LOCUS       sequence-310423-        9471 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  plasmid with 4xMma PylT (M15 mutant, UUA anticodon) cassette and Mma
            PylRS for ochre suppression in transient or stable, 
            piggybac-mediated, integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9471)
  AUTHORS   Meineke B, Heimgartner J, Eirich J, Landreh M, Elsasser SJ
  TITLE     Site-Specific Incorporation of Two ncAAs for Two-Color Bioorthogonal
            Labeling and Crosslinking of Proteins on Live Mammalian Cells.
  JOURNAL   Cell Rep. 2020 Jun 23;31(12):107811. doi: 
            10.1016/j.celrep.2020.107811.
  PUBMED    32579937
REFERENCE   2  (bases 1 to 9471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2020-06-23"; volume: "31(12):107811. doi"; pages: " 
            10.1016/j.celrep.2020.107811"
COMMENT     Sequence Label: pAS_4xU6-PylT M15 (UUA) FLAG-Mma PylRS
FEATURES             Location/Qualifiers
     source          1..9471
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     127..149
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     141..158
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     142..158
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   288..350
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(870..1118)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        complement(1208..1456)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        complement(1546..1794)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        complement(1884..2132)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        2195..2406
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2419..2687
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      2719..2728
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2728..2751
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    join(4135..4709,4710..4712,4713..4721)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4302..4319)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4529..4548
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4734..5333
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4734..4753)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5230..5250
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(5393..5413)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    5455..5576
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5492..5511)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5546..5565
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   6339..6373
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6864..6880)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6864..6880)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6877..6899)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6888..6904
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6912..6918,6919..6936,6937..6942))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6957..6978
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7095..7112)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7266..7854)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7346..7365)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8025..8816,8817..8885))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8648..8667
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8886..8990)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9016..9471)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9153..9174)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9365..9384
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
      121 ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcctcg ttcattcacg
      181 tttttgaacc cgtggaggac gggcagactc gcggtgcaaa tgtgttttac agcgtgatgg
      241 agcagatgaa gatgctcgac acgctgcaga acacgcagct agattaaccc tagaaagata
      301 atcatattgt gacgtacgtt aaagataatc atgtgtaaaa ttgacgcatg tgttttatcg
      361 gtctgtatat cgaggtttat ttattaattt gaatagatat taagttttat tatatttaca
      421 cttacatact aataataaat tcaacaaaca atttatttat gtttatttat ttattaaaaa
      481 aaacaaaaac tcaaaatttc ttctataaag taacaaaact tttatgaggg acagcccccc
      541 cccaaagccc ccagggatgt aattacgtcc ctcccccgct agggggcagc agcgagccgc
      601 ccggggctcc gctccggtcc ggcgctcccc ccgcatcccc gagccggcag cgtgcgggga
      661 cagcccgggc acggggaagg tggcacggga tcgctttcct ctgaacgctt ctcgctgctc
      721 tttgagcctg cagacacctg gggggatacg gggaaaaggc ctccacggcc aactagtgag
      781 acgtaaaaac cgcacttgtc cggaaacccc gggaatcgaa cccggctgaa cggatttaaa
      841 gtccgttcgg tctccctgac caggtttccg gtgtttcgtc ctttccacaa gatatataaa
      901 gccaagaaat cgaaatactt tcaagttacg gtaagcatat gatagtccat tttaaaacat
      961 aattttaaaa ctgcaaacta cccaagaaat tattactttc tacgtcacgt attttgtact
     1021 aatatctttg tgtttacagt caaattaatt ctaattatct ctctaacagc cttgtatcgt
