LOCUS       sequence-310532-        8976 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains a seven gene phzABCDEFG operon BioBrick.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8976)
  AUTHORS   Clifford ER, Bradley RW, Wey LT, Lawrence JM, Chen X, Howe CJ, Zhang
            JZ
  TITLE     Phenazines as model low-midpoint potential electron shuttles for 
            photosynthetic bioelectrochemical systems.
  JOURNAL   Chem Sci. 2021 Jan 15;12(9):3328-3338. doi: 10.1039/d0sc05655c.
  PUBMED    34164103
REFERENCE   2  (bases 1 to 8976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Chem Sci"; date: "2021-01-15- 15"; volume: "12"; issue: "9"; pages:
            "3328-3338"
COMMENT     Sequence Label: pUC19-phzAG
FEATURES             Location/Qualifiers
     source          1..8976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..22
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    6315..6335
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(6360..6376)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6360..6376)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6373..6395)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6384..6400
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6408..6414,6415..6432,6433..6438))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6453..6474
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6591..6608)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6762..7350)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6842..6861)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7521..8312,8313..8381))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8144..8163
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8382..8486)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8554..8572
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8610..8632)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8732..8751
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8945..8967
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8960..8976
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattcgcgg ccgcttctag aggagccctc tcggaggcgg cgcatgaacg gtcagcggta
       61 cagggaaaca cccctcgaca tcgagcgtct gcggcgcctg aatcgcgcca cggtggagcg
      121 ctacatggca atgaaggggg ccgaacggtt acagcggcac agcctgttcg tcgaggacgg
      181 ctgcgccggc aactggacca cggaaagcgg cgaacccctg gttttccggg gccatgagag
      241 cctcaggcgg ctcgccgagt ggctcgagcg ctgcttcccc gactgggagt ggcacaacgt
      301 gcggatcttc gagaccgagg atccgaacca cttctgggtc gagtgcgacg ggcgcggcaa
      361 ggcgctggtc ccggggtatc cgcagggcta ttgcgagaac cactacatcc attccttcga
      421 actcgagaac ggccggataa aacgtaatcg cgagttcatg aacccgatac agaaactgcg
      481 tgcattggga atagccgttc cgcaaataaa acgtgacggt attcccactt gaatgacatc
      541 taccgccaag gagcattgac gatgcctgat acgacaaatc caatcggttt caccgatgcc
      601 aacgaacttc gcgaaaagaa tcgcgccacc gtcgagaagt acatgaatac caaaggccag
      661 gatcgcctgc gccgccatga acttttcgtc gaggacggct gtggcggttt atggaccacc
      721 gataccggct cgcccatcgt cattcgtggc aaagacaaac ttgcggaaca tgccgtctgg
      781 tcattgaagt gctttcccga ttgggagtgg tacaacataa atatattcgg aaccgacgac
      841 cccaatcatt tctgggtcga atgcgacggt cacggcaaga ttcttttccc cggatatccc
      901 gaaggatatt acgaaaacca cttcctgcat tccttcgaac ttgaagacgg caagatcaag
      961 cgcaaccgcg aatttatgaa cgtcttccag caattgcgcg ccttgagcat tccggtcccg
     1021 cagatcaaac gcgaaggcat tcccacctga ggccatcctg gaaggggtga actatggacg
     1081 atctattgca acgcgtacgg cgctgcgaag cgttgcagca acccgaatgg ggcgatccgt
     1141 cgcgcctgcg cgacgtgcag gcgtacctgc gcggcagtcc ggcgctgatc cgcgccggcg
     1201 acatcctggc cctgcgcgcg accctggcgc gggtcgcccg cggcgaggcg ctggtggtgc
     1261 agtgcggcga ctgcgccgag gacatggacg accaccatgc cgagaacgtg gcgcgcaagg
     1321 ccgccgtgct ggaactgctg gccggcgccc tgcgcctggc cggccggcgg ccggtgatcc
     1381 gcgtcgggcg catcgccggg cagtacgcca agccgcgttc caagccgcac gagcaggtcg
     1441 gcgagcagac cctgccggtc tatcgcggcg acatggtcaa cggccgcgag gcccatgccg
     1501 aacagcgccg ggccgatccg cagcggatcc tcaagggcta tgcggcggcg cgcaacatca
     1561 tgcgccacct gggctgggac gccgcgtccg ggcaggaggc gaatgcctcg ccggtctgga
     1621 ccagccacga gatgctgctg ctcgactacg agctgtcgat gctgcgcgag gacgagcagc
     1681 gccgggtcta tctcggttcg acccactggc cgtggatcgg cgagcgcacc cgccaggtcg
