LOCUS       sequence-310479-       17690 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  target Per2 gene with a color-switching luciferase cassette in mouse
            genome and the transfection of the recombinant DNA will lead genomic
            recombination and make a knock-in allele in mouse ES cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17690)
  AUTHORS   Shan Y, Abel JH, Li Y, Izumo M, Cox KH, Jeong B, Yoo SH, Olson DP, 
            Doyle FJ 3rd, Takahashi JS
  TITLE     Dual-Color Single-Cell Imaging of the Suprachiasmatic Nucleus 
            Reveals a Circadian Role in Network Synchrony.
  JOURNAL   Neuron. 2020 Aug 4. pii: S0896-6273(20)30529-8. doi: 
            10.1016/j.neuron.2020.07.012.
  PUBMED    32768389
REFERENCE   2  (bases 1 to 17690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2020 Aug 4. pii: S0896-6273(20)30529-8. doi: 
            10.1016/j.neuron.2020.07.012."
COMMENT     Sequence Label: Per2:iLuc
FEATURES             Location/Qualifiers
     source          1..17690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             260..832
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          910..976
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    1037..1171
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1074..1093)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1128..1147
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1191..1207
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(7485..7518)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             7521..9146
                     /codon_start=1
                     /product="red-emitting variant of click beetle luciferase"
                     /label=CBRluc
                     /note="human codon-optimized"
                     /translation="VKREKNVIYGPEPLHPLEDLTAGEMLFRALRKHSHLPQALVDVVG
                     DESLSYKEFFEATVLLAQSLHNCGYKMNDVVSICAENNTRFFIPVIAAWYIGMIVAPVN
                     ESYIPDELCKVMGISKPQIVFTTKNILNKVLEVQSRTNFIKRIIILDTVENIHGCESLP
                     NFISRYSDGNIANFKPLHFDPVEQVAAILCSSGTTGLPKGVMQTHQNICVRLIHALDPR
                     YGTQLIPGVTVLVYLPFFHAFGFHITLGYFMVGLRVIMFRRFDQEAFLKAIQDYEVRSV
                     INVPSVILFLSKSPLVDKYDLSSLRELCCGAAPLAKEVAEVAAKRLNLPGIRCGFGLTE
                     STSAIIQTLGDEFKSGSLGRVTPLMAAKIADRETGKALGPNQVGELCIKGPMVSKGYVN
                     NVEATKEAIDDDGWLHSGDFGYYDEDEHFYVVDRYKELIKYKGSQVAPAELEEILLKNP
                     CIRDVAVVGIPDLEAGELPSAFVVKQPGKEITAKEVYDYLAERVSHTKYLRGGVRFVDS
                     IPRNVTGKITRKELLKQLLEKAGG"
     RBS             8468..8476
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    9157..9618
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     CDS             complement(9634..10437)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9745..9764)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     10355..10374
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(10451..10947)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     10889..10912
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    complement(10971..11004)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             11007..12632
                     /codon_start=1
                     /product="green-emitting variant of click beetle 
                     luciferase"
