LOCUS       sequence-310406-       11119 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N-terminal FKBP12(F36V)-2xHA tag on lentiviral OGT construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11119)
  AUTHORS   Levine ZG, Potter SC, Joiner CM, Fei GQ, Nabet B, Sonnett M, Zachara
            NE, Gray NS, Paulo JA, Walker S
  TITLE     Mammalian cell proliferation requires noncatalytic functions of 
            O-GlcNAc transferase.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Jan 26;118(4). pii: 2016778118. doi: 
            10.1073/pnas.2016778118.
  PUBMED    33419956
REFERENCE   2  (bases 1 to 11119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-01-26"; volume: "118(4). pii: 
            2016778118. doi"; pages: " 10.1073/pnas.2016778118"
COMMENT     Sequence Label: pLEX_305-N-dTAG_OGT
FEATURES             Location/Qualifiers
     source          1..11119
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..501
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     420..438
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             join(510..512,513..833)
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             840..866
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             840..866
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             870..896
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    912..936
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4061..4085)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4137..4725
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4190..4210)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4608..4619)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4797..5030
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5108..5229
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5145..5164)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5199..5218
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5269..5404
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5424..5443)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5425..5443)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5453..5470)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5453..5469)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5462..5484)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5611..6066
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5698..5717)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5908..5929
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6092..6196
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6197..6265,6266..7057)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6415..6434)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7228..7816
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7717..7736
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7970..7987
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8104..8125
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8140..8145,8146..8163,8164..8170)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8178..8194
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8183..8205
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8202..8218
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8202..8218
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8237..8257
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8239..8257
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8285..8511
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8512..8692
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8739..8864
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9357..9590
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9775..9819
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10050..10260
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        10072..10268
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10119..10254
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10300..10902
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10300..10319)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10796..10816
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10945..11062
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt
       61 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc
      121 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc
      181 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca
      241 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg
      301 cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa
      361 ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg
      421 tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga
      481 atcaccgacc tctctcccca ggggctagca tgggagtgca ggtggaaacc atctccccag
      541 gagacgggcg caccttcccc aagcgcggcc agacctgcgt ggtgcactac accgggatgc
      601 ttgaagatgg aaagaaagtt gattcctccc gggacagaaa caagcccttt aagtttatgc
      661 taggcaagca ggaggtgatc cgaggctggg aagaaggggt tgcccagatg agtgtgggtc
      721 agagagccaa actgactata tctccagatt atgcctatgg tgccactggg cacccaggca
      781 tcatcccacc acatgccact ctcgtcttcg atgtggagct tctaaaactg gaaggcggct
      841 acccctacga cgtgcccgac tacgccggct atccgtatga tgtcccggac tatgcaggca
      901 tcgatagatc aacaagtttg tacaaaaaag caggctttgc gtcttccgtg ggcaacgtgg
      961 ccgacagcac agggttagct gagttggcac atcgagaata tcaggcagga gattttgagg
     1021 cagctgagag acactgcatg cagctctgga gacaagagcc agacaatact ggtgtgcttt
     1081 tattactttc atctatacac ttccagtgtc gaaggctgga cagatctgct cactttagca
     1141 ctctggcaat taaacagaac ccccttctgg cagaagctta ttcgaatttg gggaatgtgt
     1201 acaaggaaag agggcagttg caggaggcaa ttgagcatta tcgacatgca ttgcgtctca
     1261 aacctgattt catcgatggt tatattaacc tggcagccgc cttggtagca gcgggtgaca
     1321 tggaaggggc agtacaagct tacgtctctg ctcttcagta caatcctgat ttgtactgtg
     1381 ttcgcagtga cctggggaac ctgctcaaag ccctgggtcg cttggaagaa gccaaggcat
     1441 gttatttgaa agcaattgag acgcaaccga actttgcagt agcttggagt aatcttggct
     1501 gtgttttcaa tgcacaaggg gaaatttggc ttgcaattca tcactttgaa aaggctgtca
     1561 cccttgaccc aaactttctg gatgcttata tcaatttagg aaatgtcttg aaagaggcac
     1621 gcatttttga cagagctgtg gcagcttatc ttcgtgccct aagtttgagt ccaaatcacg
     1681 cagtggtgca cggcaacctg gcttgtgtat actatgagca aggcctgata gatctggcaa
     1741 tagacaccta caggcgggct atcgaactac aaccacattt ccctgatgct tactgcaacc
     1801 tagccaatgc tctcaaagag aagggcagtg ttgctgaagc agaagattgt tataatacag
     1861 ctctccgtct gtgtcccacc catgcagact ctctgaataa cctagccaat atcaaacgag
     1921 aacagggaaa cattgaagag gcagttcgct tgtatcgtaa agcattagaa gtcttcccag
     1981 agtttgctgc tgcccattca aatttagcaa gtgtactgca gcagcaggga aaactgcagg
     2041 aagctctgat gcattataag gaggctattc gaatcagtcc tacctttgct gatgcctact
     2101 ctaatatggg aaacactcta aaggagatgc aggatgttca gggagccttg cagtgttata
     2161 cgcgtgccat ccaaattaat cctgcatttg cagatgcaca tagcaatctg gcttccattc
     2221 ataaggattc agggaatatt ccagaagcca tagcttctta ccgcacggct ctgaaactta
     2281 agcctgattt tcctgatgct tattgtaact tggctcattg cctgcagatt gtctgtgatt
     2341 ggacagacta tgatgagcga atgaagaagt tggtcagtat tgtggctgac cagttagaga
     2401 agaataggtt gccttctgtg catcctcatc atagtatgct atatcctctt tctcatggct
     2461 tcaggaaggc tattgctgag aggcacggca acctgtgctt agataagatt aatgttcttc
     2521 ataaaccacc atatgaacat ccaaaagact tgaagctcag tgatggtcgg ctgcgtgtag
     2581 gatatgtgag ttccgacttt gggaatcatc ctacttctca ccttatgcag tctattccag
     2641 gcatgcacaa tcctgataaa tttgaggtgt tctgttatgc cctgagccca gacgatggca
     2701 caaacttccg agtgaaggtg atggcagaag ccaatcattt cattgatctt tctcagattc
     2761 catgcaatgg aaaagcagct gatcgcatcc atcaggatgg aattcatatc cttgtaaata
     2821 tgaatggcta tactaagggc gctcgaaatg agctttttgc tctcaggcca gctcctattc
     2881 aggcaatgtg gctgggatac cctgggacga gtggtgcgct tttcatggat tatattatca
     2941 ctgatcagga aacttcgcca gctgaagttg ctgagcagta ttccgagaaa ttggcttata
     3001 tgccccacac tttttttatt ggtgatcatg ctaatatgtt ccctcacctg aagaaaaaag
     3061 cagtcatcga ttttaagtcc aatgggcaca tttatgacaa tcggatagtt ctgaatggca
     3121 tcgacctcaa agcatttctt gatagtctac cagatgtgaa aattgtcaag atgaagtgtc
     3181 ctgatggagg agacaatgca gatagcagta acacagctct taatatgcct gttattccta
     3241 tgaatactat tgcagaagca gttattgaaa tgattaaccg aggacagatt caaataacaa
     3301 ttaatggatt cagtattagc aatggactgg caactactca gatcaacaat aaggctgcaa
