LOCUS       sequence-31046-e         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  TITLE     The Parkinson Disease-associated Leucine-rich Repeat Kinase 2 
            (LRRK2) Is a Dimer That Undergoes Intramolecular Autophosphorylation
  JOURNAL   J Biol Chem. 2008 Jun 13;283(24):16906-14.
  PUBMED    18397888
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2008-06-13"; volume: "283(24)"; pages: "16906-14"
COMMENT     Sequence Label: 2XMyc-LRRK2-LRRs
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        41..59
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      91..100
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             100..129
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             145..174
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    193..217
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gtgaaccgtc agaatccgct agcgattacg ccaagctcga aattaaccct cactaaaggg
       61 aacaaaagct ggagctccac cgcggtggcg gccgccatgg agcagaaact catctctgaa
      121 gaggatctga gcccgggcgg atccgagcag aaactcatct ctgaagagga tctgctgcag
      181 gaattcgata tcacaagttt gtacaaaaaa gcaggctccg aattcgccct tgactcttct
      241 atggacagtg tgtttgctca aagtgatgac ctggatagtg aaggaagtga aggctcattt
      301 cttgtgaaaa agaaatctaa ttcaattagt gtaggagaat tttaccgaga tgccgtatta
      361 cagcgttgct caccaaattt gcaaagacat tccaattcct tggggcccat ttttgatcat
      421 gaagatttac tgaagcgaaa aagaaaaata ttatcttcag atgattcact caggtcatca
      481 aaacttcaat cccatatgag gcattcagac agcatttctt ctctggcttc tgagagagaa
      541 tatattacat cactagacct ttcagcaaat gaactaagag atattgatgc cctaagccag
      601 aaatgctgta taagtgttca tttggagcat cttgaaaagc tggagcttca ccagaatgca
      661 ctcacgagct ttccacaaca gctatgtgaa actctgaaga gtttgacaca tttggacttg
      721 cacagtaata aatttacatc atttccttct tatttgttga aaatgagttg tattgctaat
      781 cttgatgtct ctcgaaatga cattggaccc tcagtggttt tagatcctac agtgaaatgt
      841 ccaactctga aacagtttaa cctgtcatat aaccagctgt cttttgtacc tgagaacctc
      901 actgatgtgg tagagaaact ggaca
//
