LOCUS       sequence-310555-        8509 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid for expression of shorter LifeAct variant (up 
            till 14 amino acids) fused with carboxy terminus eGFP in mammalian 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8509)
  AUTHORS   Kumari A, Kesarwani S, Javoor MG, Vinothkumar KR, Sirajuddin M
  TITLE     Structural insights into actin filament recognition by commonly used
            cellular actin markers.
  JOURNAL   EMBO J. 2020 Jun 22:e104006. doi: 10.15252/embj.2019104006.
  PUBMED    32567727
REFERENCE   2  (bases 1 to 8509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2020 Jun 22:e104006. doi: 10.15252/embj.2019104006."
COMMENT     Sequence Label: LifeAct-14 (LA-14)
FEATURES             Location/Qualifiers
     source          1..8509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      281..290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      344..353
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(350..352,353..355,356..1069)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(350..352,353..355,356..1066)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(395..416)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(656..675)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1003..1024
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1097..1117
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    complement(1229..1253)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1284..1872
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1337..1357)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1755..1766)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        1886..2385
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1921..1944)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2318..2337
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2419..2817
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             2914..3147
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3219..3340
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3256..3275)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3310..3329
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3380..3515
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3535..3554)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3536..3554)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3564..3581)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3564..3580)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3573..3595)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3722..4177
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3809..3828)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4019..4040
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4203..4307
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4308..4376,4377..5168)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4526..4545)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5339..5927
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5828..5847
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6081..6098
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6215..6236
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6251..6256,6257..6274,6275..6281)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6289..6305
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6294..6316
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6313..6329
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6313..6329
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6348..6368
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6350..6368
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6396..6622
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6623..6803
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6850..6975
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7468..7701
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7886..7930
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8197..8313
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    8370..8394
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggttt atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      241 ccacgctgtt ttgacctcca tagaagattc tagagctagc gccaccatgg gcgtggccga
      301 cttgatcaag aagttcgagt ccatctccgg ggatccaccg gtcgccacca tggtgagcaa
      361 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
      421 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
      481 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
      541 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
      601 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
      661 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
      721 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
      781 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
      841 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
      901 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
      961 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     1021 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaaa gcggcctcga
     1081 gcatgcatct agaggacaac tttgtataca aaagttggga gacccaagct tggtaccgag
     1141 ctcggatcca ctagtaacgg ccgccagtgt gctggaattc tgcagatatc catcacactg
     1201 gcggccgctc gagcatgcat ctagaggtac ccagctttct tgtacaaagt ggttgatatc
     1261 cagcacagtg gcggccgctc gacaatcaac ctctggatta caaaatttgt gaaagattga
     1321 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     1381 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     1441 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     1501 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     1561 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     1621 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc
     1681 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     1741 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     1801 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     1861 ccgcctcccc gcctggaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga
     1921 gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc
     1981 acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg
     2041 ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc
     2101 aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct
     2161 gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct
     2221 ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg
     2281 ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc
     2341 acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagcccaagc
     2401 ttaccacact cctgcaggat ggccaagcct ttgtctcaag aagaatccac cctcattgaa
     2461 agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt cgccagcgca
     2521 gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt tactggggga
     2581 ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg caacctgact
     2641 tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg acggtgccga
     2701 caggtgcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag tgatggacag
     2761 ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga gggctaagca
     2821 cgcgtatgca tctcgagggc ccggtacctt taagaccaat gacttacaag gcagctgtag
     2881 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctagctcac tcccaacgaa
     2941 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     3001 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     3061 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     3121 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     3181 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     3241 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     3301 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     3361 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     3421 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     3481 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     3541 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     3601 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     3661 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     3721 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     3781 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     3841 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     3901 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     3961 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     4021 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     4081 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     4141 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     4201 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     4261 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     4321 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     4381 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     4441 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     4501 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     4561 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     4621 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     4681 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     4741 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     4801 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     4861 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     4921 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     4981 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     5041 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     5101 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     5161 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     5221 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     5281 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     5341 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     5401 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     5461 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     5521 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     5581 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     5641 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     5701 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     5761 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     5821 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     5881 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     5941 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     6001 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     6061 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     6121 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     6181 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     6241 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     6301 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     6361 actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta
     6421 gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg
     6481 tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac
     6541 gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa
     6601 gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct
     6661 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     6721 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     6781 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     6841 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     6901 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     6961 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     7021 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     7081 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     7141 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     7201 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     7261 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
     7321 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     7381 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     7441 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     7501 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     7561 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     7621 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     7681 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     7741 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     7801 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     7861 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     7921 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     7981 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     8041 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     8101 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
     8161 tcgattagtg aacggatctc gacggtatcg gttaactttt aaaagaaaag gggggattgg
     8221 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     8281 attacaaaaa caaattacaa aattcaaaat tttatcgtcc agtgtggtgg aattgagtat
     8341 tccagtgtgg tggaattctg cagatatcaa caagtttgta caaaaaagca ggctggagac
     8401 ccaagcttgg taccgagctc ggatccacta gtattatgcc cagtacatga ccttatggga
     8461 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatg
//
