LOCUS       sequence-310682-       10904 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses DrBphP fused with an N-terminal GAL4 DNA binding domain in
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10904)
  AUTHORS   Huang Z, Li Z, Zhang X, Kang S, Dong R, Sun L, Fu X, Vaisar D, 
            Watanabe K, Gu L
  TITLE     Creating Red Light-Switchable Protein Dimerization Systems as 
            Genetically Encoded Actuators with High Specificity.
  JOURNAL   ACS Synth Biol. 2020 Nov 12. doi: 10.1021/acssynbio.0c00397.
  PUBMED    33179507
REFERENCE   2  (bases 1 to 10904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Nov 12. doi: 10.1021/acssynbio.0c00397."
COMMENT     Sequence Label: GAL4-DrBphP
FEATURES             Location/Qualifiers
     source          1..10904
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2391..2507
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2530..2833
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2834..3037
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3034..3058
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3123..3132
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3138..3578
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     primer_bind     3589..3608
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3589..3607
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3627..3656
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5187..5216
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     intron          5305..5534
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     5517..5536
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    5711..6299
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5764..5784)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6182..6193)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6706..6886
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6912..6929)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6918..7142
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7188..7616
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7275..7294)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7485..7506
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7625..7645)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7630..7959
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7810..7945
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7872..7891
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8007..8054
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8073..8447
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8577..8698
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8614..8633)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8668..8687
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8747..8763)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8747..8763)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8760..8782)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8771..8787
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8795..8801,8802..8819,8820..8825))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8840..8861
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8978..8995)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9149..9737)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9229..9248)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9908..10699,10700..10768))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10531..10550
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10769..10873)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcaac ttttaaaaga
     2401 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2461 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gataagcttg
     2521 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2581 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2641 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2701 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2761 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2821 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2881 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2941 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     3001 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     3061 ctggagacgc catccacgct gttttgacct ccatagaaga caccgactga gctcttaagg
     3121 ttgccaccat gggatccatg aagctactgt cttctatcga acaagcatgc gatatttgcc
     3181 gacttaaaaa gctcaagtgc tccaaagaaa aaccgaagtg cgccaagtgt ctgaagaaca
     3241 actgggagtg tcgctactct cccaaaacca aaaggtctcc gctgactagg gcacatctga
     3301 cagaagtgga atcaaggcta gaaagactgg aacagctatt tctactgatt tttcctcgag
     3361 aagaccttga catgattttg aaaatggatt ctttacagga tataaaagca ttgttaacag
     3421 gattatttgt acaagataat gtgaataaag atgccgtcac agatagattg gcttcagtgg
     3481 agactgatat gcctctaaca ttgagacagc atagaataag tgcgacatca tcatcggaag
     3541 agagtagtaa caaaggtcaa agacagttga ctgtatcgcc ggaatttgta atacgactca
     3601 ctatagggcg agccgccatc atggaggagc agaagctgat ctcagaggag gacctgcata
     3661 tgagtcgtga ccctttgcca ttctttcctc ctctttatct gggtggaccc gagattacaa
     3721 cagaaaactg cgaacgcgaa ccaattcaca tcccgggatc tattcaacca cacggtgcat
     3781 tgctgacggc agacggacat tccggagagg ttttacagat gtcgcttaac gcagcaacgt
     3841 ttctgggaca agagcctacg gttttgcgtg gccagacgtt agcggctctg ttgccagagc
     3901 aatggccggc cttacaggcg gcattgcctc cagggtgccc cgatgcattg caataccgcg
     3961 cgacactgga ttggccggcg gcaggacatc tttctctgac agtccaccgc gtgggcgagc
     4021 tgttgatcct ggagtttgaa cctacggagg cctgggactc gactggcccg cacgcgttac
     4081 gcaatgcgat gttcgctctt gaatcagcgc caaacttgcg cgcgttagct gaagtggcca
     4141 cacaaaccgt acgcgagctt acaggctttg accgcgtgat gttatacaaa ttcgcacccg
     4201 atgcgacagg cgaggtaatc gccgaagccc gccgcgaggg gttgcatgcc tttcttggcc
     4261 atcgttttcc ggcctcagat attcccgccc aagcgcgcgc cctttacact cgccatctgc
     4321 ttcgtttgac tgcggacacg cgcgcggcgg ccgttccctt agacccagta cttaatcctc
     4381 agaccaacgc tcctaccccc ttagggggtg cagtgctgcg tgcgacgtcg cctatgcaca
     4441 tgcagtacct tcgcaatatg ggcgtcggct cctctttaag tgtatcagtg gtagttgggg
     4501 ggcagttatg gggtctgatt gcgtgccatc atcagacccc ctatgttttg ccaccagacc
     4561 ttcgtactac tcttgaatac ttggggcgtt tattaagcct tcaggtgcaa gtcaaggaag
     4621 ccgcggacgt tgctgcattc cgtcagtcac ttcgcgaaca ccatgcgcgc gtcgccttag
     4681 cggcagcgca ttccctgtcg ccgcacgata ctctttccga ccctgcactt gatcttctgg
     4741 gtctgatgcg tgctgggggc ttaatcctgc gttttgaagg tcgttggcag acgttaggag
     4801 aagtcccgcc cgctcccgca gtcgatgcac tgcttgcatg gcttgaaacc caaccagggg
