LOCUS       sequence-310667-       14257 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cell body-targeted GCaMP7f, under HuC promoter: GCaMP7f followed by 
            a linker, and the EE-RR coiled coil motif (GCaMP7f-27-EE-RR). As 
            bright as conventional GCaMP7f..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14257)
  AUTHORS   Shemesh OA, Linghu C, Piatkevich KD, Goodwin D, Celiker OT, Gritton 
            HJ, Romano MF, Gao R, Yu CJ, Tseng HA, Bensussen S, Narayan S, Yang 
            CT, Freifeld L, Siciliano CA, Gupta I, Wang J, Pak N, Yoon YG, 
            Ullmann JFP, Guner-Ataman B, Noamany H, Sheinkopf ZR, Park WM, Asano
            S, Keating AE, Trimmer JS, Reimer J, Tolias AS, Bear MF, Tye KM, Han
            X, Ahrens MB, Boyden ES
  TITLE     Precision Calcium Imaging of Dense Neural Populations via a 
            Cell-Body-Targeted Calcium Indicator.
  JOURNAL   Neuron. 2020 Jun 26. pii: S0896-6273(20)30398-6. doi: 
            10.1016/j.neuron.2020.05.029.
  PUBMED    32592656
REFERENCE   2  (bases 1 to 14257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2020 Jun 26. pii: S0896-6273(20)30398-6. doi: 
            10.1016/j.neuron.2020.05.029."
COMMENT     Sequence Label: elavl3:SomaGCaMP7f
FEATURES             Location/Qualifiers
     source          1..14257
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(163..184)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(533..544,545..707)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     regulatory      9482..9491
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9488..9496,9497..9514,9515..9517,9518..9550,9551..9553,
                     9554..9577,9578..9604,9605..9661,9662..9667,9668..9937,
                     9938..9955,9956..10390,10391..10396,10397..10837)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6f
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKELFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYTDSEEEIREAFRVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      9584..9593
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     9874..9895
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(10001..10022)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(10262..10281)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    11233..11367
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11257..11276)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     11311..11330
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(11806..11993,11994..12005)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(12039..12055)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12039..12055)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(12092..12112)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(12092..12110)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12131..12147)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12131..12147)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12144..12166)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12155..12171
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12179..12185,12186..12203,12204..12209))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12224..12245
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12362..12379)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12533..13121)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12613..12632)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13292..14083,14084..14152))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13915..13934
