LOCUS       sequence-310653-       10255 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes a polyspecific aminoacyl-tRNA synthetase fused to a modified
            releasing factor and four copies of amber suppression tRNAs for 
            unnatural amino acid incorporation in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10255)
  AUTHORS   Chen Z, Zhang S, Li X, Ai HW
  TITLE     A high-performance genetically encoded fluorescent biosensor for 
            imaging physiological peroxynitrite.
  JOURNAL   Cell Chem Biol. 2021 Jan 27. pii: S2451-9456(21)00013-1. doi: 
            10.1016/j.chembiol.2021.01.013.
  PUBMED    33581056
REFERENCE   2  (bases 1 to 10255)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Chem 
            Biol. 2021 Jan 27. pii: S2451-9456(21)00013-1. doi: 
            10.1016/j.chembiol.2021.01.013."
COMMENT     Sequence Label: pMAH-POLY-eRF1(E55D)
FEATURES             Location/Qualifiers
     source          1..10255
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(111..201)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     promoter        complement(313..561)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        complement(850..940)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     promoter        complement(1052..1300)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     enhancer        1559..1862
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1863..2066
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2017..2037
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2063..2087
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2297..2315
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2297..2314
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2328..2347
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      2380..2389
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3682..3735
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    5087..5675
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5140..5160)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5558..5569)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5695..5712)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5701..5925
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5971..6399
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6058..6077)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6268..6289
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6408..6428)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6413..6742
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6593..6728
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6655..6674
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6791..7816
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6791..7816
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    7946..8067
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7983..8002)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8037..8056
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8116..8132)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8116..8132)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8129..8151)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8140..8156
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8164..8170,8171..8188,8189..8194))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8209..8230
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8347..8364)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8518..9106)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8598..8617)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9277..10068,10069..10137))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9900..9919
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10138..10242)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctcgactcgc tagtgttcca aaaaatggtg ggggacggat tcgaaccgcc gaacccaaag
       61 ggagcggatt tagagtccgc ggcgtttggc cacttcgcta ccccaccaga gtggtctcat
      121 acagaactta taagattccc aaatccaaag acatttcacg tttatggtga tttcccagaa
      181 cacatagcga catgcaaata tgaattgatg actgcctgat ggagaaaaaa tggtggggga
      241 cggattcgaa ccgccgaacc caaagggagc ggatttagag tccgcggcgt ttggccactt
      301 cgctacccca ccggtgtttc gtcctttcca caagatatat aaagccaaga aatcgaaata
      361 ctttcaagtt acggtaagca tatgatagtc cattttaaaa cataatttta aaactgcaaa
      421 ctacccaaga aattattact ttctacgtca cgtattttgt actaatatct ttgtgtttac
      481 agtcaaatta attctaatta tctctctaac agccttgtat cgtatatgca aatatgaagg
      541 aatcatggga aataggccct cttcctgccc gaccttcgcg atgtacgggc cagatatacg
      601 ccaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
      661 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      721 aatatgaccg ccatgttggc tcgactcgct agtgttccaa aaaatggtgg gggacggatt
      781 cgaaccgccg aacccaaagg gagcggattt agagtccgcg gcgtttggcc acttcgctac
      841 cccaccagag tggtctcata cagaacttat aagattccca aatccaaaga catttcacgt
      901 ttatggtgat ttcccagaac acatagcgac atgcaaatat gaattgatga ctgcctgatg
      961 gagaaaaaat ggtgggggac ggattcgaac cgccgaaccc aaagggagcg gatttagagt
     1021 ccgcggcgtt tggccacttc gctaccccac cggtgtttcg tcctttccac aagatatata