     4861 cgcttgttca gactgatgca ttggggcagt tgtggccggc gggggctgat ttggctccct
     4921 cagccgcggg tctgcttgcc atttcagtag gggagggatg gagtgagtgc ttggtttggt
     4981 tacgtcccga actgcgcctt gaggttgcgt ggggtggagc aactccagac caggccaagg
     5041 acgacctggg ccctcgtcac agtttcgata cttacttaga agagaagcgt gggtatgcag
     5101 aaccctggca tcccggagag attgaggaag ctcaggattt gcgcgacact cttactggcg
     5161 cattactcga gggtggtggt tccggtgagc agaagctgat ctcagaggag gacctgtgat
     5221 cgagccatgg aagcttgata tctaactgac tgaaccggtg gtaccgagga attaattcgc
     5281 tgtctgcgag ggccagctgt tggggtgagt actccctctc aaaagcgggc atgacttctg
     5341 cgctaagatt gtcagtttcc aaaaacgagg aggatttgat attcacctgg cccgcggtga
     5401 tgcctttgag ggtggccgcg tccatctggt cagaaaagac aatctttttg ttgtcaagct
     5461 tgaggtgtgg caggcttgag atctggccat acacttgagt gacaatgaca tccactttgc
     5521 ctttctctcc acaggtgtcc actcccaggt ccaactgcag gtcgagcatg catctagggc
     5581 ggccaattcc gccgatcctg gaaaaacatg gagcaatcac aagtagcaat acagcagcta
     5641 ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca
     5701 cacctcagac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     5761 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     5821 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     5881 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     5941 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     6001 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     6061 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     6121 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     6181 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     6241 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     6301 tggaattcga gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     6361 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     6421 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca
     6481 ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     6541 gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg
     6601 agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc
     6661 ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta
     6721 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     6781 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     6841 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc cgctttaaac
     6901 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     6961 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     7021 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     7081 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     7141 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     7201 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     7261 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     7321 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     7381 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     7441 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     7501 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     7561 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     7621 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     7681 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     7741 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     7801 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     7861 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     7921 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     7981 tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca
     8041 tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg
     8101 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
     8161 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
     8221 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
     8281 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
     8341 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
     8401 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag
     8461 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     8521 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     8581 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     8641 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     8701 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     8761 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     8821 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     8881 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     8941 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     9001 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     9061 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     9121 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     9181 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     9241 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     9301 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     9361 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     9421 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     9481 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     9541 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     9601 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     9661 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     9721 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     9781 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     9841 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     9901 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     9961 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
    10021 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
    10081 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
    10141 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
    10201 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    10261 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    10321 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    10381 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    10441 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    10501 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    10561 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    10621 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    10681 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    10741 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    10801 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    10861 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
