LOCUS       sequence-310522-        8578 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LuxCDABE operon (R6K ori) under T7, in loxP and lox5171 sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8578)
  AUTHORS   Wang G, Zhao Z, Ke J, Engel Y, Shi YM, Robinson D, Bingol K, Zhang 
            Z, Bowen B, Louie K, Wang B, Evans R, Miyamoto Y, Cheng K, Kosina S,
            De Raad M, Silva L, Luhrs A, Lubbe A, Hoyt DW, Francavilla C, Otani 
            H, Deutsch S, Washton NM, Rubin EM, Mouncey NJ, Visel A, Northen T, 
            Cheng JF, Bode HB, Yoshikuni Y
  TITLE     CRAGE enables rapid activation of biosynthetic gene clusters in 
            undomesticated bacteria.
  JOURNAL   Nat Microbiol. 2019 Dec;4(12):2498-2510. doi: 
            10.1038/s41564-019-0573-8. Epub 2019 Oct 14.
  PUBMED    31611640
REFERENCE   2  (bases 1 to 8578)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-019-0573-8"; journalName: "Nat Microbiol"; date: 
            "2019-12"; volume: "4"; issue: "12"; pages: "2498-2510"
COMMENT     Sequence Label: pW34
FEATURES             Location/Qualifiers
     source          1..8578
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     42..64
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     56..73
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     57..73
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(98..116)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     oriT            161..269
                     /note="incP origin of transfer"
     rep_origin      425..781
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     protein_bind    complement(824..857)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     907..923
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     907..923
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     973..992
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        973..991
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    992..1016
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1031..1053
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1061..2503
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             2516..3439
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             3488..4570
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             4585..5568
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             5747..6856
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     primer_bind     6954..6974
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(7198..7216)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      7212..7259
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(7348..7364)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7349..7365)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(8229..8331)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    8329..8362
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 aatttttgcg gccgctatgt gttgggtaac gccagggttt tcccagtcac gacgttgtaa
       61 aacgacggcc agtgaattaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
      121 taggttaatg tcatgataat aatggtttct tagagcttat cggccagcct cgcagagcag
      181 gattcccgtt gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa cacccgctcg
      241 cgggtgggcc tacttcacct atcctgcccg gctgacgccg ttggatacac caaggaaagt
      301 ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa aaaggatgga tataccgaaa
