LOCUS       sequence-310431-        8897 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8897)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 8897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: RXFP2-Tango
FEATURES             Location/Qualifiers
     source          1..8897
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(3221..3835,3836..4222)
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="RLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVKN
                     KRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRPT
                     EKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDS
                     MPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTIEG
                     LLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHADA
                     LDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(3288..3307)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(4254..4271)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4260..4484
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4530..4958
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4617..4636)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4827..4848
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4967..4987)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4972..5301
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5152..5287
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5214..5233
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5368..6162
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5422..5441)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6032..6051
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6336..6457
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6373..6392)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6427..6446
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6506..6522)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6506..6522)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6519..6541)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6530..6546
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6554..6560,6561..6578,6579..6584))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6599..6620
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6737..6754)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6908..7496)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6988..7007)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7667..8458,8459..8527))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8290..8309
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8528..8632)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8707..8726)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttggtaccga gctcggatcc actagtccag tgtggtggaa
      721 ttctgcagat atccagcaca gtggcggccg cgccaccatg aagacgatca tcgccctgag
      781 ctacatcttc tgcctggtat tcgccgacta caaggacgat gatgacgcca gcatcgatat
      841 gatcgtcttc ctcgtgttca agcacctgtt ttccctccgc ttgataacaa tgttctttct
      901 cctccatttc atcgtgctga ttaacgtcaa ggattttgct ctgacacagg ggagcatgat
      961 cacgcccagc tgccagaagg gatatttccc gtgcggtaac ctgactaaat gcctgccacg
     1021 cgcattccat tgcgacggaa aggacgactg cggcaacggc gcggacgagg agaactgcgg
     1081 cgataccagt gggtgggcca ccatctttgg aacagttcac gggaacgcca atagcgtggc
     1141 cttgacccaa gagtgttttc tgaaacagta tcctcagtgc tgcgattgca aagagacaga
     1201 attggagtgc gtgaatgggg acttgaaatc cgtacctatg atttccaaca atgtgacact
     1261 tctttccctg aagaagaaca aaatacacag cctgccggac aaagtcttca tcaaatacac
     1321 taagttgaaa aagatcttcc ttcagcacaa ttgcatacgg cacatatcta gaaaagcctt
     1381 ttttggtctg tgcaatcttc agattctgta cttgaatcac aactgtatta cgactctgag
     1441 accaggcata tttaaggacc tccaccaact gacctggctt atactcgatg acaacccaat
     1501 tactcggatc tcacagcgac tgtttacggg cctgaactcc ctctttttct tgtccatggt
     1561 caataactac cttgaagcct tgcccaaaca gatgtgtgca cagatgccgc agcttaactg
     1621 ggtcgatctg gaggggaaca ggatcaagta cctgaccaat agcacttttt tgagctgtga
     1681 ttctctgacc gtgctcttcc tcccacggaa tcaaatcggc ttcgttccag aaaagacgtt
     1741 cagtagtctc aagaacctgg gagagctcga tctgtccagc aatacgatta ctgaactgtc
     1801 tcctcacctg tttaaggacc tgaaactcct gcagaagctg aacctgtcaa gtaatcccct
     1861 gatgtacctg cacaagaatc agttcgagtc actcaagcag ctccaatcat tggacctgga
     1921 gaggatcgaa atccccaata taaacacaag gatgtttcag ccaatgaaga acctctctca
     1981 catctatttc aagaatttta ggtattgtag ttatgccccc catgttcgga tctgtatgcc
     2041 tcttacagat ggcatttcct ccttcgagga cctgctggcc aacaatatcc tgcgaatctt
     2101 cgtctgggtc atagccttca ttacctgctt cggaaacctg ttcgtgattg gaatgaggag
     2161 ttttattaag gccgaaaaca ctacacatgc aatgagcatc aaaatcctgt gctgtgccga
     2221 ctgccttatg ggtgtatact tgttcttcgt aggcatattc gatatcaaat atcggggtca
     2281 gtatcaaaag tacgcacttc tgtggatgga gtccgtgcag tgtcgcctga tgggatttct
     2341 cgccatgctg agtactgagg ttagtgtgct cttgctgaca taccttacct tggaaaaatt
     2401 tctggtgatt gtctttccct tctccaatat tcgccccggg aagcgccaga cctccgtaat
     2461 actgatctgt atttggatgg ccgggttcct gatagccgtg atcccgttct ggaacaagga
     2521 ttacttcggc aacttctatg gcaagaatgg ggtctgcttt ccactttatt acgaccagac
     2581 cgaagacatc ggcagcaagg gatactctct tggcatcttc cttggggtga acctcctcgc
     2641 cttcctgatt attgtgttca gctatattac catgttttgc tccatccaga aaactgcact
     2701 tcagactacg gaggtgagaa attgcttcgg cagagaagtg gctgtggcca atcggttttt
     2761 ttttatcgtg tttagtgacg ctatctgttg gatccctgtg tttgtagtca aaatactgag
     2821 cctcttccgg gttgagatac ctgacacaat gacctcctgg attgtgatat tttttcttcc
     2881 cgttaacagt gccttgaacc caatactcta tacactgact actaattttt ttaaagataa
     2941 actcaaacag ctgctgcaca agcaccagcg gaagagcatt tttaaaatta aaaagaaaag
     3001 cctgagtacc tccattgttt ggattgagga cagtagcagt ttgaaactgg gggtgctcaa
     3061 caagataact ctgggcgaca gtatcatgaa gccggttagc atcgataccg gtggacgcac
     3121 cccacccagc ctgggtcccc aagatgagtc ctgcaccacc gccagctcct ccctggccaa
     3181 ggacacttca tcgaccggtg agaacctgta cttccagcta agattagata aaagtaaagt
     3241 gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa
