LOCUS       sequence-310561-       11795 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for expression of TagBFP_A1aY1 in mammalian cells 
            for detection of tyrosinated microtubules..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11795)
  AUTHORS   Kesarwani S, Lama P, Chandra A, Reddy PP, Jijumon AS, Bodakuntla S, 
            Rao BM, Janke C, Das R, Sirajuddin M
  TITLE     Genetically encoded live-cell sensor for tyrosinated microtubules.
  JOURNAL   J Cell Biol. 2020 Oct 5;219(10). pii: 152071. doi: 
            10.1083/jcb.201912107.
  PUBMED    32886100
REFERENCE   2  (bases 1 to 11795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-10-5"; volume: "219(10). pii: 152071. doi"; 
            pages: " 10.1083/jcb.201912107"
COMMENT     Sequence Label: TagBFP_A1aY1
FEATURES             Location/Qualifiers
     source          1..11795
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..657
                     /label=chicken beta-actin promoter
     intron          658..1674
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1598..1620
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1682..1701
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1745..2443
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     LTR             2943..3123
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4651..4670)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4884..4903)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5003..5025
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5063..5081)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5149..5253
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5254..5322,5323..6114)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5472..5491)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6285..6873
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6774..6793
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7027..7044
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7114..7134)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7119..7448
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7299..7434
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8635..8700
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8830..8850
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9275..9409
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9312..9331)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9366..9385
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9577..10210
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10257..10382
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10875..11108
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11293..11337
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11630..11747
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gggcgcgccr gggggggggg gggggggsgr ggggsggggs
      541 ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct
      601 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga
      661 gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc
      721 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
      781 ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc
      841 cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg
      901 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
      961 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
     1081 gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc
     1321 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
     1381 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
     1441 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
     1561 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1621 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1681 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattatt ccgctagcgc
     1741 caccatgagc gagctgatta aggagaacat gcacatgaag ctgtacatgg agggcaccgt
     1801 ggacaaccat cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca
     1861 gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc
     1921 tactagcttc ctctacggca gcaagacctt catcaaccac acccagggca tccccgactt
     1981 cttcaagcag tccttccctg agggcttcac atgggagaga gtcaccacat acgaagacgg
     2041 gggcgtgctg accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt
     2101 caagatcaga ggggtgaact tcacatccaa cggccctgtg atgcagaaga aaacactcgg
     2161 ctgggaggcc ttcaccgaga cgctgtaccc cgctgacggc ggcctggaag gcagaaacga
     2221 catggccctg aagctcgtgg gcgggagcca tctgatcgca aacatcaaga ccacatatag
     2281 atccaagaaa cccgctaaga acctcaagat gcctggcgtc tactatgtgg actacagact
     2341 ggaaagaatc aaggaggcca acaacgagac ctacgtcgag cagcacgagg tggcagtggc
     2401 cagatactgc gacctcccta gcaaactggg gcacaagctt aattccggac tcagatctcg
     2461 agtggcaaca gtcaagttca aatacaaggg ggaggaaaag caggtggaca ttagtaagat
     2521 tctgagcgtc ggaagatacg ggaagctgat ccacttcctg tacgacctgg gaggaggcaa
     2581 ggcaggaatg ggcatggtga gcgagaagga tgcccccaag gagctgctcc agatgctgga
     2641 gaagcagaag aagtaaggat ccactagtgt cgacctcgag acctagaaaa acatggagca
     2701 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag
     2761 gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag
     2821 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     2881 tcccaacgaa gacaaaatcg tcgagagatg ctgcatataa gcagctgctt tttgcttgta
     2941 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     3001 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     3061 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     3121 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     3181 cagagagtga gaggccttga cattataata gatttagcag gaattgaact aggagtggag
     3241 cacacaggca aagctgcaga agtacttgga agaagccacc agagatactc acgattctgc
     3301 acatacctgg ctaatcccag atcctaagga ttacattaag tttactaaca tttatataat
     3361 gatttatagt ttaaagtata aacttatcta atttactatt ctgacagata ttaattaatc
     3421 ctcaaatatc ataagagatg attactatta tccccattta acacaagagg aaactgagag
     3481 ggaaagatgt tgaagtaatt ttcccacaat tacagcatcc gttagttacg actctatgat
     3541 cttctgacac aaattccatt tactcctcac cctatgactc agtcgaatat atcaaagtta
     3601 tggacattat gctaagtaac aaattaccct tttatatagt aaatactgag tagattgaga
     3661 gaagaaattg tttgcaaacc tgaatagctt caagaagaag agaagtgagg ataagaataa
     3721 cagttgtcat ttaacaagtt ttaacaagta acttggttag aaagggattc aaatgcataa
     3781 agcaagggat aaatttttct ggcaacaaga ctatacaata taaccttaaa tatgacttca
     3841 aataattgtt ggaacttgat aaaactaatt aaatattatt gaagattatc aatattataa
     3901 atgtaattta cttttaaaaa gggaacatag aaatgtgtat cattagagta gaaaacaatc
     3961 cttattatca caatttgtca aaacaagttt gttattaaca caagtagaat actgcattca
     4021 attaagttga ctgcagattt tgtgttttgt taaaattaga aagagataac aacaatttga
     4081 attattgaaa gtaacatgta aatagttcta catacgttct tttgacatct tgttcaatca
     4141 ttgatcgaag ttctttatct tggaagaatt tgttccaaag actctgaaat aaggaaaaca
     4201 atctattata tagtctcaca cctttgtttt acttttagtg atttcaattt aataatgtaa
     4261 atggttaaaa tttattcttc tctgagatca tttcacattg cagatagaaa acctgagact
     4321 ggggtaattt ttattaaaat ctaatttaat ctcagaaaca catctttatt ctaacatcaa
     4381 tttttccagt ttgatattat catataaagt cagccttcct catctgcagg ttccacaaca
     4441 aaaatccaac caactgtgga tcaaaaatat tgggaaaaaa ttaaaaatag caatacaaca
     4501 ataaaaaaat acaaatcaga aaaacagcac agtataacaa ctttatttag catttacaat
     4561 ctattaggta ttataagtaa tctagccaga tcctctacgc cggacgcatc gtggccggca
     4621 tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag
     4681 atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc
     4741 ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg
     4801 tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag
