LOCUS       sequence-310414-         432 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Drives expression of Cas9 and a TdTomato fluorescent marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Gamez S, Chaverra-Rodriguez D, Buchman A, Kandul NP, Mendez-Sanchez 
            SC, Bennett JB, Sanchez C HM, Yang T, Antoshechkin I, Duque JE, 
            Papathanos PA, Marshall JM, Akbari OS
  TITLE     Exploiting a Y chromosome-linked Cas9 for sex selection and gene 
            drive.
  JOURNAL   Nat Commun. 2021 Dec 10;12(1):7202. doi: 10.1038/s41467-021-27333-1.
  PUBMED    34893590
REFERENCE   2  (bases 1 to 432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-12-10"; volume: "12(1):7202. doi"; pages: " 
            10.1038/s41467-021-27333-1"
COMMENT     Sequence Label: SGyA
FEATURES             Location/Qualifiers
     source          1..432
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    2..432
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
ORIGIN
        1 ttggccacgt aataagtgtg cgttgaattt attcgcaaaa acattgcata ttttcggcaa
       61 agtaaaattt tgttgcatac cttatcaaaa aataagtgct gcatactttt tagagaaacc
      121 aaataatttt ttattgcata cccgttttta ataaaataca ttgcataccc tcttttaata
      181 aaaaatattg catactttga cgaaacaaat tttcgttgca tacccaataa aagattatta
      241 tattgcatac ccgtttttaa taaaatacat tgcataccct cttttaataa aaaatattgc
      301 atacgttgac gaaacaaatt ttcgttgcat acccaataaa agattattat attgcatacc
      361 ttttcttgcc ataccattta gccgatcaat tgtgctcggc aacagtatat ttgtggtgtg
      421 ccaaccaaca ac
//
