LOCUS       sequence-31049-e         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  TITLE     The Parkinson Disease-associated Leucine-rich Repeat Kinase 2 
            (LRRK2) Is a Dimer That Undergoes Intramolecular Autophosphorylation
  JOURNAL   J Biol Chem. 2008 Jun 13;283(24):16906-14.
  PUBMED    18397888
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2008-06-13"; volume: "283(24)"; pages: "16906-14"
COMMENT     Sequence Label: AcGFP-LRRK2-WT
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      44..53
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             50..766
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
ORIGIN
        1 agctggttta gtgaaccgtc aggatccgct agcgctaccg gtcgccacca tggtgagcaa
       61 gggcgccgag ctgttcaccg gcatcgtgcc catcctgatc gagctgaatg gcgatgtgaa
      121 tggccacaag ttcagcgtga gcggcgaggg cgagggcgat gccacctacg gcaagctgac
      181 cctgaagttc atctgcacca ccggcaagct gcctgtgccc tggcccaccc tggtgaccac
      241 cctgagctac ggcgtgcagt gcttctcacg ctaccccgat cacatgaagc agcacgactt
      301 cttcaagagc gccatgcctg agggctacat ccaggagcgc accatcttct tcgaggatga
      361 cggcaactac aagtcgcgcg ccgaggtgaa gttcgagggc gataccctgg tgaatcgcat
      421 cgagctgacc ggcaccgatt tcaaggagga tggcaacatc ctgggcaata agatggagta
      481 caactacaac gcccacaatg tgtacatcat gaccgacaag gccaagaatg gcatcaaggt
      541 gaacttcaag atccgccaca acatcgagga tggcagcgtg cagctggccg accactacca
      601 gcagaatacc cccatcggcg atggccctgt gctgctgccc gataaccact acctgtccac
      661 ccagagcgcc ctgtccaagg accccaacga gaagcgcgat cacatgatct acttcggctt
      721 cgtgaccgcc gccgccatca cccacggcat ggatgagctg tacaagtccg gccggactca
      781 gatctcgagc tcaagcttcg aattctgcag tcgacggtac cgc
//
