LOCUS       sequence-310520-       11670 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BAC-based, lacUV5 regulon and Cre recombinase in loxP and lox5171 
            sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11670)
  AUTHORS   Wang G, Zhao Z, Ke J, Engel Y, Shi YM, Robinson D, Bingol K, Zhang 
            Z, Bowen B, Louie K, Wang B, Evans R, Miyamoto Y, Cheng K, Kosina S,
            De Raad M, Silva L, Luhrs A, Lubbe A, Hoyt DW, Francavilla C, Otani 
            H, Deutsch S, Washton NM, Rubin EM, Mouncey NJ, Visel A, Northen T, 
            Cheng JF, Bode HB, Yoshikuni Y
  TITLE     CRAGE enables rapid activation of biosynthetic gene clusters in 
            undomesticated bacteria.
  JOURNAL   Nat Microbiol. 2019 Dec;4(12):2498-2510. doi: 
            10.1038/s41564-019-0573-8. Epub 2019 Oct 14.
  PUBMED    31611640
REFERENCE   2  (bases 1 to 11670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-019-0573-8"; journalName: "Nat Microbiol"; date: 
            "2019-12"; volume: "4"; issue: "12"; pages: "2498-2510"
COMMENT     Sequence Label: pW5Y_Apr
FEATURES             Location/Qualifiers
     source          1..11670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    97..570
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(643..1614)
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     CDS             complement(1614..2789)
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     promoter        3003..3223
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             3224..4027
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     misc_feature    4191..4695
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     misc_feature    4910..5160
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(5163..5918)
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      6009..6228
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     protein_bind    complement(7158..7191)
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     promoter        7215..7305
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     misc_feature    8213..8320
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     8229..8245
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     8230..8246
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8280..8296)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8280..8296)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    8347..8380
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    8555..8575
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(8589..9671)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     9633..9652
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(9672..9749)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     primer_bind     9878..9900
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        join(9957..9962,9963..9980,9981..9987)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    9995..10011
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        join(10052..10057,10058..10075,10076..10082)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    10090..10106
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             10155..11186
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLPVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     oriT            11417..11525
                     /note="incP origin of transfer"
     primer_bind     11570..11588
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11613..11630)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11613..11629)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11622..11644)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 accgaggtta ctccgttcta caggttacga cgacatgtca atacttgccc ttgacaggca