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14153..14257)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg
       61 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
      121 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
      181 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
      241 ggcccacgct ggctaagaac tcatcagcct ccccggtcca tctacccacg taccaatgca
      301 ccaattggcc acaatgacgg ctactacatg gtgccattcc ttcctcttta taggaatgga
      361 gactacctcc tgtccaacaa tgctcttgga tacgagtacg cctacctgtt ggacccaggt
      421 cattgcacaa caccagaaat gccctctgat ctgcaaaaga cgtgaatatc tgttcagaca
      481 cccatatcca ctctgttcca cacaggtcag aggtttgtcc aggagttctt gacagaggtg
      541 taaaaagtac tcaaaaattt tactcaagtg aaagtacaag tacttaggga aaattttact
      601 caattaaaag taaaagtatc tggctagaat cttacttgag taaaagtaaa aaagtactcc
      661 attaaaattg tacttgagta ttaaggaagt aaaagtaaaa gcaagaaaga aaactagaga
      721 ttcttgttta agcttcccgg gtgtcgacga attctcgagc tctattccta aagaccttgg
      781 gtgaccaaaa tcttatttta ataaataaaa ctgtttatta aaactttttt gtttcaaaga
      841 accatatgta tagtgaaatt tataaaaata tcaattttta aaaagctggt gtactcattt
      901 atgttatgaa ctctaaaacc atatactgac tgcaagtgat gatgtataga gtgatgttta
      961 cgagtaaaca tatttagttg tatacatcct actgagcaca ttttgatgta tgaaataaca
     1021 ttacaagctt tatccaaatt aagccatttt aaaacactgc caattgaaaa tacaaatcct
     1081 ggaaaaaatc gtctttagcg cagtcatttg agccatccta atccgttacc tcagaccata
     1141 ataagaaggg ataacactag ctgtagcaat ggaacacatc tgtttcacac aatcatatct
     1201 cctgcgccgg tgctaagcag attcagcgtg atcataacat gctttccact cataaatgta
     1261 aatttacaat ttgcacatgt aaaacagaca cttttgagat attggataaa aaaacaagag
     1321 tatattgctt agtttcatcc accagtcatc cccacagcgt ttggaaggcc ataaaaagtg
     1381 tctaaaatca atgatcattg aaagagcaca agagagactc ttacgctgta atgccactgg
     1441 ggacaaaagt gacagtctct taatgggctc ttctggaggg gctcctgaac attaaaaatt
     1501 atcagcgaaa ttaccgaaag agcttcaagc aactggcatg cttgatcctc tgcgtcgggg
     1561 cggtgaatag gtgcttcaga tgccctctta cccacgggct ggattcagct gccccgctac
     1621 cagcggagac cccctaatga gcctctgcaa ttaagtttat tcatgttaag tgtgaacggg
     1681 gtgcgtgcgg aactgtgggc agctaacaga cctgggttct ttgtgccaca agtgctgcct
     1741 ttattcggct cacaaagcag aaaacaacac ccgcacctat aatggcgccc tcggctgggt
     1801 ctaagaaacg tggcgagttg acagagcaga gtgggcgggg ttaagacaga ctgacagcgg
     1861 gacccatctc catcctctta ttaacgctta acgagtgcct tcctcatgca atattcatcg
     1921 ccactaatat catccaagct ctgagctgag ctggccactt atgtaaggca attatgtaaa
     1981 atatcagaca gggcccacac tcagaatctg actggggtag agacgcggga cgagaaccga
     2041 gagcaagaac tgaaagtgaa agtgaccact aaagggagga gaggacagag gggcaggatg
     2101 tgtcaagatt accagagaac acttggccag aaatgcgcaa ccattggagc tctccggatt
     2161 acccaaaggt taacgagttt gaacgcctct gcccactcgc ccatctctga tggtttccca
     2221 agaactcctc aagcaaaata tatataattg tgtgtattat gcacagacac gagaaaatgc
     2281 tgtttttctg atctgcatta cagcacattt gcccgccaac gacaatacca cccactcggt
     2341 acctcgctga ctcctgatgc ctgatacctg cgcggtgact gtctacaatc tgcataatca
     2401 agagaagttg tgttgaagac gagcgccaca caaccgtttc cacaaggtca cccaaggccg
     2461 gtgcagatgt aggtgaggtc tccataaaca gactgaaata aacacatcct ccgctgggaa
     2521 caacaacccc ctcacgcctc atgcatttcc ataagcctac atgcatctct tccaacttat
     2581 ggagactcgc acctaccaac atccgcacaa caaagatata cagagcgcgc tccctcaggt
     2641 caaggccctg tgggggtctg tgcagaaata ggtcatttgt cacacatcaa gtcctggggc
     2701 aggagatgca ttatagatga gaccaaacag cctgtctcgg tgagctctac ccactccctg