                     /label=CBG99luc
                     /note="human codon-optimized"
                     /translation="VKREKNVIYGPEPLHPLEDLTAGEMLFRALRKHSHLPQALVDVVG
                     DESLSYKEFFEATVLLAQSLHNCGYKMNDVVSICAENNTRFFIPVIAAWYIGMIVAPVN
                     ESYIPDELCKVMGISKPQIVFTTKNILNKVLEVQSRTNFIKRIIILDTVENIHGCESLP
                     NFISRYSDGNIANFKPLHFDPVEQVAAILCSSGTTGLPKGVMQTHQNICVRLIHALDPR
                     VGTQLIPGVTVLVYLPFFHAFGFSITLGYFMVGLRVIMFRRFDQEAFLKAIQDYEVRSV
                     INVPSVILFLSKSPLVDKYDLSSLRELCCGAAPLAKEVAEVAAKRLNLPGIRCGFGLTE
                     STSANIHSLGDEFKSGSLGRVTPLMAAKIADRETGKALGPNQVGELCIKGPMVSKGYVN
                     NVEATKEAIDDDGWLHSGDFGYYDEDEHFYVVDRYKELIKYKGSQVAPAELEEILLKNP
                     CIRDVAVVGIPDLEAGELPSAFVVKQPGKEITAKEVYDYLAERVSHTKYLRGGVRFVDS
                     IPRNVTGKITRKELLKQLLEKAGG"
     RBS             11954..11962
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     regulatory      15871..15880
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        15924..16028
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(16029..16097,16098..16889)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(16247..16266)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      17060..17648
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     17549..17568
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggaccgaaca actccgccgc gcgcgctccg tgtaggccgg tgcgggcggc cccgtagcgc
       61 aagggagggc gggaaaggaa gggcgggaca caagggcgaa tctataaagg gcgtcactca
      121 gccagttctc tcctcagaag cgccgagagc gcgaccggga cggttggaga agaaggtggc
      181 tcccggaagg gggagagaca aactgccgta acctctgccg ttcaggatct gcgacctgca
      241 ggtcctcgcc atggaccctg atgatgttgt tgattcttct aaatcttttg tgatggaaaa
      301 cttttcttcg taccacggga ctaaacctgg ttatgtagat tccattcaaa aaggtataca
      361 aaagccaaaa tctggtacac aaggaaatta tgacgatgat tggaaagggt tttatagtac
      421 cgacaataaa tacgacgctg cgggatactc tgtagataat gaaaacccgc tctctggaaa
      481 agctggaggc gtggtcaaag tgacgtatcc aggactgacg aaggttctcg cactaaaagt
      541 ggataatgcc gaaactatta agaaagagtt aggtttaagt ctcactgaac cgttgatgga
      601 gcaagtcgga acggaagagt ttatcaaaag gttcggtgat ggtgcttcgc gtgtagtgct
      661 cagccttccc ttcgctgagg ggagttctag cgttgaatat attaataact gggaacaggc
      721 gaaagcgtta agcgtagaac ttgagattaa ttttgaaacc cgtggaaaac gtggccaaga
      781 tgcgatgtat gagtatatgg ctcaagcctg tgcaggaaat cgtgtcaggc gatctctttg
      841 tgaaggaaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa
      901 agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattcctaat
      961 tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc
     1021 agatccacta ggatctaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     1081 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     1141 actcatcaat gtatcttatc atgtctggat cgtagttcta gagcggaccg gtaaaacgac
     1201 ggccagtgtt aactctagga attcgcagtg cagcccctgc cattcgctgc ccctttggct
     1261 cctgtcatgg ccttcatgct gcccagctac ccgttcccac cagcaacccc aaacctgcct
     1321 caggccttcc tccccagcca gcctcacttt ccagcccacc ccacacttgc ctccgaaata
     1381 actcctgcct cccaggctga gttccctagt cggacctcga cgctcagaca gccgtgcgct
     1441 tgcccagtca cccctccagc cggcacagtg gccctgggca gagcctcccc accgctcttc
     1501 cagtccagag gcagtagtcc cctacaactt aacctgcttc agctagagga ggcgcctgaa