      361 aaatcgctat aatgaccccg aagcagggtt atgcagcgaa gatccgcagt tcaacctgtt
      421 gatagtacgt actaagctct catgtttcac gtactaagct ctcatgttta acgtactaag
      481 ctctcatgtt taacgaacta aaccctcatg gctaacgtac taagctctca tggctaacgt
      541 actaagctct catgtttcac gtactaagct ctcatgtttg aacaataaaa ttaatataaa
      601 tcagcaactt aaatagcctc taaggtttta agttttataa gaaaaaaaag aatatataag
      661 gcttttaaag cttttaaggt ttaacggttg tggacaacaa gccagggatg taacgcactg
      721 agaagccctt agagcctctc aaagcaattt tgagtgacac aggaacactt aacggctgac
      781 atgggaatta gccatggcaa agcctcgcaa tccagtgcaa aggataactt cgtatagcat
      841 acattatacg aagttatgat aaccgtatta ccgcctttga gtgagctcca ccgcggtggc
      901 ggccgctcta gaactagtgg atccccatgc gtccggcgta gaggatcgag atcgatctcg
      961 atcccgcgaa attaatacga ctcactatag gggaattgtg agcggataac aattcccctc
     1021 tagaaataat tttgtttaac tttaagaagg agatatacat atgactaaaa aaatttcatt
     1081 cattattaac ggccaggttg aaatctttcc cgaaggtgat gatttagtgc aatccattaa
     1141 ttttggtgat aatagtgttt acctgccaat attgaatgac tctcatgtaa aaaacattat
     1201 tgattgtaat ggaaataacg aattacggtt gcataacatt gtcaattttc tctatacggt
     1261 agggcaaaga tggaaaaatg aagaatactc aagacgcagg acatacattc gtgacttaaa
     1321 aaaatatatg ggatattcag aagaaatggc taagctagag gccaattgga tatctatgat
     1381 tttatgttct aaaggcggcc tttatgatgt tgtagaaaat gaacttggtt ctcgccatat
     1441 catggatgaa tggctacctc aggatgaaag ttatgttcgg gcttttccga aaggtaaatc
     1501 tgtacatctg ttggcaggta atgttccatt atctgggatc atgtctatat tacgcgcaat
     1561 tttaactaag aatcagtgta ttataaaaac atcgtcaacc gatcctttta ccgctaatgc
     1621 attagcgtta agttttattg atgtagaccc taatcatccg ataacgcgct ctttatctgt
     1681 tatatattgg ccccaccaag gtgatacatc actcgcaaaa gaaattatgc aacatgcgga
     1741 tgttattgtc gcttggggag ggccagatgc gattaattgg gcggtagagc atgcgccatc
     1801 ttatgctgat gtgattaaat ttggttctaa aaagagtctt tgcattatcg ataatcctgt
     1861 tgatttgacg tccgcagcga caggtgcggc tcatgatgtt tgtttttacg atcagcgagc
     1921 ttgtttttct gcccaaaaca tatattacat gggaaatcat tatgaggaat ttaagttagc
     1981 gttgatagaa aaacttaatc tatatgcgca tatattaccg aatgccaaaa aagattttga
     2041 tgaaaaggcg gcctattctt tagttcaaaa agaaagcttg tttgctggat taaaagtaga
     2101 ggtggatatt catcaacgtt ggatgattat tgagtcaaat gcaggtgtgg aatttaatca
     2161 accacttggc agatgtgtgt accttcatca cgtcgataat attgagcaaa tattgcctta
     2221 tgttcaaaaa aataagacgc aaaccatatc tatttttcct tgggagtcat catttaaata
     2281 tcgagatgcg ttagcattaa aaggtgcgga aaggattgta gaagcaggaa tgaataacat
     2341 atttcgagtt ggtggatctc atgacggaat gagaccgttg caacgattag tgacatatat
     2401 ttctcatgaa aggccatcta actatacggc taaggatgtt gcggttgaaa tagaacagac
     2461 tcgattcctg gaagaagata agttccttgt atttgtccca taataggtaa aaagtatgga
     2521 aaatgaatca aaatataaaa ccatcgacca cgttatttgt gttgaaggaa ataaaaaaat
     2581 tcatgtttgg gaaacgctgc cagaagaaaa cagcccaaag agaaagaatg ccattattat
     2641 tgcgtctggt tttgcccgca ggatggatca ttttgctggt ctggcggaat atttatcgcg
     2701 gaatggattt catgtgatcc gctatgattc gcttcaccac gttggattga gttcagggac
     2761 aattgatgaa tttacaatgt ctataggaaa gcagagcttg ttagcagtgg ttgattggtt
     2821 aactacacga aaaataaata acttcggtat gttggcttca agcttatctg cgcggatagc
     2881 ttatgcaagc ctatctgaaa tcaatgcttc gtttttaatc accgcagtcg gtgttgttaa
     2941 cttaagatat tctcttgaaa gagctttagg gtttgattat ctcagtctac ccattaatga
     3001 attgccgaat aatctagatt ttgaaggcca taaattgggt gctgaagtct ttgcgagaga
     3061 ttgtcttgat tttggttggg aagatttagc ttctacaatt aataacatga tgtatcttga
