LOCUS       sequence-310655-       12636 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Over expression of Rictor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12636)
  AUTHORS   Xu ZX, Tan JW, Xu H, Hill CJ, Ostrovskaya O, Martemyanov KA, Xu B
  TITLE     Caspase-2 promotes AMPA receptor internalization and cognitive 
            flexibility via mTORC2-AKT-GSK3beta signaling.
  JOURNAL   Nat Commun. 2019 Aug 9;10(1):3622. doi: 10.1038/s41467-019-11575-1.
  PUBMED    31399584
REFERENCE   2  (bases 1 to 12636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-08-9"; volume: "10(1):3622. doi"; pages: " 
            10.1038/s41467-019-11575-1"
COMMENT     Sequence Label: Rictor-Myc
FEATURES             Location/Qualifiers
     source          1..12636
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1212
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1049..1068
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             1247..1264
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(2636..2647)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             6395..6424
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    6462..7050
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6515..6535)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6933..6944)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7141..7374
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7446..7567
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7483..7502)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7537..7556
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7607..7742
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7762..7781)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7763..7781)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7791..7808)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7791..7807)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7800..7822)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7949..8404
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8036..8055)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8246..8267
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8430..8534
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8535..8603,8604..9395)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8753..8772)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9566..10154
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10055..10074
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10308..10325
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10442..10463
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10478..10483,10484..10501,10502..10508)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10516..10532
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10521..10543
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10540..10556
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10540..10556
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10575..10595
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10577..10595
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10623..10849
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10850..11030
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11077..11202
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11695..11928
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12113..12157
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12455..12572
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg
       61 ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag
      121 cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag
      181 gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg
      241 aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat
      301 gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt
      361 cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct
      421 ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc
      481 caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg
      541 cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga
      601 ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt
      661 cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct
      721 gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt
      781 tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc
      841 gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg
      901 cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat
      961 cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg
     1021 gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg
     1081 ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca
     1141 atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct
     1201 tttttgttag acgaagcttg ggctgcaggt ccgatccacc ggtatgcacc accaccacca
     1261 ccacgccagc gcggcgatcg gccgcggccg ctctctgaag aacctccgaa tacgagggcg
     1321 gaatgacagc ggcgaggaga acgtcccgct cgatctgacc cgagaacctt ctgataactt
     1381 gagggagatt ctccaaaatg tggccaaatt gcaaggagta tcaaatatga gaaagctggg
     1441 ccatctgaat aacttcacta agcttctttg cgatattggc catagtgaag aaaaactggg
     1501 ctttaattat gaagacatca taatttgttt gcgcttagct ttattaaatg aggcaaaaga
     1561 agtaagagca gccgggttgc gagcgctgcg ctatctcatc caagattcca gtattctcca
     1621 gaaggtgcta aaactgaaag tggactattt aatagctagg tgcattgaca ttcagcagag
     1681 caacgaggtg gagaggacac aagcccttcg cttagtcaga aagatgatta ctgtgaatgc
     1741 ttccttgttt cctagttctg tggccaactc attaattgcg gttggaaatg atgggcttca
     1801 ggaacgagac agaatggtcc gggcttgcat cgccatcatc tgtgaactag cacttcagaa
     1861 cccagaggtg gtagcccttc gaggaggact aaacaccata ctgaaaaatg tgattgattg
     1921 ccaattaagt cgaataaatg aggctctcat tactacaatt ttgcaccttc ttaatcatcc
     1981 aaagacccgg cagtatgtgc gagctgatgt agaattagag cgaattttag caccctatac
     2041 tgatttccac tacagacaca gtccagatac ggctgaagga cagctcaaag aagacagaga
     2101 agcacgattt ctagccagta agatgggaat cattgcgaca ttccgctcgt gggcaggtat