     1561 ggcagcactg gagccgcagg gaccctgggg accacaggga cagcagcttc tggtctggac
     1621 tgcacatctg gcacatctcg ggatcggcag ccaaaggcac ctccaacagt aaggcttctc
     1681 tgctgtgtct ctttctcagg cagtacacat ggtagtcagc acacacccac atgtcagagg
     1741 ggctgctggg tcgagggctt cctgccagcc tccaggcctg agttcagccc gcagtgcctt
     1801 ttctggagtg tgcacttcat agttatgagg tcctaccatg ggacattatt tggagctcta
     1861 tgaggagcaa acgagggatc atgcaaatgg agggaccaag aataatgtgt gcctttggga
     1921 gcatttagga gcctcacatt tctcttcttt tttttttttt cctgtttttt ttttctttta
     1981 gtttttcaag acagggtttc tctgtagaac cttggctatc ttggaactca cagagatctg
     2041 cctgtgtctg cctctcaaat gctggtatta aaggcatgca ccaccactgc ccagatgggc
     2101 ctcacatttc taatgagtct taaaattgtt tttccttttt cttttctctt ttttcttttc
     2161 ttttttcttt tctcttcttc tcttctcttt tcttttcttt ttaaaatttg agacagagtc
     2221 ttactctatc cctgattgtc ctggaatttg ctctgtagac caggttggcc ttaaaatgca
     2281 cagagatctg tctgcctcag ctctaagtgc ggggattaaa agcatgccct tgtgtggcta
     2341 gtctacaaag atgaatggaa cccagcacag ggaaaagggc caggttccag cctgcagtgc
     2401 aggatcctgc ccagtgaagt ccaggctccc ggagctcaca aggatgaaag aggacctccg
     2461 cttttggctc aggagccagt gtgcctgaga gccttaagga tggtgccaag ggcttttctc
     2521 tagtaatggt ttccagaaga actacctata tagtagcagc agcccaagtc agccccgtgc
     2581 cctgtgtgcc ctgtgtgcac tgtgtgccct gtgtgccctg tgtgcactgt gtgccctgtg
     2641 tgcactgtgt gccctgtgtg ccctgtgtgc cctgtgtgca ctgtgtgccc tgtgtgccct
     2701 gtgtgcactg tgtgcactgt gtgccctgtg tgccctgtgt gccctgtgtg ccctgtgtgc
     2761 actgtgtgcc ctgtgtgccc tgtgtgcact gtgtgccctg tgtgccctgt gtgcactgtg
     2821 tgccctgtgt gccctgtgtg cactgtgtgc actatgtgca ctgtgtgccc tgtgtgcact
     2881 gtgtgcacta tgtgcactgt gtgccctgtg tgcactgtgt gccctgtgtg cactgtgtgc
     2941 cctgtgtgca ctgtgtgccc tgtgtgcact gtgtgcactg tgtgccctgt gtgcactgtg
     3001 tgccctgtgt gccctgtgtg ccctgtgtgc cctgtgtgca ctgtgtgccc tgtgtgccct
     3061 gtgtgcactg tgtgcactgt gtgccctgtg tgcactgtgt gcactatgtg cactgtgtgc
     3121 cctgtgtgca ctgtgtgccc tgtgtgcact gtgtgccctg tgtgcactgt gtgccctgtg
     3181 tgccctgtgt gccctgtgtg ccctgtgtgc actgtgtgcc ctgtgtgccc tgtgtgcact
     3241 gtgtgcactg tgtgccctgt gtgcactgtg tgcactatgt gcactgtgtg ccctgtgtgc
     3301 actgtgtgcc ctgtgtgtgt cagcgtacag ggctgagtgc tgccttcaca aggtgtcggc
     3361 gctgctgttc agaacatggg gacgtgagag tgggcagtgc ttctcccgga gcctctctgc
     3421 cctctaagac actaggaccc cgggtgcctc ctaagccaag cagccccact ggggctcaga
     3481 gtctcagtca tgggtggctt actgccccgg aggcagagga gcatcatggc tctttatcca
     3541 ctggagtcca agctatttgc tgtgaccgtc ccaaagtcac attttattgg gtggtattca
     3601 tagggtacca gctactctct ccccataacc acaaaatctg cctgcctgac agacaggtaa
     3661 aggaaaggca tgtgctctat aactgatctt tgcacaaaag ccaccctcgg cacgcccttg
     3721 gttccacctc cccttactgt attttgtatg ctagactatc actgtaagcc ctatggtcag
     3781 tagtgtcttt cctgtcccta aagtgcaacg agccctcaga cactcagaac agtgatgcca
     3841 tctccacgtc aagtgacctg ctcaacctcc ttctgggcga ggacctctgc tcggccactg
     3901 gctcagccct gtctagaagc ggggcatccg ccacctcaga ctctctgggc tccagctcgc
     3961 tgggcttcgg cacatcccaa agtggggcag gtatgtttgt gctgaggaca cagctgtatg
     4021 gtagccagtg tagaagatgc tcatggaata ttctatccct ggctgctctg cctggatgta
     4081 gctccttctg cccgaatcag cagcacagag ggctctagtc atcagaattg ggagtcctag
     4141 ctggtagcgt ccaatttctg tgtcactgaa gaggttacat tcatgccagc ccttgcaggg