     3121 tataccgttt attgctttta ctgcaaataa cgataattgg gtcaagcaag atgaagttat
     3181 cacattgtta tcaaatattc gtagtaatcg atgcaagata tattctttgt taggaagttc
     3241 gcatgacttg agtgaaaatt tagtggtcct gcgcaatttt tatcaatcgg ttacgaaagc
     3301 cgctatcgcg atggataatg atcatctgga tattgatgtt gatattactg aaccgtcatt
     3361 tgaacattta actattgcga cagtcaatga acgccgaatg agaattgaga ttgaaaatca
     3421 agcaatttct ctgtcttaaa atctattgag atattctatc actcaaatag caatataagg
     3481 actctctatg aaatttggaa actttttgct tacataccaa cctccccaat tttctcaaac
     3541 agaggtaatg aaacgtttgg ttaaattagg tcgcatctct gaggagtgtg gttttgatac
     3601 cgtatggtta ctggagcatc atttcacgga gtttggtttg cttggtaacc cttatgtcgc
     3661 tgctgcatat ttacttggcg cgactaaaaa attgaatgta ggaactgccg ctattgttct
     3721 tcccacagcc catccagtac gccaacttga agatgtgaat ttattggatc aaatgtcaaa
     3781 aggacgattt cggtttggta tttgccgagg gctttacaac aaggactttc gcgtattcgg
     3841 cacagatatg aataacagtc gcgccttagc ggaatgctgg tacgggctga taaagaatgg
     3901 catgacagag ggatatatgg aagctgataa tgaacatatc aagttccata aggtaaaagt
     3961 aaaccccgcg gcgtatagca gaggtggcgc accggtttat gtggtggctg aatcagcttc
     4021 gacgactgag tgggctgctc aatttggcct accgatgata ttaagttgga ttataaatac
     4081 taacgaaaag aaagcacaac ttgagcttta taatgaagtg gctcaagaat atgggcacga
     4141 tattcataat atcgaccatt gcttatcata tataacatct gtagatcatg actcaattaa
     4201 agcgaaagag atttgccgga aatttctggg gcattggtat gattcttatg tgaatgctac
     4261 gactattttt gatgattcag accaaacaag aggttatgat ttcaataaag ggcagtggcg
     4321 tgactttgta ttaaaaggac ataaagatac taatcgccgt attgattaca gttacgaaat
     4381 caatcccgtg ggaacgccgc aggaatgtat tgacataatt caaaaagaca ttgatgctac
     4441 aggaatatca aatatttgtt gtggatttga agctaatgga acagtagacg aaattattgc
     4501 ttccatgaag ctcttccagt ctgatgtcat gccatttctt aaagaaaaac aacgttcgct
     4561 attatattag ctaaggagaa agaaatgaaa tttggattgt tcttccttaa cttcatcaat
     4621 tcaacaactg ttcaagaaca aagtatagtt cgcatgcagg aaataacgga gtatgttgat
     4681 aagttgaatt ttgaacagat tttagtgtat gaaaatcatt tttcagataa tggtgttgtc
     4741 ggcgctcctc tgactgtttc tggttttctg ctcggtttaa cagagaaaat taaaattggt
     4801 tcattaaatc acatcattac aactcatcat cctgtccgca tagcggagga agcttgctta
     4861 ttggatcagt taagtgaagg gagatttatt ttagggttta gtgattgcga aaaaaaagat
     4921 gaaatgcatt tttttaatcg cccggttgaa tatcaacagc aactatttga agagtgttat
     4981 gaaatcatta acgatgcttt aacaacaggc tattgtaatc cagataacga tttttatagc
     5041 ttccctaaaa tatctgtaaa tccccatgct tatacgccag gcggacctcg gaaatatgta
     5101 acagcaacca gtcatcatat tgttgagtgg gcggccaaaa aaggtattcc tctcatcttt
     5161 aagtgggatg attctaatga tgttagatat gaatatgctg aaagatataa agccgttgcg
     5221 gataaatatg acgttgacct atcagagata gaccatcagt taatgatatt agttaactat
     5281 aacgaagata gtaataaagc taaacaagag acgcgtgcat ttattagtga ttatgttctt
     5341 gaaatgcacc ctaatgaaaa tttcgaaaat aaacttgaag aaataattgc agaaaacgct
     5401 gtcggaaatt atacggagtg tataactgcg gctaagttgg caattgaaaa gtgtggtgcg
     5461 aaaagtgtat tgctgtcctt tgaaccaatg aatgatttga tgagccaaaa aaatgtaatc
     5521 aatattgttg atgataatat taagaagtac cacatggaat atacctaata gatttcgagt
     5581 tgcagcgagg cggcaagtga acgaatcccc aggagcatag ataactatgt gactggggtg
     5641 agtgaaagca gccaacaaag cagcagcttg aaagatgaag ggtataaaag agtatgacag
     5701 cagtgctgcc atactttcta atattatctt gaggagtaaa acaggtatga cttcatatgt
     5761 tgataaacaa gaaattacag caagctcaga aattgatgat ttgatttttt cgagcgatcc