     1081 atatgcaaat atgaaggaat catgggaaat aggccctcgc aaaaaaaccg cacttgtccg
     1141 gaaaccccgg gaatcgaacc cggctgaacg gatttaaagt ccgttcggtc tccctgacca
     1201 ggtttccggt gtttcgtcct ttccacaaga tatataaagc caagaaatcg aaatactttc
     1261 aagttacggt aagcatatga tagtccattt taaaacataa ttttaaaact gcaaactacc
     1321 caagaaatta ttactttcta cgtcacgtat tttgtactaa tatctttgtg tttacagtca
     1381 aattaattct aattatctct ctaacagcct tgtatcgtat atgcaaatat gaaggaatca
     1441 tgggaaatag gccctccaag aaaaaccgca cttgtccgga aaccccggga atcgaacccg
     1501 gctgaacgga tttaaagtcc gttcggtctc cctgaccagg tttccggtgt ttcgtccttt
     1561 ccacaagata tataaagcca agaaatcgaa atactttcaa gttacggtaa gcatatgata
     1621 gtccatttta aaacataatt ttaaaactgc aaactaccca agaaattatt actttctacg
     1681 tcacgtattt tgtactaata tctttgtgtt tacagtcaaa ttaattctaa ttatctctct
     1741 aacagccttg tatcgtatat gcaaatatga aggaatcatg ggaaataggc cctccgataa
     1801 aaaccgcact tgtccggaaa ccccgggaat cgaacccggc tgaacggatt taaagtccgt
     1861 tcggtctccc tgaccaggtt tccggtgttt cgtcctttcc acaagatata taaagccaag
     1921 aaatcgaaat actttcaagt tacggtaagc atatgatagt ccattttaaa acataatttt
     1981 aaaactgcaa actacccaag aaattattac tttctacgtc acgtattttg tactaatatc
     2041 tttgtgttta cagtcaaatt aattctaatt atctctctaa cagccttgta tcgtatatgc
     2101 aaatatgaag gaatcatggg aaataggccc tcctccaagc ctagggccac tagtgctcta
     2161 gaaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     2221 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg
     2281 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     2341 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     2401 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga
     2461 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga
     2521 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg
     2581 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct
     2641 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg
     2701 gcgcctactc tagagctagc caccatggac tacaaggacg acgacgacaa gatggacaag
     2761 aagcccctga acaccctgat cagcgccaca ggactgtgga tgtccagaac cggcaccatc
     2821 cacaagatca agcaccacga ggtgtcccgg tccaaaatct acatcgagat ggcctgcggc
     2881 gatcacctgg tcgtcaacaa cagcagaagc agccggacag ccagagccct gcggcaccac
     2941 aagtacagaa agacctgcaa gcggtgcaga gtgtccgacg aggacctgaa caagttcctg
     3001 accaaggcca acgaggacca gaccagcgtg aaagtgaagg tggtgtccgc ccccacccgg
     3061 accaagaaag ccatgcccaa gagcgtggcc agagccccca agcccctgga aaacaccgaa
     3121 gccgctcagg cccagcccag cggcagcaag ttcagccccg ccatccccgt gtctacccag
     3181 gaaagcgtca gcgtccccgc cagcgtgtcc accagcatct ctagcatctc aaccggcgcc
     3241 acagcttctg ccctggtcaa gggcaacacc aaccccatca ccagcatgtc tgcccctgtg
     3301 caggcctctg ccccagccct gaccaagtcc cagaccgacc ggctggaagt gctcctgaac
     3361 cccaaggacg agatcagcct gaacagcggc aagcccttcc gggagctgga aagcgagctg
     3421 ctgagccggc ggaagaagga cctccagcaa atctacgccg aggaacggga gaactacctg
     3481 ggcaagctgg aaagagagat cacccggttc ttcgtggacc ggggcttcct ggaaatcaag
     3541 agccccatcc tgatccccct ggagtacatc gagcggatgg gcatcgacaa cgacaccgag
     3601 ctgagcaagc agattttccg ggtggacaag aacttctgcc tgcggcccat gctggccccc
     3661 aacctgtaca actacctgcg gaaactggat cgcgctctgc ccgaccccat caagattttc
     3721 gagatcggcc cctgctaccg gaaagagagc gacggcaaag agcacctgga agagtttaca
     3781 atgctgaact tttgccagat gggcagcggc tgcaccagag agaacctgga atccatcatc
     3841 accgactttc tgaaccacct ggggatcgac ttcaagatcg tgggcgacag ctgcatggtg
     3901 tacggcgaca ccctggacgt gatgcacggc gacctggaac tgtctagcgc cgtcgtggga
     3961 cccatccctc tggaccggga gtggggcatc gataagccct ggatcggagc cggcttcggc
     4021 ctggaacggc tgctgaaagt caagcacgac tttaagaaca tcaagcgggc tgccagaagc
     4081 gagagctact acaacggcat cagcaccaac ctgtgatgag gatccgcggc cgcgcccctc
     4141 tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
     4201 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
     4261 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
     4321 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
     4381 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
     4441 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
     4501 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
     4561 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg
     4621 ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt
     4681 ggttttcctt tgaaaaacac gatgataata tggccacaac catggcgtcc ggaatgaccg
     4741 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
     4801 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
     4861 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     4921 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
