LOCUS       sequence-310428-        9077 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  plasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid mutant 
            A41AA C55A) and G1 PylRS Y125A for amber suppression in transient or
            stable, piggybac-mediated, integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9077)
  AUTHORS   Meineke B, Heimgartner J, Eirich J, Landreh M, Elsasser SJ
  TITLE     Site-Specific Incorporation of Two ncAAs for Two-Color Bioorthogonal
            Labeling and Crosslinking of Proteins on Live Mammalian Cells.
  JOURNAL   Cell Rep. 2020 Jun 23;31(12):107811. doi: 
            10.1016/j.celrep.2020.107811.
  PUBMED    32579937
REFERENCE   2  (bases 1 to 9077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2020-06-23"; volume: "31(12):107811. doi"; pages: " 
            10.1016/j.celrep.2020.107811"
COMMENT     Sequence Label: pAS_4xhybPylT A41AA C55A FLAG-G1 PylRS Y125A
FEATURES             Location/Qualifiers
     source          1..9077
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     127..149
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     141..158
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     142..158
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   288..350
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        2164..2375
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2388..2656
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      2688..2697
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2697..2720
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    3564..4136
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3731..3748)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3958..3977
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4145..4939
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLAATGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4199..4218)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4809..4828
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4999..5019)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    5061..5182
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5098..5117)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5152..5171
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   5945..5979
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6470..6486)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6470..6486)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6483..6505)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6494..6510
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6518..6524,6525..6542,6543..6548))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6563..6584
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6701..6718)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6872..7460)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6952..6971)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7631..8422,8423..8491))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8254..8273
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8492..8596)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8622..9077)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8759..8780)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8971..8990
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
      121 ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcctcg ttcattcacg
      181 tttttgaacc cgtggaggac gggcagactc gcggtgcaaa tgtgttttac agcgtgatgg
      241 agcagatgaa gatgctcgac acgctgcaga acacgcagct agattaaccc tagaaagata
      301 atcatattgt gacgtacgtt aaagataatc atgtgtaaaa ttgacgcatg tgttttatcg
      361 gtctgtatat cgaggtttat ttattaattt gaatagatat taagttttat tatatttaca
      421 cttacatact aataataaat tcaacaaaca atttatttat gtttatttat ttattaaaaa
      481 aaacaaaaac tcaaaatttc ttctataaag taacaaaact tttatgaggg acagcccccc
      541 cccaaagccc ccagggatgt aattacgtcc ctcccccgct agggggcagc agcgagccgc
      601 ccggggctcc gctccggtcc ggcgctcccc ccgcatcccc gagccggcag cgtgcgggga
      661 cagcccgggc acggggaagg tggcacggga tcgctttcct ctgaacgctt ctcgctgctc
      721 tttgagcctg cagacacctg gggggatacg gggaaaaggc ctccacggcc aactagtgag
      781 acgaaaaacc gcacttgtcc gagagaccgg ggtgtcgaac cccgctttac aggttttaga
      841 gacccgtttg ctcgccggag cgtcccccga ggtacccagg cggcgcacaa gctatataaa
      901 cctgaaggaa atctcaactt tacacttagg tcaagttact tatcgtacta gagcttcagc
      961 aggaaattta actaaaatct aatttaacca gcatagcaaa tatcatttat tcccaaaatg
     1021 ctaaagtttg agataaacgg acttgatttc cggctgtttt gacactatcc agaatgcctt
     1081 gcagatgggt ggggcatgct aaatactgca ggcaaaaaaa ccgcacttgt ccgagagacc
     1141 ggggtgtcga accccgcttt acaggtttta gagacccgtt tgctcgccgg agcgtccccc
     1201 gaggtaccca ggcggcgcac aagctatata aacctgaagg aaatctcaac tttacactta
     1261 ggtcaagtta cttatcgtac tagagcttca gcaggaaatt taactaaaat ctaatttaac
     1321 cagcatagca aatatcattt attcccaaaa tgctaaagtt tgagataaac ggacttgatt
     1381 tccggctgtt ttgacactat ccagaatgcc ttgcagatgg gtggggcatg ctaaatactg
     1441 cagcaagaaa aaccgcactt gtccgagaga ccggggtgtc gaaccccgct ttacaggttt
     1501 tagagacccg tttgctcgcc ggagcgtccc ccgaggtacc caggcggcgc acaagctata
     1561 taaacctgaa ggaaatctca actttacact taggtcaagt tacttatcgt actagagctt
     1621 cagcaggaaa tttaactaaa atctaattta accagcatag caaatatcat ttattcccaa
     1681 aatgctaaag tttgagataa acggacttga tttccggctg ttttgacact atccagaatg
     1741 ccttgcagat gggtggggca tgctaaatac tgcagcgata aaaaccgcac ttgtccgaga
     1801 gaccggggtg tcgaaccccg ctttacaggt tttagagacc cgtttgctcg ccggagcgtc
     1861 ccccgaggta cccaggcggc gcacaagcta tataaacctg aaggaaatct caactttaca
     1921 cttaggtcaa gttacttatc gtactagagc ttcagcagga aatttaacta aaatctaatt
     1981 taaccagcat agcaaatatc atttattccc aaaatgctaa agtttgagat aaacggactt
     2041 gatttccggc tgttttgaca ctatccagaa tgccttgcag atgggtgggg catgctaaat
     2101 actgcagctc caaggccact agtgctctag aaaggatctg cgatcgctcc ggtgcccgtc
     2161 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt
     2221 gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
     2281 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
     2341 ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg gctcgcatct
     2401 ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg agtcgcgttc
     2461 tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc
     2521 tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga ctcagccggc
     2581 tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg ttttctgttc
     2641 tgcgccgtta cagatccaag ctgtgaccgg cgcctactct agagctagcc accatggact
     2701 acaaggacga cgacgacaag gtggtcaagt tcaccgacag ccagatccag cacctgatgg
     2761 aatacggcga caacgattgg agcgaggccg agttcgaaga tgccgccgct agagacaaag
     2821 agttcagcag ccagttcagc aagctgaagt ccgccaacga caagggcctg aaggacgtga
     2881 tcgccaatcc tcggaacgac ctgaccgacc tggaaaacaa gatcagagag aagctggccg