     2761 agactagaaa tgggggaagg gagcttgaga taacaaccgc tgcaatcact gtgtcgatgt
     2821 ttaatatcag caccaaccgg gaacaataag gagatgggtg cattcatgtt cacatcttac
     2881 cagtcaagta tcatcgaacc ggcttgataa ccacacctcg tgtaatagct gagcagatag
     2941 ttgtcatttt aaagcgttgg cctttgtcga ttatgtaatg cgcacattca acacatggta
     3001 atatagaaac ggttatgtcg aggttgtttt gtccagagat gaccttcaca cagttacagc
     3061 cgctctgcat ccacacaaat ggaggactta atcgtggact gcattcttag aaatgatcta
     3121 caaagacaaa taatgtgaaa tcaagaaagg acaaaattta agtaagggga tgagggagag
     3181 agagaacgag gggcaaggag aaagcatggc tcctgtcttt ttctgcaccc atctgttcgg
     3241 agtgcaggtg gagctctatt cactcagctc tgcatgtgtg tttggggggg gcaggaagaa
     3301 agggagggca aaaggaagag tggagagatg gtgggggctg gagggatggg gggttctcgg
     3361 tgatctctcc tgaaggggat aatgggagag cagcgctttg caatggctgc catgtagtac
     3421 cctccctgca caattagcca atcagcagca agctctgcca gccagaagga cacataaaag
     3481 aagaacattg cagcagaggc acagaaggag cctgcgagga gctgggaaat acacacacaa
     3541 cagcagaacc acaacaccct cccctggaca caccctactg gggatcactg cttttctttt
     3601 tttctgaacc atcgcccacg ccacacggag agaaatctct ctctcatcat catcctgaag
     3661 aaaaccccct tatcctcatt ttcacactgc tgaggaaaac ctacaatcgc acgggctgag
     3721 atttcctggc gaagactgtc ctttttcctt tttctttttt tttcttttcc tttggaaact
     3781 gacatttgca tttctccatt ccaagccacg gcgtaataat atctgcaatc cagcctgaag
     3841 acctgcaaat cgaaggacct agatcactat catctttgta cgtcaaggat cctggttact
     3901 gtacgtataa cctttctttc ttttgctctg accaatatga aacactaaaa tcgattcgag
     3961 cagcctctca gcatcaatta cagtgcgtga aaaacattca aatagaggca gcaaatatta
     4021 catgtgaaaa tacaggctgg ctaaatccaa gctaatttag aaatgtggtc aaaacgcata
     4081 ctggcacgtc taatcgcgga ttcagtaaac aagattaacg attagcccag tgtataagtc
     4141 atatgatacc ggcatgcgcg agagcatcgc tacacccgag ctggcttcat tttcggagga
     4201 aaatcaaaac attgctttct cctgccgtgc gaaccattcg tcataaaccg taatacgcaa
     4261 catacattta ttactacatc cgttaattag cgataattag ccgttattaa caaagagcgc
     4321 tgaggaattc ctctcaaata gcggaggtgc ggcggaggca gaggggcgta aaagggcaca
     4381 tgcgtgcctg gctcaaaaaa ggatgctcca gactgaaact cagagacaaa acacgatgcc
     4441 tcggaggctg agagcccatc gagaggaaag gcaaagaaaa gggtcgagtc actcagaggg
     4501 ggaggggaga tattgtgcat ccattggttt gaattgaagc aggcagaata aaaatccttg
     4561 cgattatgct tcgtgagaga tcgcgagaga ggaaaaggct aaatgccacg tatgcaaatg
     4621 gataaatgtc atctatttct tcgcgagtag acattttgtg gacaacgaga gtgtaaataa
     4681 gcgatatgag gggaatacaa aggccttgag ggaaagcgtt ttgggcgtgt cttgtcgaaa
     4741 agaagacagc gatggcacgc gctcagaacc accatcttca cgtagacaat ccgcgtgaaa
     4801 acaataacaa aactgctcat tttaacacga gcaggtgttt gaaaccaccc aaaaaagatt
     4861 gtctaaaata ttaagattaa ttaatgacgt ttaaatgtcc aggcctgata taaatgaagc
     4921 gtgtagtaag agtaatcgcc tttgtttctt ttcagagata cgttttcaga gattcaggga
     4981 attatatctt caataaaata atgtttgatt tcattatttt aaagcttttt aagaagtaaa
     5041 aatacacatt attcatttac attttatttt atttaaaggc ctatggtctt gaaaataata
     5101 agtggaatat ataggctata tattcgtcta aggacagccg gtgttatttt atagtgacct
     5161 cagacaaaag ccgagacaaa acgaggcgct tgcctttcat tttaatcctt aatagacggt
     5221 gtgatgaatg aatgaatgaa tgaataaatg aatgaatgac gggcccttcg gcatgttgtt
     5281 ccgcagtcct ccgtgagcac gagtctctac cgcagtgcca agcacataaa accgttgagt
     5341 cagataacct tctccaggcg cgtatacaca attcaaggac aagcacatca caactataca
     5401 caacacaaca caaccgccta aacgcacgag ctcatcaatc gcaatttaaa ggcatgtcag
     5461 tcaaagaaaa aggcggcgtt atttaaaacc aatgttcaac aatatttgta tgctgactag
