LOCUS       sequence-310586-        5668 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express gene of interest under CMV promoter. WPRE has been removed 
            to increase the cloning capacity..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5668)
  TITLE     lenti vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDH-CMV4
FEATURES             Location/Qualifiers
     source          1..5668
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        16..242
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             243..423
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    470..595
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1088..1321
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1506..1550
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1817..1933
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2023..2226
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2177..2197
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2223..2247
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     LTR             2419..2652
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2724..2845
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2761..2780)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2815..2834
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2885..3020
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3053..3069)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3053..3069)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3066..3088)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3077..3093
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3101..3107,3108..3125,3126..3131))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3146..3167
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3284..3301)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3455..4043)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3535..3554)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4214..5005,5006..5074))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4837..4856
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5075..5179)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5247..5265
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5303..5325)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5425..5444
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5638..5660
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(5652..5668,1)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     join(5653..5668,1)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgccaagctg acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
       61 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag
      121 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac
      181 gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa
      241 acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
      301 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
      361 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
      421 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
      481 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
      541 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
      601 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
      661 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
      721 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
      781 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
      841 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
      901 gcaaaacaaa agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag
      961 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     1021 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     1081 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     1141 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     1201 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     1261 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     1321 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     1381 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     1441 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     1501 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     1561 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     1621 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     1681 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
     1741 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatctc
     1801 gacggtatcg gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     1861 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     1921 aattcaaaat tttatcgata ctagtattat gcccagtaca tgaccttatg ggactttcct
     1981 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     2041 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     2101 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     2161 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ttatataagc
     2221 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
     2281 catagaagat tctagagcta gggatccgct agccccgggg cggccgcgaa ttcgtcgacg
     2341 gcgcgccggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     2401 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagataa gatctgcttt
     2461 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     2521 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     2581 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     2641 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     2701 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     2761 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     2821 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     2881 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     2941 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     3001 aggaggcttt tttggaggcc tagacttttg cagagaccaa attcgtaatc atgtcatagc
     3061 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     3121 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     3181 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     3241 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     3301 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     3361 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     3421 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     3481 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     3541 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     3601 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     3661 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     3721 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     3781 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     3841 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     3901 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     3961 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     4021 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     4081 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     4141 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     4201 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     4261 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     4321 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     4381 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     4441 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     4501 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     4561 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     4621 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     4681 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     4741 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     4801 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     4861 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     4921 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     4981 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     5041 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     5101 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     5161 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     5221 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     5281 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     5341 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     5401 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     5461 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     5521 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     5581 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     5641 agtcacgacg ttgtaaaacg acggccag
//
