LOCUS       sequence-310652-       10589 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of PHKG1 (human) tagged with C-terminal 
            V5-miniTurbo for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10589)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-PHKG1-V5-miniTurbo_IDG-K
FEATURES             Location/Qualifiers
     source          1..10589
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    113..229
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    261..281
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        281..1492
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1329..1348
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    1493..1517
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2682..2706)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2708..2749
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2756..3526
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    complement(3532..3552)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    3581..4169
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3634..3654)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4052..4063)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4183..4682
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4218..4241)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4615..4634
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4703..5302
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4703..4722)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5199..5219
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5440..5673
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5745..5866
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5782..5801)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5836..5855
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5885..6020
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6040..6059)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6041..6059)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6069..6086)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6069..6085)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6078..6100)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6227..6682
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6314..6333)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6524..6545
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6708..6812
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6813..6881,6882..7673)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7844..8432
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8333..8352
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8586..8603
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8720..8741
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8756..8761,8762..8779,8780..8786)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8794..8810
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8799..8821
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8818..8834
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8818..8834
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8853..8873
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8855..8873
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8901..9127
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9128..9308
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9355..9480
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9973..10206
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10391..10435
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
       61 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta acttttaaaa
      121 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
      181 acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta tcgtccagtg
      241 tggtggaatt ctgcagatat caactttgta tagaaaagtt ggcctccgcg ccgggttttg
      301 gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga cgaagggcgc
      361 agcgagcgtc ctgatccttc cgcccggacg ctcaggacag cggcccgctg ctcataagac
      421 tcggccttag aaccccagta tcagcagaag gacattttag gacgggactt gggtgactct
      481 agggcactgg ttttctttcc agagagcgga acaggcgagg aaaagtagtc ccttctcggc
      541 gattctgcgg agggatctcc gtggggcggt gaacgccgat gattatataa ggacgcgccg
      601 ggtgtggcac agctagttcc gtcgcagccg ggatttgggt cgcggttctt gtttgtggat
      661 cgctgtgatc gtcacttggt gagtagcggg ctgctgggct ggccggggct ttcgtggccg
      721 ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc caagggctgt agtctgggtc
      781 cgcgagcaag gttgccctga actgggggtt ggggggagcg cagcaaaatg gcggctgttc
      841 ccgagtcttg aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa acaaggtggg
      901 gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg ggaaagctct
      961 tattcgggtg agatgggctg gggcaccatc tggggaccct gacgtgaagt ttgtcactga
     1021 ctggagaact cggtttgtcg tctgttgcgg gggcggcagt tatggcggtg ccgttgggca
     1081 gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac ccgttctgtt
     1141 ggcttataat gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt ttctccgtcg
     1201 caggacgcag ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc cggacctctg
     1261 gtgaggggag ggataagtga ggcgtcagtt tctttggtcg gttttatgta cctatcttct
     1321 taagtagctg aagctccggt tttgaactat gcgctcgggg ttggcgagtg tgttttgtga
     1381 agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt ttcagtgtta
     1441 gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag acacaagttt
     1501 gtacaaaaaa gttggcacca tgacccggga cgaggcactg ccggactctc attctgcaca
     1561 ggacttctat gagaattatg agcccaaaga gatcctgggc aggggcgtta gcagtgtggt
     1621 caggcgatgc atccacaagc ccacgagcca ggagtacgcc gtgaaggtca tcgacgtcac
     1681 cggtggaggc agcttcagcc cggaggaggt gcgggagctg cgagaagcca cgctgaagga
     1741 ggtggacatc ctgcgcaagg tctcagggca ccccaacatc atacagctga aggacactta
     1801 tgagaccaac actttcttct tcttggtgtt tgacctgatg aagagagggg agctctttga
     1861 ctacctcact gagaaggtca ccttgagtga gaaggaaacc agaaagatca tgcgagctct
     1921 gctggaggtg atctgcacct tgcacaaact caacatcgtg caccgggacc tgaagcccga
     1981 gaacattctc ttggatgaca acatgaacat caagctcaca gactttggct tttcctgcca
     2041 gctggagccg ggagagaggc tgcgagaggt ctgtgggacc cccagttacc tggcccctga
     2101 gattatcgag tgctccatga atgaggacca cccgggctac gggaaagagg tggacatgtg
     2161 gagcactggc gtcatcatgt acacgctgct ggccggctcc ccgcccttct ggcaccggaa
     2221 gcagatgctg atgctgagga tgatcatgag cggcaactac cagtttggct cgcccgagtg
     2281 ggatgattac tcggacaccg tgaaggacct ggtctcccga ttcctggtgg tgcaacccca
     2341 gaaccgctac acagcggaag aggccttggc acaccccttc ttccagcagt acttggtgga
     2401 ggaagtgcgg cacttcagcc cccgggggaa gttcaaggtg atcgctctga ccgtgctggc
     2461 ttcagtgcgg atctactacc agtaccgccg ggtgaagcct gtgacccggg agatcgtcat
     2521 ccgagacccc tatgccctcc ggcctctgcg ccggctcatc gacgcctacg ctttccgaat
     2581 ctatggccac tgggtgaaga aggggcagca gcagaaccgg gcagcccttt tcgagaacac
     2641 acccaaggcc gtgctcctct ccctggccga ggaggactac ttgccaactt tcttgtacaa
     2701 agtgggcggc aagcccatcc ccaaccccct gctgggcctg gacagcaccg ctagcatccc
     2761 gctgctgaac gctaaacaga ttctgggaca gctggacggc gggagcgtgg cagtcctgcc
     2821 tgtggtcgac tccaccaatc agtacctgct ggatcgaatc ggcgagctga agagtgggga
     2881 tgcttgcatt gcagaatatc agcaggcagg gagaggaagc agagggagga aatggttctc
     2941 tccttttgga gctaacctgt acctgagtat gttttggcgc ctgaagcggg gaccagcagc
     3001 aatcggcctg ggcccggtca tcggaattgt catggcagaa gcgctgcgaa agctgggagc
     3061 agacaaggtg cgagtcaaat ggcccaatga cctgtatctg caggatagaa agctggcagg
     3121 catcctggtg gagctggccg gaataacagg cgatgctgca cagatcgtca ttggcgccgg
     3181 gattaacgtg gctatgaggc gcgtggagga aagcgtggtc aatcagggct ggatcacact
     3241 gcaggaagca gggattaacc tggacaggaa tactctggcc gctatgctga tccgagagct
     3301 gcgggcagcc ctggaactgt tcgagcagga aggcctggct ccatatctgt cacggtggga