     4201 ttagtctgtg ggttccctgg gcattgggca cacatgtgca gacaaaacta acctctaatg
     4261 gaaggaatca cccacatttg tcctagagcc tccagggaca aggccaccct caaaatcctg
     4321 atgccttttc tgccctaaag tgagctttcc ttaacctgct cccaggcctc tctgcccagg
     4381 ggcagcagct gggcacaagt caggccaaca aggccctctt aggacaactc ctctctgggt
     4441 ttctaaaggt ggatggattt gtttccagag tcaacagaga gtgaaaacct gggacaaagg
     4501 tcagggctcc aagaaaggca ctgcctgagt tcaggttcac cccaggcaag agtcacctcc
     4561 tctgtgctag caggtatccc gcgttcgaat cattgagatc attaagactg aagagcaaag
     4621 gggaaggggg gccagcacag gctgtttcta cccaaggaac cccactctcc agagctgcca
     4681 tggctacgca ttagcacatt gtgcaagagt cactctctaa tgtcaaggtt ataacgatcg
     4741 gcgtttcgtg tatttgaagg cagtagtgac acaagtcaca ccagcaaata ctttggaagc
     4801 attgactctt cagagaataa tcacaaagca aaaatgatcc cagacacgga ggaaagcgag
     4861 cagttcatta agtacgtctt gcaggacccc atctggctgc tgatggccaa cacagacgac
     4921 agcatcatga tgacatacca gctgccctcc cggtaactgt tacacttcct gggaagcctg
     4981 gagggctggg taggcgtgtc caacaccaag gtcatccatg caggcaggat accttggaac
     5041 actggctaga cttggcaatg gagagggaag aggcttctcc ttcataaccc cactgggcct
     5101 gcagcagcct gcctactgcc ctgaggcagt tagaacccag gagatcaacc tcttgccagc
     5161 agcacactct tcttttgcca tacattggtg cagtgttgcc ccttgaactg tgttacatca
     5221 cccaccttag gggtaaatgg gatgtgtgtc tgtctgagtc aaaaaaaaaa aaaaaaaaca
     5281 agggtcaaag tgggtgatca gcccatgtct gtggtaaccc tgggcctgcc agagttactc
     5341 agagcccgtc agagtatagg tcaggcgtcc ctgtgaagcc agtgaggtca ctatctttgc
     5401 agaacagccc cagacagggg ctaagagaaa ggaagggcgc cctgaggttc ctgaaggcag
     5461 cagacctctg gtcagtgaca cgggagtaaa ggctggggag tctcagacct ctggtgggag
     5521 acagctgtgc tccaggctgg tgttgacgca ggaaggagac agcatggtcc cagagtctag
     5581 atggttaagt ctcctaggct gaccacacag tgagagccag cccagggctc cagacagcat
     5641 ctgaagatgc ccggctcccc tagctagttt catgaaaaac tgaaattgtg acctaaagaa
     5701 tatttactag agagatgcag ttactttatc tcaaggataa tctactacta tattgttgat
     5761 attgaaaaaa aattgatgaa tgaattatcc ctttggcctg tgacactcac ctatacctaa
     5821 ctcttggtga agggatctcc aggcggtgtt gaaggaggac caggagaagc tgaagctgct
     5881 gcagaggtcc cagccccggt tcacagaggg ccagaggcga gagctccgag aggttcatcc
     5941 gtgggtccac actgggggcc tgcctacggc catcgatgtg acagtaagtt tgcaggacct
     6001 tccttctcac atagcactcc gagattatac ctggggctaa gcatggtagc acacacctgc
     6061 caccccagca cttgggagac tgaggcagga ggactgcagg tctgtgtcta atctggctac
     6121 atagaagatt ctgccacaaa acaagaaaag gttctaggga cctgtcttag tcagggtttc
     6181 tattcctgca caaacatcat gaccaagaag caagttgggg aggaaagggt ttattcggct
     6241 tacacttcta tactgctgtt caccaaggaa gtcaggactg gaactcaagt aggtcagaaa
     6301 gcaggagctg atgcagaggc catggaggga tgttctttac tggcttgctt cccctggctt
     6361 gctcagcctt ctctcttata gaaccaagac taccagccca gagatggtcc cacccacaag
     6421 gggcctttcc ccctcgatca cttattgaga aaatgcctta cagttggatc tcatggaggc
     6481 atttcctcaa ctgaagctcc tttctctggg ataactccag cctgtgtcaa gttgacacaa
     6541 aactagccag tacagtaccc ttaggggtga tcccttccag aggtttaagt ggccggcttg
     6601 aacagcttgc tggcgtccat ggctaccctt tccacccttc tggtttctgt tgagcaacat
     6661 aaacatttct ctgtccacct tgttcttcct gtcatggctc ctgggcctta aaatcccaga
     6721 gccatatatg ctccccaggc ctctgcccat ctgctggctc ataggcgggg gggggggggg
     6781 ggggcgctgc agtccagtgg caacactagc catatccatg ctcttcccag ctcacaggat
