LOCUS       sequence-310488-         375 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses His-tagged greenFAST in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Tebo AG, Moeyaert B, Thauvin M, Carlon-Andres I, Boken D, Volovitch 
            M, Padilla-Parra S, Dedecker P, Vriz S, Gautier A
  TITLE     Orthogonal fluorescent chemogenetic reporters for multicolor 
            imaging.
  JOURNAL   Nat Chem Biol. 2020 Aug 10. pii: 10.1038/s41589-020-0611-0. doi: 
            10.1038/s41589-020-0611-0.
  PUBMED    32778846
REFERENCE   2  (bases 1 to 375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2020 Aug 10. pii: 10.1038/s41589-020-0611-0. doi: 
            10.1038/s41589-020-0611-0."
COMMENT     Sequence Label: pAG261-His-greenFAST
FEATURES             Location/Qualifiers
     source          1..375
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..375
                     /codon_start=1
                     /product="small protein tag, derived from photoactive 
                     yellow protein, that allows in vivo fluorescent labeling by
                     addition of a cell-permeant fluorogen (Plamont et al., 
                     2016)"
                     /label=Y-FAST
                     /translation="MEHVAFGSEDIENTLAKMDDEQLDGLAFGAIQLDGDGNILQYNAA
                     EGDITGRDPKQVIGKNFFKDVATGTDSPEFYRKFKEGVASGNLNTMFEWMIPTSRGPTK
                     VKVHMKKALSGDSYWVFVKRV"
ORIGIN
        1 atggagcatg ttgcctttgg cagtgaggac atcgagaaca ctctggccaa aatggacgac
       61 gaacaactgg atgggttggc ctttggcgca attcagctcg atggtgacgg gaatatcctg
      121 cagtacaatg ctgctgaagg agacatcaca ggcagagatc ccaaacaggt gattgggaag
      181 aacttcttca aggatgttgc aactggaacg gattctcccg agttttaccg caaattcaag
      241 gaaggcgtag cgtcagggaa tctgaacacc atgttcgaat ggatgatacc gacaagcagg
      301 ggaccaacca aggtcaaggt gcacatgaag aaagcccttt ccggtgacag ctattgggtc
      361 tttgtgaaac gggtg
//