     3361 ctggagagga ggttccccgt accattattg taaccacccg ttctcagtac gggttaccag
     3421 aagatgccat cgtatactgt aactttaatc agttgtataa aattgaccct tctactttgc
     3481 agatgtgggc aaacattctg aagcgtgttc ccaatagtgt actctggctg ttgcgttttc
     3541 cagcagtagg agaacctaat attcaacagt atgcacaaaa catgggcctg ccccagaacc
     3601 gtatcatttt ttcacctgtt gctcctaaag aggaacacgt caggagaggc cagctggctg
     3661 atgtctgctt ggacactcca ctctgtaatg ggcacaccac agggatggat gtcctctggg
     3721 cagggacccc catggtgact atgccaggag agactcttgc ttctcgagtt gcagcatccc
     3781 agctcacttg cttaggttgt cttgagctta ttgctaaaaa cagacaagaa tatgaagaca
     3841 tagctgtgaa gctgggaact gatctagaat acctgaagaa agttcgtggc aaagtctgga
     3901 agcaaagaat atctagccct ctgttcaaca ccaaacaata cacaatggaa ctagagcggc
     3961 tctatctaca gatgtgggag cattatgcag ctggcaacaa acctgaccac atgattaagc
     4021 ctgttgaagt cactgagtca gcataactcg agatatctag acccagcttt cttgtacaaa
     4081 gtggttaccg gttagtaatg aactagttaa gtcgacaatc aacgcgttaa gtcgacaatc
     4141 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     4201 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     4261 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     4321 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     4381 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     4441 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     4501 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     4561 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     4621 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     4681 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga ctttaagacc
     4741 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     4801 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     4861 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     4921 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     4981 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtacgtatag
     5041 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     5101 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     5161 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     5221 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     5281 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     5341 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     5401 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     5461 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     5521 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     5581 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     5641 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     5701 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     5761 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     5821 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     5881 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     5941 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     6001 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     6061 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     6121 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     6181 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     6241 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     6301 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     6361 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     6421 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     6481 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     6541 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     6601 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     6661 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     6721 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     6781 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     6841 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     6901 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     6961 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     7021 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     7081 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     7141 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     7201 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     7261 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     7321 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     7381 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     7441 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     7501 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     7561 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     7621 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     7681 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     7741 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     7801 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     7861 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     7921 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     7981 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     8041 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     8101 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     8161 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     8221 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
     8281 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
     8341 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
     8401 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
     8461 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
     8521 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     8581 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     8641 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     8701 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     8761 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     8821 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     8881 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     8941 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     9001 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     9061 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     9121 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     9181 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     9241 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     9301 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     9361 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     9421 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     9481 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     9541 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     9601 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     9661 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     9721 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     9781 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     9841 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     9901 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     9961 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
    10021 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
    10081 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
    10141 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
    10201 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
    10261 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
    10321 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
    10381 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
    10441 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
    10501 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
    10561 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
    10621 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
    10681 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
    10741 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
    10801 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
    10861 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
    10921 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
    10981 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
    11041 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
    11101 ccggctcgag gcctgcagg
//
