LOCUS       sequence-310706-        1987 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of c-luciferase from sp. Kornikeria hastingsi carriebowae
            (Belize).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Hensley NM, Ellis EA, Leung NY, Coupart J, Mikhailovsky A, Taketa 
            DA, Tessler M, Gruber DF, De Tomaso AW, Mitani Y, Rivers TJ, Gerrish
            GA, Torres E, Oakley TH
  TITLE     Selection, drift, and constraint in cypridinid luciferases and the 
            diversification of bioluminescent signals in sea fireflies.
  JOURNAL   Mol Ecol. 2020 Oct 8. doi: 10.1111/mec.15673.
  PUBMED    33031624
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ecol. 
            2020 Oct 8. doi: 10.1111/mec.15673."
COMMENT     Sequence Label: pPICZ-aC-KHC-BZ
FEATURES             Location/Qualifiers
     source          1..1987
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..57,58..255)
                     /codon_start=1
                     /gene="MF-alpha-1"
                     /product="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
                     /label=alpha-factor secretion signal
                     /note="Cleavage by the Kex2 protease occurs after the 
                     dibasic KR sequence."
                     /translation="MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDLE
                     GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKR"
     CDS             1885..1914
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1930..1947
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgagatttc cttcaatttt tactgctgtt ttattcgcag catcctccgc attagctgct
       61 ccagtcaaca ctacaacaga agatgaaacg gcacaaattc cggctgaagc tgtcatcggt
      121 tactcagatt tagaagggga tttcgatgtt gctgttttgc cattttccaa cagcacaaat
      181 aacgggttat tgtttataaa tactactatt gccagcattg ctgctaaaga agaaggggta
      241 tctctcgaga agagagaggc taaagattgt tttgaatcat ctttccacag cctagaattt
      301 ccttcgtcat gtgaggcaca aaatggcatc tgtattgata gcgagtgtaa agactgtaat
      361 gaagttatgt ttggcgatgg attgtgcgaa aatgccggag gagcctctcc aaagtgctgt
      421 agagattgtc cggaaattgt tagatgcagg gcttcagcag ctgggttctt tacaacgttt
      481 tatggaaaga agttcaatct tcaggaacca ggaacctatc tgctaagtga agactgcgtc
      541 ggaggcctgt ggtctttata tgttacgctg gtgaatatag caggagaaaa gggtgctgtt
      601 ctgggatccg taaagatgat tgttggagaa gtaacggtag atattgtaca gaaaggagga
      661 cccgtcacag taaatggagg ttctgttgca atcgacagca atcctttctc tattggtgac
      721 gtcactattg ccatcgtaca cacacctaac ttcgacgttg ctgttataga gtttcttaag
      781 ctggttacct ttgatatcat ccatggaaga gctttccgac tagctcccga tttcctgtac
      841 cgagatagga cctgtggttt gtgtggcgtg atgtcaaatg agccgactga ttttattgat
      901 aatccggatc agctagcagt ccaagataag atcaacaaag atatagatgg ttgtcctctt
      961 tctggaaatc cttctgatgt cgaatattgc aaaaataaga tgcagccata caaggtcgct
     1021 tgtataaact acaatgacgt caatttcgct acttacctat attcctgcgc cttggcctac
     1081 tgtatgggcg gggaggacag agtagaagac gtcatatttg attatgtaga agcatgcgta
     1141 gaaccaataa cgagagctac atgtgttatg aatggacaca catattatga cacattcgac
     1201 aaaacaagtt atcaatttca ggcaccatgc aaggttttat tcgctaaaga ctgcgcagga
     1261 gatgaatggg aagtgacaat tactcataaa gcggctggaa catacacaga agtagagaag
     1321 gtcaccgtcc gatatttcca aacgttaata gatttgatat cagaaggtaa acaggttctg
     1381 gtaaacggaa cagaagtaag tgtaccttac aataaaggag atacgtccat ctacatgtac
     1441 gacaatctga taacaacagc ggttctacca ggggcagtgg tcgtaaaata caactttgag
     1501 caaatgcttg cgctgcacat aagagatcca tacgaaagaa ggagctgtgg cttatgtgga
     1561 atttgggatt tggataaatc taacgatggt cctgacaacc agtatgtaga ctgtgaacca
     1621 acccccaacc ctgcaacctg cactgctgac caagaggcag aagccagaga actatgtcag
     1681 aacatgtttc ctgcttcgct cgatgatgaa tgtgacatct gctacaagtc tgaccgcgtg
     1741 gagcggtgca tgtacgagta ttgccttggt ggaatggatt acttctgtaa gcacgcaggt
     1801 accgtcattg atgaatgctt cgtgagacac ggagatgatc tacaattacc acctcaatgc
     1861 accaaagcgg ccgccagctt tctagaacaa aaactcatct cagaagagga tctgaatagc
     1921 gccgtcgacc atcatcatca tcatcattga gtttgtagcc ttagacatga ctgttcctca
     1981 gttcaag
//
