LOCUS       sequence-31045-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  TITLE     The Parkinson Disease-associated Leucine-rich Repeat Kinase 2 
            (LRRK2) Is a Dimer That Undergoes Intramolecular Autophosphorylation
  JOURNAL   J Biol Chem. 2008 Jun 13;283(24):16906-14.
  PUBMED    18397888
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2008-06-13"; volume: "283(24)"; pages: "16906-14"
COMMENT     Sequence Label: 2XMyc-LRRK2-Kinase
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        53..71
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      103..112
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             112..141
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             157..186
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    205..229
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cagagctggt ttagtgaacc gtcagatccg ctagcgatta cgccaagctc gaaattaacc
       61 ctcactaaag ggaacaaaag ctggagctcc accgcggtgg cggccgccat ggagcagaaa
      121 ctcatctctg aagaggatct gagcccgggc ggatccgagc agaaactcat ctctgaagag
      181 gatctgctgc aggaattcga tatcacaagt ttgtacaaaa aagcaggctc cgaattcgcc
      241 cttgacctgc ctagaaatat tatgttgaat aatgatgagt tggaatttga acaagctcca
      301 gagtttctcc taggtgatgg cagttttgga tcagtttacc gagcagccta tgaaggagaa
      361 gaagtggctg tgaagatttt taataaacat acatcactca ggctgttaag acaagagctt
      421 gtggtgcttt gccacctcca ccaccccagt ttgatatctt tgctggcagc tgggattcgt
      481 ccccggatgt tggtgatgga gttagcctcc aagggttcct tggatcgcct gcttcagcag
      541 gacaaagcca gcctcactag aaccctacag cacaggattg cactccacgt agctgatggt
      601 ttgagatacc tccactcagc catgattata taccgagacc tgaaacccca caatgtgctg
      661 cttttcacac tgtatcccaa tgctgccatc attgcaaaga ttgctgacta cggcattgct
      721 cagtactgct gtagaatggg gataaaaaca tcagagggca caccagggtt tcgtgcgcct
      781 gagttgccag aggaaatgtc atttataacc aacaggctga tgtttattca tttggtttac
      841 tactctatga cattttgaca actggaggta gaatagtaga
//
