LOCUS       sequence-310434-        9656 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human MUC2 residues 21-1397.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9656)
  AUTHORS   Javitt G, Khmelnitsky L, Albert L, Bigman LS, Elad N, Morgenstern D,
            Ilani T, Levy Y, Diskin R, Fass D
  TITLE     Assembly Mechanism of Mucin and von Willebrand Factor Polymers.
  JOURNAL   Cell. 2020 Oct 29;183(3):717-729.e16. doi: 
            10.1016/j.cell.2020.09.021. Epub 2020 Oct 7.
  PUBMED    33031746
REFERENCE   2  (bases 1 to 9656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.09.021"; journalName: "Cell"; date: 
            "2020-10-29- 29"; volume: "183"; issue: "3"; pages: "717-729.e16"
COMMENT     Sequence Label: pCDNA3.1-Muc2D1D2D3cysD1
FEATURES             Location/Qualifiers
     source          1..9656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4922..4939
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5013..5030)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5019..5243
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5289..5717
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5376..5395)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5586..5607
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5726..5746)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5731..6060
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5911..6046
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5973..5992
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6127..6921
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6181..6200)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6791..6810
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7095..7216
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7132..7151)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7186..7205
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7265..7281)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7265..7281)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7278..7300)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7289..7305
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7313..7319,7320..7337,7338..7343))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7358..7379
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7496..7513)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7667..8255)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7747..7766)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8426..9217,9218..9286))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9049..9068
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9287..9391)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9466..9485)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttggtaccga gctcggatcc atgaggaggt gcaacagcgg
      721 ctccgggccg ccgccgtcgc tgctgctgct gctgtggctg ctcgcggttc ccggcgctaa
      781 cgcggccccg tcggagctcc agacagaggg cagaacccga aaccacggcc acaacgtctg
      841 cagcacctgg ggcaacttcc actacaagac cttcgacggg gacgtcttcc gcttccccgg
      901 cctctgcgac tacaacttcg cctccgactg ccgaggctcc tacaaggaat ttgctgtgca
      961 cctgaagcgg ggtccgggcc aggctgaggc ccccgccggg gtggagtcca tcctgctgac
     1021 catcaaggat gacaccatct acctcacccg ccacctggct gtgcttaacg gggccgtggt
     1081 cagcaccccg cactacagcc ccgggctgct cattgagaag agcgatgcct acaccaaagt
     1141 ctactcccgc gccggcctca ccctcatgtg gaaccgggag gatgcactca tgctggagct
     1201 ggacactaag ttccggaacc acacctgtgg cctctgcggg gactacaacg gcctgcagag
     1261 ctattcagaa ttcctctctg acggcgtgct cttcagtccc ctggagtttg ggaacatgca
     1321 gaagatcaac cagcccgatg tggtgtgtga ggatcccgag gaggaggtgg cccccgcatc
     1381 ctgctccgag caccgcgccg agtgtgagag gctgctgacc gccgaggcct tcgcggactg
     1441 tcaggacctg gtgccgctgg agccgtatct gcgcgcctgc cagcaggacc gctgccggtg
     1501 cccgggcggt gacacctgcg tctgcagcac cgtggccgag ttctcccgcc agtgctccca
     1561 cgccggcggc cggcccggga actggaggac cgccacgctc tgccccaaga cctgccccgg
     1621 gaacctggtg tacctggaga gcggctcgcc ctgcatggac acctgctcac acctggaggt
     1681 gagcagcctg tgcgaggagc accgcatgga cggctgtttc tgcccagaag gcaccgtata
     1741 tgacgacatc ggggacagtg gctgcgttcc tgtgagccag tgccactgca ggctgcacgg
     1801 acacctgtac acaccgggcc aggagatcac caatgactgc gagcagtgtg tctgtaacgc
     1861 tggccgctgg gtgtgcaaag acctgccctg ccccggcacc tgtgccctgg aaggcggctc
     1921 ccacatcacc accttcgatg ggaagacgta caccttccac ggggactgct actatgtcct
     1981 ggccaagggt gaccacaacg attcctatgc tctcctgggc gagctggccc cctgtggctc
     2041 cacagacaag cagacctgcc tgaagacggt ggtgctgctg gctgacaaga agaagaatgt
     2101 ggtggtcttc aagtccgatg gcagtgtact gctcaacgag ctgcaggtga acctgcccca
     2161 cgtgaccgcg agcttctctg tcttccgccc gtcttcctac cacatcatgg tgagcatggc
     2221 cattggcgtc cggctgcagg tgcagctggc cccagtcatg caactctttg tgacactgga
     2281 ccaggcctcc caggggcagg tgcagggcct ctgcgggaac ttcaacggcc tggaaggtga
     2341 cgacttcaag acggccagcg ggctggtgga ggccacgggg gccggctttg ccaacacctg
     2401 gaaggcacag tcaagctgcc atgacaagct ggactggttg gacgatccct gctccctgaa
     2461 catcgagagc gccaactacg ccgagcactg gtgctccctc ctgaagaaga cagagacccc
     2521 ctttggcagg tgccactcgg ctgtggaccc tgctgagtat tacaagaggt gcaaatatga
     2581 cacgtgtaac tgtcagaaca atgaggactg cctgtgcgcc gccctgtcct cctacgcgcg
     2641 cgcctgcacc gccaagggcg tcatgctgtg gggctggcgg gagcatgtct gcaacaagga
     2701 tgtgggctcc tgccccaact cgcaggtctt cctgtacaac ctgaccacct gccagcagac
     2761 ctgccgctcc ctctccgagg ccgacagcca ctgtctcgag ggctttgcgc ctgtggacgg
     2821 ctgcggctgc cctgaccaca ccttcctgga cgagaagggc cgctgcgtac ccctggccaa
     2881 gtgctcctgt taccaccgcg gtctctacct ggaggcgggg gatgtggtcg tcaggcagga
     2941 agaacgatgt gtgtgccggg atgggaggct gcactgtagg cagatccggc tgatcggcca
     3001 gagctgcacg gccccaaaga tccacatgga ctgcagcaac ctgactgcac tggccacctc
     3061 gaagccccga gccctcagct gccagacgct ggccgccggc tattaccaca cagagtgtgt
     3121 cagtggctgt gtgtgccccg acgggctgat ggatgacggc cggggtggct gtgtggtgga
     3181 gaaggaatgc ccttgcgtcc ataacaacga cctgtattct tccggcgcca agatcaaggt
     3241 ggactgcaat acctgcacct gcaagagagg acgctgggtg tgcacccagg ctgtgtgcca
     3301 tggcacctgc tccatttacg ggagtggcca ctacatcacc tttgatggga agtactacga
     3361 ctttgacgga cactgctcct acgtggctgt tcaggactac tgcggccaga actcctcact
     3421 gggctcattc agcatcatca ccgagaacgt cccctgtggc actacgggcg tcacctgctc
     3481 caaggccatt aagatcttca tggggaggac ggagctgaag ttggaagaca agcaccgtgt
     3541 ggtgatccag cgtgatgagg gtcaccacgt ggcctacacc acgcgggagg tgggccagta
     3601 cctggtggtg gagtccagca cgggcatcat cgtcatctgg gacaagagga ccaccgtgtt