     5521 cccgggcgcg cacgctataa gatcacaagg ccttgttttt tccctttatt caaaatatca
     5581 ttaattatta caggtcataa aacaaaacaa tgcgaattac aatgatttaa agactattga
     5641 tttaacaata atcagttaat tatagaataa tattataata ttttacatac aaatgaaaga
     5701 tgacattatt tgatgacatt gtaattaaaa tatatgcaat atatttttac gtttttatta
     5761 attaggctat tactattatt ataattatca ttatttttat tattattatt attattatta
     5821 aatagtcttg cgaaataaga gtgaaggcag ttgcaatgtt cattgttccc ggagagtccc
     5881 gcagcctctg gcctcaacac aaagcgctac tgtttacatt aatattcata gtgctgccat
     5941 ggtctctgta gaggaggaac gggtggctct tttgttcgct agtgtcaata cgtttattca
     6001 gcaaaaagca gctcggattt gtgaatgccc aacaagcaca cgcgcactcc aatcaaagga
     6061 cgagaggata acgattttaa acgattcgca aacgtgttta tttgtaaaga cgacatcaag
     6121 agcttaatgt ccacttgaaa agaaataaat atgcccgcgg cgccaaggat attttgaatg
     6181 tagtcgttca tggtgagcca ctcccgaatt cagcactctg gagagcgacc aaagaggaat
     6241 agcggaatag caactacttg agccatttct ctccgtttga tggcgttcat gattaaaaat
     6301 ataatcacgt gattttttat acatatataa atatatacat aaagtaacgc tttccttttg
     6361 agaatacata aatatttatt cgcgtgataa atcaatgatc ttatatttat ttgcacgacc
     6421 gaaattagcc aaattcacca attaaaaaaa aaataagcca acaaaaaaga agggtttatc
     6481 cgttcaattt tgacttgtgt cctgttgttt tacagctgcc actgtgatcc cttaagctgc
     6541 agtgagagtg tggctaatgc cttttgttta agataatcct gtaatctgtt accgaaacgg
     6601 cctattgaca agccggcatt cacatttcag tcaggaggcc cgtccagaca tgtacattta
     6661 tgaattacga atgataaaat taagatctgc attaggcgtc agaaatgtca cgaacaccca
     6721 ttcatttccc acagccaata atggatcatg ctgggaagcg ctatcctcgc agtctcaaaa
     6781 ttaatgttga catgttgcgt agagctacag attagatcag ccatatgctt tatgtgttta
     6841 cctcagcgga tcttcagcaa gatgtgttta ttttaagaaa atggcttcct cgctgcctgc
     6901 acaggggcat taaggaagtg acgtgagcgt cctcacagtc aaattacttt atctcatgct
     6961 gctttcaggc ctctgcttat tttattatta ttattttaaa ttagggggaa tcacggtggc
     7021 gttgtgggta gggtagcgcg atcaccttac agcaagaagg tcgctggttc gagctctggc
     7081 tgggtcagtt ggcatttctg tgtggagttt aaattgaatt gaaattgaat aaactaaatt
     7141 ggccctagtg tatgtgtgtg aatgtaagtg tgcatgggtg tttcccagtg ttgggtttga
     7201 ctggaagagc atccgcttcg taaaacatat gctggataag ttggcggttc atttcactgt
     7261 ggtgaccgct gattaataaa gggactaagc tgaaaatgaa atgaattaat acattttaaa
     7321 tgagcttttg ctgacatgtt tatgattaca aatagtgaaa ttgtatgtta atttacgata
     7381 agtgcattgt tctaaattca tttttatgta gcagctggtt tttcttccat ttgtgtcatt
     7441 caacagaaca tttttctaaa aaagtgaact taaattttct cctccctctg acaaggcttg
     7501 atggtaattt cagccgactc taattacaga ctcactaaaa ggaatcggtg cagtcattta
     7561 tctttatcag cgcgctggtg ggaaggtaat aggttttgct tatgagttgt ttgcgtgcga
     7621 gtctgggtcc ttgtgtaggc gatgctgatg cactgcatta aatattgagg gagaagtcgc
     7681 tcaaaaatag caaagtacag gctggggcag gaacgtcaaa gcccgctcag tggccacgtt
     7741 tcttgtttcg taagtcctat ttttaatagc gataattcag gctcagtaga aggaactcca
     7801 gcactataag cagtctctcg cctgtctcgg cgagttatgt attttgggat gaagtgctct
     7861 tccacctcta cctctgctgt gctggcgcca gtatctgggc tgtaagatag tgtacgtgtc
     7921 agtctgtgtg gccgaagagc ctgtgtgttg tgtaactcac tacattcgtc gctgtggatg
     7981 gctgacttgc catggggagg tttacttcag ttcacgaatc acattcgcca cgatcttgtc
     8041 actttgtgtc aagactcgct ctctgtgtgt caacagctct gctgtttgag gttgaaattc
     8101 tgaacttgag atatatccca ggatatatta gggctactgc caacggaaaa agcaaatggt
     8161 gccatggcca tttgattgat caaacaaaca cccttttgct tgggtgacag tgaaatgtca
     8221 gatccatgtt tgtactgctt taataacttg cctctctgag ctttctcctt gtcagtgtca
