggg
    10261 ttcccaatgt acgtgctatc cacaggaaag agaccttttc gacctttttc ccctgctagg
    10321 gcaatttgcc ctagcatctg ctccgtacat taggaaccgg cggatgcttc gccctcgatc
    10381 aggttgcggt agcgcatgac taggatcggg ccagcctgcc ccgcctcctc cttcaaatcg
    10441 tactccggca ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa cttcttgaac
    10501 tctccggcgc tgccactgcg ttcgtagatc gtcttgaaca accatctggc ttctgccttg
    10561 cctgcggcgc ggcgtgccag gcggtagaga aaacggccga tgccgggatc gatcaaaaag
    10621 taatcggggt gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc gcggtacatc
    10681 caatcagcta gctcgatctc gatgtactcc ggccgcccgg tttcgctctt tacgatcttg
    10741 tagcggctaa tcaaggcttc accctcggat accgtcacca ggcggccgtt cttggccttc
    10801 ttcgtacgct gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc taccaggtcg
    10861 tctttctgct ttccgccatc ggctcgccgg cagaacttga gtacgtccgc aacgtgtgga
    10921 cggaacacgc ggccgggctt gtctcccttc ccttcccggt atcggttcat ggattcggtt
    10981 agatgggaaa ccgccatcag taccaggtcg taatcccaca cactggccat gccggccggc
    11041 cctgcggaaa cctctacgtg cccgtctgga agctcgtagc ggatcacctc gccagctcgt
    11101 cggtcacgct tcgacagacg gaaaacggcc acgtccatga tgctgcgact atcgcgggtg
    11161 cccacgtcat agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt gggcggcttc
    11221 ctaatcgacg gcgcaccggc tgccggcggt tgccgggatt ctttgcggat tcgatcagcg
    11281 gccgcttgcc acgattcacc ggggcgtgct tctgcctcga tgcgttgccg ctgggcggcc
    11341 tgcgcggcct tcaacttctc caccaggtca tcacccagcg ccgcgccgat ttgtaccggg
    11401 ccggatggtt tgcgaccgct cacgccgatt cctcgggctt gggggttcca gtgccattgc
    11461 agggccggca gacaacccag ccgcttacgc ctggccaacc gcccgttcct ccacacatgg
    11521 ggcattccac ggcgtcggtg cctggttgtt cttgattttc catgccgcct cctttagccg
    11581 ctaaaattca tctactcatt tattcatttg ctcatttact ctggtagctg cgcgatgtat
    11641 tcagatagca gctcggtaat ggtcttgcct tggcgtaccg cgtacatctt cagcttggtg
    11701 tgatcctccg ccggcaactg aaagttgacc cgcttcatgg ctggcgtgtc tgccaggctg
    11761 gccaacgttg cagccttgct gctgcgtgcg ctcggacggc cggcacttag cgtgtttgtg
    11821 cttttgctca ttttctcttt acctcattaa ctcaaatgag ttttgattta atttcagcgg
    11881 ccagcgcctg gacctcgcgg gcagcgtcgc cctcgggttc tgattcaaga acggttgtgc
    11941 cggcggcggc agtgcctggg tagctcacgc gctgcgtgat acgggactca agaatgggca
    12001 gctcgtaccc ggccagcgcc tcggcaacct caccgccgat gcgcgtgcct ttgatcgccc
    12061 gcgacacgac aaaggccgct tgtagccttc catccgtgac ctcaatgcgc tgcttaacca
    12121 gctccaccag gtcggcggtg gcccatatgt cgtaagggct tggctgcacc ggaatcagca
    12181 cgaagtcggc tgccttgatc gcggacacag ccaagtccgc cgcctggggc gctccgtcga
    12241 tcactacgaa gtcgcgccgg ccgatggcct tcacgtcgcg gtcaatcgtc gggcggtcga
    12301 tgccgacaac ggttagcggt tgatcttccc gcacggccgc ccaatcgcgg gcactgccct
    12361 ggggatcgga atcgactaac agaacatcgg ccccggcgag ttgcagggcg cgggctagat
    12421 gggttgcgat ggtcgtcttg cctgacccgc ctttctggtt aagtacagcg ataaccttca
    12481 tgcgttcccc ttgcgtattt gtttatttac tcatcgcatc atatacgcag cgaccgcatg
    12541 acgcaagctg ttttactcaa atacacatca cctttttaga cggcggcgct cggtttcttc
    12601 agcggccaag ctggccggcc aggccgccag cttggcatca gacaaaccgg ccaggatttc
    12661 atgcagccgc acggttgaga cgtgcgcggg cggctcgaac acgtacccgg ccgcgatcat
    12721 ctccgcctcg atctcttcgg taatgaaaaa cggttcgtcc tggccgtcct ggtgcggttt
    12781 catgcttgtt cctcttggcg ttcattctcg gcggccgcca gggcgtcggc ctcggtcaat
    12841 gcgtcctcac ggaaggcacc gcgccgcctg gcctcggtgg gcgtcacttc ctcgctgcgc
    12901 tcaagtgcgc ggtacagggt cgagcgatgc acgccaagca gtgcagccgc ctctttcacg
    12961 gtgcggcctt cctggtcgat cagctcgcgg gcgtgcgcga tctgtgccgg ggtgagggta
    13021 gggcgggggc caaacttcac gcctcgggcc ttggcggcct cgcgcccgct ccgggtgcgg
    13081 tcgatgatta gggaacgctc gaactcggca atgccggcga acacggtcaa caccatgcgg
    13141 ccggccggcg tggtggtgtc ggcccacggc tctgccaggc tacgcaggcc cgcgccggcc
    13201 tcctggatgc gctcggcaat gtccagtagg tcgcgggtgc tgcgggccag gcggtctagc
    13261 ctggtcactg tcacaacgtc gccagggcgt aggtggtcaa gcatcctggc cagctccggg
    13321 cggtcgcgcc tggtgccggt gatcttctcg gaaaacagct tggtgcagcc ggccgcgtgc
    13381 agttcggccc gttggttggt caagtcctgg tcgtcggtgc tgacgcgggc atagcccagc
    13441 aggccagcgg cggcgctctt gttcatggcg taatgtctcc ggttctagtc gcaagtattc
    13501 tactttatgc gactaaaaca cgcgacaaga aaacgccagg aaaagggcag ggcggcagcc
    13561 tgtcgcgtaa cttaggactt gtgcgacatg tcgttttcag aagacggctg cactgaacgt
    13621 cagaagccga ctgcactata gcagcggagg ggttggatca aagtactttg atcccgaggg
    13681 gaaccctgtg gttggcatgc acatacaaat ggacgaacgg ataaaccttt tcacgccctt
    13741 ttaaatatcc gattattcta ataaacgctc ttttctctta ggtttacccg ccaatatatc
    13801 ctgtcaaaca ctgatagttt aaactgaagg cgggaaacga caatctgatc caagctcaag
    13861 ctgctctagc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
    13921 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
    13981 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctt
//