     2161 tattaattta tgtaagcctg gaaattctgg gatacagtct ctaattggag tactttgcat
     2221 accgaatatg gaaataaggc gaggtctgct cgaagtgctc tatgacatct ttcgtctccc
     2281 tctgcccgtt gtgacggatg agttcataga agccctgctc agtgtagatc caggaagatt
     2341 ccaggacagt tggaggcttt ccgatggctt tgtggcagct gaggcaaaaa ctattcttcc
     2401 ccatcgtgct agatccaggc cagacctcat ggacaattat ttggctctga tactctctgc
     2461 atttattcgt aatggacttt tagagggctt ggtcgaggtg ataacgaaca gtgacgacca
     2521 catctcagtg agggcgacca tccttttagg agagctcctg catatggcaa acaccatcct
     2581 gcctcactcg catagccacc atctacactg cttgccaacc ctcatgaaca tggctgcgtc
     2641 cttcgatatt cccaaggaaa agaggctgcg agccagtgca gccctgaact gtttgaaccg
     2701 cttccatgag atgaagaagc gaggacctaa gccctacagc cttcatttag atcacatcat
     2761 tcagaaagca atcgcaactc accacaagcg ggatcagtat cttcgagttc agaaagatat
     2821 atttgttctt aaggatacag aggaagctct tttaataaac cttagagaca gccaagtcct
     2881 tcagcataaa gagaatcttg actgggattg gaatctgatt gggaccatcc ttaagtggcc
     2941 aaatgtaaat ctaagaaact ataaagatga gcagttgcac aggtttgtgc gcagacttct
     3001 ttacttttac aagcccagca gcaaactgta cgctagtctg gatctggact tggccaagtc
     3061 caagcagctc acagttgttg gttgtcagtt tacagaattt ctgctcgagt ctgaagagga
     3121 tgggcaagga tacttagaag atctcgtgaa agatattgtt cagtggctca atgcttcatc
     3181 tggagtgaaa cctgagagaa gccttcagaa taatggctta ctgaccaccc ttagtcaaca
     3241 ctacttctta tttatcggaa cactctcttg tcatcctcat ggagtcaaaa tgctggaaaa
     3301 gtgcagtgtc tttcagtgtc tccttaatct ttgttccttg aaaaaccaag atcatctgat
     3361 aaaactcact gtttccagcc tagactatag cagagatgga ttagccagag tcattctttc
     3421 aaaaatcctc acggcagcca cagatgcttg cagactgtat gcaacaaaac atttaagggt
     3481 tttattgaga gctaacgttg aattcttcaa caattgggga attgagttac tagtaactca
     3541 gctacatgat aaaaacaaaa caatttcttc tgaagcgctg gatattcttg atgaagcttg
     3601 tgaagacaag gccaatcttc atgctcttat tcagatgaag ccagccttat cccaccttgg
     3661 agacaagggc ttgcttctac tgctgagatt tctttccatt ccgaaaggat tttcttacct
     3721 gaatgaaagg ggttatgttg caaaacagtt ggaaaagtgg cacaaggaat ataattcaaa
     3781 gtatgttgac ttgattgagg aacaacttaa tgaagccctc actacataca ggaagcctat
     3841 tgatggtgat aactacgttc gtcgcagtaa tcaaagacta cagcgacctc atgtctacct
     3901 gcccgtacac ctttatggac aactggtaca ccacaaaaca ggctgccatt tactggaagt
     3961 acagagtatc atcacagagc tctgtcacaa tgttcgcact ccagacttgg acaaatggga
     4021 agacattaag aagctgaaag cctctctttg ggccttggga aatattggct catcgaattg
     4081 ggggcttaat ttgctacagg aagaaaatgt gatcccagat atactaaaac ttgcaaaaca
     4141 gtgtgaggtc ctttccatcc gagggacctg tgtatatgtt cttgggctca tagctaaaac
     4201 caagcaaggc tgtgatattt tgaaatgtca cagctgggat tctgtaaggc acagtcgcaa
     4261 gcatctatgg ccagtggttc cagatgatgt ggagcagctc tgcaatgagc tctcatctgt
     4321 cccaagcacc ctcagcttga actctgagtc gaccagctct agacataaca gtgagagtga
     4381 gtctgcacca tcaagcatgt tcatgctgga ggacgaccgc ttcggcagca cctccaccag
     4441 cacatttttc cttgacatca atgaagatgc tgagccagca ttttatgacc gacctggacc
     4501 cataaaagat aaaaattcat ttcccttttt tggctccagc aaacttgtaa agaatcgtat
     4561 cttaaattca cttactttgc ctactaaaaa acaccgcagt agcagtgatc caaaaggagg
     4621 aaaactgtca tctgaaaata agacaagcaa ccggcgaatc agaacactta cggagcccag
     4681 tgttgacttg aatcacagcg aagacttcac aagctcctca gcacagaaat cattacagct
     4741 agagccttcc ttcgttggga ataagcacct tgaagacgcg ggcagcactc caagtattgg
     4801 agagaatgac ctcaaattcc ccaagagttt tggtaccgag actcacagag aaaacacaag
     4861 ccgagaaagg ttggttgtgg aaggctcagc aagctcccac atcaagatcc gcagccagag
     4921 ctttaacacg gacactacga cgagtggcat cagctccatg agctcgagcc cctcccgaga
     4981 gacagtcgct gtcgacccca cagctatgga cacggactgt ggaagtctga gtaccgtggt
     5041 aagtactaaa actgttaaga cgagccacta tttgacacca cagtctaacc atctgtctct
     5101 ctccaaatcg aactcagtgt ccctggtgcc accaggttct tctcacaccc ttcctagaag
     5161 agcccagtcc cttaaagcgc cctccattgc aacaataaaa agtctagcag attgcaactt
     5221 cagctacaca agctctagag atgcctttgg ctatgctaca ttgaaaaggt tacagcagca
     5281 aagaatgcac ccgtccttgt ctcactcgga agctttggcg tcgccggcaa aagatgtgct
     5341 ttttaccgac accatcacca tgaaggccaa cagctttgaa tccagactga cgccaagcag
     5401 gtttatgaaa gctttaagtt atgcgtcatt agataaagaa gatttactaa gtcctattaa
     5461 tcacaatacc ctccagcgct cctcctcagt taggtctatg gtgtccagtg ctacatatgg
     5521 aggttccgat gactacattg gacttgctct tccagtagac atcaatgaca tcttccagat
     5581 aaaggatgtt ccttattttc aatcaaagca cgtgcctcca ccagatgacc gaggtgcaag
     5641 aatgttttcc catgatggag caggtctttc atctggagcc ggtggtctcg taaagaactc
     5701 ttttcacttg ctgcggcagc agatgagtct cacagaaata atgaactctg tccattcgga
     5761 cgcctccctg ttcttagaga gcacagaaga cactgggctg caggagcaca cggatgacaa
     5821 ttgcctttac tgcgtctgca ttgaactcct gggcttccag cccagtaacc agctaagttc
     5881 catctgcagt cactccgatc ttcaagatat tccgtactcc gattggtgcg agcagacgat
     5941 ccacaacccc ttagaagtgg ttccctctaa gttctcgggg atttctggct gcagcgatgg
     6001 cgcatctcag gaagaaggct cagccagcag caccaaaagc actgagctgt tactgggtgt
     6061 taaaaccatt ccagatgaca cacccatgtg ccggatcctg cttcgtaagg aagtgctaag
     6121 attagttgtc aatttgagta gttcagtttc caccaagtgt cacgagaccg ggcttctgac
     6181 cattaaagag aagtatcctc aaacatttga tgacatatgc ctttattctg aagtttctca
     6241 tctgttgtca cactgcacat ttagacttca gtgtcggcgg ttcatacaag agttatttca
     6301 ggatgtacag tttttacaaa tgcatgaaga agcagaggct gtgttggcaa ttccaccaat
     6361 tcagcctata gttgatgagt ctgctgaatc cgccgaacaa aaactcatct cagaagagga
     6421 tctgaatagc taggaattcg ttccggagtc gtcgactcga caatcaacct ctggattaca
     6481 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     6541 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     6601 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     6661 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     6721 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     6781 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     6841 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     6901 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     6961 cccgcggcct gctgccggct ctgcggcctc ttccgcatct tcgccttcgc cctcagacga