     4861 agcgtcgaat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     4921 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     4981 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     5041 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     5101 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     5161 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     5221 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     5281 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     5341 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     5401 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     5461 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     5521 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     5581 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     5641 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     5701 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     5761 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     5821 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     5881 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     5941 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     6001 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     6061 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     6121 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     6181 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     6241 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6301 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6361 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6421 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6481 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6541 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6601 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6661 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     6721 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     6781 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     6841 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     6901 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     6961 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     7021 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     7081 cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt gtgtcagtta gggtgtggaa
     7141 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     7201 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     7261 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     7321 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     7381 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     7441 tttgcaaaaa gcttggacac aagacaggct tgcgagatat gtttgagaat accactttat
     7501 cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat actggttttt
     7561 agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac tttttaagcc
     7621 gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac ctgggacatg
     7681 ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca atggaaaggc
     7741 attattgccg taagccgtgg cggtctgtac cgggtgcgtt actggcgcgt gaactgggta
     7801 ttcgtcatgt cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta
     7861 aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtgg
     7921 ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca
     7981 tcttcgcaaa accggctggt cgtccgctgg ttgatgacta tgttgttgat atcccgcaag
     8041 atacctggat tgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc
     8101 gctaatcttt tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcgggtta
     8161 caatagtttc cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa
     8221 accctgttca aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttaat
     8281 cacggcgcac aaccgcctgt gcagtcggcc cttgatggta aaaccatccc tcactggtat
     8341 cgcatgatta accgtctgat gtggatctgg cgcggcattg acccacgcga aatcctcgac
     8401 gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg acgatgattt atacgatacg
     8461 gtgattggct accgtggcgg caactggatt tatgagtggg ccccggatct ttgtgaagga
     8521 accttacttc tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa
     8581 ggywrwtrtr wraywtwwww gkryawamtr ystacagaga tttaaagctc taaggtaaat
     8641 ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag
     8701 attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac
     8761 ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat
     8821 tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg
     8881 ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac
     8941 accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc
     9001 tttataagta ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg
     9061 catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt
     9121 tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag
     9181 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     9241 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     9301 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     9361 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac
     9421 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta
     9481 cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa
     9541 ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa
     9601 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc
     9661 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc
     9721 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt
     9781 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt
     9841 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga
     9901 actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg
     9961 gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt
    10021 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
    10081 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
    10141 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
    10201 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
    10261 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
    10321 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
    10381 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
    10441 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
    10501 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
    10561 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
    10621 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
    10681 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct
    10741 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
    10801 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
    10861 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
    10921 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
    10981 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
    11041 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
    11101 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
    11161 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
    11221 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
    11281 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
    11341 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
    11401 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
    11461 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
    11521 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
    11581 ggatctcgac ggtatcgccg aattcacaaa tggcagtatt catccacaat tttaaaagaa
    11641 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
    11701 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
    11761 gggacagcag agatccactt tggctgatac gcgtt
//