     3361 gaagctggat aacttcatca atagacccgt gaagctgatc attggggaca aagagatttt
     3421 cgggattagc cgggggattg ataaacaggg agccctgctg ctggaacagg acggagttat
     3481 caaaccctgg atgggcggag aaatcagtct gcggtctgcc gaaaagtaag ccaactttat
     3541 tatacatagt tgatatccag cacagtggcg gccgctcgac aatcaacctc tggattacaa
     3601 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     3661 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     3721 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     3781 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     3841 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     3901 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     3961 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
     4021 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     4081 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     4141 tcggatctcc ctttgggccg cctccccgcc tggaattcta ccgggtaggg gaggcgcttt
     4201 tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa
     4261 gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct
     4321 ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc
     4381 cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc
     4441 agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc
     4501 agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc
     4561 tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct
     4621 gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt
     4681 cgacctgcag cccaagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     4741 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     4801 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     4861 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     4921 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     4981 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     5041 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     5101 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     5161 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     5221 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     5281 gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag
     5341 cgcacgaccc catgcatctc gagggcccgg tacctttaag accaatgact tacaaggcag
     5401 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta gctcactccc
     5461 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     5521 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     5581 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     5641 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     5701 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     5761 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     5821 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     5881 agctatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     5941 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     6001 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
     6061 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     6121 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     6181 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     6241 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     6301 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     6361 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     6421 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     6481 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     6541 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     6601 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     6661 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     6721 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     6781 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     6841 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     6901 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     6961 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     7021 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     7081 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     7141 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     7201 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     7261 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     7321 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     7381 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     7441 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     7501 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     7561 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     7621 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     7681 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     7741 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     7801 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     7861 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     7921 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     7981 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     8041 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     8101 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     8161 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     8221 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gyggacaggt
     8281 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     8341 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     8401 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     8461 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     8521 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     8581 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     8641 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     8701 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     8761 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     8821 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
     8881 agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt
     8941 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt
     9001 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
     9061 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata
     9121 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     9181 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     9241 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     9301 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     9361 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     9421 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     9481 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     9541 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     9601 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     9661 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     9721 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     9781 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     9841 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     9901 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     9961 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
    10021 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
    10081 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
    10141 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
    10201 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
    10261 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
    10321 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
    10381 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
    10441 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
    10501 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
    10561 atattcacca ttatcgtttc agacccacc
//