       61 ttgatggaat cgtagtctca cgctgatagt ctgatcgaca atacaagtgg gaccgtggtc
      121 ccagaccgat aatcagaccg acaacacgag tgggatcgtg gtcccagact aataatcaga
      181 ccgacgatac gagtgggacc gtggtcccag actaataatc agaccgacga tacgagtggg
      241 accgtggttc cagactaata atcagaccga cgatacgagt gggaccgtgg tcccagacta
      301 ataatcagac cgacgatacg agtgggacca tggtcccaga ctaataatca gaccgacgat
      361 acgagtggga ccgtggtccc agtctgatta tcagaccgac gatacgagtg ggaccgtggt
      421 cccagactaa taatcagacc gacgatacga gtgggaccgt ggtcccagac taataatcag
      481 accgacgata cgagtgggac cgtggtccca gtctgattat cagaccgacg atacaagtgg
      541 aacagtgggc ccagagagaa tattcaggcc agttatgctt tctggcctgt aacaaaggac
      601 attaagtaaa gacagataaa cgtagactaa aacgtggtcg catcagggtg ctggcttttc
      661 aagttcctta agaatggcct caattttctc tatacactca gttggaacac gagacctgtc
      721 caggttaagc accattttat cgcccttata caatactgtc gctccaggag caaactgatg
      781 tcgtgagctt aaactagttc ttgatgcaga tgacgtttta agcacagaag ttaaaagagt
      841 gataacttct tcagcttcaa atatcacccc agcttttttc tgctcatgaa ggttagatgc
      901 ctgctgctta agtaattcct ctttatctgt aaaggctttt tgaagtgcat cacctgaccg
      961 ggcagatagt tcaccggggt gagaaaaaag agcaacaact gatttaggca atttggcggt
     1021 gttgatacag cgggtaataa tcttacgtga aatattttcc gcatcagcca gcgcagaaat
     1081 atttccagca aattcattct gcaatcggct tgcataacgc tgaccacgtt cataagcact
     1141 tgttgggcga taatcgttac ccaatctgga taatgcagcc atctgctcat catccagctc
     1201 gccaaccaga acacgataat cactttcggt aagtgcagca gctttacgac ggcgactccc
     1261 atcggcaatt tctatgacac cagatactct tcgaccgaac gccggtgtct gttgaccagt
     1321 cagtagaaaa gaagggatga gatcatccag tgcgtcctca gtaagcagct cctggtcacg
     1381 ttcattacct gaccataccc gagaggtctt ctcaacacta tcaccccgga gcacttcaag
     1441 agtaaacttc acatcccgac cacatacagg caaagtaatg gcattaccgc gagccattac
     1501 tcctacgcgc gcaattaacg aatccaccat cggggcagct ggtgtcgata acgaagtatc
     1561 ttcaaccggt tgagtattga gcgtatgttt tggaataaca ggcgcacgct tcattatcta
     1621 atctcccagc gtggtttaat cagacgatcg aaaatttcat tgcagacagg ttcccaaata
     1681 gaaagagcat ttctccaggc accagttgaa gagcgttgat caatggcctg ttcaaaaaca
     1741 gttctcatcc ggatctgacc tttaccaact tcatccgttt cacgtacaac attttttaga
     1801 accatgcttc cccaggcatc ccgaatttgc tcctccatcc acggggactg agagccatta
     1861 ctattgctgt atttggtaag caaaatacgt acatcaggct cgaacccttt aagatcaacg
     1921 ttcttgagca gatcacgaag catatcgaaa aactgcagtg cggaggtgta gtcaaacaac
     1981 tcagcaggcg tgggaacaat cagcacatca gcagcacata cgacattaat cgtgccgata
     2041 cccaggttag gcgcgctgtc aataactatg acatcatagt catgagcaac agtttcaatg
     2101 gccagtcgga gcatcaggtg tggatcggtg ggcagtttac cttcatcaaa tttgcccatt
     2161 aactcagttt caatacggtg cagagccaga caggaaggaa taatgtcaag ccccggccag
     2221 caagtgggct ttattgcata agtgacatcg tccttttccc caagatagaa aggcaggaga
     2281 gtgtcttctg catgaatatg aagatctggt acccatccgt gatacattga ggctgttccc
     2341 tgggggtcgt taccttccac gagcaaaaca cgtagcccct tcagagccag atcctgagca
     2401 agatgaacag aaactgaggt tttgtaaacg ccacctttat gggcagcaac cccgatcacc
     2461 ggtggaaata cgtcttcagc acgtcgcaat cgcgtaccaa acacatcacg catatgatta
     2521 atttgttcaa ttgtataacc aacacgttgc tcaacccgtc ctcgaatttc catatccggg
     2581 tgcggtagtc gccctgcttt ctcggcatct ctgatagcct gagaagaaac cccaactaaa
     2641 tccgctgctt cacctattct ccagcgccgg gttattttcc tcgcttccgg gctgtcatca
     2701 ttaaactgtg caatggcgat agccttcgtc atttcatgac cagcgtttat gcactggtta
     2761 agtgtttcca tgagtttcat tctgaacatc ctttaatcat tgctttgcgt ttttttatta
     2821 aatcttgcaa tttactgcaa agcaacaaca aaatcgcaaa gtcatcaaaa aaccgcaaag