     6841 ggaggcccac cttgccaagc tgctaacctt agggccatct agcatgcctc tcctactcca
     6901 tctgttcaca gattacagca ctcaacttta ctaggtagac agccagttcc caccacctcc
     6961 aacacggccc cctgaatgcc caatcttaga agtcatacac ttcccaagtc taagcattct
     7021 ttatggggaa agaagtgaga ctctgcctat tgcaggagga gtggcctttg caaagacttt
     7081 gtggtcatct ggccacctac ccttgtccct cacactcaca taccctcttc acctgagcat
     7141 gtgccatgaa acacttctga tggacatccc tggaaaggga ctcatgacca ggagccagct
     7201 tcctgaggca gggagtgcct ctttggttag caacctccag gtgcatggct aacattcctc
     7261 cctatcctgc tgagtgggaa tgacagagaa gattccagct gcttgcttct tactcatgtg
     7321 acctgttctc tctgaagggc tgtgtttact gcgagagtga ggagaaaggc aacatttgcc
     7381 tgccatatga ggaagacagt ccttccccgg gactctgtga tacctcagaa gccaaagagg
     7441 aggaaggtga acagctgaca ggccccagga tagaggccca gacgataact tcgtatagca
     7501 tacattatac gaagttattg gtaaagcgtg agaaaaatgt catctatggc cctgagcctc
     7561 tccatccttt ggaggatttg actgccggcg aaatgctgtt tcgtgctctc cgcaagcact
     7621 ctcatttgcc tcaagccttg gtcgatgtgg tcggcgatga atctttgagc tacaaggagt
     7681 tttttgaggc aaccgtcttg ctggctcagt ccctccacaa ttgtggctac aagatgaacg
     7741 acgtcgttag tatctgtgct gaaaacaata cccgtttctt cattccagtc atcgccgcat
     7801 ggtatatcgg tatgatcgtg gctccagtca acgagagcta cattcccgac gaactgtgta
     7861 aagtcatggg tatctctaag ccacagattg tcttcaccac taagaatatt ctgaacaaag
     7921 tcctggaagt ccaaagccgc accaacttta ttaagcgtat catcatcttg gacactgtgg
     7981 agaatattca cggttgcgaa tctttgccta atttcatctc tcgctattca gacggcaaca
     8041 tcgcaaactt taaaccactc cacttcgacc ctgtggaaca agttgcagcc attctgtgta
     8101 gcagcggtac tactggactc ccaaagggag tcatgcagac ccatcaaaac atttgcgtgc
     8161 gtctgatcca tgctctcgat ccacgctacg gcactcagct gattcctggt gtcaccgtct
     8221 tggtctactt gcctttcttc catgctttcg gctttcatat tactttgggt tactttatgg
     8281 tcggtctccg cgtgattatg ttccgccgtt ttgatcagga ggctttcttg aaagccatcc
     8341 aagattatga agtccgcagt gtcatcaacg tgcctagcgt gatcctgttt ttgtctaaga
     8401 gcccactcgt ggacaagtac gacttgtctt cactgcgtga attgtgttgc ggtgccgctc
     8461 cactggctaa ggaggtcgct gaagtggccg ccaaacgctt gaatcttcca gggattcgtt
     8521 gtggcttcgg cctcaccgaa tctaccagtg cgattatcca gactctcggg gatgagttta
     8581 agagcggctc tttgggccgt gtcactccac tcatggctgc taagatcgct gatcgcgaaa
     8641 ctggtaaggc tttgggcccg aaccaagtgg gcgagctgtg tatcaaaggc cctatggtga
     8701 gcaagggtta tgtcaataac gttgaagcta ccaaggaggc catcgacgac gacggctggt
     8761 tgcattctgg tgattttgga tattacgacg aagatgagca tttttacgtc gtggatcgtt
     8821 acaaggagct gatcaaatac aagggtagcc aggttgctcc agctgagttg gaggagattc
     8881 tgttgaaaaa tccatgcatt cgcgatgtcg ctgtggtcgg cattcctgat ctggaggccg
     8941 gcgaactgcc ttctgctttc gttgtcaagc agcctggtaa agaaattacc gccaaagaag
     9001 tgtatgatta cctggctgaa cgtgtgagcc atactaagta cttgcgtggc ggcgtgcgtt
     9061 ttgttgactc catccctcgt aacgtaacag gcaaaattac ccgcaaggag ctgttgaaac
     9121 aattgttgga gaaggccggc ggttagccgc ggcagcttct gatggaatta gaacttggca
     9181 aaacaatact gagaatgaag tgtatctgga acagaggctg ctgatctcgt tcttcaggct
     9241 atgaaactga cacatttgga aaccacagta cttagaacca caaagtggga atcaagagaa
     9301 aaacaatgat cccacgagag atctatagat ctatagatca tgagtgggag gaatgagctg
     9361 gcccttaatt tggttttgct tgtttaaatt atgatatcca actatgaaac attatcataa
     9421 agcaatagta aagagccttc agtaaagagc aggcatttat ctaatcccac cccaccccca
