LOCUS       sequence-31064-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  TITLE     The Parkinson Disease-associated Leucine-rich Repeat Kinase 2 
            (LRRK2) Is a Dimer That Undergoes Intramolecular Autophosphorylation
  JOURNAL   J Biol Chem. 2008 Jun 13;283(24):16906-14.
  PUBMED    18397888
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2008-06-13"; volume: "283(24)"; pages: "16906-14"
COMMENT     Sequence Label: 2XMyc-LRRK2-Y1699C
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        51..69
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      101..110
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             110..139
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             155..184
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    203..227
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agctggttta gtgaaccgtc aggatccgct agcgattacg ccaagctcga aattaaccct
       61 cactaaaggg aacaaaagct ggagctccac cgcggtggcg gccgccatgg agcagaaact
      121 catctctgaa gaggatctga gcccgggcgg atccgagcag aaactcatct ctgaagagga
      181 tctgctgcag gaattcgata tcacaagttt gtacaaaaaa gcaggctccg aattcgccct
      241 tatggctagt ggcagctgtc aggggtgcga agaggacgag gaaactctga agaagttgat
      301 agtcaggctg aacaatgtcc aggaaggaaa acagatagaa acgctggtcc aaatcctgga
      361 ggatctgctg gtgttcacgt actccgagca cgcctccaag ttatttcaag gcaaaaatat
      421 ccatgtgcct ctgttgatcg tcttggactc ctatatgaga gtcgcgagtg tgcagcaggt
      481 gggttggtca cttctgtgca aattaataga agtctgtcca ggtacaatgc aaagcttaat
      541 gggaccccag gatgttggaa atgattggga agtccttggt gttcaccaat tgattcttaa
      601 aatgctaaca gttcataatg ccagtgtaaa cttgtcagtg attggactga agaccttaga
      661 tctcctccta acttcaggta aaatcacctt gctgatattg gatgaagaaa gtgatatttt
      721 catgttaatt tttgatgcca tgcactcatt tccagccaat gatgaagtcc agaaacttgg
      781 atgcaaagct ttacatgtgc tgtttgagag agtctcagan gagcaactga ctgaatttgt
      841 tgagaacaaa gattatatga
//
