LOCUS       sequence-310530-        5042 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains a phzS-phzM operon BioBrick.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5042)
  AUTHORS   Clifford ER, Bradley RW, Wey LT, Lawrence JM, Chen X, Howe CJ, Zhang
            JZ
  TITLE     Phenazines as model low-midpoint potential electron shuttles for 
            photosynthetic bioelectrochemical systems.
  JOURNAL   Chem Sci. 2021 Jan 15;12(9):3328-3338. doi: 10.1039/d0sc05655c.
  PUBMED    34164103
REFERENCE   2  (bases 1 to 5042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Chem Sci"; date: "2021-01-15- 15"; volume: "12"; issue: "9"; pages:
            "3328-3338"
COMMENT     Sequence Label: pSB3C5-phzSM
FEATURES             Location/Qualifiers
     source          1..5042
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..22
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    2327..2347
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      2383..2414
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     rep_origin      2925..3470
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     3371..3390
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3587..3604
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             4178..4837
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4244..4263)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      complement(4967..4996)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 gaattcgcgg ccgcttctag agccatcgat tcgaacactc gagaaaagga agcacccatg
       61 agcgaaccca tcgatatcct catcgccggc gccggcatcg gcggcctcag ttgcgccctg
      121 gccctgcacc aggccggcat cggcaaggtc acgctgctgg aaagcagcag cgagatacgc
      181 ccccttggcg tcggcatcaa tatccagccg gcggcggtcg aggcccttgc cgaactgggc
      241 ctcggcccgg cgctggcggc caccgccatc cccacccacg agctgcgcta catcgaccag
      301 agcggcgcca cggtatggtc cgagccgcgc ggggtggaag ccggcaacgc ctatccgcag
      361 tactcgatcc atcgcggcga attgcagatg atcctgctcg ccgcggtgcg cgagcgtctc
      421 ggccaacagg cggtacgcac cggtctcggc gtggagcgca tcgaagagcg cgacggccgc
      481 gtactgatcg gcgcccgcga cggacacggc aagcccctgg cgctcggtgc cgatgtgctg
      541 gtcggcgccg acggcatcca ttcggcggtc cgcgcgcacc tgcatcccga ccaggggccg
      601 ctgtcccacg gtgggatcac catgtggcgc ggcgtcaccg agttcgaccg cttcctcgac
      661 ggcaagacca tgatcgtcgc caacgacgag cactggtcgc gcctggtcgc ctatccgatc
      721 tcggcgcgcc acgcggccga aggcaagtcg ctggtgaact gggtgtgcat ggtgccgagc
      781 gccgccgtcg gccagctcga caacgaggcc gactggaacc gcaacggacg cctggaagac
      841 gtgttgccgt tcttcgccga ctgggacctg ggctggttcg acatccgcga cctgctgacc
      901 cgcaaccagt tgatcttgca gtacccgatg gtcgaccgcg atccgctgcc gcactggggc
      961 cggggacgca tcacgctgct cggcgacgcc gcccacctga tgtatccgat gggcgccaac
     1021 ggcgcttcgc aggcgatcct cgacggcatc gagctggccg ccgcgctggc gcgcaacgcc
     1081 gacgtggccg cagccctgcg cgaatacgaa gaagcgcggc ggccgaccgc caacaagatc
     1141 atcctggcca accgcgaacg ggaaaaagag gaatgggccg cggcttcgcg accgaagact
     1201 gagaagagcg cggcgctgga agcgatcacc ggcagctacc gcaaccaggt ggaacggcca
     1261 cgctaatact agagtgggta cttctcgggt tacggaaatg tgtaacgaga gagaataaaa
     1321 gatgaataat tcgaatcttg ctgctgcgcg taatttgata caagttgtta ccggggaatg
     1381 gaagtcccgt tgcgtctacg tcgctacgcg cctcgggctg gccgatctga tcgagagcgg
     1441 gatcgacagc gacgagacgc tggccgccgc ggtcggttcc gatgccgagc gcatccatcg
     1501 actgatgcgc ctgctggtgg ccttcgagat cttccagggc gatacccgcg acggctacgc
     1561 taataccccc accagccacc tgctgaggga tgtcgagggc tccttccgcg acatggtgct
     1621 gttctacggc gaggagttcc acgccgcctg gacgcccgcc tgcgaggcgc tgctcagcgg
     1681 taccccaggc ttcgaactgg cgttcggcga agacttctac agctacctga agcgctgccc
     1741 ggatgccggc cggcgcttcc tgctggcgat gaaggcgagc aacctggcct tccacgagat
     1801 ccccaggctc ctggatttcc gcgggcgtag cttcgtcgac gtcggtggcg gttccggcga
     1861 attgaccaag gccatcctgc aagccgagcc cagcgcccgg ggcgtgatgc tcgatcgcga