     1741 acggcgccca tgtggcgctg ctggccgagg tgctcaaccc ggtggcctgc aaggtcggtc
     1801 cggagatcgg ccgcgaccag ttgctggcgc tctgcgagcg cctcgatccg cgccgcgagc
     1861 cgggacgcct gacgctgatc gcgcggatgg gcgcgcagaa ggtcggcgag cgcctgccgc
     1921 cgctggtgga ggcggtgcgc gcggccgggc acccggtgat ctggctgagc gacccgatgc
     1981 acggcaacac catcgtcgcg ccctgcggca acaagacccg cctggtgcgc agcatcgccg
     2041 aggaggtggc ggcgttccgc ctggcggtgt ccggctccgg cggcgtggcc gccgggctgc
     2101 acctggaaac caccccggac gacgtcaccg agtgcgtcgc cgattccagc ggcctgcacc
     2161 aggtcagccg gcactacacc agcctctgcg atccgcggct gaacccctgg caggcgctca
     2221 gcgcggtgat ggcctggtcc ggcgcagaag cgatcccgag cgcaaccttc cccctggaga
     2281 ccgtggcatg agcggcattc ccgaaatcac cgcctatccc ttgccgaccg cccagcaact
     2341 gccggccaac ctggcgcgct ggagcctgga gccgcggcgc gcggtgctgc tggtgcacga
     2401 catgcagcgc tacttcctac gcccgctgcc cgagagcctg cgcgccggac tggtggccaa
     2461 cgccgcgcgg ctgcgccgct ggtgcgtcga gcagggcgtg cagatcgcct acaccgccca
     2521 gccgggcagc atgaccgagg agcagcgcgg tctgctcaag gacttctggg ggccgggcat
     2581 gcgcgccagt ccggccgacc gcgaggtggt cgaggagctg gcgccggggc cggacgactg
     2641 gctgctgacc aagtggcgct acagcgcctt cttccactcc gacctgctcc agcggatgcg
     2701 cgccgccggc cgcgaccagt tggtgctgtg cggcgtatac gcccacgtcg gggtgctgat
     2761 ctccacggtc gacgcctact ccaacgacat ccagccgttc ctggtggccg acgccatcgc
     2821 cgacttcagc gaggcgcacc accgcatggc cctggaatac gccgccagcc gctgcgcgat
     2881 ggtggtcacc accgacgagg tgctggaatg aacgccctgc ccacatcgct gttgcagcgc
     2941 ctgctcgaac ggccggcgcc gttcgccttg ctctaccgcc cggaaagcaa tggcccgggc
     3001 ctgctcgacg tgatccgcgg cgaggcgctc gaactgcacg gcctggccga cctgccgctg
     3061 gacgagcccg gcccgggcct gccgcgccat gacctgctgg cgctgattcc ctaccggcag
     3121 atcgccgagc gcggcttcga ggcgctcgac gacggcacgc cgctgctggc gctgaaggtc
     3181 ctggagcagg aactgctgcc gctggagcag gccctggcgc tgctgccgaa ccaggcgctg
     3241 gaactgagcg aggaaggttt cgacctcgac gacgaggcct acgccgaagt ggtcgggcgg
     3301 gtcatcgccg acgagatcgg tcgcggcgaa ggcgccaact tcgtgatcaa gcggcgcttc
     3361 caggcgcgca tcgacggcta cgccacggcc agcgcgctga gcttcttccg ccagttgctg
     3421 ctgcgcgaga agggcgccta ctggaccttc atcgtccaca ccggcgaacg caccctggtc
     3481 ggcgcctcgc ccgagcgaca catcagcgtg cgcgacggcc tggcggtgat gaacccgatc
     3541 agcggcacct accgctaccc gccggccggg ccgaacctgg ccgaggtcat ggaatttctc
     3601 gacaaccgca aggaagccga cgagctgtac atggtggtgg acgaggagct gaagatgatg
     3661 gcgcggatct gcgaggacgg cggccgggtg ctcggtccct acctcaagga gatggcgcac
     3721 ctggcgcaca ccgagtactt catcgagggc cagaccagcc gcgacgtgcg cgaggtgctg
     3781 cgcgagacgc tgttcgcccc gaccgtcacc ggcagcccgc tggaaagcgc ctgccgggtg
     3841 atccgccgct acgagccgca gggtcgcggc tactacagcg gggtggcggc gctgatcggc
     3901 ggtgatggcc agggcgggcg cacgctggac tcggcgatcc tgatccgcac cgccgagatc
     3961 gagggcgacg gccgcctgcg catcggcgtc ggctcgacca tcgtgcgtca ctccgatccc
     4021 ctgggcgagg cggcggaaag ccgggccaag gcttccgggc tgatcgccgc gctgaagagc
     4081 caggcgccgc agcgcctggg ctcgcacccg cacgtggtgg cggcgctggc cagccgcaac
     4141 gcgccgatcg ccgacttctg gctgcgcggc gccagcgagc gccagcagtt gcaggccgac
     4201 ctgtccggac gcgaggtgct gatcgtcgat gccgaggaca ccttcacctc gatgatcgcc
     4261 aagcagctca agtcgctggg cctgacggtg accgtgcgcg gcttccagga gccgtactcg
     4321 ttcgacggct acgacctggt gatcatgggc cccggcccag gcaaccccac cgagatcggc
     4381 cagccgaaga tcggccacct gcacctggcc atccgttcgc tgctcagcga gcgccggccg
     4441 ttcctcgccg tgtgcctgag ccaccaggtg ctgagcctgt gcctgggcct ggacctgcaa
     4501 cgccggcaag agcccaacca gggcgtgcag aaacagatcg acctgttcgg cgccgccgag
     4561 cgcgtcggct tctacaacac cttcgctgcg cgcgccttgc aggaccgcat cgagatcccc
     4621 gaggtcggcc cgatcgagat cagccgcgac cgcgagaccg gcgaggtcca tgccctgcgc
     4681 gggccgcgct tcgcctccat gcaattccac cccgaatcgg tgctgacccg cgagggtccg
     4741 cgcatcatcg ccgacctgct gcgtcacgcg ctggtcgagc ggcggccctg agccaggaga
     4801 gcccatgcac agatatgtcg tgatagacgc cttcgccagc gaaccgctgc aaggcaaccc
     4861 ggtggcggtg ttcttcgact gcgacgacct cagcggcgag cgcatgcagc gcatggcgcg
     4921 ggagatgaac ctgtcggaga gcaccttcgt gctccgcccg caacaggacg gcgacgcgcg
     4981 catccgcatc ttcaccccgg tcaacgaact gccgttcgcc ggccacccgc tgctgggcac
     5041 cgccatcgcc ctgggcgccg agaccgacaa ggacaggctg ttcctggaga ccaggatggg
     5101 caccgtgccc ttcgccctgg aacgccagga cggcaaggtg gtggcctgta gcatgcagca
