LOCUS       sequence-310556-        4383 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA Kit # 1000000119 extension.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4383)
  TITLE     Lewin Lab Plasmids
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRDAX-VALENTIA-016   NLSNoLS-Sirius//BsaI
FEATURES             Location/Qualifiers
     source          1..4383
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        158..537
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        539..814
                     /label=chicken beta-actin promoter
     CDS             843..890
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     polyA_signal    1767..1888
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1804..1823)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1858..1877
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     terminator      2689..2718
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      2740..3327
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3228..3247
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3339..3366)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(join(3390..4181,4182..4250))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4013..4032
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4251..4353)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(join(4354..4383,1..2))
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 ctttctgcta tggaggtcag gtatgattta aaggtctcat aggtcttgta ttcagtctcg
       61 cactttagac atggtcttct gctgcccgtg gtcctctatt cgtcggatgt tctgtcgatt
      121 cttcctgtct atctggactg gatctgatat catcgtcgac attgattatt gactagttat
      181 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      241 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      301 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      361 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      421 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      481 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
      541 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
      601 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg ggggggggcg
      661 cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc
      721 ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg
      781 gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgttgg gacgccacca
      841 tgaaacggcc tgctgccacc aagaaagcag gacaggccaa gaagaagaaa cgcaggagaa
      901 ggcgacgtag aggcggtagc gggggcagcg tgagcaaagg agaggagctc tttacaggag
      961 tagtcccgat attggtggaa cttgatggcg acgtcaatgg acaccgtttc tccgtgagtg
     1021 gagaaggcga aggcgatgca acctatggta aactcaccct gaagcttatc tgcactacgg
     1081 ggaaactgcc agtaccttgg ccaaccctgg ttacaaccct gcagtttggg gttctgtgtt
     1141 ttgcccgata tcccgatcac atgaagcagc atgacttctt caagtcagcc atgccagaag
     1201 ggtatgtcca agagaggacc atcttcttta aagacgatgg caactataag acgagagcag
     1261 aagtgaagtt tgagggagac actctggtga atcggattga actcaaaggc atcgacttca
     1321 aggaagatgg caatatcctg ggtcacaaac tggagtacaa tgggatctct agcaacgtgt
     1381 acatcacagc cgataagcag aagaacggga taaaggcgca tttcaagatt cgccataaca
     1441 tcgaggacgg tggcgttcaa ctcgctgatc actaccagca gaacactccc attggcgatg
     1501 gacctgtgct tctccctgac aaccactact tgtctgtcca gagcaagctg tccaaagacc
     1561 ccaatgagaa gagggaccat atggtcttgc tggagagtgt gacagctgct gggattactc
     1621 tgggtatgga cgagctgtac aaatcaggct ctgattaagg cgccaggtac atctccaata
     1681 cggcgtcttg catagagaac acgattagca caccggttac ccacgtgatg cccgaacgga
     1741 cccatgtcat gccagagcgc actggtaact tgtttattgc agcttataat ggttacaaat
     1801 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     1861 gtttgtccaa actcatcaat gtatcttatc atgtctgtcg tcgaattcga gttggcgcgc
     1921 ctgtcattct aaatctctct ttcagcctaa agctttttcc ccgtatcccc ccaggtgtct
     1981 gcaggctcaa agagcagcga gaagcgttca gaggaaagcg atcccgtgcc accttccccg
     2041 tgcccgggct gtccccgcac gctgccggct cggggatgcg gggggagcgc cggaccggag
     2101 cggagccccg ggcggctcgc tgctgccccc tagcggggga gggacgtaat tacatccctg
     2161 ggggctttgg gagggggctg tccccgtgag ctcccaggcg cgcctgtcat tctaaatctc
     2221 tctttcagcc taaagctttt tccccgtatc cccccaggtg tctgcaggct caaagagcag
     2281 cgagaagcgt tcagaggaaa gcgatcccgt gccaccttcc ccgtgcccgg gctgtccccg
     2341 cacgctgccg gctcggggat gcggggggag cgccggaccg gagcggagcc ccgggcggct
     2401 cgctgctgcc ccctagcggg ggagggacgt aattacatcc ctgggggctt tggggggggg
     2461 ctgtccccgt gagctcccag gcgcgccaac tcggtacctc acccgattct catcatggag
     2521 acacatcagg acatctccaa taccgccatg gaaacgcacc aagatgcgac tgctcttctg
     2581 ccagtgacgg cattcgtctg caagattaac ggcatacaga aagaaacgat gagacccatt
     2641 caggacgagc ctcagactcc agcgtaactg gactgcaatc aactcactgg ctcaccttca
     2701 cgggtgggcc tttcttcggt agaaaatcaa aggatcttct tgagatcctt tttttctgcg
     2761 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     2821 tcaagagcta ccaactcttt ttccgaggta actggcttca gcagagcgca gataccaaat
     2881 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     2941 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     3001 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     3061 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     3121 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     3181 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     3241 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcatcgatt tttgtgatgc
     3301 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cagaaaggcc cacccgaagg
     3361 tgagccaggt gattacattt gggccctcat taccaatgct taatcagtga ggcacctatc
     3421 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     3481 acgatgcggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agaaccacgc
     3541 tcaccggctc cagatttatc agcaataaac cagccagccg ggagggccga gcgcagaagt
     3601 gatcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     3661 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     3721 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     3781 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgcc
     3841 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     3901 actgtcatgc catccgtgag atgcttttct gtgactggtg agtactcaac caagtcattc
     3961 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     4021 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgtaaa
     4081 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     4141 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     4201 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat tttagcttcc
     4261 ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta
     4321 tggtgaaagt tggaacctct tacgtgccga tcaagtcaaa agcctccggt cggaggcttt
     4381 tga
//
