LOCUS       sequence-310551-       10706 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pcDNA5 based transfection plasmid for the exogenous expression of 
            N-terminal BirA-Flag-tagged eIF4G1 including the microexon for 
            generation of N2A FlpIn cell lines for BioID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10706)
  AUTHORS   Gonatopoulos-Pournatzis T, Niibori R, Salter EW, Weatheritt RJ, 
            Tsang B, Farhangmehr S, Liang X, Braunschweig U, Roth J, Zhang S, 
            Henderson T, Sharma E, Quesnel-Vallieres M, Permanyer J, Maier S, 
            Georgiou J, Irimia M, Sonenberg N, Forman-Kay JD, Gingras AC, 
            Collingridge GL, Woodin MA, Cordes SP, Blencowe BJ
  TITLE     Autism-Misregulated eIF4G Microexons Control Synaptic Translation 
            and Higher Order Cognitive Functions.
  JOURNAL   Mol Cell. 2020 Mar 19;77(6):1176-1192.e16. doi: 
            10.1016/j.molcel.2020.01.006. Epub 2020 Jan 29.
  PUBMED    31999954
REFERENCE   2  (bases 1 to 10706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01.006"; journalName: "Mol Cell"; date: 
            "2020-03-19- 19"; volume: "77"; issue: "6"; pages: "1176-1192.e16"
COMMENT     Sequence Label: pcDNA5 rtTA BirA eIF4G +MIC
FEATURES             Location/Qualifiers
     source          1..10706
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    262..280
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    299..317
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    336..354
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    373..391
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    410..428
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    447..465
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    484..502
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    521..539
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     561..585
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(718..1068,1069..1071,1072..1680)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             1711..1734
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    1747..1771
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6592..6616)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(6658..6675)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6664..6888
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7021..7040)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    7172..7219
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7227..8249
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             7227..8249
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8379..8500
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8416..8435)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8470..8489
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8549..8565)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8549..8565)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8562..8584)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8573..8589
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8597..8603,8604..8621,8622..8627))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8642..8663
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8780..8797)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8951..9539)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9031..9050)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9710..10501,10502..10570))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10333..10352
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10571..10675)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtctcgagg
      241 gatccaagct ggccgcacgt ctccctatca gtgatagaga agtcgacacg tctcgagctc
      301 cctatcagtg atagagaagg tacgtctaga acgtctccct atcagtgata gagaagtcga
      361 cacgtctcga gctccctatc agtgatagag aaggtacgtc tagaacgtct ccctatcagt
      421 gatagagaag tcgacacgtc tcgagctccc tatcagtgat agagaaggta cgtctagaac
      481 gtctccctat cagtgataga gaagtcgaca cgtctcgagc tccctatcag tgatagagaa