     5821 attagtgtgg tcttacgacg agcaggaaaa aatcagaaag aaacttgtgc ttgatgcatt
     5881 tcgtaatcat tataaacatt gtcgagaata tcgtcactac tgtcaggcac acaaagtaga
     5941 tgacaatatt acggaaattg atgacatacc tgtattccca acatcggttt ttaagtttac
     6001 tcgcttatta acttctcagg aaaacgagat tgaaagttgg tttaccagta gcggcacgaa
     6061 tggtttaaaa agtcaggtgg cgcgtgacag attaagtatt gagagactct taggctctgt
     6121 gagttatggc atgaaatatg ttggtagttg gtttgatcat caaatagaat tagtcaattt
     6181 gggaccagat agatttaatg ctcataatat ttggtttaaa tatgttatga gtttggtgga
     6241 attgttatat cctacgacat ttaccgtaac agaagaacga atagattttg ttaaaacatt
     6301 gaatagtctt gaacgaataa aaaatcaagg gaaagatctt tgtcttattg gttcgccata
     6361 ctttatttat ttactctgcc attatatgaa agataaaaaa atctcatttt ctggagataa
     6421 aagcctttat atcataaccg gaggcggctg gaaaagttac gaaaaagaat ctctgaaacg
     6481 tgatgatttc aatcatcttt tatttgatac tttcaatctc agtgatatta gtcagatccg
     6541 agatatattt aatcaagttg aactcaacac ttgtttcttt gaggatgaaa tgcagcgtaa
     6601 acatgttccg ccgtgggtat atgcgcgagc gcttgatcct gaaacgttga aacctgtacc
     6661 tgatggaacg ccggggttga tgagttatat ggatgcgtca gcaaccagtt atccagcatt
     6721 tattgttacc gatgatgtcg ggataattag cagagaatat ggtaagtatc ccggcgtgct
     6781 cgttgaaatt ttacgtcgcg tcaatacgag gacgcagaaa gggtgtgctt taagcttaac
     6841 cgaagcgttt gatagttgca ggcatgagct tgacggccgc gggccccatg gatcgatagc
     6901 tggtcgacac aatctgccct ttcgaaagat cccaacgaaa agcgtgacca catggtcctt
     6961 cttgagtttg taactgctgc tgggattaca catggcatgg atgagctcta caaataatga
     7021 gctagcccgc ctaatgagcg ggcttttttt tctcggccta ggagatactt aacagggaag
     7081 tgagagggcc gcggcaaagc cgtttttcca taggctccgc ccccctgaca agcatcacga
     7141 aatctgacgc tcaaatcagt ggcaaagccc gaaaggaagc tgagttggct gctgccaccg
     7201 ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc
     7261 tgaaaggagg aactatatcc ggattggcga atgggacgcg ccctgtagcg gcgcattaag
     7321 gctgcaggaa ttcgatatca agcttatcga taccgtcgac ctcgaggggg ggcccggtac
     7381 cctccacgtg ttgccccagc aatcagcgcg accttgcccc tccaacgtca tctcgttctc
     7441 cgctcatgag ctcagccaat cgactggcga gcggcatcgc attcttcgca tcccgcctct
     7501 ggcggatgca ggaagatcaa cggatctcgg cccagttgac ccagggctgt cgccacaatg
     7561 tcgcgggagc ggatcaaccg agcaaaggca tgaccgactg gaccttcctt ctgaaggctc
     7621 ttctccttga gccacctgtc cgccaaggca aagcgctcac agcagtggtc attctcgaga
     7681 taatcgacgc gtaccaactt gccatcctga agaatggtgc agtgtctcgg caccccatag
     7741 ggaacctttg ccatcaactc ggcaagatgc agcgtcgtgt tggcatcgtg tcccacgccg
     7801 aggagaagta cctgcccatc gagttcatgg acacgggcga ccgggcttgc aggcgagtga
     7861 ggtggcaggg gcaatggatc agagatgatc tgctctgcct gtggccccgc tgccgcaaag
     7921 gcaaatggat gggcgctgcg ctttacattt ggcaggcgcc agaatgtgtc agagacaact
     7981 ccaaggtccg gtgtaacggg cgacgtggca ggatcgaacg gctcgtcgtc cagacctgac
     8041 cacgagggca tgacgagcgt ccctcccgga cccagcgcag cacgcagggc ctcgatcagt
     8101 ccaagtggcc catcttcgag gggccggacg ctacggaagg agctgtggac cagcagcaca
     8161 ccgccggggg taaccccaag gttgagaagc tgaccgatga gctcggcttt tcgccattcg
     8221 tattgcattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     8281 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgaat aacttcgtat
     8341 aatgtgtact atacgaagtt attcaacgtc tcattttcgc caaaagttgg cccagggctt
     8401 cccggtatca acagggacac caggatttat ttattctgcg aagtgatctt ccgtcacagg
     8461 tatttattcg cgataagctc atggagcggc gtaaccgtcg cacaggaagg acagagaaag
     8521 cgcggatctg ggaagtgacg gacagaacgg tcaggacctg gagcggccgc ttttttcc
//