     8281 atttgcctga gatataaatt ccggttgccc cgaaagctta ttttattaaa aaaaagctaa
     8341 catgcactca tacaacgtgc gcacatacat gtgaggcttg ctaaaagtca agaagcaagc
     8401 caatagcaag agtcgtcaga ctgctgttac ctgggcaacc tggttagagg taaggctcaa
     8461 ggcaagaata aatcattcca ctgaagagac acagcgttac agcgagatga gagcaagagg
     8521 cgatagtgaa gggagaacaa aagaacagcc tacccacact agtgcagatc tacctgtttg
     8581 agctactgat tgttcagttt gattagatat cagacaattc tgctatctcc aattgcattg
     8641 ctgcatgaat tcctagaaat cattttaatc accaaaaact agtagggaca cttgtggcgt
     8701 atatgattct gcctgttgat tgtggggatc caacagaaag atcatgaatg cattgttaat
     8761 taagtcggtg agacaccctg ttccacccta ggggccgttc tggggaaaga gtgctttcag
     8821 tcagctaaag atgactaata tgtgaacata tattactaca catgccaatt tgtacattct
     8881 ggatagttac cagccctggg aaaacatcga aaaaaataaa tcaacataga taaaattggc
     8941 aaatcccttc tggtacgatg cagagtgctt gctctgagaa cttgtgcatt gtgacagaaa
     9001 gcgaccgtta aaaactgaaa gtagcatcta caacctgcat acatctgttc tttgaaaatc
     9061 ccctgtaggc caaattaagc atcacgctgt gctccacagc aacacggatg aaccgaaaac
     9121 ccagacacaa acgcacacac ttttcacata aacaaggttt agttatgcta gctatgcttt
     9181 ttcgtcctcc ctttgtcatc agcggcgagt gacgtaacac agtttgaccc tggacagcgt
     9241 aaaggggggg ttgggaatga gtgaaaggac tgtgacatct gcagggccag gggctgatgg
     9301 agagagctga agtgagggag gtcagaggag gaggaagggt gggatattgt cctcctcagg
     9361 ccctatccct ctcagacgca ttgattttta acctctcagc gagaatgcca agcattacac
     9421 ccctcaaatt atggttcctc acccaacctg ttatattttc cacctgcaga taattaggcg
     9481 cgccaccatg ggttctcatc atcatcatca tcatggtatg gcttccatga ctggtggaca
     9541 gcaaatgggt cgggatctgt acgacgatga cgataaggat ctcgccacca tggtcgactc
     9601 atcacgtcgt aagtggaata agacaggtca cgcagtcaga gctataggtc ggctgagttc
     9661 actggagaac gtctatatca aggccgacaa gcagaagaac ggcatcaagg cgaacttcaa
     9721 gatccgccac aacatcgagg acggcggcgt gcagctcgcc taccactacc agcagaacac
     9781 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagcg tgcagtccaa
     9841 actttcgaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     9901 cgccgggatc actctcggca tggacgagct gtacaagggc ggaaccggag ggagcatggt
     9961 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
    10021 cgtaaacggc cacaagttca gcgtgtccgg cgagggtgag ggcgatgcca cctacggcaa
    10081 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
    10141 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
    10201 cgacttcttc aagtccgcca tgcccgaagg ctacatccag gagcgcacca tcttcttcaa
    10261 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
    10321 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
    10381 ggagtacaac ctgccggacc aactgactga agagcagatc gcagaattta aagagctgtt
    10441 ctccctattt gacaaggacg gggatgggac aataacaacc aaggagctgg ggacggtgat
    10501 gcggtctctg gggcagaacc ccacagaagc agagctgcag gacatgatca atgaagtaga
    10561 tgccgacggt gacggcacaa tcgacttccc tgagttcctg acaatgatgg caagaaaaat
    10621 gaaatacaca gacagtgaag aagaaattag agaagcgttc cgtgtgtttg ataaggatgg
    10681 caatggctac atcagtgcag cagagcttcg ccacgtgatg acaaaccttg gagagaagtt
    10741 aacagatgaa gaggttgatg aaatgatcag ggaagcagac atcgatgggg atggtcaggt
    10801 aaactacgaa gagtttgtac aaatgatgac agcgaaggga ggttctggag gttctggtgg
    10861 tactggtggt tctggaggat ctggtggtac aggaggtagc ggaggttctg gtggtaccct
    10921 ggaaatcgag gccgctttcc tggagcagga gaacaccgcc ctggagacag aggtggctga
    10981 gctggagcag gaggtgcaga gactggagaa catcgtgtct cagtacgaga ccagatacgg
    11041 accactggga tccctggaga tcagagccgc ttttctgagg agaagaaaca ccgccctgag