     3661 catcaagctg gctccctcct acaagggcac cgtgtgtggc ctgtgtggga actttgacca
     3721 ccgctccaac aacgacttca ccacgcggga ccacatggtg gtgagcagcg agctggactt
     3781 cgggaacagc tggaaggagg cccccacctg cccagatgtg agcaccaacc ccgagccctg
     3841 cagcctgaac ccgcaccgcc gctcctgggc cgagaagcag tgcagcatcc tcaaaagcag
     3901 cgtgttcagc atctgccaca gcaaggtgga ccccaagccc ttctacgagg cctgtgtgca
     3961 cgactcgtgc tcctgtgaca cgggtgggga ctgtgagtgc ttctgctctg ccgtggcctc
     4021 ctacgcccag gagtgtacca aagagggggc ctgcgtgttc tggaggacgc cggacctgtg
     4081 ccccatattc tgcgactact acaaccctcc gcatgagtgt gagtggcact atgagccatg
     4141 tgggaaccgg agcttcgaga cctgcaggac catcaacggc atccactcca acatctccgt
     4201 gtcctacctg gagggctgct acccccggtg ccccaaggac aggcccatct atgaggagga
     4261 tctgaagaag tgtgtcactg cagacaagtg tggctgctat gtcgaggaca cccactaccc
     4321 acctggagca tcggttccca ccgaggagac ctgcaagtcc tgcgtgtgta ccaactcctc
     4381 ccaagtcgtc tgcaggccgg aggaaggaaa gattcttaac cagacccagg atggcgcctt
     4441 ctgctactgg gagatctgtg gccccaacgg gacggtggag aagcacttca acatctgttc
     4501 cattacgaca cgcccgtcca ccctgaccac cttcaccacc atcaccctcc ccaccacccc
     4561 cacctccttc accactacca ccaccaccac caccccgacc tccagcacag ttttatcaac
     4621 aactccgaag ctgtgctgcc tctggtctga ctggatcaat gaggaccacc ccagcagtgg
     4681 cagcgacgac ggtgaccgag aaacatttga tggggtctgc ggggcccctg aggacatcga
     4741 gtgcaggtcg gtcaaggatc cccacctcag cttggagcag ctaggccaga aggtgcagtg
     4801 tgatgtctct gttgggttca tttgcaagaa tgaagaccag tttggaaatg gaccatttgg
     4861 actgtgttac gactacaaga tacgtgtcaa ttgttgctgg cccatggata agtgtatcac
     4921 ccaccatcat caccatcatt gagaattctg cagatatcca gcacagtggc ggccgctcga
     4981 gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     5041 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     5101 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     5161 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     5221 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     5281 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     5341 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     5401 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     5461 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     5521 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     5581 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     5641 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     5701 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     5761 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     5821 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     5881 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     5941 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
     6001 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     6061 aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg
     6121 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     6181 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     6241 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     6301 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     6361 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     6421 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     6481 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     6541 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     6601 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
     6661 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     6721 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     6781 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     6841 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     6901 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc
     6961 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     7021 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     7081 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     7141 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     7201 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     7261 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     7321 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     7381 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     7441 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     7501 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     7561 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     7621 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     7681 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     7741 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     7801 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     7861 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     7921 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     7981 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     8041 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     8101 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     8161 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     8221 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     8281 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     8341 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     8401 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     8461 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     8521 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     8581 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     8641 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     8701 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     8761 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     8821 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     8881 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     8941 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     9001 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     9061 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     9121 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     9181 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     9241 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     9301 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     9361 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     9421 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
     9481 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
     9541 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
     9601 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgtt
//