     1081 aagccaagaa atcgaaatac tttcaagtta cggtaagcat atgatagtcc attttaaaac
     1141 ataattttaa aactgcaaac tacccaagaa attattactt tctacgtcac gtattttgta
     1201 ctaatatctt tgtgtttaca gtcaaattaa ttctaattat ctctctaaca gccttgtatc
     1261 gtatatgcaa atatgaagga atcatgggaa ataggccctc ttcctgcccg accttcgcga
     1321 tgtacgggcc agatatacgc caatattggc cattagccat attattcatt ggttatatag
     1381 cataaatcaa tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca
     1441 tttatattgg ctcatgtcca atatgaccgc catgttggct cgagcattga ttattgacta
     1501 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     1561 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     1621 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     1681 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     1741 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     1801 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     1861 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
     1921 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     1981 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
     2041 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc
     2101 atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc cgcggccggg
     2161 aacggtgcat tggaacgcgg attccccgtg ccaagagtga cgtaagtacc gcctatagag
     2221 tctataggcc cacccccttg gcttcgttag aacgcggcta caattaatac ataaccttat
     2281 gtatcataca catacgattt aggtgacact atagaataac atccactttg cctttctctc
     2341 cacaggtgtc cactcccagg tccaactgca cggaagcttg ccaccatggc aagcagtaac
     2401 ttgattaaac aattgcaaga gcgggggctg gtagcccagg tgacggacga ggaagcgcta
     2461 gcagagcgac tggcgcaagg cccgatcgca ctcatttgtg gcttcgatcc taccgctgac
     2521 agcttgcatt tggggcatct tgttccattg ttatgcctga aacgcttcca gcaggcgggc
     2581 cacaagccgg ttgcgctggt aggcggcgcg acgggtctga ttggcgaccc gagcttcaaa
     2641 gctgccgagc gtaagctgaa caccgaagaa actgttcagg agtgggtgga caaaatccgt
     2701 aagcaggttg ccccgttcct cgatttcgac tgtggagaaa actctgctat cgcggccaat
     2761 aattatgact ggttcggcaa tatgaatgtg ctgaccttcc tgcgcgatat tggcaaacac
     2821 ttctccgtta accagatgat caacaaagaa gcggttaagc agcgtctcaa ccgtgaagat
     2881 caggggattt cgttcactga gttttcctac aacctgctgc agggttattc gatggcctgt
     2941 gctaacaaac agtacggtgt ggtgctgcaa attggtggtt ctgaccagtg gggtaacatc
     3001 acttctggta tcgacctgac ccgtcgtctg catcagaatc aggtgtttgg cctgaccgtt
     3061 ccgctgatca ctaaagcaga tggcaccaaa tttggtaaaa ctgaaggcgg cgcagtctgg
     3121 ttggatccga agaaaaccag cccgtacaaa ttctaccagt tctggatcaa cactgcgcgt
     3181 gccgacgttt accgcttcct gaagttcttc acctttatga gcattgaaga gatcaacgcc
     3241 ctggaagaag aagataaaaa cagcggtaaa gcaccgcgcg cccagtatgt actggcggag
     3301 caggtgactc gtctggttca cggtgaagaa ggtttacagg cggcaaaacg tattaccgaa
     3361 tgcctgttca gcggttcttt gagtgcgctg agtgaagcgg acttcgaaca gctggcgcag
     3421 gacggcgtac cgatggttga gatggaaaag ggcgcagacc tgatgcaggc actggtcgat
     3481 tctgaactgc aaccttcccg tggtcaggca cgtaaaacta tcgcctccaa tgccatcacc
     3541 attaacggtg aaaaacagtc cgatcctgaa tacttcttta aagaagaaga tcgtctgttt
     3601 ggtcgtttta ccttactgcg tcgcggtaaa aagaattact gtctgatttg ctggaaaggg
     3661 cccgttggat ccggctccgg cgagggcagg ggaagtctac taacatgcgg ggacgtggag
     3721 gaaaatcccg gcccagctag catggcggac gaccccagtg ctgccgacag gaacgtggag
     3781 atctggaaga tcaagaagct cattaagagc ttggaggcgg cccgcggcaa tggcaccagc
     3841 atgatatcat tgatcattcc tcccaaagac cagatttcac gagtggcaaa aatgttagcg
     3901 gatgattttg gaactgcatc taacattaag tcacgagtaa accgcctttc agtcctggga
     3961 gccattacat ctgtacaaca aagactcaaa ctttataaca aagtacctcc aaatggtctg
     4021 gttgtatact gtggaacaat tgtaacagaa gaaggaaagg aaaagaaagt caacattgac
     4081 tttgaacctt tcaaaccaat taatacgtca ttgtatttgt gtgacaacaa attccataca
     4141 gaggctctta cagcactact ttcagatgat agcaagtttg gattcattgt aatagatggt
     4201 agtggtgcac tttttggcac actccaagga aacacaagag aagtcctgca caaattcact
     4261 gtggatctcc caaagaaaca cggtagagga ggtcagtcag ccttgcgttt tgcccgttta
     4321 agaatggaaa agcgacataa ctatgttcgg aaagtagcag agactgctgt gcagctgttt
     4381 atttctggga acaaagtgaa tgtggctggt ctagttttag ctggatccgc tgactttaaa
     4441 actgaactaa gtcaatctga tatgtttgat cagaggttac aatcaaaagt tttaaaatta
     4501 gttgatatat cctatggtgg tgaaaatgga ttcaaccaag ctattgagtt atctactgaa
     4561 gtcctctcca acgtgaaatt cattcaagag aagaaattaa taggacgata ctttgatgaa
     4621 atcagccagg acacgggcaa gtactgtttt ggcgttgaag atacactaaa ggctttggaa
     4681 atgggagctg tagaaattct aatagtctat gaaaatctgg atataatgag atatgttctt
     4741 cattgccaag gcacagaaga ggagaaaatt ctctatctaa ctccagagca agaaaaggat
     4801 aaatctcatt tcacagacaa agagaccgga caggaacatg agcttatcga gagcatgccc
     4861 ctgttggaat ggtttgctaa caactataaa aaatttggag ctacgttgga aattgtcaca
     4921 gataaatcac aagaagggtc tcagtttgtg aaaggatttg gtggaattgg aggtatcttg
     4981 cggtaccgag tagatttcca gggaatggaa taccaaggag gagacgatga attttttgac
     5041 cttgatgact actaatgaga attcgttccg gagtcgtcga ctcgacaatc aacctctgga