    11101 gacaagggtg gctgagctga ggcagagagt gcagagactg cgcaacatcg tgagccagta
    11161 cgagacacgc tacggacctc tgtaagttta aacggtggat cctggccgtc gttttactgt
    11221 accatcgatg atgatccaga catgataaga tacattgatg agtttggaca aaccacaact
    11281 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
    11341 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
    11401 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg
    11461 gctgattatg atcctctaga tcagatctgc gaagatacgg ccacgggtgc tcttgatcct
    11521 gtggctgatt ttggactgtg ctgctcgcag ctgctgatga atcacatact tcctccattt
    11581 tcttccactg attgactgtt ataatttccc taatttccag gtcaaggtgc tgtgcattgt
    11641 ggtaatagat gtgacatgac gtcacttcca aaggaccaat gaacatgtct gaccaatttc
    11701 atataatgtg aaaacgattt tcataggcag aataaataac atttaaatta aactgggcat
    11761 cagcgcaatt caattggttt ggtaatagca agggaaaata gaatgaagtg atctccaaaa
    11821 aataagtact ttttgactgt aaataaaatt gtaaggagta aaaagtactt ttttttctaa
    11881 aaaaatgtaa ttaagtaaaa gtaaaagtat tgatttttaa ttgtactcaa gtaaagtaaa
    11941 aatccccaaa aataatactt aagtacagta atcaagtaaa attactcaag tactttacac
    12001 ctctggttct tgacccccta ccttcagcaa gcccagcaga tccactagtt ctagagcggc
    12061 cgccaccgcg gtggagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg
    12121 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
    12181 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
    12241 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
    12301 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
    12361 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
    12421 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
    12481 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
    12541 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
    12601 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
    12661 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
    12721 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
    12781 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
    12841 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
    12901 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
    12961 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
    13021 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    13081 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
    13141 ctacggggtc tgacgctcag tggaacgaaa actcacgtta aggcattttg gtcatgagat
    13201 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
    13261 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
    13321 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
    13381 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
    13441 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
    13501 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
    13561 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
    13621 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    13681 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
    13741 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
    13801 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    13861 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    13921 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    13981 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    14041 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    14101 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    14161 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    14221 aatgtattta gaaaaataaa caaatagggg ttccgcg
//
