LOCUS       sequence-310564-        6069 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  V5-tagged sequence of mUty cloned into entry vector for 
            recombination.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Deschepper CF
  TITLE     Regulatory effects of the Uty/Ddx3y locus on neighboring chromosome 
            Y genes and autosomal mRNA transcripts in adult mouse 
            non-reproductive cells.
  JOURNAL   Sci Rep. 2020 Sep 10;10(1):14900. doi: 10.1038/s41598-020-71447-3.
  PUBMED    32913328
REFERENCE   2  (bases 1 to 6069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2020-09-10"; volume: "10(1):14900. doi"; pages: " 
            10.1038/s41598-020-71447-3"
COMMENT     Sequence Label: pENTR1A_no_ccDB_mUty
FEATURES             Location/Qualifiers
     source          1..6069
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      754..840
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      932..959
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     967..990
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    1009..1108
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4777..4818
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    complement(4949..5048)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(5160..5179)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             5171..5980
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5238..5257)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgctagc atggatctcg gggacgtcta
      721 actactaagc gagagtaggg aactgccagg catcaaataa aacgaaaggc tcagtcggaa
      781 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat
      841 ccgccgggag cggatttgaa cgttgtgaag caacggcccg gagggtggcg ggcaggacgc
      901 ccgccataaa ctgccaggca tcaaactaag cagaaggcca tcctgacgga tggccttttt
      961 gcgtttctac aaactcttcc tgttagttag ttacttaagc tcgggcccca aataatgatt
     1021 ttattttgac tgatagtgac ctgttcgttg caacaaattg ataagcaatg cttttttata
     1081 atgccaactt tgtacaaaaa agcaggcttt aaaggaacca attcagtcga cgccgccacc
     1141 atgaaatctt acggattatc tctcactact gctgccctag gtaatgagga aaagaaaatg
     1201 gcggcggaaa aggctagagg cgagggcgaa gaggggtcct tcagtctcac agtcgaagag
     1261 aagaaggcgc tttgtggatt agatagcttc ttcgggttct tgacccgatg caaagatggc
     1321 gccaagatga agacgctgtt gaacaaggca attcacttct atgagtcttt aatagtaaaa
     1381 gctgaaggaa aagtggaatc agatttcttt tgtcaattag gtcacttcaa tctcttactg
     1441 gaagattatt caaaagcatt gtcatcttac cagagatact acagtttaca gactgactac
     1501 tggaagaatg ctgccttttt atatggtctt ggtttggtct atttctacta caatgcattt
     1561 cagtgggcaa ttagagcttt tcaggaagtc ctttatgttg accctaattt ttgcagagcc
     1621 aaagaaatcc atttacgact tggttttatg ttcaaaatga atacagatta tgaatctagt
     1681 ttaaagcatt ttcagctggc cctgatcgac tgtaatgtct gtactttgtc cagtgtcgaa
     1741 attcagtttc atattgctca cttatatgaa acccagagga aataccattc tgcaaaggca
     1801 gcctatgaac aacttttaca gattgaaagc ttgccatcac aagtcaaagc aactgtattg
     1861 caacagttag gttggatgca ccataatatg gatctaattg gagacaatac caccaaagag
     1921 agatatgcta ttcagtatct ccaaaagtca ttggaggaag atcctaattc tggtcaatct
     1981 tggtatttcc ttggaaggtg ttactcatgt attgggaagg ttcaggatgc ctttgtatct
     2041 tacaggcaat ccattgataa atcagaagca agtgcagaca catggtgttc aataggtgtg
     2101 ctgtatcaac agcaaaatca acctatggat gccttgcaag cctatatatg tgctgtacag
     2161 ttggatcatg gacatgcagc agcctggatg gacttaggta ttctttatga atcttgcaac
     2221 caaccccagg atgccattaa atgctactta aatgcagctc ggagcaaatc ttgtaataac
     2281 acctctgcac ttacatcaag aattaagttt ttacaggctc agttgtgtaa ccttccacaa
     2341 agtagtctac agaataaaac taaattactt cctagtattg aggaggcatg gagcctacca
     2401 atacccgcag agcttacctc caggcagggt gccatgaaca cagcacagca gagtgtttct
     2461 gatacctgga atagtgtcca gacagcttca catcattcag tacagcagaa agtttataca
     2521 cagtgcttca cagcacagaa attacagagt tttggtaaag atcaacaacc tccatttcaa
     2581 actgggtcca cacgatatct ccaagcagct agcactaatg atcagaatca gaatggaaat
     2641 catactctgc ctcagaattc aaaaggggat gctcaaaatc acttcttacg tattcctacc
     2701 tcagaagaac aaaagatcat taactttacc aaagagagca aggattcaag aagtaaatcc
