LOCUS       sequence-310702-        2008 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of c-luciferase from sp. Vargula tsujii (USA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Hensley NM, Ellis EA, Leung NY, Coupart J, Mikhailovsky A, Taketa 
            DA, Tessler M, Gruber DF, De Tomaso AW, Mitani Y, Rivers TJ, Gerrish
            GA, Torres E, Oakley TH
  TITLE     Selection, drift, and constraint in cypridinid luciferases and the 
            diversification of bioluminescent signals in sea fireflies.
  JOURNAL   Mol Ecol. 2020 Oct 8. doi: 10.1111/mec.15673.
  PUBMED    33031624
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ecol. 
            2020 Oct 8. doi: 10.1111/mec.15673."
COMMENT     Sequence Label: pPICZ-aC-VTS-US
FEATURES             Location/Qualifiers
     source          1..2008
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..57,58..255)
                     /codon_start=1
                     /gene="MF-alpha-1"
                     /product="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
                     /label=alpha-factor secretion signal
                     /note="Cleavage by the Kex2 protease occurs after the 
                     dibasic KR sequence."
                     /translation="MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDLE
                     GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKR"
     CDS             1888..1917
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1933..1950
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgagatttc cttcaatttt tactgctgtt ttattcgcag catcctccgc attagctgct
       61 ccagtcaaca ctacaacaga agatgaaacg gcacaaattc cggctgaagc tgtcatcggt
      121 tactcagatt tagaagggga tttcgatgtt gctgttttgc cattttccaa cagcacaaat
      181 aacgggttat tgtttataaa tactactatt gccagcattg ctgctaaaga agaaggggta
      241 tctctcgaga agagagaggc tcaagattgt tatgaatcaa catgggcttc gaatgattat
      301 ccatcttcat gtgaggctct aaacggaaga tgtgtagact ctgcgtgcgg cagctgtgac
      361 gaggttctgt ttggtgatgg attgtgtgaa aatgcaggag gagcgtctcc caagtgctgt
      421 agacagcttg attgcccaga aatcgtcaga tgcagagttt cagcagctgg atacttccaa
      481 acattctatg ggcagagatt caatctccag gtaccaggaa cctatctctt gagtgaagac
      541 tgcgtgggag gactatggtc tctatacgtt aacctggcga atattgaagg agagaaggga
      601 gcagttttgg attccgtcaa gatggttgtt ggagacgtaa ctgtcgatat taaacagaaa
      661 gttggagctg ttacagtaaa tggaggttcc gttgaaatcg acagtaaccc attctctatt
      721 ggtgacgtca ctattgccat cgtacacact cctaactttg atgtctccgt catcgagttc
      781 cttaagcttg ttacattcga tatcttacaa ggcagagctt tcagacttgc tccagatttc
      841 ttatacgcag acaggacctg tggtttatgt ggagtcatgt ccgatgaacc tactgatttt
      901 attgacaatc cagatcagct tgcaatccag gaccaaatga atcaagatat agacggctgc
      961 ccactctctg gaaatccttc tgacgtcgaa tattgcgtga ataagatgca gccatataag
     1021 gacgggtgtg ttaacaagaa cgatgtccac ttcggaacct acctgtacgc atgcgcactg
     1081 gcttactgca tgggcggcga cgacagagtg gaggatgtta tatttgaata cgccgaagcc
     1141 tgcgttgaac ccatcggtag agcaacctgc gtcatgaatg gacacacata ctacgataca
     1201 ttcgacaaaa caagttatca gttccaggca ccttgcaagg ttctattcgc caaagactgt
     1261 acagatgacg attgggaagt aaccattact cataaagcag ttggagaata tactgaagta
     1321 gagaaggtca ccgtccggta ttttcaaacc ttgattgacc tgatatcgga gactaagaaa
     1381 gttttcgtta atggaacgga agtgagcgtc ccttacaact atggagatac atctatatac
     1441 atgtatgata acttaataac tacagcagtt ctaccaggag cagtggtggt gaaatacaac
     1501 ttcgagcaga tgttggctct acacatcaga gatccatatg aagcagacag ctgtggtctg
     1561 tgtggtatct gggatttgga taaatctaat gagggtcctg atacccagta tgtagattgt
     1621 gaaccaactc ccaaccctcc aacatgtact gctgacaaag aggctgaagc aagggaactc
     1681 tgtcagaata tgttccctgc ttctctcgac gaccaatgta acatctgcta caaggctgac
     1741 cgtgttgagc gctgcatgta tgagtattgt cttggtggtc tggagggctt ctgtcaacac
     1801 gctggtaccg tcatcgatga gtgcttcgtg agacacggag acgatctaca gtatcctcct
     1861 cagtgtaaag cggccgccag ctttctagaa caaaaactca tctcagaaga ggatctgaat
     1921 agcgccgtcg accatcatca tcatcatcat tgagtttgta gccttagaca tgactgttcc
     1981 tcagttcaag tngggcactt acgagaag
//
