LOCUS       sequence-310417-        5353 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector encoding non-tagged O-GlcNAc Transferase with open 
            N-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5353)
  AUTHORS   Levine ZG, Potter SC, Joiner CM, Fei GQ, Nabet B, Sonnett M, Zachara
            NE, Gray NS, Paulo JA, Walker S
  TITLE     Mammalian cell proliferation requires noncatalytic functions of 
            O-GlcNAc transferase.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Jan 26;118(4). pii: 2016778118. doi: 
            10.1073/pnas.2016778118.
  PUBMED    33419956
REFERENCE   2  (bases 1 to 5353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-01-26"; volume: "118(4). pii: 
            2016778118. doi"; pages: " 10.1073/pnas.2016778118"
COMMENT     Sequence Label: pENTR1A hu_ncOGT WT (open N-term)
FEATURES             Location/Qualifiers
     source          1..5353
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3582..3681)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3793..3812)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3804..4613
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3871..3890)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4703..5291
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5192..5211
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggctttg cgtcttccgt gggcaacgtg
      481 gccgacagca cagggttagc tgagttggca catcgagaat atcaggcagg agattttgag
      541 gcagctgaga gacactgcat gcagctctgg agacaagagc cagacaatac tggtgtgctt
      601 ttattacttt catctataca cttccagtgt cgaaggctgg acagatctgc tcactttagc
      661 actctggcaa ttaaacagaa cccccttctg gcagaagctt attcgaattt ggggaatgtg
      721 tacaaggaaa gagggcagtt gcaggaggca attgagcatt atcgacatgc attgcgtctc
      781 aaacctgatt tcatcgatgg ttatattaac ctggcagccg ccttggtagc agcgggtgac
      841 atggaagggg cagtacaagc ttacgtctct gctcttcagt acaatcctga tttgtactgt
      901 gttcgcagtg acctggggaa cctgctcaaa gccctgggtc gcttggaaga agccaaggca
      961 tgttatttga aagcaattga gacgcaaccg aactttgcag tagcttggag taatcttggc
     1021 tgtgttttca atgcacaagg ggaaatttgg cttgcaattc atcactttga aaaggctgtc
     1081 acccttgacc caaactttct ggatgcttat atcaatttag gaaatgtctt gaaagaggca
     1141 cgcatttttg acagagctgt ggcagcttat cttcgtgccc taagtttgag tccaaatcac
     1201 gcagtggtgc acggcaacct ggcttgtgta tactatgagc aaggcctgat agatctggca
     1261 atagacacct acaggcgggc tatcgaacta caaccacatt tccctgatgc ttactgcaac
     1321 ctagccaatg ctctcaaaga gaagggcagt gttgctgaag cagaagattg ttataataca
     1381 gctctccgtc tgtgtcccac ccatgcagac tctctgaata acctagccaa tatcaaacga
     1441 gaacagggaa acattgaaga ggcagttcgc ttgtatcgta aagcattaga agtcttccca
     1501 gagtttgctg ctgcccattc aaatttagca agtgtactgc agcagcaggg aaaactgcag
     1561 gaagctctga tgcattataa ggaggctatt cgaatcagtc ctacctttgc tgatgcctac
     1621 tctaatatgg gaaacactct aaaggagatg caggatgttc agggagcctt gcagtgttat
     1681 acgcgtgcca tccaaattaa tcctgcattt gcagatgcac atagcaatct ggcttccatt
     1741 cataaggatt cagggaatat tccagaagcc atagcttctt accgcacggc tctgaaactt
     1801 aagcctgatt ttcctgatgc ttattgtaac ttggctcatt gcctgcagat tgtctgtgat
     1861 tggacagact atgatgagcg aatgaagaag ttggtcagta ttgtggctga ccagttagag
     1921 aagaataggt tgccttctgt gcatcctcat catagtatgc tatatcctct ttctcatggc
     1981 ttcaggaagg ctattgctga gaggcacggc aacctgtgct tagataagat taatgttctt
     2041 cataaaccac catatgaaca tccaaaagac ttgaagctca gtgatggtcg gctgcgtgta
     2101 ggatatgtga gttccgactt tgggaatcat cctacttctc accttatgca gtctattcca
     2161 ggcatgcaca atcctgataa atttgaggtg ttctgttatg ccctgagccc agacgatggc
     2221 acaaacttcc gagtgaaggt gatggcagaa gccaatcatt tcattgatct ttctcagatt
     2281 ccatgcaatg gaaaagcagc tgatcgcatc catcaggatg gaattcatat ccttgtaaat
     2341 atgaatggct atactaaggg cgctcgaaat gagctttttg ctctcaggcc agctcctatt
     2401 caggcaatgt ggctgggata ccctgggacg agtggtgcgc ttttcatgga ttatattatc
     2461 actgatcagg aaacttcgcc agctgaagtt gctgagcagt attccgagaa attggcttat
     2521 atgccccaca ctttttttat tggtgatcat gctaatatgt tccctcacct gaagaaaaaa
     2581 gcagtcatcg attttaagtc caatgggcac atttatgaca atcggatagt tctgaatggc
     2641 atcgacctca aagcatttct tgatagtcta ccagatgtga aaattgtcaa gatgaagtgt
     2701 cctgatggag gagacaatgc agatagcagt aacacagctc ttaatatgcc tgttattcct
     2761 atgaatacta ttgcagaagc agttattgaa atgattaacc gaggacagat tcaaataaca
     2821 attaatggat tcagtattag caatggactg gcaactactc agatcaacaa taaggctgca
     2881 actggagagg aggttccccg taccattatt gtaaccaccc gttctcagta cgggttacca
     2941 gaagatgcca tcgtatactg taactttaat cagttgtata aaattgaccc ttctactttg
     3001 cagatgtggg caaacattct gaagcgtgtt cccaatagtg tactctggct gttgcgtttt
     3061 ccagcagtag gagaacctaa tattcaacag tatgcacaaa acatgggcct gccccagaac
     3121 cgtatcattt tttcacctgt tgctcctaaa gaggaacacg tcaggagagg ccagctggct
     3181 gatgtctgct tggacactcc actctgtaat gggcacacca cagggatgga tgtcctctgg
     3241 gcagggaccc ccatggtgac tatgccagga gagactcttg cttctcgagt tgcagcatcc
     3301 cagctcactt gcttaggttg tcttgagctt attgctaaaa acagacaaga atatgaagac
     3361 atagctgtga agctgggaac tgatctagaa tacctgaaga aagttcgtgg caaagtctgg
     3421 aagcaaagaa tatctagccc tctgttcaac accaaacaat acacaatgga actagagcgg
     3481 ctctatctac agatgtggga gcattatgca gctggcaaca aacctgacca catgattaag
     3541 cctgttgaag tcactgagtc agcataactc gagatatcta gacccagctt tcttgtacaa
     3601 agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag
     3661 tcaaaataaa atcattattt gccatccagc tgcagctctg gcccgtgtct caaaatctct
     3721 gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca
     3781 taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat
     3841 taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc
     3901 aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga
     3961 aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc
     4021 tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat
     4081 ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg
     4141 attcaggtga aaatattgtt gatgcgctgg cagtgtccct gcgccggttg cattcgattc
     4201 ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac
     4261 gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg
     4321 ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca
     4381 ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta
     4441 ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact
     4501 gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata
     4561 atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat
     4621 tggttaattg gttgtaacat tattcagatt gggccccgtt ccactgagcg tcagaccccg
     4681 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     4741 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     4801 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     4861 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     4921 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     4981 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     5041 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     5101 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     5161 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     5221 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     5281 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     5341 ttgctcacat gtt
//
