LOCUS       sequence-310436-       11547 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus for doxycycline inducible expression of nontargeting 
            shRNA, mKate marker, based on Addgene 11652.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11547)
  TITLE     Human Citron shRNA and controls
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11547)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Dox nontargeting shRNA mKate
FEATURES             Location/Qualifiers
     source          1..11547
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1299..1532
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1717..1761
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1945..2062
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2131..2342
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2362..2371
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2368..3066
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     misc_feature    3072..3639
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3090..3641
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3234..3251)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3461..3480
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4265..4285
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4388..4582
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     primer_bind     complement(4721..4737)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4722..4738)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    4758..5346
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4811..4831)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5229..5240)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    join(5514..5532,5533..5555,5556..5574,5575..5597,5598..5616,
                     5617..5639,5640..5658,5659..5681,5682..5700,5701..5723,
                     5724..5742,5743..5765,5766..5784)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        5813..6027
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     5948..5967
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    complement(6109..6142)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             6162..6342
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6367..6385)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6368..6385)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6675..6694)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6908..6927)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7027..7049
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7087..7105)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7173..7277
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7278..7346,7347..8138)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7496..7515)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8309..8897
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8798..8817
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9051..9068
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9138..9158)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9143..9472
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9323..9458
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10606..10671
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10801..10821
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11246..11380
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11283..11302)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11337..11356
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccagacc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     1981 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2041 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2101 ccggctcgag tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
     2161 agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa
     2221 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
     2281 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
     2341 aggtgtcgtg acgcgggatc cgccaccatg gtgagcgagc tgattaagga gaacatgcac
     2401 atgaagctgt acatggaggg caccgtgaac aaccaccact tcaagtgcac atccgagggc
     2461 gaaggcaagc cctacgaggg cacccagacc atgagaatca aggcggtcga gggcggccct
     2521 ctccccttcg ccttcgacat cctggctacc agcttcatgt acggcagcaa aaccttcatc
     2581 aaccacaccc agggcatccc cgacttcttt aagcagtcct tccccgaggg cttcacatgg
     2641 gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga caccagcctc
     2701 caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttccc atccaacggc
     2761 cctgtgatgc agaagaaaac actcggctgg gaggcctcca ccgagaccct gtaccccgct
     2821 gacggcggcc tggaaggcag agccgacatg gccctgaagc tcgtgggcgg gggccacctg
     2881 atctgcaact tgaagaccac atacagatcc aagaaacccg ctaagaacct caagatgccc
     2941 ggcgtctact atgtggacag aagactggaa agaatcaagg aggccgacaa agagacctac
     3001 gtcgagcagc acgaggtggc tgtggccaga tactgcgacc tccctagcaa actggggcac
     3061 agatgaggaa ttccgccccc ccccccctaa cgttactggc cgaagccgct tggaataagg
     3121 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
     3181 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
     3241 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
     3301 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
     3361 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
     3421 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
     3481 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
     3541 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aacgtctagg ccccccgaac
     3601 cacggggacg tggttttcct ttgaaaaaca cgatgataat accatggcta gactggacaa
     3661 gagcaaagtc ataaacggcg ctctggaatt actcaatgga gtcggtatcg aaggcctgac
     3721 gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct accctgtact ggcacgtgaa
     3781 gaacaagcgg gccctgctcg atgccctgcc aatcgagatg ctggacaggc atcataccca
     3841 cttctgcccc ctggaaggcg agtcatggca agactttctg cggaacaacg ccaagtcatt
     3901 ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg catctcggca cccgcccaac
     3961 agagaaacag tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc
     4021 cctggagaac gcactgtacg ctctgtccgc cgtgggccac tttacactgg gctgcgtatt
     4081 ggaggaacag gagcatcaag tagcaaaaga ggaaagagag acacctacca ccgattctat
     4141 gcccccactt ctgagacaag caattgagct gttcgaccgg cagggagccg aacctgcctt
     4201 ccttttcggc ctggaactaa tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg
     4261 gtcgccaaaa aagaagagaa aggtcgacgg cggtggtgct ttgtctcctc agcactctgc
     4321 tgtcactcaa ggaagtatca tcaagaacaa ggagggcatg gatgctaagt cactaactgc
     4381 ctggtcccgg acactggtga ccttcaagga tgtatttgtg gacttcacca gggaggagtg
     4441 gaagctgctg gacactgctc agcagatcgt gtacagaaat gtgatgctgg agaactataa
     4501 gaacctggtt tccttgggtt atcagcttac taagccagat gtgatcctcc ggttggagaa
     4561 gggagaagag ccctggctgg tggagagaga aattcaccaa gagacccatc ctgattcaga
     4621 gactgcattt gaaatcaaat catcagttta aggatctcga ctagctagta gctagctagc
     4681 tagtcgagct caagcttcga attcgatatc aagcttatcg cgataccgtc gacctcgacc