     2941 ccagaggctt catcgaggtg cacaccccta tcttcgtgtc caagtctgcc ctggccaaga
     3001 tgaccatcac cgaggatcac cctctgttca aacaggtgtt ctggatcgac gacaagcggg
     3061 ccctgaggcc tatgcacgcc atgaacctgg ccaaagtgat gagagagctg cgggaccaca
     3121 caaagggccc cgtgaagatc tttgagatcg gcagctgctt ccggaaagag agcaagagca
     3181 gcacccacct ggaagagttc accatgctga acctggtgga aatgggcccc gacggcgatc
     3241 ctatggaaca cctgaagatg tatatcggcg acatcatgga cgccgtgggc gtcgagtaca
     3301 ccaccagcag agaagagtcc gacgtgtacg tggaaaccct ggacgtggaa atcaacggca
     3361 ccgaagtggc tagcggagcc gtgggacctc acaaactgga tcctgctcac gacgtgcacg
     3421 aaccttgggc cggaatcggc tttggcctgg aaagactgct gatgctgaag aacggcaaga
     3481 gcaacgccag aaagaccggc aagtccatca cctacctgaa cggctacaag ctggactgat
     3541 gaatcgatgg atccgcggcc gcgcccctct ccctcccccc cccctaacgt tactggccga
     3601 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
     3661 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
     3721 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
     3781 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
     3841 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
     3901 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
     3961 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
     4021 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa
     4081 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg ataatacctc
     4141 cggaatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     4201 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     4261 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     4321 actgcaggac gaggcagcgc ggctatcgtg gctggccgcg acgggcgttc cttgcgcagc
     4381 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     4441 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     4501 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     4561 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     4621 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
     4681 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     4741 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     4801 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     4861 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     4921 tcttgacgag ttcttctgag tcgacaatca acctctggat tacaaaattt gtgaaagatt
     4981 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     5041 tttgtatcat gcgttaacta aacttgttta ttgcagctta taatggttac aaataaagca
     5101 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     5161 ccaaactcat caatgtatct tatcatgtct ggaattgact caaatgatgt caattagtct
     5221 atcagaagct catctggtct cccttccggg ggacaagaca tccctgttta atatttaaac
     5281 agcagtgttc ccaaactggg ttcttatatc ccttgctctg gtcaaccagg ttgcagggtt
     5341 tcctgtcctc acaggaacga agtccctaaa gaaacagtgg cagccaggtt tagccccgga
     5401 attgactgga ttcctttttt agggcccatt ggtatggctt tttccccgta tccccccagg
     5461 tgtctgcagg ctcaaagagc agcgagaagc gttcagagga aagcgatccc gtgccacctt
     5521 ccccgtgccc gggctgtccc cgcacgctgc cggctcgggg atgcgggggg agcgccggac
     5581 cggagcggag ccccgggcgg ctcgctgctg ccccctagcg ggggagggac gtaattacat
     5641 ccctgggggc tttggggggg ggctgtccct gatatctata acaagaaaat atatatataa
     5701 taagttatca cgtaagtaga acatgaaata acaatataat tatcgtatga gttaaatctt
     5761 aaaagtcacg taaaagataa tcatgcgtca ttttgactca cgcggtcgtt atagttcaaa
     5821 atcagtgaca cttaccgcat tgacaagcac gcctcacggg agctccaagc ggcgactgag
     5881 atgtcctaaa tgcacagcga cggattcgcg ctatttagaa agagagagca atatttcaag
     5941 aatgcatgcg tcaattttac gcagactatc tttctagggt taatctagct gcatcaggat
     6001 catatcgtcg ggtctttttt ccggctcagt catcgcccaa gctggcgcta tctgggcatc
     6061 ggggaggaag aagcccgtgc cttttcccgc gaggttgaag cggcatggaa agagtttgcc
     6121 gaggatgact gctgctgcat tgacgttgag cgaaaacgca cgtttaccat gatgattcgg
     6181 gaaggtgtgg ccatgcacgc ctttaacggt gaactgttcg ttcaggccac ctgggatacc
     6241 agttcgtcgc ggcttttccg gacacagttc cggatggtca gcccgaagcg catcagcaac
     6301 ccgaacaata ccggcgacag ccggaactgc cgtgccggtg tgcagattaa tgacagcggt
     6361 gcggcgctgg gatattacgt cagcgaggac gggtatcctg gctggatgcc gcagaaatgg
     6421 acatggatac cccgtgagtt acccggcggg cgcgcttggc gtaatcatgg tcatagctgt
     6481 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     6541 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     6601 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     6661 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     6721 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     6781 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     6841 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     6901 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6961 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     7021 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     7081 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     7141 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     7201 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     7261 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     7321 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7381 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     7441 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     7501 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     7561 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     7621 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     7681 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     7741 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     7801 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     7861 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     7921 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     7981 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     8041 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     8101 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     8161 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     8221 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     8281 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     8341 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     8401 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     8461 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     8521 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     8581 aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat
     8641 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     8701 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     8761 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     8821 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     8881 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     8941 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     9001 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     9061 gccgctacag ggcgcgt
//
