LOCUS       sequence-310660-        6202 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cell body-targeted GCaMP6f, under synapsin promoter: GCaMP6f 
            followed by a linker, and the EE-RR coiled-coil motif 
            (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6202)
  AUTHORS   Shemesh OA, Linghu C, Piatkevich KD, Goodwin D, Celiker OT, Gritton 
            HJ, Romano MF, Gao R, Yu CJ, Tseng HA, Bensussen S, Narayan S, Yang 
            CT, Freifeld L, Siciliano CA, Gupta I, Wang J, Pak N, Yoon YG, 
            Ullmann JFP, Guner-Ataman B, Noamany H, Sheinkopf ZR, Park WM, Asano
            S, Keating AE, Trimmer JS, Reimer J, Tolias AS, Bear MF, Tye KM, Han
            X, Ahrens MB, Boyden ES
  TITLE     Precision Calcium Imaging of Dense Neural Populations via a 
            Cell-Body-Targeted Calcium Indicator.
  JOURNAL   Neuron. 2020 Jun 26. pii: S0896-6273(20)30398-6. doi: 
            10.1016/j.neuron.2020.05.029.
  PUBMED    32592656
REFERENCE   2  (bases 1 to 6202)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2020 Jun 26. pii: S0896-6273(20)30398-6. doi: 
            10.1016/j.neuron.2020.05.029."
COMMENT     Sequence Label: pAAV-Syn-SomaGCaMP6f2
FEATURES             Location/Qualifiers
     source          1..6202
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     polyA_signal    186..234
                     /note="synthetic polyadenylation signal"
     misc_feature    248..339
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     288..307
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        375..822
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      831..840
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(837..845,846..863,864..866,867..899,900..902,903..926,
                     927..953,954..1010,1011..1016,1017..1286,1287..1304,
                     1305..1739,1740..1745,1746..2186)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6f
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEEFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      933..942
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1223..1244
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1350..1371)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1611..1630)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    2565..3153
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2618..2638)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3036..3047)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3186..3203)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3192..3399
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   3408..3537
                     /label=AAV2 ITR
     rep_origin      3630..4085
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3717..3736)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3927..3948
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4152..4171)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4271..4293
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4331..4349)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4417..4521
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4522..4590,4591..5382)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4740..4759)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5553..6141
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6042..6061
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttctaga aatattaagg tacgggaggt acttggagcg
      181 gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga
      241 tagtactaac atacgctctc catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg
      301 ctgtccccag tgcaagtgca ggtgccagaa catttctcta ttctagactc gagctgcaga
      361 gggccctgcg tatgagtgca agtgggtttt aggaccagga tgaggcgggg tgggggtgcc
      421 tacctgacga ccgaccccga cccactggac aagcacccaa cccccattcc ccaaattgcg
      481 catcccctat cagagagggg gaggggaaac aggatgcggc gaggcgcgtg cgcactgcca
      541 gcttcagcac cgcggacagt gccttcgccc ccgcctggcg gcgcgcgcca ccgccgcctc
      601 agcactgaag gcgcgctgac gtcactcgcc ggtcccccgc aaactcccct tcccggccac
      661 cttggtcgcg tccgcgccgc cgccggccca gccggaccgc accacgcgag gcgcgagata
      721 ggggggcacg ggcgcgacca tctgcgctgc ggcgccggcg actcagcgct gcctcagtct
      781 gcggtgggca gcggaggagt cgtgtcgtgc ctgagagcgc agtcggatcc gccaccatgg
      841 gttctcatca tcatcatcat catggtatgg cttccatgac tggtggacag caaatgggtc
      901 gggatctgta cgacgatgac gataaggatc tcgccaccat ggtcgactca tcacgtcgta
      961 agtggaataa gacaggtcac gcagtcagag ctataggtcg gctgagttca ctcgagaacg
     1021 tctatatcaa ggccgacaag cagaagaacg gcatcaaggc gaacttcaag atccgccaca
     1081 acatcgagga cggcggcgtg cagctcgcct accactacca gcagaacacc cccatcggcg
     1141 acggccccgt gctgctgccc gacaaccact acctgagcgt gcagtccaaa ctttcgaaag
     1201 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     1261 ctctcggcat ggacgagctg tacaagggcg gaaccggagg gagcatggtg agcaagggcg
     1321 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     1381 acaagttcag cgtgtccggc gagggtgagg gcgatgccac ctacggcaag ctgaccctga
     1441 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     1501 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     1561 agtccgccat gcccgaaggc tacatccagg agcgcaccat cttcttcaag gacgacggca
     1621 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     1681 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaacc
     1741 tgccggacca actgactgaa gagcagatcg cagaatttaa agaggagttc tccctatttg
     1801 acaaggacgg ggatgggaca ataacaacca aggagctggg gacggtgatg cggtctctgg
     1861 ggcagaaccc cacagaagca gagctgcagg acatgatcaa tgaagtagat gccgacggtg
     1921 acggcacaat cgacttccct gagttcctga caatgatggc aagaaaaatg aaatacaggg
     1981 acacggaaga agaaattaga gaagcgttcg gtgtgtttga taaggatggc aatggctaca
     2041 tcagtgcagc agagcttcgc cacgtgatga caaaccttgg agagaagtta acagatgaag
     2101 aggttgatga aatgatcagg gaagcagaca tcgatgggga tggtcaggta aactacgaag
     2161 agtttgtaca aatgatgaca gcgaaggagc tcgggggcag cggtgggtct ggagggactg
     2221 ggggtagcgg tggtagcggt gggacagggg gaagcggcgg tagcggtggg acactggaaa
     2281 tcgaggccgc tttcctggag caggagaaca ccgccctgga gacagaggtg gctgagctgg
     2341 agcaggaggt gcagcggctg gagaacatcg tgtctcagta cgagaccaga tacggaccac
     2401 tgggatccct ggagatccgg gccgcttttc tgaggagacg gaacaccgcc ctgaggacaa
     2461 gggtggctga gctgaggcag agagtgcagc ggctgcgcaa catcgtgagc cagtacgaga
     2521 cacgctacgg acctctgtaa gaattcgata tcaagcttat cgataatcaa cctctggatt
     2581 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     2641 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     2701 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     2761 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     2821 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     2881 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     2941 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     3001 ggattctgcg cgggacgccc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     3061 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     3121 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacta gagctcgctg
     3181 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     3241 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     3301 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     3361 gggggaggat tgggaagaca atagcaggca tgctggggag agatctgagg aacccctagt
     3421 gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa
     3481 ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagaga
     3541 gggagccccc ccctgcagcc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     3601 aacagttgcg cagcctgaat ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc
     3661 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     3721 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     3781 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     3841 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     3901 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     3961 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     4021 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     4081 aatttcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
     4141 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     4201 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     4261 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     4321 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     4381 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     4441 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     4501 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     4561 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     4621 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     4681 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     4741 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     4801 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     4861 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     4921 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     4981 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     5041 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     5101 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     5161 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     5221 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     5281 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     5341 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     5401 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     5461 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     5521 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     5581 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     5641 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     5701 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     5761 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     5821 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     5881 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     5941 gagctatgag aaagcgccac gcttcctgaa gggagaaagg cggacaggta tccggtaagc
     6001 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     6061 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     6121 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     6181 tgctggcctt ttgctcacat gt
//