     5161 gccgatcccc acctgggagc acttcagccg accggccgag ctgctcgccg ccctcggcct
     5221 gaagggctcg acctttccca tcgaggtcta ccgcaacggc ccgcgccacg tcttcgtcgg
     5281 cctggagagc gtcgccgcgc tgtccgcgct gcaccccgac caccgcgcgc tgtgcgactt
     5341 cccggacctg gcggtgaact gcttcgccgg cgccgggcgg cactggcgca gccgcatgtt
     5401 ctcgccggcc tacggcgtgg tcgaggacgc cgccaccggt tccgccgccg ggccgctggc
     5461 gatccacctg gcgcgccacc ggcagattcc ctacgggcag cagatcgaga tcctccaggg
     5521 cgtcgagatc ggccggccgt cgcgcatgta cgcccgcgcc gagggcgccg gcgagcgggt
     5581 ctcggcggtg gaagtgtcgg gcaacggcgc ggccttcgcc gaaggccgcg cctacctgtg
     5641 aacgggcaga acggaggaac gccgtgatgg gcgtcaacgc gaacatttcc gagtccctca
     5701 ccgggaccat cgaagcccct ttcccggagt tcgaggcgcc gccggcgaac cccatggagg
     5761 tgctgcgcaa ctggctggaa cgcgcccgcc gctacggcgt gcgcgagccg cgggcactgg
     5821 ccctggccac ggtcgacggg cagggccggc cgtcgacgcg gatcgtggtg atcgccgagc
     5881 tgggcgagcg cggggtggtg ttcgccaccc acgccgatag ccagaagggc cgcgaactgg
     5941 cgcagaaccc ctgggcctcg ggggtgctgt actggcgcga gagcagccag cagatcatcc
     6001 tcaacggccg cgccgaacgc ctgcccgacg agcgcgccga cgcccagtgg ctgagccgcc
     6061 cgtaccagac ccacccgatg tcgatcgcct cgcggcagag cgaaaccctc gccgacatcc
     6121 acgccctgcg cgccgaagcc cggcgcctgg cggaaaccga cggcccgctg ccgcgcccgc
     6181 cgggctactg cctgttcgaa ctgtgcctgg agtcggtgga gttctggggc aacggcaccg
     6241 agcggctgca cgagcgcctg cgctacgacc gcgacgaagg aggctggaag caccgctacc
     6301 tgcaaccgtg ataatactag tagcggccgc tgcaggcatg caagcttggc gtaatcatgg
     6361 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     6421 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     6481 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     6541 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     6601 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     6661 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     6721 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     6781 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     6841 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     6901 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     6961 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     7021 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     7081 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     7141 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     7201 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     7261 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     7321 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     7381 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     7441 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     7501 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     7561 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     7621 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     7681 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     7741 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     7801 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     7861 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     7921 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     7981 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     8041 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     8101 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     8161 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     8221 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     8281 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     8341 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     8401 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     8461 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     8521 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     8581 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     8641 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     8701 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
     8761 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
     8821 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     8881 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     8941 ttttcccagt cacgacgttg taaaacgacg gccagt
//