     9481 cccccgtagc tccaatcctt ccattcaaaa tgtaggtact ctgttctcac ccttcttaac
     9541 aaagtatgac aggaaaaact tccattttag tggacatctt tattgtttaa tagatcatca
     9601 atttctgcag acttacagga cggatcgatc ccctcagaag aactcgtcaa gaaggcgata
     9661 gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc
     9721 ccattcgccg ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg
     9781 gtccgccaca cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat
     9841 gatattcggc aagcaggcat cgccatgggt cacgacgaga tcatcgccgt cgggcatgcg
     9901 cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc
     9961 atcctgatcg acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc
    10021 ttggtggtcg aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc
    10081 catgatggat actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac
    10141 ttcgcccaat agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca
    10201 aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgca gttcattcag
    10261 ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa
    10321 cacggcggca tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc
    10381 cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt tcaatggccg atcccatatt
    10441 ggctgcaggt cgaaaggccc ggagatgagg aagaggagaa cagcgcggca gacgtgcgct
    10501 tttgaagcgt gcgagaatgc cgggcctcgg gaggaccttc gcgcccgccc cgcccctgag
    10561 cccgcccctg agcccgcccc cggacccacc ccttcccagc ctctgagccc agaaagcgaa
    10621 ggagcaaagc tgctattggc cgctgcccca aaggcctacc cgcttccatt gctcagcggt
    10681 gctgtccatc tgcacgagac tagtgagacg tgctacttcc atttgtcacg tcctgcacga
    10741 cgcgagctgc ggggcggggg ggaacttcct gactagggag gagtagaatg gtggcgcgaa
    10801 ggggccacca aagaacggag ccggttggcg ctaccggtgg atgtggaatg tgtgcgaggc
    10861 cagaggccac ttgtgtagcg ccaagtgcca gcggggctgc taaagcgcat gctccagact
    10921 gccttgggaa aagcgcctcc cctacccggt agaattcact ctagagttag ataacttcgt
    10981 atagcataca ttatacgaag ttattggtga agcgtgagaa aaatgtcatc tatggccctg
    11041 agcctctcca tcctttggag gatttgactg ccggcgaaat gctgtttcgt gctctccgca
    11101 agcactctca tttgcctcaa gccttggtcg atgtggtcgg cgatgaatct ttgagctaca
    11161 aggagttttt tgaggcaacc gtcttgctgg ctcagtccct ccacaattgt ggctacaaga
    11221 tgaacgacgt cgttagtatc tgtgctgaaa acaatacccg tttcttcatt ccagtcatcg
    11281 ccgcatggta tatcggtatg atcgtggctc cagtcaacga gagctacatt cccgacgaac
    11341 tgtgtaaagt catgggtatc tctaagccac agattgtctt caccactaag aatattctga
    11401 acaaagtcct ggaagtccaa agccgcacca actttattaa gcgtatcatc atcttggaca
    11461 ctgtggagaa tattcacggt tgcgaatctt tgcctaattt catctctcgc tattcagacg
    11521 gcaacatcgc aaactttaaa ccactccact tcgaccctgt ggaacaagtt gcagccattc
    11581 tgtgtagcag cggtactact ggactcccaa agggagtcat gcagacccat caaaacattt
    11641 gcgtgcgtct gatccatgct ctcgatccac gcgtgggcac tcagctgatt cctggtgtca
    11701 ccgtcttggt ctacttgcct ttcttccatg ctttcggctt tagcattact ttgggttact
    11761 ttatggtcgg tctccgcgtg attatgttcc gccgttttga tcaggaggct ttcttgaaag
    11821 ccatccaaga ttatgaagtc cgcagtgtca tcaacgtgcc tagcgtgatc ctgtttttgt
    11881 ctaagagccc actcgtggac aagtacgact tgtcttcact gcgtgaattg tgttgcggtg
    11941 ccgctccact ggctaaggag gtcgctgaag tggccgccaa acgcttgaat cttccaggga
    12001 ttcgttgtgg cttcggcctc accgaatcta ccagcgctaa cattcactct ctcggggatg
    12061 agtttaagag cggctctttg ggccgtgtca ctccactcat ggctgctaag atcgctgatc
