LOCUS       sequence-310599-        3774 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vitis GRF4-GIF1 in pDONR-zeo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Debernardi JM, Tricoli DM, Ercoli MF, Hayta S, Ronald P, Palatnik 
            JF, Dubcovsky J
  TITLE     A GRF-GIF chimeric protein improves the regeneration efficiency of 
            transgenic plants.
  JOURNAL   Nat Biotechnol. 2020 Oct 12. pii: 10.1038/s41587-020-0703-0. doi: 
            10.1038/s41587-020-0703-0.
  PUBMED    33046875
REFERENCE   2  (bases 1 to 3774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Oct 12. pii: 10.1038/s41587-020-0703-0. doi: 
            10.1038/s41587-020-0703-0."
COMMENT     Sequence Label: JD630
FEATURES             Location/Qualifiers
     source          1..3774
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2370..2469)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2486..2505)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2487..2505)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2510..2526)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2510..2526)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(2594..2968)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(2987..3034)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     rep_origin      3124..3712
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3613..3632
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agctgccacc atgaagcaaa gctttgtggg ggtttcttca caaacagagg ctgagacaag
      721 agccagagag agtgacacac agacacaccc acaaaggaac ctcagtcact gtccccaaaa
      781 acatcctcaa aacttgcaaa gaacccaaaa tggtgggtct gttggggggg catcaatggc
      841 aacagggatg atggtgaggt catcaccatt cacagtgtct cagtggcagg agctggagca
      901 tcaagctttg atctttaagt atctaatggc aggactaccc gtcccacctg atcttgtgct
      961 tcctattcag aagagctttg aatctatgtc ttctaggttc ttccaccatc ctaccatggg
     1021 ttactgttcc ttctatggga agaaggtgga cccggagcca ggtagatgcc ggaggactga
     1081 tggcaagaaa tggaggtgct ccaaagacgc ctacccggac tccaagtact gtgagcgcca
     1141 catgcaccgt ggccgcaacc gttcaagaaa gcctgtggaa tcacaaacta ctacacagtc
     1201 ttcttcgact gtgacttcac taactgtcac tggaggcagt ggcagtggag ccgggacctt
     1261 ccagaacctt cccctgcaaa acttcaataa cccccaaggc ggcccggggt ccggaaccaa
     1321 ccaatcccaa ttccatatgg agcccattcc ctatggcatt cctaataaag attacaggta
     1381 tcttcatgga cttaaacccg aggtaggcga acatagtttc ttctctgaag cttcaggaag
     1441 caacaggggt cttcagatgg actcgccttt ggacagcaca tggcctctga tgccatcacg
     1501 ggtctcatca ttccccccat caaaatcaag tgaaaactcc atcttgcaga gtgactaccc
     1561 ccagcattcg ttcttcagca gcgaatttgc gtcgggagag gttgtgaaac aggagggtca
     1621 gtctctccgt cctttctttg acgagtggcc taaaaccaga gactcctggt ccggccttga
     1681 ggaagagagg tccaaccaga cctcattctc cacaacccag ctctcaattt cgatcccaat
     1741 ggcctcctct gacttctcaa caaccagctc tcggtctccc cacgataacg cggccgctgc
     1801 catgcagccc atgatggcag cctattaccc cagcaacgtc accactgatc acattcagca
     1861 gtatcttgat gaaaacaagt cattgattct gaagattgtt gagagccaga attcaggaaa
     1921 attgactgaa tgtgcagaga accaggcaag actacagaga aacctcatgt acctggctgc
     1981 aatcgctgat tctcaacccc aaccacccac catgcatgct cagttccctc ctagtggcat
     2041 tgttcagcca ggagctcact acatgcagca ccaacaagct caacaaatga caccacagtc
     2101 gctcctggct gcacgctcct ccatgctgta ctcccaacaa ccattttcgg ccctgcaaca
     2161 acaacaagcc atccatagcc agcttggcat gggctctggt ggaagtgcag gacttcacat
     2221 gctgcaaagc gaggggagta atccaggagg caatggaaca ctggggactg atatgggaaa
     2281 tgcagaaggg cgaggaggga actcaggagg tcagggtggg gatggaggtg agactcttta
     2341 cttgaaagct gctgaagatg ggaattgacg ttcagctttc ttgtacaaag ttggcattat
     2401 aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
     2461 cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt
     2521 ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaat
     2581 aatatcatca tgatcagtcc tgctcctcgg ccacgaagtg cacgcagttg ccggccgggt
     2641 cgcgcagggc gaactcccgc ccccacggct gctcgccgat ctcggtcatg gccggcccgg
     2701 aggcgtcccg gaagttcgtg gacacgacct ccgaccactc ggcgtacagc tcgtccaggc
     2761 cgcgcaccca cacccaggcc agggtgttgt ccggcaccac ctggtcctgg accgcgctga
     2821 tgaacagggt cacgtcgtcc cggaccacac cggcgaagtc gtcctccacg aagtcccggg
     2881 agaacccgag ccggtcggtc cagaactcga ccgctccggc gacgtcgcgc gcggtgagca
     2941 ccggaacggc actggtcaac ttggccatgg tttagttcct caccttgtcg tattatacta
     3001 tgccgatata ctatgccgat gattaattgt caacacgtgc tgatcatgac caaaatccct
     3061 taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     3121 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3181 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3241 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     3301 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3361 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3421 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3481 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     3541 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     3601 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     3661 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     3721 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtt
//
