LOCUS       sequence-31059-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  TITLE     The Parkinson Disease-associated Leucine-rich Repeat Kinase 2 
            (LRRK2) Is a Dimer That Undergoes Intramolecular Autophosphorylation
  JOURNAL   J Biol Chem. 2008 Jun 13;283(24):16906-14.
  PUBMED    18397888
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2008-06-13"; volume: "283(24)"; pages: "16906-14"
COMMENT     Sequence Label: 2XMyc-LRRK2-WT
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        56..74
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      106..115
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             115..144
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             160..189
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    208..232
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tagcagagct ggtttagtga accgtcagat ccgctagcga ttacgccaag ctcgaaatta
       61 accctcacta aagggaacaa aagctggagc tccaccgcgg tggcggccgc catggagcag
      121 aaactcatct ctgaagagga tctgagcccg ggcggatccg agcagaaact catctctgaa
      181 gaggatctgc tgcaggaatt cgatatcaca agtttgtaca aaaaagcagg ctccgaattc
      241 gcccttatgg ctagtggcag ctgtcagggg tgcgaagagg acgaggaaac tctgaagaag
      301 ttgatagtca ggctgaacaa tgtccaggaa ggaaaacaga tagaaacgct ggtccaaatc
      361 ctggaggatc tgctggtgtt cacgtactcc gagcacgcct ccaagttatt tcaaggcaaa
      421 aatatccatg tgcctctgtt gatcgtcttg gactcctata tgagagtcgc gagtgtgcag
      481 caggtgggtt ggtcacttct gtgcaaatta atagaagtct gtccaggtac aatgcaaagc
      541 ttaatgggac cccaggatgt tggaaatgat tgggaagtcc ttggtgttca ccaattgatt
      601 cttaaaatgc taacagttca taatgccagt gtaaacttgt cagtgattgg actgaagacc
      661 ttagatctcc tcctaacttc aggtaaaatc accttgctga tattggatga agaaagtgat
      721 attttcatgt taatttttga tgccatgcac tcatttccag ccaatgatga agtccagaaa
      781 cttggatgca aagctttaca tgtgctgttt gagagagtct cagaggagca actgactgaa
      841 tttgttgaga acaaagatta tatg
//