    12121 gcgaaactgg taaggctttg ggcccgaacc aagtgggcga gctgtgtatc aaaggcccta
    12181 tggtgagcaa gggttatgtc aataacgttg aagctaccaa ggaggccatc gacgacgacg
    12241 gctggttgca ttctggtgat tttggatatt acgacgaaga tgagcatttt tacgtcgtgg
    12301 atcgttacaa ggagctgatc aaatacaagg gtagccaggt tgctccagct gagttggagg
    12361 agattctgtt gaaaaatcca tgcattcgcg atgtcgctgt ggtcggcatt cctgatctgg
    12421 aggccggcga actgccttct gctttcgttg tcaagcagcc tggtaaagaa attaccgcca
    12481 aagaagtgta tgattacctg gctgaacgtg tgagccatac taagtacttg cgtggcggcg
    12541 tgcgttttgt tgactccatc cctcgtaacg taacaggcaa aattacccgc aaggagctgt
    12601 tgaaacaatt gttggagaag gccggcggtt aggatatcaa gcttggggaa gatcttgtgg
    12661 tttctaatca catggaccca tacctacact gctttttttg ttttaggaaa aacaaaaaac
    12721 aaaaacacca tagttttctg gcggtggaac aaaactgagg ggaggtttag gaggaaatcc
    12781 atttttgtat taaaatagaa atacggaatt tgggggatgg ggtgagattc gtcattgaac
    12841 ttgagactga ggtggtctgt gttgtcatgg aggctgcctc atggtcctca ggagtgtctt
    12901 gacctccatg aaacctcttt ccagtgtgcc aatgtcctct ggcccctgtg gattgttctg
    12961 aaacataaca ccaggatgtg gcaggtaaca gggaagccac aagaggctat ccaccaaggg
    13021 cccagctttc tggaactttc tcacagtgtg attgtatctc ccaagcagag agaccatctc
    13081 tcctgacatc ctctcagtgt gttcccttac gtggtttgga gcatggtgta gcagctttgg
    13141 cttcaggtcc tgcctgtggt ggtcaacatt ccagtctgac atggcttctg ttcgtcaaca
    13201 aagttgaaat gcctgctctg gactagtgga gctcagtggc ttctgcaaac gatgcccacc
    13261 atcagactag cacccccaca ctgtacattt ctctgctgtt cttgtatcct ttttagacca
    13321 ttgtggccag tgtgcagaga gagctgtggc atcatcagcc atgttgccgt gtctgcatgg
    13381 tggcctctgc aagccaggct ttgttgctgt agaggacacc gtcacgtgtt tgttctttgg
    13441 ttggactctc tcagacatta gctcccagca gaaagcagcc actgagcacg gaggagagag
    13501 gcacccacac tgctgccctg agttctccag tttgcaggga gctcagcctc ccacttctat
    13561 atgtatatac tcctagctac tgtggcttcg ggtctctgtc acatctatct gtgctgctgg
    13621 tcctcagatc actggaacct gtggagagaa ggggacctct ctgcccagct ctacaaaact
    13681 ttatgctgca tcagacatcc aaagattgtt ccgacatgct tgcgggtgac ccctggtgga
    13741 acgagacatc accagtgagg aatcattgga cttaaaagta gacaaagcct ggagcagagg
    13801 aagctgtttc ctgagtctga agtggctact ggggacatgt cctgctgtag ttggttttca
    13861 tggtaaagcc atctgaggcc tgaatattac ccctattttt cataaacaca agaactctat
    13921 tttttttatt aaagcaacac cacctttcac agtgatcagg tagtagccat gttttaaagg
    13981 aaattcaatg ttacagacag ctgcctctct gaccagtctg atcctaaggg tagatagaag
    14041 atggtctaag cctacgcttg ttacttaaac acaaaactgc caaaaccttc tctcttctct
    14101 cttgaatgtt taccatcagc gttattttat gattatttaa tatatagtcc ttgattgtta
    14161 actgctaaga agttgacttc ctaggataat tttgtgaatc tgtttacaag atgccaagca
    14221 tccagccctg ttttctttag aatgtgtgct tacacgggtg tcctaagaca ttctctattt
    14281 taaactgagc cttcttttta atgtaaataa gctctcagag tttgtgcgat gatgattcgt
    14341 gagccttgcc ggacaagagg tttgttcatg cgcaaaccaa acgtaccttc acccagtgca
    14401 atatatttgt gtgactgctt gtgtcttttt atgacttttt tgccttttag aaaattgtta
    14461 aataaagcaa gtatattttt attttcaact gtatgctttg tttgatgtga tggtggggat
    14521 ttaacccagg acctgatgca aattaagcgt gtgttctaac attgacctat accctagtgc
    14581 tgtatatatt tttaacataa catagaccaa taagccaaaa agaatgacat tgtcaaaaat
    14641 aataaaagaa aaaaacccac acaaacaaca acaacaacaa acctttttaa gaacttcaaa
    14701 atggtacatt aggaaatctc cagttccaga agccagcagc tcttacttcc cttgcactgt
