LOCUS       sequence-310670-        9434 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cDNA of NPM-ALK in the MSE backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9434)
  AUTHORS   Shoumariyeh K, Schneider N, Poggio T, Veratti P, Ehrenfeld S, 
            Redhaber DM, Khan R, Pfeifer D, Klingeberg C, Kreutmair S, Rudelius 
            M, Quintanilla-Martinez L, Fend F, Illert AL, Duyster J, Miething C
  TITLE     A novel conditional NPM-ALK-driven model of CD30+ T-cell lymphoma 
            mediated by a translational stop cassette.
  JOURNAL   Oncogene. 2020 Feb;39(9):1904-1913. doi: 10.1038/s41388-019-1058-1. 
            Epub 2019 Nov 21.
  PUBMED    31754210
REFERENCE   2  (bases 1 to 9434)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41388-019-1058-1"; journalName: "Oncogene"; date: 
            "2020-02"; volume: "39"; issue: "9"; pages: "1904-1913"
COMMENT     Sequence Label: MSCV-Stop-NPM-ALK-MSNA-IRES-EGFP (MSNAIE)
FEATURES             Location/Qualifiers
     source          1..9434
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..517
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    581..922
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             989..1405
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1335..1357
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1373..1389
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     protein_bind    complement(1456..1489)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             1503..2180
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(1683..1703)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     2095..2118
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    complement(2210..2243)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    4427..4994
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4444..4996
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4588..4605)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4815..4834
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5008..5010,5011..5013,5014..5727)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5053..5074)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5314..5333)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5661..5682
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5829..6343
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    6505..6521
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6529..6535,6536..6553,6554..6559))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6574..6595
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6712..6729)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6883..7471)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6963..6982)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7642..8433,8434..8502))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8265..8284
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8503..8607)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8675..8693
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8731..8753)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8853..8872
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9066..9088
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9216..9235
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
       61 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
      121 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      181 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
      241 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
      301 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
      361 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa
      421 taaagcctct tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct
      481 cagattgatt gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga
      541 gacccctgcc cagggaccac cgaccccccc gccgggaggt aagctggcca gcggtcgttt
      601 cgtgtctgtc tctgtctttg tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct
      661 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg
      721 aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc
      781 gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg
      841 agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg
      901 aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac
      961 tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac
     1021 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
     1081 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
     1141 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
     1201 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
     1261 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
     1321 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
     1381 agccctcact ccttctctag gcgccggaat tagatctctc gaggttaaac gggccctcta
     1441 gactcgagcg gccgcataac ttcgtatagc atacattata cgaagttatt ctagacacaa
     1501 ccatggcctc ctccgagaac gtcatcaccg agttcatgcg cttcaaggtg cgcatggagg
     1561 gcaccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     1621 gccacaacac cgtgaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca
     1681 tcctgtcccc ccagttccag tacggctcca aggtgtacgt gaagcacccc gccgacatcc
     1741 ccgactacaa gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     1801 aggacggcgg cgtggcgacc gtgacccagg actcctccct gcaggacggc tgcttcatct
     1861 acaaggtgaa gttcatcggc gtgaacttcc cctccgacgg ccccgtgatg cagaagaaga
     1921 ccatgggctg ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg
     1981 agacccacaa ggccctgaag ctgaaggacg gcggccacta cctggtggag ttcaagtcta
     2041 tctacatggc caagaagccc gtgcagctgc ccggctacta ctacgtggac gccaagctgg
     2101 acatcacctc ccacaacgag gactacacca tcgtggagca gtacgagcgc accgagggcc
     2161 gccaccacct gttcctgtag agctcgcgac tctagactga gtgagtgaca taacttcgta
     2221 tagcatacat tatacgaagt tatgcggccg ccactgtgct ggatatctgc agaattccac
     2281 cacactggac tagtggatcc gagctcggta ccaagcttgg ggggcgcctt ctctcctacc
     2341 taagtgcgtg ccgccacccg atggaagatt cgatggacat ggacatgagc cccctgaggc
     2401 cccagaacta tcttttcggt tgtgaactaa aggccgacaa agattatcac tttaaggtgg
     2461 ataatgatga aaatgagcac cagttatctt taagaacggt cagtttaggg gctggtgcaa
     2521 aggatgagtt gcacattgtt gaagcagagg caatgaatta cgaaggcagt ccaattaaag
     2581 taacactggc aactttgaaa atgtctgtac agccaacggt ttcccttggg ggctttgaaa
     2641 taacaccacc agtggtctta aggttgaagt gtggttcagg gccagtgcat attagtggac
     2701 agcacttagt agtgtaccgc cggaagcacc aggagctgca agccatgcag atggagctgc
     2761 agagccctga gtacaagctg agcaagctcc gcacctcgac catcatgacc gactacaacc
     2821 ccaactactg ctttgctggc aagacctcct ccatcagtga cctgaaggag gtgccgcgga
     2881 aaaacatcac cctcattcgg ggtctgggcc atggcgcctt tggggaggtg tatgaaggcc
     2941 aggtgtccgg aatgcccaac gacccaagcc ccctgcaagt ggctgtgaag acgctgcctg
     3001 aagtgtgctc tgaacaggac gaactggatt tcctcatgga agccctgatc atcagcaaat
     3061 tcaaccacca gaacattgtt cgctgcattg gggtgagcct gcaatccctg ccccggttca
     3121 tcctgctgga gctcatggcg gggggagacc tcaagtcctt cctccgagag acccgccctc