      541 ggtaccccct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      601 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg cggaggctgg
      661 atcctctagc gtttaaactt aagcttggta ccgagctcgg atccagctag cgccaccatg
      721 aaggacaaca ccgtgcccct gaagctgatc gccctgctgg ccaacggcga gttccacagc
      781 ggcgagcagc tgggcgagac cctgggcatg agccgggccg ccatcaacaa gcacatccag
      841 accctgcggg actggggcgt ggacgtgttc accgtgcccg gcaagggcta cagcctgccc
      901 gagcccatcc agctgctgaa cgccaagcag atcctgggcc agctggacgg cggcagcgtg
      961 gccgtgctgc ccgtgatcga cagcaccaac cagtacctgc tggaccggat cggcgagctg
     1021 aagagcggcg acgcctgcat cgccgagtac cagcaggccg gccggggcgg ccggggccgg
     1081 aagtggttca gccccttcgg cgccaacctg tacctgagca tgttctggcg gctggagcag
     1141 ggccccgccg ccgccatcgg cctgagcctg gtgatcggca tcgtgatggc cgaggtgctg
     1201 cggaagctgg gcgccgacaa ggtgcgggtg aagtggccca acgacctgta cctgcaggac
     1261 cggaagctgg ccggcatcct ggtggagctg accggcaaga ccggcgacgc cgcccagatc
     1321 gtgatcggcg ccggcatcaa catggccatg cggcgggtgg aggagagcgt ggtgaaccag
     1381 ggctggatca ccctgcagga ggccggcatc aacctggacc ggaacaccct ggccgccatg
     1441 ctgatccggg agctgcgggc cgccctggag ctgttcgagc aggagggcct ggccccctac
     1501 ctgagccggt gggagaagct ggacaacttc atcaaccggc ccgtgaagct gatcatcggc
     1561 gacaaggaga tcttcggcat cagccggggc atcgacaagc agggcgccct gctgctggag
     1621 caggacggca tcatcaagcc ctggatgggc ggcgagatca gcctgcggag cgccgagaag
     1681 gggggatctg gccccggcgg cggcgcgcca gactacaaag acgatgacga taaactaggc
     1741 gcgcccacaa gtttgtacaa aaaagcaggc ttcaacaaag ctccacagtc cacaggcccc
     1801 ccacccgccc catcccccgg actcccacag ccagcgtttc ccccggggca gacagcgccg
     1861 gtggtgttca gtacgccaca agcgacacaa atgaacacgc cttctcagcc ccgccaggga
     1921 ggattcaggt ctctgcagca cttctaccct agccgggccc agcccccgag cagtgcagcc
     1981 tcccgagtgc agagtgcagc ccctgcccgc cctggcccag ctgcccatgt ctaccctgct
     2041 ggatcccaag taatgatgat cccttcccag atctcctacc cagcctccca gggggcctac
     2101 tacatccctg gacaggggcg ttccacatac gttgtcccga cacagcagta ccctgtgcag
     2161 ccaggagccc caggcttcta tccaggtgca agccctacag aatttgggac ctacgctggc
     2221 gcctactatc cagcccaagg ggtgcagcag tttcccactg gcgtggcccc cgccccagtt
     2281 ttgatgaacc agccacccca gattgctccc aagagggagc gtaagacgat ccgaattcga
     2341 gatccaaacc aaggaggaaa ggatatcaca gaggagatca tgtctggggc ccgcactgcc
     2401 tccacaccca cccctcccca gacgggaggc ggtctggagc ctcaagctaa tggggagacg
     2461 ccccaggttg ctgtcattgt ccggccagat gaccggtcac agggagcaat cattgctgac
     2521 cggccagggc tgcctggccc agagcatagc ccttcagaat cccagccttc gtcgccttct
     2581 ccgaccccat caccatcccc agtcttggaa ccggggtctg agcctaatct cgcagtcctc
     2641 tctattcctg gggacactat gacaactata caaatgtctg tagaagaatc aacccccatc
     2701 tcccgtgaaa ctggggagcc atatcgcctc tctccagaac ccactcctct cgccgaaccc
     2761 atactggaag tagaagtgac acttagcaaa ccggttccag aatctgagtt ttcttccagt
     2821 cctctccagg ctcccacccc tttggcatct cacacagtgg aaattcatga gcctaatggc
     2881 atggtcccat ctgaagatct ggaaccagag gtggagtcaa gcccagagct tgctcctccc
     2941 ccagcttgcc cctccgaatc ccctgtgccc attgctccaa ctgcccaacc tgaggaactg
     3001 ctcaacggag ccccctcgcc accagctgtg gacttaagcc cagtcagtga gccagaggag
     3061 caggccaagg aggtgacagc atcagtggcg ccccccacca tcccctctgc tactccagct
     3121 acggctcctt cagctacttc cccagctcag gaggaggaaa tggaagaaga agaagaagag
     3181 gaagaaggag aagcaggaga agcaggagaa gctgagagtg agaaaggagg agaggaactg
     3241 ctccccccag agagtacccc tattccagcc aacttgtctc agaatttgga ggcagcagca
     3301 gccactcaag tggcagtatc tgtgccaaag aggagacgga aaattaagga gctaaataag
     3361 aaggaggctg ttggagacct tctggatgcc ttcaaggagg cgaacccggc agtaccagag
     3421 gtggaaaatc agcctcctgc aggcagcaat ccaggcccag agtctgaggg cagtggtgtg
     3481 cccccacgtc ctgaggaagc agatgagacc tgggactcaa aggaagacaa aattcacaat
     3541 gctgagaaca tccagcccgg ggaacagaag tatgaatata agtcagatca gtggaagcct
     3601 ctaaacctag aggagaaaaa acgttacgac cgtgagttcc tgcttggttt tcagttcatc
     3661 tttgccagta tgcagaagcc agagggattg ccacatatca gtgacgtggt gctggacaag
