LOCUS       sequence-31062-e         382 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 382)
  TITLE     The Parkinson Disease-associated Leucine-rich Repeat Kinase 2 
            (LRRK2) Is a Dimer That Undergoes Intramolecular Autophosphorylation
  JOURNAL   J Biol Chem. 2008 Jun 13;283(24):16906-14.
  PUBMED    18397888
REFERENCE   2  (bases 1 to 382)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2008-06-13"; volume: "283(24)"; pages: "16906-14"
COMMENT     Sequence Label: 2XMyc-LRRK2-R1441C
FEATURES             Location/Qualifiers
     source          1..382
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        35..53
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      85..94
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             94..123
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             139..168
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    187..211
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ccgtcagatc cgctagcgat tacgccaagc tcgaaattaa ccctcactaa agggaacaaa
       61 agctggagct ccaccgcggt ggcggccgcc atggagcaga aactcatctc tgaagaggat
      121 ctgagcccgg gcggatccga gcagaaactc atctctgaag aggatctgct gcaggaattc
      181 gatatcacaa gtttgtacaa aaaagcaggc tccgaattcg cccttatggc tagtggcagc
      241 tgtcaggggt gcgaagagga cgaggaaact ctgaagaagt tgatagtcag gctgaacaat
      301 gtccaggaag gaaaacagat agaaacgctg gtccaaatcc tggaggatct gctggtgttc
      361 acgtactccg agcacgcctc ca
//