     7021 gtcggatctc cctttgggcc gcctccccgc ctggaattaa ttcgagctcg gtacctttaa
     7081 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     7141 tggaagggct acgtaactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     7201 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     7261 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     7321 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     7381 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     7441 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     7501 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     7561 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     7621 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     7681 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     7741 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     7801 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     7861 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     7921 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     7981 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     8041 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     8101 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     8161 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     8221 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     8281 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     8341 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     8401 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     8461 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     8521 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     8581 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     8641 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     8701 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     8761 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     8821 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     8881 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     8941 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     9001 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     9061 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     9121 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     9181 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     9241 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     9301 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     9361 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     9421 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     9481 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     9541 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     9601 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     9661 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     9721 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     9781 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     9841 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     9901 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     9961 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
    10021 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    10081 ctgtcgggtt tcgccacctc tcacttgagc gtcgattttt gtgatgctcg tcaggggggc
    10141 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    10201 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    10261 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
    10321 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
    10381 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
    10441 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
    10501 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
    10561 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
    10621 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
    10681 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
    10741 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
    10801 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
    10861 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
    10921 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
    10981 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
    11041 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
    11101 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
    11161 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
    11221 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
    11281 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
    11341 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
    11401 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
    11461 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
    11521 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
    11581 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
    11641 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
    11701 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
    11761 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
    11821 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
    11881 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
    11941 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
    12001 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
    12061 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
    12121 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
    12181 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
    12241 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
    12301 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
    12361 tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc
    12421 aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
    12481 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
    12541 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
    12601 cagtttggtt aattaacccg tgtcggctcc agatct
//