     4741 tcgactagtc atatgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     4801 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     4861 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     4921 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     4981 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     5041 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     5101 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     5161 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     5221 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     5281 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     5341 ccccgcatcg gtacgtatgg ccaggtacct ttaagaccaa tgacttacaa ggcagctgta
     5401 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     5461 agacaagatg ggatcaattc accatgctag tggatccccc tcgagtttac cactccctat
     5521 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     5581 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     5641 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     5701 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     5761 accactccct atcagtgata gagaaaagtg aaagtcgggg ctgcaggaat tcgaacgctg
     5821 acgtcatcaa cccgctccaa ggaatcgcgg gcccagtgtc actaggcggg aacacccagc
     5881 gcgcgtgcgc cctggcagga agatggctgt gagggacagg ggagtggcgc cctgcaatat
     5941 ttgcatgtcg ctatgtgttc tgggaaatca ccataaacgt gaaatgtctt tggatttggg
     6001 aatcttataa gttctgtatg agaccacgcg ccctctcgct tgggcgagag taagttcaag
     6061 agacttactc tcgcccaagc gagattttta cgataccgtc gcatgggaat aacttcgtat
     6121 agcatacatt atacgaagtt atgctgcttt ttgcttgtac tgggtctctc tggttagacc
     6181 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     6241 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     6301 gatccctcag acccttttag tcagtgtgga aaatctctag cagcatctag aattaattcc
     6361 gtgtattcta tagtgtcacc taaatcgtat gtgtatgata cataaggtta tgtattaatt
     6421 gtagccgcgt tctaacgaca atatgtacaa gcctaattgt gtagcatctg gcttactgaa
     6481 gcagacccta tcatctctct cgtaaactgc cgtcagagtc ggtttggttg gacgaacctt
     6541 ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc agcgaaccaa tcagcagggt
     6601 catcgctagc cagatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg
     6661 tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt
     6721 cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact
     6781 gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa
     6841 cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gaatggtgca
     6901 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     6961 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     7021 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     7081 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     7141 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     7201 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     7261 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     7321 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     7381 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     7441 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     7501 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     7561 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     7621 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     7681 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     7741 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     7801 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     7861 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     7921 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     7981 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     8041 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     8101 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     8161 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     8221 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     8281 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     8341 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     8401 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     8461 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     8521 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     8581 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     8641 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     8701 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     8761 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     8821 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     8881 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     8941 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     9001 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     9061 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     9121 ttcattaatg cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     9181 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     9241 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     9301 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     9361 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     9421 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttgg
     9481 acacaagaca ggcttgcgag atatgtttga gaataccact ttatcccgcg tcagggagag
     9541 gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg ccagatctct
     9601 ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat aaacaggctg
     9661 ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag atccatgcac
     9721 gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt gccgtaagcc
     9781 gtggcggtct gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac
     9841 cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc
     9901 agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc
     9961 ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc
    10021 tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct ggattgaaca
    10081 gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg
    10141 cctggcactg ccgggcgttg ttctttttaa cttcaggcgg gttacaatag tttccagtaa
    10201 gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc
    10261 gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc
    10321 ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc
    10381 tgatgtggat ctggcgcggc attgacccac gcgaaatcct cgacgtccag gcacgtattg
    10441 tgatgagcga tgccgaacgt accgacgatg atttatacga tacggtgatt ggctaccgtg
    10501 gcggcaactg gatttatgag tgggccccgg atctttgtga aggaacctta cttctgtggt
    10561 gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt
    10621 ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc
    10681 tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc
    10741 tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct
    10801 ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt
    10861 ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag
    10921 gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac ctttataagt
    10981 aggcataaca gttataatca taacatactg ttttttctta ctccacacag gcatagagtg
    11041 tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg
    11101 gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac
    11161 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
    11221 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
    11281 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    11341 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
    11401 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt
    11461 tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg
    11521 ttaaatatgt actacaaact tagtagt
//
