LOCUS       sequence-310674-       10235 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses LDB-3 fused with a C-terminal p65 transcriptional 
            activation domain in mammalian cells .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10235)
  AUTHORS   Huang Z, Li Z, Zhang X, Kang S, Dong R, Sun L, Fu X, Vaisar D, 
            Watanabe K, Gu L
  TITLE     Creating Red Light-Switchable Protein Dimerization Systems as 
            Genetically Encoded Actuators with High Specificity.
  JOURNAL   ACS Synth Biol. 2020 Nov 12. doi: 10.1021/acssynbio.0c00397.
  PUBMED    33179507
REFERENCE   2  (bases 1 to 10235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Nov 12. doi: 10.1021/acssynbio.0c00397."
COMMENT     Sequence Label: LDB-3-p65
FEATURES             Location/Qualifiers
     source          1..10235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2391..2507
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2530..2833
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2834..3037
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3034..3058
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3123..3132
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3138..3158
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3174..3194
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4125..4496
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     CDS             4518..4547
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     intron          4636..4865
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     4848..4867
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    5042..5630
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5095..5115)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5513..5524)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6037..6217
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6243..6260)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6249..6473
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6519..6947
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6606..6625)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6816..6837
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6956..6976)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6961..7290
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7141..7276
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7203..7222
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7338..7385
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7404..7778
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7908..8029
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7945..7964)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7999..8018
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8078..8094)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8078..8094)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8091..8113)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8102..8118
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8126..8132,8133..8150,8151..8156))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8171..8192
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8309..8326)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8480..9068)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8560..8579)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9239..10030,10031..10099))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9862..9881
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10100..10204)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcaac ttttaaaaga
     2401 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2461 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gataagcttg
     2521 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2581 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2641 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2701 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2761 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2821 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2881 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2941 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     3001 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     3061 ctggagacgc catccacgct gttttgacct ccatagaaga caccgactga gctcttaagg
     3121 ttgccaccat gggatccccc aagaagaagc gcaaggtgga agctagcgct tccccgaaga
     3181 aaaagcggaa agtcgaggcc tccgcatctc caaaaaaaaa aagcaaggtt gaagcatctg
     3241 gatccggtac cggaggaagt ggcagctctg gcggcagtgg agggtctggt ggcagcggag
     3301 aagttcagct gcaggcaagc ggtggtggtt ttgttcagcc tggtggtagc ctgcgtctga
     3361 gctgtgcagc cagcggtttt acctgggatc attacatcat gggctggttt cgccaggcac
     3421 cgggtaaaga acgtgaattt gttagcgcaa tcagcgaaaa tggtgatgca tggaattatt
     3481 atgccgatag cgtgaaaggt cgctttacca ttagccgtga taatagcaaa aataccgttt
     3541 acctgcagat gaatagtctg cgtgcagaag ataccgcaac ctattattgt gcaatcggtt
     3601 ttgatgttcc atctggtcgt tcttggcagg gttctcattt ttggatgtat tggggtcagg
     3661 gcacccaggt taccgttagc agcagcggcg gcggtggcag tcagtacctg ccagatacag
     3721 acgatcgtca ccggattgag gagaaacgta aaaggacata tgagaccttc aagagcatca
     3781 tgaagaagag tcctttcagc ggacccaccg acccccggcc tccacctcga cgcattgctg
     3841 tgccttcccg cagctcagct tctgtcccca agccagcacc ccagccctat ccctttacgt
     3901 catccctgag caccatcaac tatgatgagt ttcccaccat ggtgtttcct tctgggcaga
     3961 tcagccaggc ctcggccttg gccccggccc ctccccaagt cctgccccag gctccagccc
     4021 ctgcccctgc tccagccatg gtatcagctc tggcccaggc cccagcccct gtcccagtcc
     4081 tagccccagg ccctcctcag gctgtggccc cacctgcccc caagcccacc caggctgggg
     4141 aaggaacgct gtcagaggcc ctgctgcagc tgcagtttga tgatgaagac ctgggggcct
     4201 tgcttggcaa cagcacagac ccagctgtgt tcacagacct ggcatccgtc gacaactccg
     4261 agtttcagca gctgctgaac cagggcatac ctgtggcccc ccacacaact gagcccatgc
     4321 tgatggagta ccctgaggct ataactcgcc tagtgacagg ggcccagagg ccccccgacc
     4381 cagctcctgc tccactgggg gccccggggc tccccaatgg cctcctttca ggagatgaag
     4441 acttctcctc cattgcggac atggacttct cagccctgct gagtcagatc agctccctcg
     4501 agggtggtgg ttccggtgag cagaagctga tctcagagga ggacctgtga tcgagccatg
     4561 gaagcttgat atctaactga ctgaaccggt ggtaccgagg aattaattcg ctgtctgcga
     4621 gggccagctg ttggggtgag tactccctct caaaagcggg catgacttct gcgctaagat
     4681 tgtcagtttc caaaaacgag gaggatttga tattcacctg gcccgcggtg atgcctttga
     4741 gggtggccgc gtccatctgg tcagaaaaga caatcttttt gttgtcaagc ttgaggtgtg
     4801 gcaggcttga gatctggcca tacacttgag tgacaatgac atccactttg cctttctctc
     4861 cacaggtgtc cactcccagg tccaactgca ggtcgagcat gcatctaggg cggccaattc
     4921 cgccgatcct ggaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg
     4981 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcaga
     5041 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     5101 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     5161 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     5221 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     5281 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     5341 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     5401 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     5461 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     5521 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     5581 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattcg
     5641 agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     5701 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat
     5761 ctgtggatct accacacaca aggctacttc cctgattggc agaactacac accagggcca
     5821 gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag
     5881 aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat
     5941 gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt
     6001 catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc
     6061 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     6121 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     6181 ctcagaccct tttagtcagt gtggaaaatc tctagcaggg ccgctttaaa cccgctgatc
     6241 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     6301 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     6361 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     6421 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     6481 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     6541 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     6601 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     6661 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     6721 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     6781 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     6841 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     6901 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     6961 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     7021 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     7081 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     7141 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     7201 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     7261 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     7321 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat
     7381 acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg
     7441 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact
     7501 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg
     7561 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg
     7621 agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg
     7681 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg
     7741 gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt
     7801 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     7861 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     7921 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     7981 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     8041 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     8101 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     8161 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     8221 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     8281 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     8341 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     8401 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     8461 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     8521 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     8581 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     8641 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     8701 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     8761 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     8821 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     8881 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     8941 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     9001 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     9061 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     9121 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     9181 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     9241 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     9301 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     9361 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     9421 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     9481 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     9541 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     9601 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     9661 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     9721 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     9781 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     9841 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     9901 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     9961 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    10021 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    10081 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    10141 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    10201 cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
