LOCUS       sequence-310689-        2394 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cytoplasmic expression of a near-infrared genetically encoded 
            calcium indicator iGECI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Shemetov AA, Monakhov MV, Zhang Q, Canton-Josh JE, Kumar M, Chen M, 
            Matlashov ME, Li X, Yang W, Nie L, Shcherbakova DM, Kozorovitskiy Y,
            Yao J, Ji N, Verkhusha VV
  TITLE     A near-infrared genetically encoded calcium indicator for in vivo 
            imaging.
  JOURNAL   Nat Biotechnol. 2021 Mar;39(3):368-377. doi: 
            10.1038/s41587-020-0710-1. Epub 2020 Oct 26.
  PUBMED    33106681
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0710-1"; journalName: "Nat Biotechnol"; date: 
            "2021-03"; volume: "39"; issue: "3"; pages: "368-377"
COMMENT     Sequence Label: piGECI-N1
FEATURES             Location/Qualifiers
     source          1..2394
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1399..1476
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
ORIGIN
        1 atggcggaag gctccgtcgc caggcagcct gacctcttga cctgcgaaca tgaagagatc
       61 cacctcgccg gctcgatcca gccgcatggc gcgcttctgg tcgtcagcga acatgatcat
      121 cgcgtcatcc aggccagcgc caacgccgcg gaatttctga atctcggaag cgtactcggc
      181 gttccgctcg ccgagatcga cggcgatctg ttgatcaaga tcctgccgca tctcgatccc
      241 accgccgaag gcatgccggt cgcggtgcgc tgccggatcg gcaatccctc tacggagtac
      301 tgcggtctga tgcatcggcc tccggaaggc gggctgatca tcgaactcga acgtgccggc
      361 ccgtcgatcg atctgtcagg cacgctggcg ccggcgctgg agcggatccg cacggcgggt
      421 tcactgcgcg cgctgtgcga tgacaccgtg ctgctgtttc agcagtgcac cggctacgac
      481 cgggtgatgg tgtatcgttt cgatgagcaa ggccacggcc tggtattctc cgagtgccat
      541 gtgcctgggc tcgaatccta tttcggcaac cgctatccgt cgtcgactgt cccgcagatg
      601 gcgcggcagc tgtacgtgcg gcagcgcgtc cgcgtgctgg tcgacgtcac ctatcagccg
      661 gtgccgctgg agccgcggct gtcgccgctg accgggcgcg atctcgacat gtcgggctgc
      721 ttcctgcgct cgatgtcgcc gtgccatctg cagttcctga aggacatggg cgtgcgcgcc
      781 accctggcgg tgtcgctggt ggtcggcggc aagctgtggg gcctggttgt ctgtcaccat
      841 tatctgccgc gcttcatccg tttcgagctg cgggcgatct gcaaacggct cgccgaaagg
      901 atcgcgacgc ggatcaccgc gctttacacc catgaccaac tgacagaaga gcagattgca
      961 gagttcaaag aagccttctc attattcgac aaggatgggg acggcaccat caccacaaag
     1021 gaacttggca ccgttatgag gtcgcttgga caaaacccaa cggaagcaga attgcaggat
     1081 atgatcaatg aagtcgatgc tgatggcaat ggaacgattt actttcctga atttcttact
     1141 atgatggcta gaaaaatgaa ggacacagac agcgaagagg aaatccgaga agcattccgt
     1201 gtttttgaca aggatgggaa cggctacatc agcgctgctg aattacgtca cgtcatgaca
     1261 aacctcgggg agaagttaac agatgaagaa gttgatgaaa tgataaggga agcagatatc
     1321 gatggtgatg gccaagtaaa ctatgaagag tttgtacaaa tgatgacagc aaaggggggg
     1381 ggtggcggag gtggctccaa gaggcgctgg aagaaaaact tcattgccgt cagcgctgcc
     1441 aaccggttca agaagatctc cagctccggg gcactggtag taacctgcga cgatgagccg
     1501 atccatatcc ccggtgccat ccaaccgcat ggactgctgc tcgccctcgc cgccgacatg
     1561 acgatcgttg ccggcagcga caaccttccc gaactcaccg gactggcgat cggcgccctg
     1621 atcggccgct ctgcggccga tgtcttcgac tcggagacgc acaaccgtct gacgatcgcc
     1681 ttggccgagc ccggggcggc cgtcggagca ccgatcactg tcggcttcac gatgcgaaag
     1741 gacgcaggct tcatcggctc ctggcatcgc catgatcagc tcatcttcct cgagctcgag
     1801 cctccccagc gggacgtcgc cgagccgcag gcgttcttcc gccgcaccaa cagcgccatc
     1861 cgccgcctgc aggccgccga aaccttggaa agcgcctgcg ccgccgcggc gcaagaggtg
     1921 cggaagatta ccggcttcga tcgggtgatg atctatcgct tcgcctccga cttcagcggg
     1981 tccgtgatcg cagaggatcg gtgcgccgag gtcgagtcaa aactaggcct gcactatcct
     2041 gcctcattca tcccggcgca ggcccgtcgg ctctatacca tcaacccggt acggatcatt
     2101 cccgatatca attatcggcc ggtgccggtc accccagacc tcaatccggt caccgggcgg
     2161 ccgattgatc ttagcttcgc catcctgcgc agcgtctcgc ccaaccatct ggagttcatg
     2221 cgcaacatag gcatgcacgg cacgatgtcg atctcgattt tgcgcggcga gcgactgtgg
     2281 ggattgatcg tttgccatca ccgaacgccg tactacgtcg atctcgatgg ccgccaagcc
     2341 tgcaagaggg tcgccgagag gctggccact cagatcggcg tgatggaaga gtga
//