     2761 ttgacttcta aaacaagtag gaaagataga gacacatcta atatctgtgt taatgcaaag
     2821 aagcattcta atcatattta tcagatctca tcagttccga tttccagcct taataataaa
     2881 gaatctgtgt caccagattt aataattgta gacaatcctc agctttctgt cttagttgga
     2941 gaaactatcg acaatgtgga ccatgacatt ggaacttgtg acaaagtcaa taatgttcat
     3001 ctagccattc acaaaaagcc tgataatctt tctgcttctt caccctcttc agccatttcc
     3061 acagaaacac tttctctaaa gctcactgaa cagacacaca ttgttaccag ctttatcagt
     3121 cctcacagtg gattacacac aattaatgga gaagggcatg agaacttaga gagttctgcc
     3181 agtgttaatg ttggcctcag acctagatct caaatcatac catcaatgtc tgtgtccata
     3241 tactccagtt caacagaagt tctgaaagcg tgcaggagtc taggtaaaaa tggtttgtct
     3301 aatggtcata ttctcttgga tatatgccca cctccaagac caccaacttc gccatatcct
     3361 cctttaccaa aggagaagtt gaatccaccc actcctagta tttatttgga aaataagcga
     3421 gatgcctttt ttcctccatt acatcaattt tgcatcaatc caaaaaaccc tgttacagta
     3481 attcgcggtc ttgctggagc tcttaagtta gatcttggac ttttctctac caaaactttg
     3541 gtagaggcta acaatgaaca tatcgtagaa gtgaggaccc aattgttaca gccagcagat
     3601 gaaaactggg atccctctgg aacaaagaaa atctggcgat atgaaaataa aagttctcat
     3661 actacaattg ctaaatatgc acagtaccag gcctgctcct tccaggaatc attgagagaa
     3721 gaaaatgaaa gaagaacaca agttaaagac tattcagata atgaatcaac atgttcagat
     3781 aattctggaa ggagacagaa agcacccttt aaaaccataa aatgtgggat taacattgat
     3841 ctgtctgata acaaaaagtg gaagttgcag ttacatgagc tgacaaagct tcctgctttt
     3901 gtacgtgttg tatcagcagg gaatcttctc agtcatgttg gctatactat attggggatg
     3961 aattcagttc aactatgtat gaaagttcca gggagtagaa taccaggtca ccaagaaaat
     4021 aacaatttct gttctgttaa cataaatatt ggtccaggtg attgtgaatg gtttgttgta
     4081 cctgaagatt attggggtgt tctgaatgac ttctgtgaaa aaaataattt gaatttcctg
     4141 atgagttctt ggtggccgaa ccttgaagat ctttatgaag caaatgttcc tgtttatagg
     4201 tttattcaac gacctggtga tttggtttgg ataaatgcag gcactgttca ctgggttcag
     4261 gctattgggt ggtgtaacaa catcacctgg aatgttggtc cactcacagc cttccaatat
     4321 aaattggcag ttgaacgata tgaatggaat aaattgcaaa gtgtcaagtc agtagtaccc
     4381 atggttcatc tttcatggaa tatggcacga aatatcaaag tctcagatcc aaagcttttt
     4441 gaaatgatta aatattgtct cctcaagatt ctaaagcatt gtcagacttt gagagaagct
     4501 cttgttgcag cagggaaaga ggttttgtgg catgggagga taaatgatga accagctcct
     4561 tactgtagca tctgtgaggt ggaagttttt aatttacttt ttgtcactaa tgaaagcaat
     4621 tctcaaaaga catatatagt acattgtcag aactgtgcac gaaaaacaag tggaaatttg
     4681 gaaaattttg tagtgctaga acaatacaaa atggaggacc tgattcaagt ctatgaccaa
     4741 tttacattag ctccttcgtt atcttctgca tctaccggta agcctatccc taaccctctc
     4801 ctcggtctcg attctacgtg aggatccgcc cctctccctc ccccccccct aacgttactg
     4861 gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat
     4921 tgccgtcttt tggcaatgtg agatctagac ccagctttct tgtacaaagt tggcattata
     4981 agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc
     5041 attatttgcc atccagctgc agctctggcc cgtgtctcaa aatctctgat gttacattgc
     5101 acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac
     5161 aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat
     5221 ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac
     5281 aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg
     5341 tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat
     5401 gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac
     5461 tgcgatcccc ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa
     5521 tattgttgat gcgctggcag tgtccctgcg ccggttgcat tcgattcctg tttgtaattg
     5581 tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg
     5641 tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg
     5701 gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt
     5761 ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg
     5821 agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt
     5881 ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa
     5941 taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt
     6001 gtaacattat tcagattggg ccccgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6061 aggatcttc
//
