LOCUS       sequence-31041-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  TITLE     The Parkinson Disease-associated Leucine-rich Repeat Kinase 2 
            (LRRK2) Is a Dimer That Undergoes Intramolecular Autophosphorylation
  JOURNAL   J Biol Chem. 2008 Jun 13;283(24):16906-14.
  PUBMED    18397888
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2008-06-13"; volume: "283(24)"; pages: "16906-14"
COMMENT     Sequence Label: 2XMyc-LRRK2-COR
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        52..70
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      102..111
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             111..140
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             156..185
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    204..228
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcgagctggt tagtgaaccg tcagatccgc tagcgattac gccaagctcg aaattaaccc
       61 tcactaaagg gaacaaaagc tggagctcca ccgcggtggc ggccgccatg gagcagaaac
      121 tcatctctga agaggatctg agcccgggcg gatccgagca gaaactcatc tctgaagagg
      181 atctgctgca ggaattcgat atcacaagtt tgtacaaaaa agcaggctcc gaattcgccc
      241 ttatgacgca gcgagcattg taccttgctg tctatgacct cagcaaggga caggctgaag
      301 ttgatgccat gaagccttgg ctcttcaata taaaggctcg cgcttcttct tcccctgtga
      361 ttctcgttgg cacacatttg gatgtttctg atgagaagca acgcaaagcc tgcatgagta
      421 aaatcaccaa ggaactcctg aataagcgag ggttccctgc catacgagat taccactttg
      481 tgaatgccac cgaggaatct gatgctttgg caaaacttcg gaaaaccatc ataaacgaga
      541 gccttaattt caagatccga gatcagcttg ttgttggaca gctgattcca gactgctatg
      601 tagaacttga aaaaatcatt ttatcggagc gtaaaaatgt gccaattgaa tttcccgtaa
      661 ttgaccggaa acgattatta caactagtga gagaaaatca gctgcagtta gatgaaaatg
      721 agcttcctca cgcagttcac tttctaaatg aatcaggagt ccttcttcat tttcaagacc
      781 cagcactgca gttaagtgac ttgtactttg tggaacccaa gtggctttgt aaaatcatgg
      841 cacagatttt gacagtgaaa gtggaaggtt gtccaaaaca ccctaaggga attatttcgc
      901 gtagagatgt ggaaaaattt ctttcaaaaa aaaggaaatt tcc
//