     4981 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     5041 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     5101 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     5161 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     5221 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     5281 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgagtcgaca
     5341 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     5401 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgcgtta actaaacttg
     5461 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     5521 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     5581 gtctggaatt gactcaaatg atgtcaatta gtctatcaga agctcatctg gtctcccttc
     5641 cgggggacaa gacatccctg tttaatattt aaacagcagt gttcccaaac tgggttctta
     5701 tatcccttgc tctggtcaac caggttgcag ggtttcctgt cctcacagga acgaagtccc
     5761 taaagaaaca gtggcagcca ggtttagccc cggaattgac tggattcctt ttttagggcc
     5821 cattggtatg gctttttccc cgtatccccc caggtgtctg caggctcaaa gagcagcgag
     5881 aagcgttcag aggaaagcga tcccgtgcca ccttccccgt gcccgggctg tccccgcacg
     5941 ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg gcggctcgct
     6001 gctgccccct agcgggggag ggacgtaatt acatccctgg gggctttggg ggggggctgt
     6061 ccctgatatc tataacaaga aaatatatat ataataagtt atcacgtaag tagaacatga
     6121 aataacaata taattatcgt atgagttaaa tcttaaaagt cacgtaaaag ataatcatgc
     6181 gtcattttga ctcacgcggt cgttatagtt caaaatcagt gacacttacc gcattgacaa
     6241 gcacgcctca cgggagctcc aagcggcgac tgagatgtcc taaatgcaca gcgacggatt
     6301 cgcgctattt agaaagagag agcaatattt caagaatgca tgcgtcaatt ttacgcagac
     6361 tatctttcta gggttaatct agctgcatca ggatcatatc gtcgggtctt ttttccggct
     6421 cagtcatcgc ccaagctggc gctatctggg catcggggag gaagaagccc gtgccttttc
     6481 ccgcgaggtt gaagcggcat ggaaagagtt tgccgaggat gactgctgct gcattgacgt
     6541 tgagcgaaaa cgcacgttta ccatgatgat tcgggaaggt gtggccatgc acgcctttaa
     6601 cggtgaactg ttcgttcagg ccacctggga taccagttcg tcgcggcttt tccggacaca
     6661 gttccggatg gtcagcccga agcgcatcag caacccgaac aataccggcg acagccggaa
     6721 ctgccgtgcc ggtgtgcaga ttaatgacag cggtgcggcg ctgggatatt acgtcagcga
     6781 ggacgggtat cctggctgga tgccgcagaa atggacatgg ataccccgtg agttacccgg
     6841 cgggcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     6901 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     6961 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     7021 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     7081 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     7141 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     7201 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     7261 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     7321 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     7381 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     7441 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     7501 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     7561 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     7621 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     7681 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     7741 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     7801 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     7861 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     7921 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     7981 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     8041 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     8101 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     8161 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     8221 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     8281 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     8341 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     8401 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     8461 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     8521 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     8581 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     8641 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     8701 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     8761 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     8821 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     8881 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     8941 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     9001 cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     9061 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     9121 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     9181 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     9241 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     9301 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     9361 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     9421 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg t
//