     3301 actcgcccag aagctaggtg tagagcagcc tacattgtat tggcatgtaa aaaataagcg
     3361 ggctttgctc gacgccttag ccattgagat gttagatagg caccatactc acttttgccc
     3421 tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt ttagatgtgc
     3481 tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta cagaaaaaca
     3541 gtatgaaact ctcgaaaatc aattagcctt tttatgccaa caaggttttt cactagagaa
     3601 tgcattatat gcactcagcg ctgtggggca ttttacttta ggttgcgtat tggaagatca
     3661 agagcatcaa gtcgctaaag aagaaaggga aacacctact actgatagta tgccgccatt
     3721 attacgacaa gctatcgaat tatttgatca ccaaggtgca gagccagcct tcttattcgg
     3781 ccttgaattg atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg ggtccgcgta
     3841 cagccgcgcg cgtacgaaaa acaattacgg gtctaccatc gagggcctgc tcgatctccc
     3901 ggacgacgac gcccccgaag aggcggggct ggcggctccg cgcctgtcct ttctccccgc
     3961 gggacacacg cgcagactgt cgacggcccc cccgaccgat gtcagcctgg gggacgagct
     4021 ccacttagac ggcgaggacg tggcgatggc gcatgccgac gcgctagacg atttcgatct
     4081 ggacatgttg ggggacgggg attccccggg tccgggattt accccccacg actccgcccc
     4141 ctacggcgct ctggatatgg ccgacttcga gtttgagcag atgtttaccg atgcccttgg
     4201 aattgacgag tacggtgggt agtctagagg gcccgtttaa acccgctgat cagcctcgac
     4261 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     4321 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     4381 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     4441 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     4501 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     4561 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     4621 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     4681 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     4741 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     4801 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     4861 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     4921 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     4981 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     5041 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     5101 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     5161 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     5221 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     5281 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     5341 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
     5401 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     5461 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     5521 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
     5581 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     5641 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     5701 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     5761 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     5821 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     5881 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     5941 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     6001 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     6061 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     6121 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
     6181 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct
     6241 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     6301 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     6361 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     6421 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     6481 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     6541 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     6601 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     6661 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     6721 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     6781 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     6841 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     6901 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     6961 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     7021 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     7081 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     7141 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     7201 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     7261 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     7321 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     7381 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     7441 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     7501 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     7561 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     7621 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     7681 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     7741 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     7801 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     7861 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     7921 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     7981 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     8041 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     8101 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     8161 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     8221 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     8281 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     8341 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     8401 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     8461 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     8521 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     8581 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     8641 cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac
     8701 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
     8761 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
     8821 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg
     8881 ggccagatat acgcgtt
//
