LOCUS       sequence-310412-        1432 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Drives expression of Cas9 and a TdTomato fluorescent marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Gamez S, Chaverra-Rodriguez D, Buchman A, Kandul NP, Mendez-Sanchez 
            SC, Bennett JB, Sanchez C HM, Yang T, Antoshechkin I, Duque JE, 
            Papathanos PA, Marshall JM, Akbari OS
  TITLE     Exploiting a Y chromosome-linked Cas9 for sex selection and gene 
            drive.
  JOURNAL   Nat Commun. 2021 Dec 10;12(1):7202. doi: 10.1038/s41467-021-27333-1.
  PUBMED    34893590
REFERENCE   2  (bases 1 to 1432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-12-10"; volume: "12(1):7202. doi"; pages: " 
            10.1038/s41467-021-27333-1"
COMMENT     Sequence Label: SGyA
FEATURES             Location/Qualifiers
     source          1..1432
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(134..152)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     402..421
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     596..619
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 atggtgagca agggcgagga ggtcatcaaa gagttcatgc gcttcaaggt gcgcatggag
       61 ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
      121 ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac
      181 atcctgtccc cccagttcat gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc
      241 cccgattaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
      301 gaggacggcg gtctggtgac cgtgacccag gactcctccc tgcaggacgg cacgctgatc
      361 tacaaggtga agatgcgcgg caccaacttc ccccccgacg gccccgtaat gcagaagaag
      421 accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc
      481 gagatccacc aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagacc
      541 atctacatgg ccaagaagcc cgtgcaactg cccggctact actacgtgga caccaagctg
      601 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg ctccgagggc
      661 cgccaccacc tgttcctggg gcatggcacc ggcagcaccg gcagcggcag ctccggcacc
      721 gcctcctccg aggacaacaa catggccgtt atcaaggaat ttatgcgctt caaagttagg
      781 atggagggat ccatgaacgg acatgagttc gagatcgagg gagagggcga gggacgcccg
      841 tatgaaggca cacaaacagc caaactcaag gtcaccaagg gcggaccact gcccttcgcc
      901 tgggatatcc tgagtcccca gtttatgtac ggcagcaagg cctacgttaa gcaccccgct
      961 gacataccgg actacaaaaa gctgtccttt ccggaaggct tcaagtggga gcgcgtgatg
     1021 aatttcgaag acggaggact ggtcactgtg acccaagata gcagtttgca ggacggtaca
     1081 ctgatctata aggttaaaat gcgcggcact aactttccgc cagatggccc agtgatgcag
     1141 aagaagacca tgggttggga ggcatccacc gaacgtctgt accctcgaga cggagtgctc
     1201 aagggcgaga tccatcaggc cctcaaactg aaagatggtg gtcactacct ggtcgaattt
     1261 aagaccattt acatggccaa gaagccggtt cagctgcccg gatattatta tgtggatacg
     1321 aaactggata taacttcgca taacgaagac tacaccattg tcgagcagta tgagcgcagc
     1381 gaaggccgac atcacctgtt cctctacggc atggacgagc tgtacaagta gg
//