     2881 ttgtttaaaa taagagcaac actacaaaag gagataagaa gagcacatac ctcagtcact
     2941 tattatcact agcgagtgca ccatatttaa atgcccgggc ttaattaagg cgcgcccagc
     3001 ttttcaattc aattcatcat ttttttttta ttcttttttt tgatttcggt ttccttgaaa
     3061 tttttttgat tcggtaatct ccgaacagaa ggaagaacga aggaaggagc acagacttag
     3121 attggtatat atacgcatat gtagtgttga agaaacatga aattgcccag tattcttaac
     3181 ccaactgcac agaacaaaaa cctgcaggaa acgaagataa atcatgtcga aagctacata
     3241 taaggaacgt gctgctactc atcctagtcc tgttgctgcc aagctattta atatcatgca
     3301 cgaaaagcaa acaaacttgt gtgcttcatt ggatgttcgt accaccaagg aattactgga
     3361 gttagttgaa gcattaggtc ccaaaatttg tttactaaaa acacatgtgg atatcttgac
     3421 tgatttttcc atggagggca cagttaagcc gctaaaggca ttatccgcca agtacaattt
     3481 tttactcttc gaagacagaa aatttgctga cattggtaat acagtcaaat tgcagtactc
     3541 tgcgggtgta tacagaatag cagaatgggc agacattacg aatgcacacg gtgtggtggg
     3601 cccaggtatt gttagcggtt tgaagcaggc ggcagaagaa gtaacaaagg aacctagagg
     3661 ccttttgatg ttagcagaat tgtcatgcaa gggctcccta tctactggag aatatactaa
     3721 gggtactgtt gacattgcga agagcgacaa agattttgtt atcggcttta ttgctcaaag
     3781 agacatgggt ggaagagatg aaggttacga ttggttgatt atgacacccg gtgtgggttt
     3841 agatgacaag ggagacgcat tgggtcaaca gtatagaacc gtggatgatg tggtctctac
     3901 aggatctgac attattattg ttggaagagg actatttgca aagggaaggg atgctaaggt
     3961 agagggtgaa cgttacagaa aagcaggctg ggaagcatat ttgagaagat gcggccagca
     4021 aaactaaaaa actgtattat aagtaaatgc atgtatacta aactcacaaa ttagagcttc
     4081 aatttaatta tatcagttat tacccgggaa tctcggtcgt aatgattttt ataatgacga
     4141 aaaaaaaaaa attggaaaga aaaagctggg cgcgccggcc ggcccttttc atcacgtgct
     4201 ataaaaataa ttataattta aattttttaa tataaatata taaattaaaa atagaaagta
     4261 aaaaaagaaa ttaaagaaaa aatagttttt gttttccgaa gatgtaaaag actctagggg
     4321 gatcgccaac aaatactacc ttttatcttg ctcttcctgc tctcaggtat taatgccgaa
     4381 ttgtttcatc ttgtctgtgt agaagaccac acacgaaaat cctgtgattt tacattttac
     4441 ttatcgttaa tcgaatgtat atctatttaa tctgcttttc ttgtctaata aatatatatg
     4501 taaagtacgc tttttgttga aattttttaa acctttgttt attttttttt cttcatttcc
     4561 gtaactcttc taccttcttt atttactttc taaaatccaa atacaaaaca taaaaataaa
     4621 taaacacaga gtaaattccc aaattattcc atcattaaaa gatacgaggc gcgtgtaagt
     4681 tacaggcaag cgatcggccg gcccgggcat ttaaatgcag gccgcgtacg cgtcgacgcg
     4741 taccgaatgc tcgccgcagc cgtgtaaccg agcatagcga gcgaactggc gaggaagcaa
     4801 agaagaactg ttctgtcaga tagctcttac gctcagcgca agaagaaata tccaccgtgg
     4861 gaaaaactcc aggtagaggt acacacgcgg atagccaatt cagagtaata aactgtgata
     4921 atcaaccctc atcaatgatg acgaactaac ccccgatatc aggtcacatg acgaagggaa
     4981 agagaaggaa atcaactgtg acaaactgcc ctcaaatttg gcttccttaa aaattacagt
     5041 tcaaaaagta tgagaaaatc catgcaggct gaaggaaaca gcaaaactgt gacaaattac
     5101 cctcagtagg tcagaacaaa tgtgacgaac caccctcaaa tctgtgacag ataaccctca
     5161 gactatcctg tcgtcatgga agtgatatcg cggaaggaaa atacgatatg agtcgtctgg
     5221 cggcctttct ttttctcaat gtatgagagg cgcattggag ttctgctgtt gatctcatta
     5281 acacagacct gcaggaagcg gcggcggaag tcaggcatac gctggtaact ttgaggcagc
     5341 tggtaacgct ctatgatcca gtcgattttc agagagacga tgcctgagcc atccggctta
     5401 cgatactgac acagggattc gtataaacgc atggcatacg gattggtgat ttcttttgtt
     5461 tcactaagcc gaaactgcgt aaaccggttc tgtaacccga taaagaaggg aatgagatat
     5521 gggttgatat gtacactgta aagccctctg gatggactgt gcgcacgttt gataaaccaa
     5581 ggaaaagatt catagccttt ttcatcgccg gcatcctctt cagggcgata aaaaaccact
     5641 tccttccccg cgaaactctt caatgcctgc cgtatatcct tactggcttc cgcagaggtc
     5701 aatccgaata tttcagcata tttagcaaca tggatctcgc agataccgtc atgttcctgt