     3721 gccaataaaa caccactgcg gccactggat cccactagac tacaaggcat aaattgtggc
     3781 ccagacttca ctccatcctt tgccaacctt ggccggacaa cccttagcac ccgtgggccc
     3841 ccaaggggtg ggccaggtgg ggagctgccc cgtgggccgg ctggcctggg accccggcgc
     3901 tctcagcagg gaccccgaaa agaaccacgc aagatcattg ccacagtgtt aatgaccgaa
     3961 gatataaaac tgaacaaagc agagaaagcc tggaaaccca gcagcaagcg gacggcggct
     4021 gataaggatc gaggggaaga agatgctgat ggcagcaaaa cccaggacct attccgcagg
     4081 gtgcgctcca tcctgaataa actgacaccc cagatgttcc agcagctgat gaagcaagtg
     4141 acgcagctgg ccatcgacac cgaggaacgc ctcaaagggg tcattgacct catttttgag
     4201 aaggccattt cagagcccaa cttctctgtg gcctatgcca acatgtgccg ctgcctcatg
     4261 gcgctgaaag tgcccactac ggaaaagcca acagtgactg tgaacttccg aaagctgttg
     4321 ttgaatcgat gtcagaagga gtttgagaaa gacaaagatg atgatgaggt ttttgagaag
     4381 aagcaaaaag agatggatga agctgctacg gcagaggaac gaggacgcct gaaggaagag
     4441 ctggaagagg ctcgggacat agcccggcgg cgctctttag ggaatatcaa gtttattgga
     4501 gagttgttca aactgaagat gttaacagag gcaataatgc atgactgtgt ggtcaaactg
     4561 cttaagaacc atgatgaaga gtcccttgag tgcctttgtc gtctgctcac caccattggc
     4621 aaagacctgg actttgaaaa agccaagccc cgaatggatc agtatttcaa ccagatggaa
     4681 aaaatcatta aagaaaagaa gacgtcatcc cgcatccgct ttatgctgca ggacgtgctg
     4741 gatctgcgag ggagcaattg ggtgccacgc cgaggggatc agggtcccaa gaccattgac
     4801 cagatccata aggaggctga gatggaagaa catcgagagc acatcaaagt gcagcagctc
     4861 atggccaagg gcagtgacaa gcgtcggggc ggtcctccag gccctcccat cagccgtgga
     4921 cttccccttg tggatgatgg tggctggaac acagttccca tcagcaaagg tagccgcccc
     4981 attgacacct cacgactcac caagatcacc aagcctggct ccatcgattc taacaaccag
     5041 ctctttgcac ctggagggcg actgagctgg ggcaagggca gcagcggagg ctcaggagcc
     5101 aagccctcag acgcagcatc agaagctgct cgcccagcta ctagtacttt gaatcgcttc
     5161 tcagcccttc aacaagcggt acccacagaa agcacagata atagacgtgt ggtgcagagg
     5221 agtagcttga gccgagaacg aggcgagaaa gctggagacc gaggagaccg cctagagcgg
     5281 agtgaacggg gaggggaccg tggggaccgg cttgatcgtg cgcggacacc tgctaccaag
     5341 cggagcttca gcaaggaagt ggaggagcgg agtagagaac ggccctccca gcctgagggg
     5401 ctgcgcaagg cagctagcct cacggaggat cgggaccgtg ggcgggatgc cgtgaagcga
     5461 gaagctgccc tacccccagt gagccccctg aaggcggctc tctctgagga ggagttagag
     5521 aagaaatcca aggctatcat tgaggaatat ctccatctca atgacatgaa agaggcagtc
     5581 cagtgcgtgc aggagctggc ctcaccctcc ttgctcttca tctttgtacg gcatggtgtc
     5641 gagtctacgc tggagcgcag tgccattgct cgtgagcata tggggcagct gctgcaccag
     5701 ctgctctgtg ctgggcatct gtctactgct cagtactacc aagggttgta tgaaatcttg
     5761 gaattggctg aggacatgga aattgacatc ccccacgtgt ggctctacct agcggaactg
     5821 gtaacaccca ttctgcagga aggtggggtg cccatggggg agctgttcag ggagattaca
     5881 aagcctctga gaccgttggg caaagctgct tccctgttgc tggagatcct gggcctcctg
     5941 tgcaaaagca tgggtcctaa aaaggtgggg acgctgtggc gagaagccgg gcttagctgg
     6001 aaggaatttc tacctgaagg ccaggacatt ggtgcattcg tcgctgaaca gaaggtggag
     6061 tataccctgg gagaggagtc ggaagcccct ggccagaggg cactcccctc cgaggagctg
     6121 aacaggcagc tggagaagct gctgaaggag ggcagcagta accagcgggt gttcgactgg
     6181 atagaggcca acctgagtga gcagcagata gtatccaaca cgttagttcg agccctcatg
     6241 acggctgtct gctattctgc aattattttt gagactcccc tccgagtgga cgttgcagtg
     6301 ctgaaagcgc gagcgaagct gctgcagaaa tacctgtgtg acgagcagaa ggagctacag
     6361 gcgctctacg ccctccaggc ccttgtagtg accttagaac agcctcccaa cctgctgcgg
     6421 atgttctttg acgcactgta tgacgaggac gtggtgaagg aggatgcctt ctacagttgg
     6481 gagagtagca aggaccccgc tgagcagcag ggcaagggtg tggcccttaa atctgtcaca
     6541 gccttcttca agtggctccg tgaagcagag gaggagtctg accacaactg atgacagctt
     6601 tcttgtacaa agtggtgtga ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct
     6661 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     6721 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     6781 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     6841 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     6901 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     6961 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     7021 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     7081 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     7141 aacttgatta gggtgatggt tcacgtacct agaagttcct attccgaagt tcctattctc
     7201 tagaaagtat aggaacttcc ttggccaaaa agcctgaact caccgcgacg tctgtcgaga
     7261 agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag
     7321 aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct
     7381 gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc
     7441 cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc
     7501 gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc
     7561 agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt
     7621 tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg
     7681 cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt
     7741 ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc
     7801 acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag
     7861 cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct
     7921 tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc
     7981 atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc
     8041 aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat
     8101 gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa
     8161 gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc
     8221 ccagcactcg tccgagggca aaggaatagc acgtactacg agatttcgat tccaccgccg
     8281 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     8341 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     8401 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     8461 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     8521 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     8581 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     8641 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     8701 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     8761 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     8821 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     8881 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     8941 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     9001 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     9061 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     9121 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     9181 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     9241 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     9301 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     9361 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     9421 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     9481 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     9541 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     9601 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     9661 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     9721 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     9781 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     9841 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     9901 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     9961 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
    10021 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
    10081 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
    10141 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
    10201 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
    10261 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    10321 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    10381 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    10441 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    10501 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    10561 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    10621 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    10681 ttccccgaaa agtgccacct gacgtc
//