     1921 gggttccctc ggcgtggccc gcgacaacct ctccagcctg ttggcagggg agcgcgtcag
     1981 cctggtgggc ggcgacatgc tgcaagaggt gccgtccaac ggcgatatct acctgctgtc
     2041 gcggatcatc ggcgatctgg acgaagccgc cagcctgagg ttgctcggca attgccgcga
     2101 ggcgatggcc ggcgacggcc gggtggtggt gatcgagcgg accatctcgg ccagcgagcc
     2161 gtcgccgatg tcggtgctct gggacgtgca cctgttcatg gcctgcgctg gccgccaccg
     2221 caccaccgag gaggtggtcg acctgctcgg gcgcggcggc ttcgcggtgg agcggatcgt
     2281 cgacctgccg atggaaaccc gcatgatcgt cgctgccagg gcctaatact agtagcggcc
     2341 gctgcaggag tcactaaggg ttagttagtt agattagcag aaagtcaaaa gcctccgacc
     2401 ggaggctttt gactaaaact tcccttgggg ttatcattgg ggctcactca aaggcggtaa
     2461 tcagataaaa aaaatcctta gctttcgcta aggatgattt ctgctagaga tggaatagac
     2521 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     2581 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     2641 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     2701 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     2761 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     2821 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     2881 ttttcgttcc actgagcgtc agacccctta ataagatgat cttcttgaga tcgttttggt
     2941 ctgcgcgtaa tctcttgctc tgaaaacgaa aaaactgcct tgcagggcgg tttttcgaag
     3001 gttctctgag ctaccaactc tttgaaccga ggtaactggc ttggaggagc gcagtcacca
     3061 aaacttgtcc tttcagttta gccttaaccg gcgcatgact tcaagactaa ctcctctaaa
     3121 tcaattacca gtggctgctg ccagtggtgc ttttgcatgt ctttccgggt tggactcaag
     3181 acgatagtta ccggataagg cgcagcggtc ggactgaacg gggggttcgt gcatacagtc
     3241 cagcttggag cgaactgcct acccggaact gagtgccagg cgtggaatga gacaaacgcg
     3301 gccataacag cggaatgaca ccggtaaacc gaaaggcagg aacaggagag cgcacgaggg
     3361 agccgccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc caccactgat
     3421 ttgagcgtca gatttcgtga tgcttgtcag gggggcggag cctatggaaa aacggctttg
     3481 ccgcggccct ctcacttccc tgttaagtat cttcctggca tcttccagga aatctccgcc
     3541 tcgttcgtaa gccatttccg ctcgccgcag tcgaacgacc gagcgtagcg agtcagtgag
     3601 cgaggaagcg gaatatatcc tgtatcacat attctgctga cgcaccggtg cagccttttt
     3661 tctcctgcca catgaagcac ttcactgaca ccctcatcag tgccaacata gtaagccagt
     3721 atacactccg ctagcgctga ggtctgcctc gtgaagaagg tgttgctgac tcataccagg
     3781 cctgaatcgc cccatcatcc agccagaaag tgagggagcc acggttgatg agagctttgt
     3841 tgtaggtgga ccagttggtg attttgaact tttgctttgc cacggaacgg tctgcgttgt
     3901 cgggaagatg cgtgatctga tccttcaact cagcaaaagt tcgatttatt caacaaagcc
     3961 acgttgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa
     4021 caataaaact gtctgcttac ataaacagta atacaggggt gtttactaga ggttgatcgg
     4081 gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt
     4141 tgagttatcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcacgggata
     4201 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt
     4261 tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt
     4321 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa
     4381 cgctcacccg gagtttcgta tggccatgaa agacggtgag ctggtgatct gggatagtgt
     4441 tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcgtccc tctggagtga
     4501 ataccacgac gatttccggc agtttctcca catatattcg caagatgtgg cgtgttacgg
     4561 tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgttttttg tctcagccaa
     4621 tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc
     4681 ccccgttttc acgatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc
     4741 gatccaggtt catcatgccg tttgtgatgg cttccatgtc ggccgcatgc ttaatgaatt
     4801 acaacagtac tgtgatgagt ggcagggcgg ggcgtaataa tactagctcc ggcaaaaaaa
     4861 cgggcaaggt gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta cttcgcgttt
     4921 gccacctgac gtctaagaaa aggaatattc agcaatttgc ccgtgccgaa gaaaggccca
     4981 cccgtgaagg tgagccagtg agttgattgc tacgtaatta gttagttagc ccttagtgac
     5041 tc
//