    14761 gggctttgct ggccgatctc ctgtctgtgt atctctcaca tgctcagtct ctctgggcca
    14821 ttcaaaccct gtagggattt cctatgatgc agatctagcc aggcatcccc aggtcccttt
    14881 ctcttggtgt ctctttacat gcacacagtt tagcagattc actaggctgc ccctcagctt
    14941 agcatgaccc ccgcccagca cgcactctcc caataatccc gcctttggct gtttgcctgt
    15001 gtgttggtct cagggctgat tgcagagaaa tcagacaagt accatgctag aaaggaccta
    15061 tataccgtta ggatcagtat ataggcatca ctggggacac aggcctccta cctgaccctt
    15121 cctgattgac agccttgtga gagctactct gaagagacct gcttcccttt cctctctcca
    15181 aaccagggtc ccagggaccg gagcagaggg gcaacatgac actggacctc actcacggtc
    15241 tgcttctggg tgctctgaac ctgccttctg actgcaagta ctggtgcagt ctgacacaaa
    15301 gttctcgtgg caggtaggac tgcttcactg ttcctttgga ggactcttaa ttgaacttta
    15361 cggccagaag gactgcccct ctcctttctc ttcaaaccaa aaggaaattc ttggggtctc
    15421 ctgggcagat tgcccacaaa gcccttggaa gtctgcaggc ctacagctgc ctttgtccct
    15481 ctggggattt acagcctgtt aatttctgct gctaactagt caccctgtgg ccatcatggc
    15541 cttgcctagc ctctggggtt ctctgttcct gcctgggggg gggggggggg attaaaaggc
    15601 cctactacta aggaaatctg catgcaggca aactttgaag gtagggtatg gttcagggga
    15661 caaatggcag gaactagtag tttaactgag ggaggtgaac aggagaaggg tttcctgagc
    15721 ctgagatggg gtgagtagtc acgctgctgg gaccaatgga gtctaagcca cttggctaat
    15781 ggagtcacta aaataaaata aattgatgcc tgcttgtact ttcccttcct caaaggtctt
    15841 aagcttccta gacccggggg ggtcgacgcg gccgccatgg tcatagctgt ttgacgtcag
    15901 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
    15961 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
    16021 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
    16081 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
    16141 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
    16201 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
    16261 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
    16321 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
    16381 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
    16441 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
    16501 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    16561 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    16621 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    16681 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    16741 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    16801 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    16861 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    16921 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    16981 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    17041 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    17101 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    17161 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
    17221 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    17281 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    17341 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    17401 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag cattgagaaa
    17461 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    17521 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    17581 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    17641 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
//