     3181 gcccgagcca gccctcctcc ctggccatgc tggaccttct gcacgtggct cgggacattg
     3241 cctgtggctg tcagtatttg gaggaaaacc acttcatcca ccgagacatt gctgccagaa
     3301 actgcctctt gacctgtcca ggccctggaa gagtggccaa gattggagac ttcgggatgg
     3361 cccgagacat ctacagggcg agctactata gaaagggagg ctgtgccatg ctgccagtta
     3421 agtggatgcc cccagaggcc ttcatggaag gaatattcac ttctaaaaca gacacatggt
     3481 cctttggagt gctgctatgg gaaatctttt ctcttggata tatgccatac cccagcaaaa
     3541 gcaaccagga agttctggag tttgtcacca gtggaggccg gatggaccca cccaagaact
     3601 gccctgggcc tgtataccgg ataatgactc agtgctggca acatcagcct gaagacaggc
     3661 ccaactttgc catcattttg gagaggattg aatactgcac ccaggacccg gatgtaatca
     3721 acaccgcttt gccgatagaa tatggtccac ttgtggaaga ggaagagaaa gtgcctgtga
     3781 ggcccaagga ccctgagggg gttcctcctc tcctggtctc tcaacaggca aaacgggagg
     3841 aggagcgcag cccagctgcc ccaccacctc tgcctaccac ctcctctggc aaggctgcaa
     3901 agaaacccac agctgcagag gtctctgttc gagtccctag agggccggcc gtggaagggg
     3961 gacacgtgaa tatggcattc tctcagtcca accctccttc ggagttgcac aaggtccacg
     4021 gatccagaaa caagcccacc agcttgtgga acccaacgta cggctcctgg tttacagaga
     4081 aacccaccaa aaagaataat cctatagcaa agaaggagcc acacgacagg ggtaacctgg
     4141 ggctggaggg aagctgtact gtcccaccta acgttgcaac tgggagactt ccgggggcct
     4201 cactgctcct agagccctct tcgctgactg ccaatatgaa ggaggtacct ctgttcaggc
     4261 tacgtcactt cccttgtggg aatgtcaatt acggctacca gcaacagggc ttgcccttag
     4321 aagccgctac tgcccctgga gctggtcatt acgaggatac cattctgaaa agcaagaata
     4381 gcatgaacca gcctgggccc tgatcccaag cttaagttta acgaattccg cccccccccc
     4441 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat
     4501 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct
     4561 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg
     4621 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc
     4681 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
     4741 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
     4801 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
     4861 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt
     4921 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
     4981 aaacacgatg ataatatggc cacaaccatg gtgagcaagg gcgaggagct gttcaccggg
     5041 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtct
     5101 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     5161 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     5221 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     5281 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     5341 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     5401 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     5461 tatatcatgg ccgacaagca gaagaacggc atcaaggcga acttcaagat ccgccacaac
     5521 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     5581 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     5641 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     5701 ctcggcatgg acgagctgta caagtaatga attaattaag aattatcaag cttatcgata
     5761 ccgtcgacct gcagccaagc ttatcgataa aataaaagat tttatttagt ctccagaaaa
     5821 aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt
     5881 gcaaggcatg gaaaatacat aactgagaat agagaagttc agatcaaggt taggaacaga
     5941 gagacagcag aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
     6001 gggccaagaa cagatggtcc ccagatgcgg tcccgccctc agcagtttct agagaaccat
     6061 cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc
     6121 aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc
     6181 ccacaacccc tcactcggcg cgccagtcct ccgatagact gcgtcgcccg ggtacccgtg
     6241 tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg
     6301 tctcctctga gtgattgact acccgtcagc gggggtcttt catgggtaac agtttcttga
     6361 agttggagaa caacattctg agggtaggag tcgaatatta agtaatcctg actcaattag
     6421 ccactgtttt gaatccacat actccaatac tcctgaaata gttcattatg gacagcgcag
     6481 aaagagctgg ggagaattgt gaaattgtta tccgctcaca attccacaca acatacgagc
     6541 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     6601 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     6661 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     6721 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     6781 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     6841 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     6901 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     6961 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     7021 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     7081 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     7141 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     7201 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     7261 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     7321 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     7381 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     7441 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     7501 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     7561 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     7621 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     7681 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     7741 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     7801 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     7861 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     7921 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     7981 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     8041 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     8101 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     8161 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     8221 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     8281 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     8341 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     8401 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     8461 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     8521 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     8581 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     8641 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     8701 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     8761 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     8821 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     8881 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     8941 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     9001 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     9061 gttttcccag tcacgacgtt gtaaaacgac ggcgcaagga atggtgcatg caaggagatg
     9121 gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc
     9181 atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca
     9241 gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggcgatta
     9301 gtccaatttg ttaaagacag gatatcagtg gtccaggctc tagttttgac tcaacaatat
     9361 caccagctga agcctataga gtacgagcca tagataaaat aaaagatttt atttagtctc
     9421 cagaaaaagg gggg
//