     5101 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     5161 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     5221 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     5281 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     5341 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     5401 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     5461 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     5521 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     5581 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg catcttcgcc ttcgccctca
     5641 gacgagtcgg atctcccttt gggccgcctc cccgcgttta aacccgctga tcagcctcga
     5701 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     5761 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     5821 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     5881 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     5941 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     6001 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     6061 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     6121 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     6181 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     6241 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     6301 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     6361 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     6421 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     6481 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     6541 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     6601 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     6661 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     6721 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     6781 tcagcacgtg atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga
     6841 aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt
     6901 cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt
     6961 ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt
     7021 gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg
     7081 tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga
     7141 ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg
     7201 accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc
     7261 ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc
     7321 tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc
     7381 ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg
     7441 gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc
     7501 gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc
     7561 aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag
     7621 cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt
     7681 ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg
     7741 gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc
     7801 gagggcaaag gaatagcacg tgctacgaga tttcgattcc accgccgcct tctatgaaag
     7861 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     7921 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     7981 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     8041 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     8101 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     8161 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     8221 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     8281 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     8341 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     8401 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     8461 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8521 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8581 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8641 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     8701 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     8761 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     8821 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     8881 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     8941 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     9001 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     9061 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     9121 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     9181 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     9241 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     9301 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     9361 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     9421 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9481 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9541 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     9601 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     9661 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     9721 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     9781 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     9841 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     9901 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     9961 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    10021 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    10081 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    10141 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    10201 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccga
//