     5761 agggtgccat cagattttct gatctggtca acgaacagat acagcatacg tttttgatcc
     5821 cgggagagac tatatgccgc ctcagtgagg tcgtttgact ggacgattcg cgggctattt
     5881 ttacgtttct tgtgattgat aaccgctgtt tccgccatga cagatccatg tgaagtgtga
     5941 caagttttta gattgtcaca ctaaataaaa aagagtcaat aagcagggat aactttgtga
     6001 aaaaacagct tcttctgagg gcaatttgtc acagggttaa gggcaatttg tcacagacag
     6061 gactgtcatt tgagggtgat ttgtcacact gaaagggcaa tttgtcacaa caccttctct
     6121 agaaccagca tggataaagg cctacaaggc gctctaaaaa agaagatcta aaaactataa
     6181 aaaaaataat tataaaaata tccccgtgga taagtggata accccaaggg aagttttttc
     6241 aggcatcgtg tgtaagcaga atatataagt gctgttccct ggtgcttcct cgctcactcg
     6301 agggcttcgc cctgtcgctc gactgcggcg agcactactg gctgtaaaag gacagaccac
     6361 atcatggttc tgtgttcatt aggttgttct gtccattgct gacataatcc gctccacttc
     6421 aacgtaacac cgcacgaaga tttctattgt tcctgaaggc atattcaaat cgttttcgtt
     6481 accgcttgca ggcatcatga cagaacacta cttcctataa acgctacaca ggctcctgag
     6541 attaataatg cggatctcta cgataatggg agattttccc gactgtttcg ttcgcttctc
     6601 agtggataac agccagcttc tctgtttaac agacaaaaac agcatatcca ctcagttcca
     6661 catttccata taaaggccaa ggcatttatt ctcaggataa ttgtttcagc atcgcaaccg
     6721 catcagactc cggcatcgca aactgcaccc ggtgccgggc agccacatcc agcgcaaaaa
     6781 ccttcgtgta gacttccgtt gaactgatgg acttatgtcc catcaggctt tgcagaactt
     6841 tcagcggtat accggcatac agcatgtgca tcgcatagga atggcggaac gtatgtggtg
     6901 tgaccggaac agagaacgtc acaccgtcag cagcagcggc ggcaaccgcc tccccaatcc
     6961 aggtcctgac cgttctgtcc gtcacttccc agatccgcgc tttctctgtc cttcctgtgc
     7021 gacggttacg ccgctccatg agcttatcgc gaataaatac ctgtgacgga agatcacttc
     7081 gcagaataaa taaatcctgg tgtccctgtt gataccggga agccctgggc caacttttgg
     7141 cgaaaatgag acgttgaata acttcgtata gtacacatta tacgaagtta ttcggcacgt
     7201 aagaggttcc aactttgtcg gcacgtaaga ggttccaact ttcaccataa tgaaataaga
     7261 tcactaccgg gcgtattttt tgagttatcg agattttcag gagctatgca atacgaatgg
     7321 cgaaaagccg agctcatcgg tcagcttctc aaccttgggg ttacccccgg cggtgtgctg
     7381 ctggtccaca gctccttccg tagcgtccgg cccctcgaag atgggccact tggactgatc
     7441 gaggccctgc gtgctgcgct gggtccggga gggacgctcg tcatgccctc gtggtcaggt
     7501 ctggacgacg agccgttcga tcctgccacg tcgcccgtta caccggacct tggagttgtc
     7561 tctgacacat tctggcgcct gccaaatgta aagcgcagcg cccatccatt tgcctttgcg
     7621 gcagcggggc cacaggcaga gcagatcatc tctgatccat tgcccctgcc acctcactcg
     7681 cctgcaagcc cggtcgcccg tgtccatgaa ctcgatgggc aggtacttct cctcggcgtg
     7741 ggacacgatg ccaacacgac gctgcatctt gccgagttga tggcaaaggt tccctatggg
     7801 gtgccgagac actgcaccat tcttcaggat ggcaagttgg tacgcgtcga ttatctcgag
     7861 aatgaccact gctgtgagcg ctttgccttg gcggacaggt ggctcaagga gaagagcctt
     7921 cagaaggaag gtccagtcgg tcatgccttt gctcggttga tccgctcccg cgacattgtg
     7981 gcgacagccc tgggtcaact gggccgagat ccgttgatct tcctgcatcc gccagaggcg
     8041 ggatgcgaag aatgcgatgc cgctcgccag tcgattggct gagctcatga gcggagaacg
     8101 agatgacgtt ggaggggcaa ggtcgcgctg attgctgggg caacacgtgg aggcggtgtt
     8161 taaaccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggtaccgg
     8221 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agcccggggg
     8281 atccactagt tctagagcgg ccgccaccgc ggtggagctc actcaaaggc ggtaatacgg
     8341 ttatccataa cttcgtataa tgtatgctat acgaagttat tagcgatgag ctcggacttc
     8401 cattgttcat tccacggaca aaaacagaga aaggaaacga cagaggccaa aaagctcgct
     8461 ttcagcacct gtcgtttcct ttcttttcag ctctgatgcc gcatagttaa gccagtatgg
     8521 tcggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattgattg
     8581 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     8641 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc
     8701 accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc
     8761 aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc
     8821 gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca
     8881 acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca
     8941 accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg
     9001 gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga
     9061 tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac
     9121 agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct
     9181 tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc
     9241 ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta
     9301 atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg
     9361 acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat
     9421 ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca
     9481 atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc
     9541 tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc
     9601 accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt
     9661 actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg
     9721 cgaaaggttt tgcaccattc gatggtgtcg gatacactcc gtctatcgct acgtgactgg
     9781 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     9841 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     9901 ttttcaccgt catcaccgaa acgcgcgagg cagctcactc attaggcacc ccaggcttta
     9961 cactttatgc ttccggctcg tataatgtgt ggaattgtga gcggataaca atttcacaca
    10021 ggaaacagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtataa
    10081 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa caggcctcga ctctagactg
    10141 caggtaccgt tgacatgtcc aatttactgc ccgtacacca aaatttgcct gcattaccgg
    10201 tcgatgcaac gagtgatgag gttcgcaaga acctgatgga catgttcagg gatcgccagg
    10261 cgttttctga gcatacctgg aaaatgcttc tgtccgtttg ccggtcgtgg gcggcatggt
    10321 gcaagttgaa taaccggaaa tggtttcccg cagaacctga agatgttcgc gattatcttc
    10381 tatatcttca ggcgcgcggt ctggcagtaa aaactatcca gcaacatttg ggccagctaa
    10441 acatgcttca tcgtcggtcc gggctgccac gaccaagtga cagcaatgct gtttcactgg
    10501 ttatgcggcg gatccgaaaa gaaaacgttg atgccggtga acgtgcaaaa caggctctag
    10561 cgttcgaacg cactgatttc gaccaggttc gttcactcat ggaaaatagc gatcgctgcc
    10621 aggatatacg taatctggca tttctgggga ttgcttataa caccctgtta cgtatagccg
    10681 aaattgccag gatcagggtt aaagatatct cacgtactga cggtgggaga atgttaatcc
    10741 atattggcag aacgaaaacg ctggttagca ccgcaggtgt agagaaggca cttagcctgg
    10801 gggtaactaa actggtcgag cgatggattt ccgtctctgg tgtagctgat gatccgaata
    10861 actacctgtt ttgccgggtc agaaaaaatg gtgttgccgc gccatctgcc accagccagc
    10921 tatcaactcg cgccctggaa gggatttttg aagcaactca tcgattgatt tacggcgcta
    10981 aggatgactc tggtcagaga tacctggcct ggtctggaca cagtgcccgt gtcggagccg
    11041 cgcgagatat ggcccgcgct ggagtttcaa taccggagat catgcaagct ggtggctgga
    11101 ccaatgtaaa tattgtcatg aactatatcc gtaacctgga tagtgaaaca ggggcaatgg
    11161 tgcgcctgct ggaagatggc gattagtaag aattcccggg ggatccggtg attgattgag
    11221 caagcttggc actggctgat cagctagccc atgggtatgg acagttttcc ctttgatatg
    11281 taacggtcgc tgcataaccc tgcttcgggg tcattatagc gattttttcg gtatatccat
    11341 cctttttcgc acgatataca ggattttgcc aaagggttcg tgtagacttt ccttggtgta
    11401 tccaacggcg tcagccgggc aggataggtg aagtaggccc acccgcgagc gggtgttcct
    11461 tcttcactgt cccttattcg cacctggcgg tgctcaacgg gaatcctgct ctgcgaggct
    11521 ggccgataag ctctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
    11581 cacgaggccc tttcgtcttc aagaattaat tcactggccg tcgttttaca acgtcgtgac
    11641 tgggaaaacc ctggcgttac ccaacacata
//
